WO2010005273A3 - Transgenic zebrafish and method for mass production of human lactoferrin using the same - Google Patents

Transgenic zebrafish and method for mass production of human lactoferrin using the same Download PDF

Info

Publication number
WO2010005273A3
WO2010005273A3 PCT/KR2009/003832 KR2009003832W WO2010005273A3 WO 2010005273 A3 WO2010005273 A3 WO 2010005273A3 KR 2009003832 W KR2009003832 W KR 2009003832W WO 2010005273 A3 WO2010005273 A3 WO 2010005273A3
Authority
WO
WIPO (PCT)
Prior art keywords
human lactoferrin
transgenic zebrafish
mass production
same
zebrafish
Prior art date
Application number
PCT/KR2009/003832
Other languages
French (fr)
Korean (ko)
Other versions
WO2010005273A2 (en
Inventor
박재학
석승혁
조영식
하건우
오진식
오연경
Original Assignee
서울대학교산학협력단
주식회사 바이오노트
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 서울대학교산학협력단, 주식회사 바이오노트 filed Critical 서울대학교산학협력단
Publication of WO2010005273A2 publication Critical patent/WO2010005273A2/en
Publication of WO2010005273A3 publication Critical patent/WO2010005273A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/79—Transferrins, e.g. lactoferrins, ovotransferrins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A01K2217/052—Animals comprising random inserted nucleic acids (transgenic) inducing gain of function
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/40—Fish
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Veterinary Medicine (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to zebrafish that mass produce human lactoferrin after being transformed with a recombinant expression vector containing the human lactoferrin gene, to a method for the mass production of human lactoferrin from said transgenic zebrafish, and to functional foods and animal feed additives containing said transgenic zebrafish as the active ingredient. According to the present invention, a large quantity of human lactoferrin can be obtained from transgenic zebrafish. Furthermore, the transgenic zebrafish can be used as an ingredient for functional foods and as an additive for animal feed.
PCT/KR2009/003832 2008-07-11 2009-07-13 Transgenic zebrafish and method for mass production of human lactoferrin using the same WO2010005273A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2008-0067786 2008-07-11
KR1020080067786A KR20100007241A (en) 2008-07-11 2008-07-11 A transgenic zebrafish for mass production of human lactoferrin and a process of producing human lactoferrin using the same

Publications (2)

Publication Number Publication Date
WO2010005273A2 WO2010005273A2 (en) 2010-01-14
WO2010005273A3 true WO2010005273A3 (en) 2010-03-25

Family

ID=41507605

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/003832 WO2010005273A2 (en) 2008-07-11 2009-07-13 Transgenic zebrafish and method for mass production of human lactoferrin using the same

Country Status (2)

Country Link
KR (1) KR20100007241A (en)
WO (1) WO2010005273A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20160012735A (en) * 2014-07-25 2016-02-03 한국생명공학연구원 Dyrk1aa mutant zebrafish model for vascular disease and screening method of vascular disease treatment agent using the same
KR101833971B1 (en) * 2016-02-19 2018-03-02 사회복지법인 삼성생명공익재단 Pharmaceutical composition for prevention or treatment of muscular disease containing mesenchymal stem cells or XCL1

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020013955A1 (en) * 1998-06-10 2002-01-31 Sharon Ogden Production of recombinant protein in transgenic fish
US20060179506A1 (en) * 2005-01-07 2006-08-10 Academia Sinica In vivo assay for neurite outgrowth in zebrafish and its application in drug screening

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020013955A1 (en) * 1998-06-10 2002-01-31 Sharon Ogden Production of recombinant protein in transgenic fish
US20060179506A1 (en) * 2005-01-07 2006-08-10 Academia Sinica In vivo assay for neurite outgrowth in zebrafish and its application in drug screening

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HAN ET AL.: "High-level expression of human lactoferrin in the milk of goats by using replication-defective adenoviral vectors", PROTEIN EXPRESSION AND PURIFICATION, vol. 53, 2007, pages 225 - 231 *
ROCHA ET AL.: "Application of Inducible and Targeted Gene Strategies to Produce Transgenic Fish: A Review", MAR. BIOTECHNOL., vol. 6, 2004, pages 118 - 127 *

Also Published As

Publication number Publication date
WO2010005273A2 (en) 2010-01-14
KR20100007241A (en) 2010-01-22

Similar Documents

Publication Publication Date Title
WO2013029013A8 (en) Enzyme producing bacillus strains
MX2013010631A (en) N-(3-carbamoylphenyl)-1h-pyrazole-5-carboxamide derivatives and the use thereof for controlling animal pests.
WO2009126933A3 (en) Site-specific delivery of nucleic acids by combining targeting ligands with endosomolytic components
SG10201811116UA (en) Non-human animals having a humanized programmed cell death 1 gene
WO2012116274A3 (en) Genetically modified animals and methods for making the same
MX2012013897A (en) N-[(het)arylethyl)] pyrazole(thio)carboxamides and their heterosubstituted analogues.
MX2015013033A (en) Method of preparing highly dispersible whole grain flour with an increased avenanthramide content.
WO2013060867A3 (en) Production of heterodimeric proteins
WO2007106452A3 (en) Methods and compositions for increased productivity in animals
WO2009129465A3 (en) Compositions and methods for inhibiting expression of xbp-1 gene
MX2016014944A (en) Palatability enhancers comprising amino reactants and carbonyl compounds for use in cat food.
BR112015031719A2 (en) method for preparing feed additive
WO2013119468A3 (en) Improvement of stability of phytase with phytic acid, and compositions|comprising phytase and phytic acid
MX2013012154A (en) Synthetic phytase variants.
WO2011048046A3 (en) Synthetic phytase variants
WO2010068897A3 (en) Stem cell enhanced protein products and uses thereof
MX2010004642A (en) Method for reducing the saturated fatty acid content of milk fat, products obtained and applications thereof.
MX2013012153A (en) Synthetic phytase variants.
PL2793607T3 (en) Ingredients for animal feed compositions
MX2018009513A (en) Non-human animals having an engineered angptl8 gene.
MX2015015127A (en) Animal feed enzymes.
WO2011000933A3 (en) Enhanced phytase variants
MX2015014121A (en) Feed product and/or feed ingredient.
MY158209A (en) Preparation of feed compositions
EA201391677A1 (en) APPLICATION OF EMULSORS TOGETHER WITH PLANT OLEINS FOR ANIMALS

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09794679

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 09794679

Country of ref document: EP

Kind code of ref document: A2