WO2010004345A1 - Piperidinyl gpcr agonists - Google Patents

Abstract

Compounds of formula (I): or pharmaceutically acceptable salts thereof, are GPCR agonists and are useful as for the treatment of diabetes and obesity.

Description

PIPERIDINYL GPCR AGONISTS

BACKGROUND OF THE INVENTION

The present invention is directed to G-protein coupled receptor (GPCR) agonists. In particular, the present invention is directed to agonists of GPRl 19 that are useful for the treatment of obesity, e.g. as regulators of satiety, metabolic syndrome and for the treatment of diabetes.

Obesity is characterized by an excessive adipose tissue mass relative to body size. Clinically, body fat mass is estimated by the body mass index (BMI; weight(kg)/height(m)²), or waist circumference. Individuals are considered obese when the BMI is greater than 30 and there are established medical consequences of being overweight. It has been an accepted medical view for some time that an increased body weight, especially as a result of abdominal body fat, is associated with an increased risk for diabetes, hypertension, heart disease, and numerous other health complications, such as arthritis, stroke, gallbladder disease, muscular and respiratory problems, back pain and even certain cancers.

Pharmacological approaches to the treatment of obesity have been mainly concerned with reducing fat mass by altering the balance between energy intake and expenditure. Many studies have clearly established the link between adiposity and the brain circuitry involved in the regulation of energy homeostasis. Direct and indirect evidence suggest that serotonergic, dopaminergic, adrenergic, cholinergic, endocannabinoid, opioid, and histaminergic pathways in addition to many neuropeptide pathways (e.g. neuropeptide Y and melanocortins) are implicated in the central control of energy intake and expenditure. Hypothalamic centres are also able to sense peripheral hormones involved in the maintenance of body weight and degree of adiposity, such as insulin and leptin, and fat tissue derived peptides.

Drugs aimed at the pathophysiology associated with insulin dependent Type I diabetes and non-insulin dependent Type II diabetes have many potential side effects and do not adequately address the dyslipidaemia and hyperglycaemia in a high proportion of patients. Treatment is often focused at individual patient needs using diet, exercise, hypoglycaemic agents and insulin, but there is a continuing need for novel antidiabetic agents, particularly ones that may be better tolerated with fewer adverse effects.

Similarly, metabolic syndrome (syndrome X) places people at high risk of coronary artery disease, and is characterized by a cluster of risk factors including central obesity (excessive fat tissue in the abdominal region), glucose intolerance, high triglycerides and low HDL cholesterol, and high blood pressure. Myocardial ischemia and microvascular disease is an established morbidity associated with untreated or poorly controlled metabolic syndrome.

There is a continuing need for novel antiobesity and antidiabetic agents, particularly ones that are well tolerated with few adverse effects.

GPRl 19 (previously referred to as GPRl 16) is a GPCR identified as SNORF25 in WO00/50562 which discloses both the human and rat receptors, US 6,468,756 also discloses the mouse receptor (accession numbers: AAN95194 (human), AAN95195 (rat) and ANN95196 (mouse)).

In humans, GPRl 19 is expressed in the pancreas, small intestine, colon and adipose tissue. The expression profile of the human GPRl 19 receptor indicates its potential utility as a target for the treatment of obesity and diabetes. International patent applications WO2005/061489, WO2006/070208 and WO2006/067532 disclose heterocyclic derivatives as GPRl 19 receptor agonists. International patent applications WO2006/067531, WO2007/003960, WO2007/003961, WO2007/003962 and WO2007/003964, WO2007/116230 and WO2007/116229 disclose GPRl 19 receptor agonists.

The present invention relates to agonists of GPRl 19 which are useful for the treatment of diabetes and as peripheral regulators of satiety, e.g. for the treatment of obesity and metabolic syndrome.

SUMMARY OF THE INVENTION

Compounds of formula (I):

(I) or pharmaceutically acceptable salts thereof, are agonists of GPRl 19 and are useful for the prophylactic or therapeutic treatment of diabetes and obesity.

DETAILED DESCRIPTION OF THE INVENTION

The present invention is directed to a compound of formula (I), or a pharmaceutically acceptable salt thereof:

(I) wherein one of X and Y is O and the other is N; one of E and Q is N and the other is CR²; R¹ is -SO₂R⁵, -CONHR⁶, oxadiazolyl or triazolyl; R² is hydrogen or methyl; R³ is hydrogen or methyl; R⁴ is C₂-₅alkyl; R⁵ is Ci_₃alkyl; and

R⁶ is hydrogen, Ci_₃alkyl, or C₂_₃alkyl substituted by one or two hydroxy groups. In one embodiment of the invention X is O and in another Y is O. X is preferably O. Y is preferably N. Q is preferably N. E is preferably CR². R² is preferably methyl. In one embodiment of the invention R³ is hydrogen and in another R³ is methyl. When R³ is methyl, the stereocentre produced preferably has the (/^-configuration.

R⁴ is preferably C₂.₄ alkyl, particularly ethyl, w-propyl, isopropyl, or tert-butyl, even more preferably C₃ alkyl, especially isopropyl.

R¹ is preferably -SO₂R⁵, -CONHR⁶, [l,3,4]oxadiazolyl or [l,2,4]triazolyl, more preferably -SO₂R⁵, [l,3,4]oxadiazolyl or [l,2,4]triazolyl, especially -SO₂R⁵.

R⁵ is preferably methyl.

R⁶ is preferably Ci_₃alkyl or C₂_₃alkyl substituted by one or two hydroxy groups, more preferably C₂_₃alkyl substituted by one or two hydroxyl groups, e.g. 2-hydroxyethyl, 2-hydroxy- 1-methylethyl, 2,3-dihydroxypropyl or 2-hydroxy-l-hydroxymethylethyl, preferably 2- hydroxyethyl or 2-hydroxy-l-methylethyl, even more preferably 2-hydroxy-l-methylethyl, especially (/?)-2-hydroxy- 1 -methylethyl.

While the preferred groups for each variable have generally been listed above separately for each variable, preferred compounds of this invention include those in which several or each variable in formula (I) is selected from the preferred, more preferred or particularly listed groups for each variable. Therefore, this invention is intended to include all combinations of preferred, more preferred and particularly listed groups.

Specific compounds of the invention which may be mentioned are those included in the Examples and pharmaceutically acceptable salts thereof.

As used herein, unless stated otherwise, "alkyl" means carbon chains which may be linear or branched or combinations thereof. Examples of alkyl groups include methyl, ethyl, propyl, isopropyl, butyl, sec- and tert-butyl and pentyl.

"Fluoroalkyl" means alkyl groups substituted by one or more fluoro atoms, e.g. CHF₂ and CF₃.

The term "halo" includes fluorine, chlorine, bromine, and iodine atoms, in particular fluorine or chlorine, especially fluorine.

Compounds described herein may contain one or more asymmetric centers and may thus give rise to diastereomers and optical isomers. The present invention includes all such possible diastereomers as well as their racemic mixtures, their substantially pure resolved enantiomers, all possible geometric isomers, and pharmaceutically acceptable salts thereof. The above formula (I) is shown without a definitive stereochemistry at certain positions. The present invention includes all stereoisomers of formula (I) and pharmaceutically acceptable salts thereof. Further, mixtures of stereoisomers as well as isolated specific stereoisomers are also included. During the course of the synthetic procedures used to prepare such compounds, or in using racemization or epimerization procedures known to those skilled in the art, the products of such procedures can be a mixture of stereoisomers.

When the compound of formula (I) and pharmaceutically acceptable salts thereof exist in the form of solvates or polymorphic forms, the present invention includes any possible solvates and polymorphic forms. A type of a solvent that forms the solvate is not particularly limited so long as the solvent is pharmacologically acceptable. For example, water, ethanol, propanol, acetone or the like can be used.

The term "pharmaceutically acceptable salts" refers to salts prepared from pharmaceutically acceptable non-toxic acids, including inorganic and organic acids. Such acids include, for example, hydrochloric, methanesulfonic, sulfuric, p-toluenesulfonic acid and the like.

Since the compounds of formula (I) are intended for pharmaceutical use they are preferably provided in substantially pure form, for example at least 60% pure, more suitably at least 75% pure, especially at least 98% pure (% are on a weight for weight basis).

The compounds of formula (I) can be prepared as described below. PG represents a protecting group, G is a substituted oxadiazole as defined above, and R¹, R², R³ and R⁴, as well as E and Q, are also as defined above.

Compounds of formula (II), where PG is a suitable protecting group can be readily prepared from known compounds (Scheme 1). For example, the ethyl ester of compound (II) where PG is Boc has been previously reported (US Patent 6,518,423). Saponification and hydrogenation under standard conditions will yield the racemic compound of formula (III). Chiral reduction of the alkenoic acid (II) under suitable conditions, such as a hydrogenation in the presence of a chiral catalyst, yields compounds of formula (III) in high enantiomeric excess. An example of a suitable catalyst is [Rh(norbornadiene)₂]BF₄ and (S)-I -[(R)-2-(di-tert- butylphosphino)ferrocenyl]ethylbis(2-methylphenyl)phosphine. Compounds of formula (IV) can then be obtained by reduction of the carboxylic acids of formula (III) under standard conditions, for example borane in a suitable solvent such as THF. Removal of the protecting group is then achieved under conditions well known to those with skill in the art.

Scheme 1

M

Ii m ^IV

The compound of formula (V) where R³ is H is a known compound (Scheme 2, Siegel, M. G. et al. Tetrahedron 1999, 55, 11619-11639). Compounds of formula (XI) can be prepared from compounds of formula (V) under standard conditions. For example, treatment of compounds of formula (V) with cyanogen bromide followed by condensation of the resultant cyanamide (VI) with a compound of formula (IX) under standard conditions yields compounds of formula (VII) where X is O. Compounds of formula (IX) are either commercially available, or readily prepared from the corresponding carboxylic acids using well known techniques. Alternatively, synthesis of the regioisomeric oxadiazole, where Y is O, can be achieved by heating compounds of formula (VI) with hydroxylamine to give N-hydroxyguanidines of formula (VIII) that may be condensed with a carboxylic acid of formula (X) under suitable conditions. Acids of formula (X) are commercially available. Compounds of formula (XI) can be synthesized from compounds of formula (VII) using standard Gabriel amine synthesis conditions.

Scheme 2

XI

Compounds of formula (VII) may also be prepared by condensation of amine (V) with an oxadiazole chloride of formula (XII), as illustrated in Scheme 3 (Buscemi, S. et al. JCS Perkin I: Org. and Bioorg. Chem., 1988, 1313 and Adembri, G, et al. JCS Perkin I: Org. and Bioorg. Chem., 1981, 1703).

Scheme 3

V XII VII

Compounds of formula (I) where R¹ is -SO₂R⁵ can be produced as outlined in Scheme 4. For example, the compound of formula (XIII) where R² is hydrogen is commercially available. Bromine-metal exchange using butyllithium in a suitable solvent such as toluene, in the presence of a chelating agent such as TMEDA if required, at low temperature followed by the addition of dimethyldisulfide yields compounds of formula (XIV) where R⁵ is methyl. Oxidation to the sulfone occurs using a standard oxidizing agent, such as m-CPBA, to give compounds of formula (XV). Compounds of formula (I) are then formed by displacement of the 2-flouro group in compounds of formula (XV) with an amine of formula (XI) under standard conditions.

Scheme 4

Compounds of formula (I) where R¹ is -CONHR⁶ can be produced as outlined in Scheme 5. Compounds of formula (XVI) where Q is nitrogen, E is CR² and X is a leaving group have been previously reported in the literature or can be readily prepared using known techniques. For example, the compound where Q is nitrogen, E is CHMe, Ak is ethyl, and X is chloro has been reported by Ramirez, F., et al. J. Org. Chem., 1954, 19, 183. Compounds of formula (I) are then produced by displacement of the leaving group in compounds of formula (XVI) with an amine of formula (XI) under standard conditions. Hydrolysis of the ester of formula (XVII) under standard conditions, for example using aqueous lithium hydroxide at about room temperature, yields acids of formula (XVIII). Amide bond formation under standard conditions well known to those with skill in the art, yields the desired compounds of formula (I).

Scheme 5

Compounds of formula (I) where R¹ is 1,3,4-oxadiazolyl can be produced as outlined in Scheme 6. Compounds of formula (XVIII) (as synthesized in Scheme 5) can be reacted with hydrazine, using standard amide coupling conditions well known to those with skill in the art, to yield compounds of formula (IX). Subsequent cyclisation with trimethylorthoformate yields compounds of formula (I) as described above.

Scheme 6

Compounds of formula (I) where R¹ is 1 ,2,4-triazole, can be produced as outlined in Scheme 7. Compounds of formula (XX) where one of Q or E is nitrogen and the other is CR² have been previously reported in the literature or can be readily prepared using known techniques. For example, the compound where E is nitrogen and Q is CHMe has been reported in WO08/005569 and the compound where Q is nitrogen and E is CHMe can be readily prepared from 5-iodo-6-methylpyridin-2-ylamine and 1 ,2,4-traizole using standard Buchwald coupling conditions (for example, CuI, K₃PO₄ and N,N'-dimethylethane-l,2-diamine in a suitable solvent such as DMF with heating at 15O⁰C). Reaction of compounds of formula (XX) with aldehydes of formula (XXI) (which can be readily prepared from alcohols of formula (VII)), under standard reductive animation conditions well known to those with skill in the art, yields compounds of formula (I) as described above

Scheme 7

XX XXI

Other compounds of formula (I) may be prepared by methods analogous to those described above or by methods known per se. Further details for the preparation of the compounds of formula (I) are found in the examples.

The compounds of formula (I) may be prepared singly or as compound libraries comprising at least 2, for example 5 to 1,000, compounds and more preferably 10 to 100 compounds of formula (I). Compound libraries may be prepared by a combinatorial "split and mix" approach or by multiple parallel synthesis using either solution or solid phase chemistry, using procedures known to those skilled in the art.

During the synthesis of the compounds of formula (I), labile functional groups in the intermediate compounds, e.g. hydroxy, carboxy and amino groups, may be protected. The protecting groups may be removed at any stage in the synthesis of the compounds of formula (I) or may be present on the final compound of formula (I). A comprehensive discussion of the ways in which various labile functional groups may be protected and methods for cleaving the resulting protected derivatives is given in, for example, Protective Groups in Organic Chemistry, T.W. Greene and P.G.M. Wuts, (1991) Wiley-Interscience, New York, 2^nd edition.

Any novel intermediates, such as those defined above, may be of use in the synthesis of compounds of formula (I) and are therefore also included within the scope of the invention, for example compounds of formulae (XVII) and (XVIII) or a salt or protected derivative thereof. The processes for the production of compounds of formula (I) described above also represent further aspects of the invention.

As indicated above the compounds of formula (I) are useful as GPRl 19 agonists, e.g. for the treatment and/or prophylaxis of obesity and diabetes. For such use the compounds of formula (I) will generally be administered in the form of a pharmaceutical composition.

The invention also provides a compound of formula (I), or a pharmaceutically acceptable salt thereof, for use as a pharmaceutical.

The invention also provides a pharmaceutical composition comprising a compound of formula (I), in combination with a pharmaceutically acceptable carrier.

Preferably the composition is comprised of a pharmaceutically acceptable carrier and a non-toxic therapeutically effective amount of a compound of formula (I), or a pharmaceutically acceptable salt thereof.

Moreover, the invention also provides a pharmaceutical composition for the treatment of disease by modulating GPRl 19, resulting in the prophylactic or therapeutic treatment of obesity, e.g. by regulating satiety, or for the treatment of diabetes, comprising a pharmaceutically acceptable carrier and a non-toxic therapeutically effective amount of compound of formula (I), or a pharmaceutically acceptable salt thereof.

The pharmaceutical compositions may optionally comprise other therapeutic ingredients or adjuvants. The compositions include compositions suitable for oral, rectal, topical, and parenteral (including subcutaneous, intramuscular, and intravenous) administration, although the most suitable route in any given case will depend on the particular host, and nature and severity of the conditions for which the active ingredient is being administered. The pharmaceutical compositions may be conveniently presented in unit dosage form and prepared by any of the methods well known in the art of pharmacy.

In practice, the compounds of formula (I), or pharmaceutically acceptable salts thereof, can be combined as the active ingredient in intimate admixture with a pharmaceutical carrier according to conventional pharmaceutical compounding techniques. The carrier may take a wide variety of forms depending on the form of preparation desired for administration, e.g. oral or parenteral (including intravenous).

Thus, the pharmaceutical compositions can be presented as discrete units suitable for oral administration such as capsules, cachets or tablets each containing a predetermined amount of the active ingredient. Further, the compositions can be presented as a powder, as granules, as a solution, as a suspension in an aqueous liquid, as a non-aqueous liquid, as an oil-in-water emulsion, or as a water-in-oil liquid emulsion. In addition to the common dosage forms set out above, the compound of formula (I), or a pharmaceutically acceptable salt thereof, may also be administered by controlled release means and/or delivery devices. The compositions may be prepared by any of the methods of pharmacy. In general, such methods include a step of bringing into association the active ingredient with the carrier that constitutes one or more necessary ingredients. In general, the compositions are prepared by uniformly and intimately admixing the active ingredient with liquid carriers or finely divided solid carriers or both. The product can then be conveniently shaped into the desired presentation.

The compounds of formula (I), or pharmaceutically acceptable salts thereof, can also be included in pharmaceutical compositions in combination with one or more other therapeutically active compounds. The pharmaceutical carrier employed can be, for example, a solid, liquid, or gas. Examples of solid carriers include lactose, terra alba, sucrose, talc, gelatin, agar, pectin, acacia, magnesium stearate, and stearic acid. Examples of liquid carriers are sugar syrup, peanut oil, olive oil, and water. Examples of gaseous carriers include carbon dioxide and nitrogen.

In preparing the compositions for oral dosage form, any convenient pharmaceutical media may be employed. For example, water, glycols, oils, alcohols, flavoring agents, preservatives, coloring agents, and the like may be used to form oral liquid preparations such as suspensions, elixirs and solutions; while carriers such as starches, sugars, microcrystalline cellulose, diluents, granulating agents, lubricants, binders, disintegrating agents, and the like may be used to form oral solid preparations such as powders, capsules and tablets. Because of their ease of administration, tablets and capsules are the preferred oral dosage units whereby solid pharmaceutical carriers are employed. Optionally, tablets may be coated by standard aqueous or nonaqueous techniques.

A tablet containing the composition of this invention may be prepared by compression or molding, optionally with one or more accessory ingredients or adjuvants. Compressed tablets may be prepared by compressing, in a suitable machine, the active ingredient in a free -flowing form such as powder or granules, optionally mixed with a binder, lubricant, inert diluent, surface active or dispersing agent. Molded tablets may be made by molding in a suitable machine, a mixture of the powdered compound moistened with an inert liquid diluent. Each tablet preferably contains from about 0.05mg to about 5g of the active ingredient and each cachet or capsule preferably containing from about 0.05mg to about 5g of the active ingredient.

For example, a formulation intended for the oral administration to humans may contain from about 0.5mg to about 5g of active agent, compounded with an appropriate and convenient amount of carrier material which may vary from about 5 to about 95 percent of the total composition. Unit dosage forms will generally contain between from about lmg to about 2g of the active ingredient, typically 25 mg, 50mg, lOOmg, 200mg, 300mg, 400mg, 500mg, 600mg, 800mg, or lOOOmg.

Pharmaceutical compositions of the present invention suitable for parenteral administration may be prepared as solutions or suspensions of the active compounds in water. A suitable surfactant can be included such as, for example, hydroxypropylcellulose. Dispersions can also be prepared in glycerol, liquid polyethylene glycols, and mixtures thereof in oils. Further, a preservative can be included to prevent the detrimental growth of microorganisms.

Pharmaceutical compositions of the present invention suitable for injectable use include sterile aqueous solutions or dispersions. Furthermore, the compositions can be in the form of sterile powders for the extemporaneous preparation of such sterile injectable solutions or dispersions. In all cases, the final injectable form must be sterile and must be effectively fluid for easy syringability. The pharmaceutical compositions must be stable under the conditions of manufacture and storage; thus, preferably should be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (e.g. glycerol, propylene glycol and liquid polyethylene glycol), vegetable oils, and suitable mixtures thereof.

Pharmaceutical compositions of the present invention can be in a form suitable for topical use such as, for example, an aerosol, cream, ointment, lotion, dusting powder, or the like. Further, the compositions can be in a form suitable for use in transdermal devices. These formulations may be prepared, using a compound of formula (I), or a pharmaceutically acceptable salt thereof, via conventional processing methods. As an example, a cream or ointment is prepared by admixing hydrophilic material and water, together with about 5wt% to about 10wt% of the compound, to produce a cream or ointment having a desired consistency.

Pharmaceutical compositions of this invention can be in a form suitable for rectal administration wherein the carrier is a solid. It is preferable that the mixture forms unit dose suppositories. Suitable carriers include cocoa butter and other materials commonly used in the art. The suppositories may be conveniently formed by first admixing the composition with the softened or melted carrier(s) followed by chilling and shaping in molds.

In addition to the aforementioned carrier ingredients, the pharmaceutical formulations described above may include, as appropriate, one or more additional carrier ingredients such as diluents, buffers, flavoring agents, binders, surface-active agents, thickeners, lubricants, preservatives (including anti-oxidants) and the like. Furthermore, other adjuvants can be included to render the formulation isotonic with the blood of the intended recipient. Compositions containing a compound of formula (I), or pharmaceutically acceptable salts thereof, may also be prepared in powder or liquid concentrate form.

Generally, dosage levels on the order of 0.01mg/kg to about 150mg/kg of body weight per day are useful in the treatment of the above-indicated conditions, or alternatively about 0.5mg to about 7g per patient per day. For example, obesity may be effectively treated by the administration of from about 0.01 to 50mg of the compound per kilogram of body weight per day, or alternatively about 0.5mg to about 3.5g per patient per day.

It is understood, however, that the specific dose level for any particular patient will depend upon a variety of factors including the age, body weight, general health, sex, diet, time of administration, route of administration, rate of excretion, drug combination and the severity of the particular disease undergoing therapy.

The compounds of formula (I) may be used in the treatment of diseases or conditions in which GPRl 19 plays a role.

Thus the invention also provides a method for the treatment of a disease or condition in which GPRl 19 plays a role comprising a step of administering to a subject in need thereof an effective amount of a compound of formula (I), or a pharmaceutically acceptable salt thereof. Diseases or conditions in which GPRl 19 plays a role include obesity and diabetes. In the context of the present application the treatment of obesity is intended to encompass the treatment of diseases or conditions such as obesity and other eating disorders associated with excessive food intake e.g. by reduction of appetite and body weight, maintenance of weight reduction and prevention of rebound and diabetes (including Type 1 and Type 2 diabetes, impaired glucose tolerance, insulin resistance and diabetic complications such as neuropathy, nephropathy, retinopathy, cataracts, cardiovascular complications and dyslipidaemia). And the treatment of patients who have an abnormal sensitivity to ingested fats leading to functional dyspepsia. The compounds of the invention may also be used for treating metabolic diseases such as metabolic syndrome (syndrome X), impaired glucose tolerance, hyperlipidemia, hypertriglyceridemia, hypercholesterolemia, low HDL levels and hypertension.

The compounds of the invention may offer advantages over compounds acting via different mechanisms for the treatment of the above mentioned disorders in that they may offer beta-cell protection, increased cAMP and insulin secretion and also slow gastric emptying. The compounds of the invention may also be used for treating conditions characterised by low bone mass such asosteopenia, osteoporosis, rheumatoid arthritis, osteoarthritis, periodontal disease, alveolar bone loss, osteotomy bone loss, childhood idiopathic bone loss, Paget's disease, bone loss due to metastatic cancer, osteolytic lesions, curvature of the spine and loss of height.

The invention also provides a method for the regulation of satiety comprising a step of administering to a subject in need thereof an effective amount of a compound of formula (I), or a pharmaceutically acceptable salt thereof.

The invention also provides a method for the treatment of obesity comprising a step of administering to a subject in need thereof an effective amount of a compound of formula (I), or a pharmaceutically acceptable salt thereof.

The invention also provides a method for the treatment of diabetes, including Type 1 and Type 2 diabetes, particularly type 2 diabetes, comprising a step of administering to a patient in need thereof an effective amount of a compound of formula (I), or a pharmaceutically acceptable salt thereof.

The invention also provides a method for the treatment of metabolic syndrome (syndrome X), impaired glucose tolerance, hyper lipidemia, hypertriglyceridemia, hypercholesterolemia, low HDL levels or hypertension comprising a step of administering to a patient in need thereof an effective amount of a compound of formula (I), or a pharmaceutically acceptable salt thereof.

The invention also provides a compound of formula (I), or a pharmaceutically acceptable salt thereof, for use in the treatment of a condition as defined above.

The invention also provides the use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for the treatment of a condition as defined above.

In the methods of the invention the term "treatment" includes both therapeutic and prophylactic treatment.

The compounds of formula (I) may exhibit advantageous properties compared to known GPRl 19 agonists, for example, the compounds may exhibit improved solubility thus improving absorption properties and bioavailability, improved pharmacokinetic properties or other advantageous properties for compounds to be used as pharmaceuticals.

The compounds of formula (I), or pharmaceutically acceptable salts thereof, may be administered alone or in combination with one or more other therapeutically active compounds. The other therapeutically active compounds may be for the treatment of the same disease or condition as the compounds of formula (I) or a different disease or condition. The therapeutically active compounds may be administered simultaneously, sequentially or separately.

The compounds of formula (I) may be administered with other active compounds for the treatment of obesity and/or diabetes, for example insulin and insulin analogs, gastric lipase inhibitors, pancreatic lipase inhibitors, sulfonyl ureas and analogs, biguanides, α2 agonists, glitazones, PPAR-γ agonists, mixed PPAR-α/γ agonists, RXR agonists, fatty acid oxidation inhibitors, α-glucosidase inhibitors, dipeptidyl peptidase IV inhibitors, GLP-I agonists e.g. GLP-I analogues and mimetics, β-agonists, phosphodiesterase inhibitors, lipid lowering agents, glycogen phosphorylase inhibitors, antiobesity agents e.g. pancreatic lipase inhibitors, MCH-I antagonists and CB-I antagonists (or inverse agonists), amylin antagonists, lipoxygenase inhibitors, somostatin analogs, glucokinase activators, glucagon antagonists, insulin signalling agonists, PTPlB inhibitors, gluconeogenesis inhibitors, antilypolitic agents, GSK inhibitors, galanin receptor agonists, anorectic agents, CCK receptor agonists, leptin, serotonergic/dopaminergic antiobesity drugs, reuptake inhibitors e.g. sibutramine, CRF antagonists, CRF binding proteins, thyromimetic compounds, aldose reductase inhibitors, glucocorticoid receptor antagonists, NHE-I inhibitors or sorbitol dehydrogenase inhibitors.

Combination therapy comprising the administration of a compound of formula (I), or a pharmaceutically acceptable salt thereof, and at least one other antiobesity agent represents a further aspect of the invention.

The present invention also provides a method for the treatment of obesity in a mammal, such as a human, which method comprises administering an effective amount of a compound of formula (I), or a pharmaceutically acceptable salt thereof, and another antiobesity agent, to a mammal in need thereof.

The invention also provides the use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, and another antiobesity agent for the treatment of obesity.

The invention also provides the use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for use in combination with another antiobesity agent, for the treatment of obesity.

The compound of formula (I), or a pharmaceutically acceptable salt thereof, and the other antiobesity agent(s) may be co-administered or administered sequentially or separately.

Co-administration includes administration of a formulation which includes both the compound of formula (I), or a pharmaceutically acceptable salt thereof, and the other antiobesity agent(s), or the simultaneous or separate administration of different formulations of each agent. Where the pharmacological profiles of the compound of formula (I), or a pharmaceutically acceptable salt thereof, and the other antiobesity agent(s) allow it, coadministration of the two agents may be preferred.

The invention also provides the use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, and another antiobesity agent in the manufacture of a medicament for the treatment of obesity.

The invention also provides a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable salt thereof, and another antiobesity agent, and a pharmaceutically acceptable carrier. The invention also encompasses the use of such compositions in the methods described above.

GPRl 19 agonists are of particular use in combination with centrally acting antiobesity agents.

The other antiobesity agent for use in the combination therapies according to this aspect of the invention is preferably a CB-I modulator, e.g. a CB-I antagonist or inverse agonist. Examples of CB-I modulators include SR141716 (rimonabant) and SLV-319 ((45)-(-)-3-(4- chlorophenyl)-N-methyl-N- [(4-chlorophenyl)sulfonyl] -4-phenyl-4,5-dihydro- 1 H-pyrazole- 1 - carboxamide); as well as those compounds disclosed in EP576357, EP656354, WO 03/018060, WO 03/020217, WO 03/020314, WO 03/026647, WO 03/026648, WO 03/027076, WO 03/040105, WO 03/051850, WO 03/051851, WO 03/053431, WO 03/063781, WO 03/075660, WO 03/077847, WO 03/078413, WO 03/082190, WO 03/082191, WO 03/082833, WO 03/084930, WO 03/084943, WO 03/086288, WO 03/087037, WO 03/088968, WO 04/012671, WO 04/013120, WO 04/026301, WO 04/029204, WO 04/034968, WO 04/035566, WO 04/037823 WO 04/052864, WO 04/058145, WO 04/058255, WO 04/060870, WO 04/060888, WO 04/069837, WO 04/069837, WO 04/072076, WO 04/072077, WO 04/078261 and WO 04/108728, and the references disclosed therein.

Other diseases or conditions in which GPRl 19 has been suggested to play a role include those described in WO 00/50562 and US 6,468,756, for example cardiovascular disorders, hypertension, respiratory disorders, gestational abnormalities, gastrointestinal disorders, immune disorders, musculoskeletal disorders, depression, phobias, anxiety, mood disorders and Alzheimer's disease.

All publications, including, but not limited to, patents and patent application cited in this specification, are herein incorporated by reference as if each individual publication were specifically and individually indicated to be incorporated by reference herein as fully set forth.

The invention will now be described by reference to the following examples which are for illustrative purposes and are not to be construed as a limitation of the scope of the present invention.

EXAMPLES

Materials and methods

Column chromatography was carried out on SiO₂ (40-63 mesh) unless specified otherwise. LCMS data were obtained as follows: Atlantis 3// Ci₈ column (3.0 X 20.0 mm, flow rate = 0.85 mL/min) eluting with a H₂O-CH₃CN solution containing 0.1% HCO₂H over 6 min with UV detection at 220 nm. Gradient information: 0.0-0.3 min 100% H₂O; 0.3-4.25 min: Ramp up to 10% H₂O-90% CH₃CN; 4.25-4.4 min: Ramp up to 100% CH₃CN; 4.4-4.9 min: Hold at 100% CH₃CN; 4.9-6.0 min: Return to 100% H₂O. The mass spectra were obtained using an electrospray ionisation source in either the positive (ES⁺) or negative (ES^") ion modes.

Abbreviations and acronyms: Ac: Acetyl; t-Bu: tert-Butyl; DCM: Dichloromethane; DMP: Dess-Martin Periodinane; DIAD: Diisopropyl azodicarboxylate; DIPEA: N₅N- Diisopropylethylamine; EDCI: l-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride; Et: Ethyl; h: hour(s); min: minute/s; HATU: <9-(7-Azabenzotriazol-l-yl)-N,N,N,N- tetramethyluronium hexafluorophosphate; HOBt: 1-Hydroxybenzotriazole; IH: Isohexane; Me: Methyl; MeCN: Acetonitrile; ΝMP: Ν-methyl pyrollidine; Ph: Phenyl; RP-HPLC: Reverse phase-high performance liquid chromatography; RT: Retention time; TBAD: di-terZ-butyl azodicarboxylate; THF: Tetrahydrofuran; TMEDA: N₅N₅N₅N Tetramethylethylenediamine

The syntheses of the following compounds have been described elsewhere: 6-Chloro-2- methylnicotinic acid ethyl ester: WO06/095159; N-Hydroxyisobutyramidine: /. Org. Chem. 2003, 68, 7316-7321; 2-methyl-6-[l,2,4]triazol-l-yl-pyridin-3-ylamine: WO08/005569; 3- Piperidin-4-ylpropan-l-ol: Tetrahedron 1999, 55, 11619-11639; tert-Butyl 4-((£)-2- ethoxycarbonyl-l-methylvinyl)piperidine-l-carboxylate: US Patent 6,518,423. All other compounds were available from commercial sources.

Preparation 1: 2-Fluoro-5-methylsulfanylpyridine

To a solution of 5-bromo-2-fluoropyridine (3.00 g, 17.1 mmol) and TMEDA (3.35 mL, 22.2 mmol) in toluene (200 mL) at -75⁰C under argon was added 1.6 M «-butyllithium in hexane (12.8 mL, 20.5 mmol) over 10 min, and the mixture stirred for 50 min before dimethyldisulfide (1.84 mL, 20.5 mmol) was added. The reaction was stirred for 1 h at -75⁰C then warmed to 2⁰C and quenched with saturated NH₄Cl solution (40 mL). The organic phase was collected, washed with brine, dried (MgSO₄) and the solvent was removed under vacuum to give a residue which was purified by column chromatography (IH-EtOAc, 39:1) to furnish the title compound. RT = 2.64 min; mlz (ES⁺) = 143.95 [M + H]⁺.

Preparation 2: 2-Fluoro-5-methanesulfonylpyridine

To a solution of 2-fluoro-5-methylsulfanylpyridine (Preparation 1, 300 mg, 2.10 mmol) in DCM (7 mL) at O⁰C was added 77% 3-chloroperbenzoic acid (970 mg, 4.30 mmol) over 15 min. A further aliquot of DCM (5 mL) was added and the mixture stirred for 1 h. The reaction mixture was diluted with DCM (25 mL), washed with Na₂CO₃ (15 mL) and the organic phase was collected through a hydrophobic frit. The solvent was removed under vacuum to furnish the title compound. RT = 1.64 min; mlz (ES⁺) = 175.90 [M + H]⁺.

Preparation 3: 6-Fluoro-3-methanesulfonyl-2-methylpyridine

3-Bromo-6-fluoro-2-methylpyridine was converted into 6-fluoro-2-methyl-3- methylsulfanylpyridine employing a procedure similar to that outlined in Preparation 1: mlz (ES⁺) = 157.87 [M + H]⁺. Oxidation, utilising a protocol similar to that given in Preparation 2, furnished the title compound: RT = 2.13 min; mlz (ES⁺) = 189.89 [M + H]⁺.

Preparation 4: 4-(3-Hydroxypropyl)piperidine-l-carbonitrile

A slurry of NaHCO₃ (35.2 g, 0.42 mol) in H₂O (70 mL) was added to a stirred solution of 3-piperidin-4-ylpropan-l-ol (20.0 g, 0.14 mol) in DCM at 0⁰C. A solution of BrCN (17.8 g, 0.17 mol) in DCM (19 mL) was added to the reaction over 1 min, then stirring was continued at 0⁰C for 0.5 h. The reaction was then stirred at ambient temperature for 2 h, before being washed with saturated aqueous NaHCO₃ and brine. The DCM solution was dried (MgSO₄), filtered and concentrated in vacuo to furnish an oil that was dissolved in a small amount of DCM, before being filtered through a SiO₂ pad, eluting with EtOAc. The filtrate was concentrated under reduced pressure to afford the title compound: mlz (ES⁺) = 169.1 [M + H]⁺.

Preparation 5: 3-[l-(3-Isopropyl[l,2,4]oxadiazol-5-yl)piperidin-4-yl]propan-l-ol v-N

ΛH

_^JO

ZnCl₂ (IM in Et₂O, 145 mL, 145 mmol) was added over 20 min to a stirred solution of 4-(3-hydroxypropyl)piperidine-l-carbonitrile (Preparation 4, 20.3 g, 121 mmol) and N- hydroxyisobutyramidine (14.8 g, 145 mmol) in EtOAc (290 mL) and THF (270 mL). After 2 h, the white precipitate that had formed was collected and washed with THF-EtOAc (1:1, 50 mL). This precipitate was dissolved in EtOH (550 mL) and 12M HCl (70 mL), then the solution was stirred with heating to 70⁰C for 16 h. The EtOH was removed in vacuo, the remainder was diluted with H₂O, then the pH was adjusted to pH 7 with solid NaHCO₃. The mixture was extracted with EtOAc (3x), then the combined extracts were washed with brine, before being dried (MgSO₄). Filtration and solvent removal furnished the title compound: m/z (ES⁺) = 254.1 [M + H]⁺.

Preparation 6: tert-Butyl 4-((Zi)-2-carboxy-l -methyl vinyl)piperidine-l-carboxylate

A solution of terZ-butyl 4-((Zi)-2-ethoxycarbonyl-l -methyl vinyl)piperidine-l- carboxylate (18.7 g, 62.9 mmol) in MeOH (90 mL) and H₂O (25 mL) was treated with 2M NaOH (94.5 mL, 189 mmol). The reaction was stirred for 16 h, the MeOH was removed under reduced pressure, then the remainder was partitioned between EtOAc and H₂O. The aqueous layer was separated and acidified to pH 2 with 12M HCl, before being extracted with EtOAc (2x). The organic extracts were washed with brine, dried (MgSO₄), filtered, and concentrated in vacuo, then the remainder was recrystallised from EtOAc-IH to provide the title compound: m/z (ES^") = 268.3 [M- H]^".

Preparation 7: tert-Butyl 4-((R)-2-carboxy-l-methylethyl)piperidine-l-carboxylate

tert-Butyl 4-((Zi)-2-carboxy-l -methyl vinyl)piperidine-l-carboxylate (Preparation 6, 130 g, 0.483 mol) was placed in a hydrogenation flask under an Ar atmosphere, then degassed MeOH (400 mL) was added. [Rh(norbornadiene)₂]BF₄ (1.80 g, 4.81 mmol) and (S)-l-[(R)-2-(di- ter?-butylphosphino)ferrocenyl]ethylbis(2-methylphenyl)phosphine (2.90 g, 5.08 mmol) were placed in a separate Schlenk flask under Ar, before being treated with degassed MeOH (200 mL). This catalyst mixture was stirred for 15 min at ambient temperature, before being transferred via cannula into the hydrogenation flask. The Schlenk flask was rinsed with more degassed MeOH (100 mL). These washings were transferred to the hydrogenation flask, then more degassed MeOH (300 mL) was added. The hydrogenation flask was sealed, the Ar replaced by H₂, and the pressure set to 1.05 bar. The reaction mixture was heated to 35°C, and stirring/shaking was started. After 48 h, the reaction was stopped and a representative sample of the reaction mixture was analysed by HPLC and ¹H NMR. The conversion was 100% and the enantiomeric purity of the crude (R) -acid was 98.2%, as ascertained by the following HPLC method: Column: CHIRALPAK AD-H (previously used with CF₃CO₂H-containing solvents) 4.6 x 250 mm; Solvent: C₆Hi₄-ZPrOH (97:3 isocratic); Temperature: 20⁰C; Flow rate: 1 mL/min; UV -detection (210, 230 nm); Sample: 100 μL reaction solution dissolved with 1 inL MeOH. Retention times: (S)-acid: 19.3 min, (R)-acid: 20.6 min, starting enoic acid: 22.1 min. Isolation procedure: The MeOH was evaporated, then the crude hydrogenation product was dissolved in ?-BuOMe and extracted with aqueous NaOH. The aqueous phase was added to a mixture of IM HCl and EtOAc. The aqueous phase was extracted further with EtOAc, then the combined organic extracts were washed with brine and dried (MgSO₄). The title compound was isolated following filtration and complete removal of the solvent.

Preparation 8: terf-Butyl 4-((R)-3 -hydroxy- l-methylpropyl)piperidine-l-carboxylate

BH₃-THF (IM, 15.7 inL, 15.7 mmol) was added dropwise over 5 min to a stirred solution of tert-bvXy\ 4-((R)-2-carboxy-l-methylethyl)piperidine-l-carboxylate (Preparation 7, 1.70 g, 6.30 mmol) in anhydrous THF at 0⁰C. After 1 h, the reaction was treated with Et₂O, then with 2M HCl. The organic layer was washed with brine, before being dried (Na₂SO₄). Filtration, solvent evaporation, and column chromatography (EtOAc-CH₂Cl₂, 1:3) provided the title compound: RT = 3.17 min; mlz (ES⁺) = 258.1 [M + H]⁺.

Preparation 9: 4-((R)-3-Hydroxy- 1 -methylpropyl)piperidine- 1 -carbonitrile

A mixture of terZ-butyl 4-((R)-3-hydroxy-l-methylpropyl)piperidine-l-carboxylate (Preparation 8, 6.20 g, 14.9 mmol) and 4M HCl in dioxane (10 mL) were stirred at ambient temperature. After 3 h, the solvents were removed under reduced pressure to furnish the hydrochloride salt of (/?)-3-piperidin-4-yl-butan-l-ol: 4 ((CD₃J₂SO) 0.83 (d, 3H), 1.19-1.28 (m, IH), 1.38-1.59 (m, 5H), 1.64-1.76 (m, 2H), 2.75-2.87 (m, 2H), 3.20-3.30 (m, 2H), 3.35- 3.60 (m, 4H). A stirred mixture of this compound (930 mg, 4.8 mmol) and NaHCO₃ (1.61 g, 19.2 mmol) in DCM-H₂O (4:1, 15 mL) at 0⁰C was treated with a solution of BrCN (610 mg, 5.8 mmol) in DCM (2 mL). The reaction was stirred at 20⁰C for 2 h, before being partitioned between H₂O and DCM. The organic phase was separated and dried (MgSO₄). Filtration, solvent evaporation, and column chromatography (EtOAc) provided the title compound: RT = 2.45 min; mlz (ES⁺) = 183.1 [M + H]⁺.

Preparation 10: (/?)-3-[l-(3-Isopropyl-[l,2,4]oxadiazol-5-yl)piperidin-4-yl]butan-l-ol

Condensation of 4-((R)-3-hydroxy- 1 -methylpropyl)piperidine- 1 -carbonitrile (Preparation 9, 530 mg, 2.90 mmol) with N-hydroxyisobutyramidine (360 mg, 3.50 mmol), employing a procedure similar to that outlined in Preparation 5, afforded the title compound: RT = 2.92 min; mlz (ES⁺) = 268.1 [M + H]⁺.

Preparation 11: 2-{3-[l-(3-Isopropyl-[l,2,4]oxadiazol-5-yl)piperidin-4-yl]propyl}isoindole- 1,3-dione

DIAD (340 μL, 1.74 mmol) was added to a stirred solution of isoindole-l,3-dione (139 mg, 947 μmol), 3-[l-(3-isopropyl[l,2,4]oxadiazol-5-yl)piperidin-4-yl]propan-l-ol (Preparation 5, 200 mg, 789 μmol) and PPh₃ (435 mg, 1.66 mmol) in anhydrous THF (10 inL) at O⁰C. After stirring at ambient temperature for 1.5 h, the solvent was removed in vacuo, and the remainder was dissolved in EtOAc and washed with 2M NaOH (2x) and brine. The organic layer was dried (MgSO₄), concentrated under reduced pressure and the remainder was triturated with Et₂O. The solid produced was filtered and washed with Et₂O. The combined washings and filtrate were concentrated under reduced pressure and purified by column chromatography (Et₂O-IH; 2:3) to afford the title compound: RT = 3.82 min; mlz (ES⁺) = 382.99 [M + H]⁺.

Preparation 12: 3-[l -(3-Isopropyl-[l ,2,4]oxadiazol-5-yl)piperidin-4-yl]propylamine

Hydrazine (65% aqueous so ution, 123 μL, 1.65 mmol) was added to a stirred solution of 2- { 3 -[ 1 -(3 -isopropyl- [ 1 ,2,4] oxadiazol-5 -yl)piperidin-4-yl]propyl } isoindole- 1 ,3-dione (Preparation 11, 210 mg, 550 μmol) in MeOH (7 mL) and the resulting mixture was heated at 65⁰C for 16 h. The solvent was removed under vacuum and the remainder was dissolved in DCM and H₂O. The organic layer was extracted with 2 M HCl (2x) and the combined acidic extracts were basified to pH 10 with 2M NaOH, before being extracted with DCM (2x). The combined organic extracts were dried (MgSO₄), filtered and concentrated under reduced pressure to afford the title compound: RT = 2.17 min; mlz (ES⁺) = 253.06 [M + H]⁺.

Preparation 13 : 2- { (/?)-3 -[ 1 -(3-Isopropyl- [ 1 ,2,4] oxadiazol-5 -yl)piperidin-4-yl]butyl } - isoindole- 1 ,3-dione

TBAD (400 mg, 1.74 mmol) was added to a stirred solution of isoindole-l,3-dione (139 mg, 947 μmol), 3-[l-(3-isopropyl[l,2,4]oxadiazol-5-yl)piperidin-4-yl]propan-l-ol (Preparation 10, 200 mg, 748 μmol) and PPh₃ (435 mg, 1.66 mmol) in anhydrous THF (10 mL) at O⁰C. After stirring at ambient temperature for 1.5 h, the solvent was removed in vacuo, then the remainder was dissolved in EtOAc and washed with 2M NaOH (2x) and brine. The organic layer was dried (MgSO₄), concentrated under reduced pressure and the remainder was triturated with Et₂O. The solid produced was filtered and washed with Et₂O. The combined washings and filtrate were concentrated under reduced pressure and purified by column chromatography (Et₂O-IH; 1:1) to afford the title compound: RT = 4.02 min; mlz (ES⁺) = 397.01 [M + H]⁺.

Preparation 14: (R)-3-[l-(3-Isopropyl-[l,2,4]oxadiazol-5-yl)piperidin-4-yl]butylamine

This compound was prepared from 2-{(R)-3-[l-(3-isopropyl-[l,2,4]oxadiazol-5-yl)- piperidin-4-yl]butyl}isoindole-l,3-dione (Preparation 13, 257 mg, 650 μmol) using a procedure similar to that outlined in Preparation 12; RT = 2.36 min; mlz (ES⁺) = 267.05 [M + H]⁺.

Preparation 15: 6-{3-[l-(3-Isopropyl-[l,2,4]oxadiazol-5-yl)piperidin-4-yl]propylamino}-2- methylnicotinic acid ethyl ester

3-[l-(3-Isopropyl-[l,2,4]oxadiazol-5-yl)piperidin-4-yl]propylamine (Preparation 12, 160 mg, 630 μmol) was added to a stirred solution of 6-chloro-2-methylnicotinic acid ethyl ester and DIPEA (165 μL, 950 μmol) in THF (5 mL). The resulting solution was stirred at 6O⁰C for 16 h, followed by heating in the microwave at 95⁰C for 1 h. Further 6-chloro-2-methylnicotinic acid ethyl ester (633mg, 3.15 mmol) was added to the reaction mixture and the resulting solution was heated in the microwave at 100⁰C for 2 h, followed by removal of the solvent in vacuo. The remainder was diluted with EtOAc and H₂O, then the organic layer was washed with brine, dried (MgSO₄), concentrated in vacuo and purified by column chromatography (EtOAc-IH; 1:4) to provide the title compound: RT = 2.97 min; mlz (ES⁺) = 416.26 [M + H]⁺. Preparation 16: 6- { (/?)-3-[ 1 -(3-Isopropyl-[ 1 ,2,4]oxadiazol-5-yl)piperidin-4-yl]butylamino } -2- methylnicotinic acid

6-Fluoro-2-methylnicotinic acid (400 mg, 2.58 mmol) and DIPEA (900 μL, 5.16 mmol) were added to a solution of (R)-3-[l-(3-isopropyl-[l,2,4]oxadiazol-5-yl)piperidin-4-yl]- butylamine (Preparation 14, 690 mg, 2.58 mmol) in NMP (3 mL) and the resulting solution heated in the microwave at 100⁰C for 7 h. The reaction mixture was diluted with EtOAc, washed with IM HCl and the acidic washings basified to pH 5 with IM NaOH, before being extracted with EtOAc. The organic extracts were washed with H₂O, brine, dried (MgSO₄), filtered and concentrated in vacuo. Purification by column chromatography (EtOAc-IH-AcOH; 69:30:1) afforded the title compound: RT = 2.70 min; mlz (ES⁺) = 402.69 [M + H]⁺.

Preparation 17: 6- { (R)-3-[ 1 -(3-Isopropyl-[ 1 ,2,4]oxadiazol-5-yl)piperidin-4-yl]butylamino } -2- methylnicotinic acid hydrazide

HOBt (173 mg, 1.28 mmol) and EDCI (245 mg, 1.28 mmol) were added to a suspension of 6-{(R)-3-[l-(3-isopropyl-[l,2,4]oxadiazol-5-yl)piperidin-4-yl]butylamino}-2-methyl- nicotinic acid (Preparation 16, 430 mg, 1.07 mmol) in MeCN (2.3 mL) and the resulting suspension stirred at ambient temperature for 2.5 h, before being slowly added to a solution of hydrazine hydrate (107 mg, 2.14 mmol) in MeCN (1.7 mL) at O⁰C. The reaction mixture was stirred at O⁰C for 1 h, before quenching with H₂O and extracting with EtOAc. The organic extracts were washed with saturated aqueous NaHCO₃ solution, dried (MgSO₄), filtered and concentrated in vacuo to afford the title compound: RT = 2.48 min; mlz (ES⁺) = 416.31 [M + H]⁺.

Preparation 18: 6-Methyl-5-[l,2,4]triazol-l-ylpyridin-2-ylamine

5-Iodo-6-methylpyridin-2-ylamine (2.00 g, 8.55 mmol), IH-[1, 2,4] triazole (590 mg, 8.55 mmol), CuI (80.0 mg, 430 μmol), K₃PO₄ (3.80 g, 18.0 mmol) and N,N-dimethylethane- 1,2-diamine (190 μL, 1.71 mmol) in DMF (20 mL) were heated in the microwave at 15O⁰C for 9 h. The reaction mixture was cooled to ambient temperature and diluted with H₂O and EtOAc. The aqueous layer was extracted with EtOAc (6x) and the combined organic extracts were washed with brine, dried (MgSO₄), filtered and concentrated in vacuo. Purification by column chromatography (EtOAc-IH; 1:9) afforded the title compound: RT = 0.26 min; mlz (ES⁺) = 175.97 [M + H]⁺.

Preparation 19: (R)-3-[l-(3-Isopropyl-[l,2,4]oxadiazol-5-yl)piperidin-4-yl]butyraldehyde

A solution of (R)-3-[l-(3-isopropyl-[l,2,4]oxadiazol-5-yl)piperidin-4-yl]butan-l-ol (Preparation 10, 1.75 g, 6.55 mmol) in DCM (48 mL) was added over 10 min to a solution of DMP (3.06 g, 7.21 mmol) in DCM (72 mL) at O⁰C. The resulting reaction mixture was stirred at ambient temperature for 2 h before removing the solvent in vacuo. The residue was dissolved in Et₂O, washed repeatedly with 2M NaOH, dried (MgSO₄), filtered and concentrated in vacuo. Purification by column chromatography (EtOAc-IH; 3:7 to 2:3) afforded the title compound: RT = 3.30 min; mlz (ES⁺) = 266.16 [M + H]⁺.

Example 1 : { (R)-3-[ 1 -(3-Isopropyl-[ 1 ,2,4]oxadiazol-5-yl)piperidin-4-yl]butyl } -(5- methanesulfonylpyridin-2-yl)amine

2-Fluoro-5-methanesulfonylpyridine (Preparation 2, 17 mg, 95.0 μmol) was added to a solution of (R)-3-[l-(3-isopropyl-[l,2,4]oxadiazol-5-yl)piperidin-4-yl]butylamine (Preparation 14, 21.0 mg, 78.9 μmol) and DIPEA (14.0 μL, 78.9 μmol) in THF (1 mL) and the reaction mixture was heated at 6O⁰C for 24 h. The THF was removed in vacuo and the remainder was purified by column chromatography (EtOAc-IH, 1:1 to 4:1) to afford the title compound: Sn (CDCl₃) 0.99 (d, 3H), 1.32 (d, 6H), 1.34-1.61 (m, 5H), 1.68-1.84 (m, 3H), 2.92 (sept, IH), 2.97- 3.08 (m, 5H), 3.32-3.56 (m, 2H), 4.18-4.27 (m, 2H), 5.07 (s, br, IH), 6.44 (d, IH), 7.85-7.89 (m, IH), 8.62-8.65 (m, IH); RT = 3.34 min; mlz (ES⁺) = 422.00 [M + H]⁺.

The compounds listed in Table 1 were synthesised by condensing 6-fluoro-3- methanesulfonyl-2-methylpyridine (Preparation 3) with the appropriate amine (Preparation 12 or Preparation 14), employing a procedure similar to that outlined in Example 1.

Table 1

Example 4: N-((R)-2-Hydroxy-l-methylethyl)-6-{3-[l-(3-isopropyl-[l,2,4]oxadiazol-5-yl)- piperidin-4-yl]propylamino } -2-methylnicotinamide

LiOH-H₂O (28.6 mg, μmol) was added to a solution of 6-{3-[l-(3-isopropyl- [l,2,4]oxadiazol-5-yl)piperidin-4-yl]propylamino}-2-methylnicotinic acid ethyl ester (Preparation 15, 126 mg, 300 μmol) in MeOH (10 mL) and H₂O (1 mL) and the resulting solution was heated at 5O⁰C for 120 h. The MeOH was evaporated off under reduced pressure, then the remainder was partitioned between H₂O and Et₂O. The aqueous phase was concentrated in vacuo, azeotroping with MeOH (2x) to furnish 6-{3-[l-(3-isopropyl-[l,2,4]oxadiazol-5-yl)- piperidin-4-yl]propylamino}-2-methylnicotinic acid anion. This compound was added to a solution of (tf)-2-aminopropan-l-ol (27.0 μL, 340 μmol), HATU (155 mg, 410 μmol) and DIPEA (237 μL, 1.36 mmol) in THF (5 mL) and the resulting solution was stirred at ambient temperature for 16 h. The reaction mixture was diluted with DCM (50 mL), washed with H₂O, saturated aqueous NaHCO₃ solution and brine, dried (MgSO₄), concentrated in vacuo and purified by RP-HPLC to provide the title compound: RT = 2.48 min; mlz (ES⁺) = 445.35 [M + H]⁺.

Example 5: {3-[l-(3-Isopropyl-[l,2,4]oxadiazol-5-yl)piperidin-4-yl]propyl}-(6-methyl-5- [l,3,4]oxadiazol-2-ylpyridin-2-yl)amine

A solution of 6-{(R)-3-[l-(3-isopropyl-[l,2,4]oxadiazol-5-yl)piperidin-4-yl]- butylamino}-2-methylnicotinic acid hydrazide (Preparation 17, 292 mg, 703 μmol) in trimethylorthoformate (8 mL) was heated in the microwave at 12O⁰C for 15 min. The solvent was removed in vacuo and the residue dissolved in EtOAc and washed with saturated aqueous NaHCO₃ solution, H₂O and brine, dried (MgSO₄), filtered and concentrated in vacuo. Purification by column chromatography (EtOAc-IH, 1:1) afforded the title compound: RT = 2.07 min; mlz (ES⁺) = 426.29 [M + H]⁺.

Example 6 : { (R)-3- [ 1 -(3-Isopropyl- [ 1 ,2,4] oxadiazol-5 -yl)piperidin-4-yl]butyl } -(2-methyl-6- [l,2,4]triazol-l-ylpyridin-3-yl)amine

2-Methyl-6-[l,2,4]triazol-l-ylpyridin-3-ylamine (100 mg, 570 μmol) was added to a solution of (R)-3-[l-(3-isopropyl-[l,2,4]oxadiazol-5-yl)piperidin-4-yl]butyraldehyde (Preparation 19, 151 mg, 570 μmol) in EtOH (10 mL) and the resulting solution heated at 78⁰C for 3.5 h. The reaction mixture was cooled to O⁰C and NaBH₄ (22.0 mg, 570 μmol) was added before warming the reaction mixture to ambient temperature and stirring for 16 h. The solvent was removed in vacuo and the resulting residue was dissolved in EtOAc, washed with IM HCl, 2M NaOH and brine, dried (MgSO₄), filtered and concentrated in vacuo. Purification by RP- HPLC afforded the title compound: RT = 2.52 min; mlz (ES⁺) = 425.63 [M + H]⁺.

Example 7: { (R)-3- [ 1 -(3-Isopropyl- [ 1 ,2,4] oxadiazol-5 -yl)piperidin-4-yl]butyl } -(6-methyl-5 - [l,2,4]triazol-l-ylpyridin-2-yl)amine

6-Methyl-5-[l,2,4]triazol-l-ylpyridin-2-ylamine (Preparation 18, 115 mg, 660 μmol) was added to a solution of (/?)-3-[l-(3-isopropyl-[l,2,4]oxadiazol-5-yl)piperidin-4-yl]- butyraldehyde (Preparation 19, 263 mg, 990 μmol) in EtOH (10 mL) and the resulting solution heated at 78⁰C for 16 h. The reaction mixture was cooled to O⁰C and NaBH₄ (25.0 mg, 660 μmol) was added before warming the reaction mixture to ambient temperature and stirring for 16 h. The solvent was removed in vacuo and the resulting residue dissolved in EtOAc and extracted with IM HCl. The acidic extracts were basified to pH 7 with IM NaOH and extracted with EtOAc. The organic extracts were dried (MgSO₄), filtered, concentrated in vacuo and purified by column chromatography (EtOAc-MeOH, 19:1) to afford the title compound: RT = 2.79 min; mlz (ES⁺) = 425.19 [M + H]⁺.

The biological activity of the compounds of the invention may be tested in the following assay systems:

Yeast Reporter Assay

The yeast cell-based reporter assays have previously been described in the literature (e.g. see Miret J. J. et al, 2002, J. Biol. Chem., 277:6881-6887; Campbell R.M. et al, 1999, Bioorg. Med. Chem. Lett., 9:2413-2418; King K. et al, 1990, Science, 250:121-123); WO 99/14344; WO 00/12704; and US 6,100,042). Briefly, yeast cells have been engineered such that the endogenous yeast G-alpha (GPAl) has been deleted and replaced with G-protein chimeras constructed using multiple techniques. Additionally, the endogenous yeast GPCR, Ste3 has been deleted to allow for heterologous expression of a mammalian GPCR of choice. In the yeast, elements of the pheromone signaling transduction pathway, which are conserved in eukaryotic cells (for example, the mitogen-activated protein kinase pathway), drive the expression of Fusl. By placing β-galactosidase (LacZ) under the control of the Fusl promoter (Fuslp), a system has been developed whereby receptor activation leads to an enzymatic readout.

Yeast cells were transformed by an adaptation of the lithium acetate method described by Agatep et al, (Agatep, R. et al, 1998, Transformation of Saccharomyces cerevisiae by the lithium acetate/single-stranded carrier DNA/polyethylene glycol (LiAc/ss-DNA/PEG) protocol. Technical Tips Online, Trends Journals, Elsevier). Briefly, yeast cells were grown overnight on yeast tryptone plates (YT). Carrier single-stranded DNA (lOμg), 2μg of each of two Fuslp- LacZ reporter plasmids (one with URA selection marker and one with TRP), 2μg of GPRl 19 (human or mouse receptor) in yeast expression vector (2μg origin of replication) and a lithium acetate/ polyethylene glycol/ TE buffer was pipetted into an Eppendorf tube. The yeast expression plasmid containing the receptor/ no receptor control has a LEU marker. Yeast cells were inoculated into this mixture and the reaction proceeds at 30⁰C for 60min. The yeast cells were then heat-shocked at 42°C for 15min. The cells were then washed and spread on selection plates. The selection plates are synthetic defined yeast media minus LEU, URA and TRP (SD- LUT). After incubating at 30⁰C for 2-3 days, colonies that grow on the selection plates were then tested in the LacZ assay.

In order to perform fluorimetric enzyme assays for β-galactosidase, yeast cells carrying the human or mouse GPRl 19 receptor were grown overnight in liquid SD-LUT medium to an unsaturated concentration (i.e. the cells were still dividing and had not yet reached stationary phase). They were diluted in fresh medium to an optimal assay concentration and 90μl of yeast cells added to 96-well black polystyrene plates (Costar). Compounds, dissolved in DMSO and diluted in a 10% DMSO solution to 1OX concentration, were added to the plates and the plates placed at 30⁰C for 4h. After 4h, the substrate for the β-galactosidase was added to each well. In these experiments, Fluorescein di (β-D-galactopyranoside) was used (FDG), a substrate for the enzyme that releases fluorescein, allowing a fluorimetric read-out. 20μl per well of 500μM FDG/2.5% Triton XlOO was added (the detergent was necessary to render the cells permeable). After incubation of the cells with the substrate for 60min, 20μl per well of IM sodium carbonate was added to terminate the reaction and enhance the fluorescent signal. The plates were then read in a fluorimeter at 485/535nm.

The compounds of the invention give an increase in fluorescent signal of at least ~ 1.5- fold that of the background signal (i.e. the signal obtained in the presence of 1% DMSO without compound). Compounds of the invention which give an increase of at least 5 -fold may be preferred.

cAMP Assay

A stable cell line expressing recombinant human GPRl 19 was established and this cell line may be used to investigate the effect of compounds of the invention on intracellular levels of cyclic AMP (cAMP). The cell monolayers are washed with phosphate buffered saline and stimulated at 37°C for 30min with various concentrations of compound in stimulation buffer plus 1 % DMSO. Cells are then lysed and cAMP content determined using the Perkin Elmer AlphaScreen™ (Amplified Luminescent Proximity Homogeneous Assay) cAMP kit. Buffers and assay conditions are as described in the manufacturer's protocol.

In vivo feeding study

The effect of compounds of the invention on body weight and food and water intake may be examined in freely-feeding male Sprague-Dawley rats maintained on reverse-phase lighting. Test compounds and reference compounds are dosed by appropriate routes of administration (e.g. intraperitoneally or orally) and measurements made over the following 24 h. Rats are individually housed in polypropylene cages with metal grid floors at a temperature of 21±4°C and 55+20% humidity. Polypropylene trays with cage pads are placed beneath each cage to detect any food spillage. Animals are maintained on a reverse phase light-dark cycle (lights off for 8 h from 09.30-17.30 h) during which time the room was illuminated by red light. Animals have free access to a standard powdered rat diet and tap water during a two week acclimatization period. The diet is contained in glass feeding jars with aluminum lids. Each lid had a 3-4 cm hole in it to allow access to the food. Animals, feeding jars and water bottles are weighed (to the nearest 0.1 g) at the onset of the dark period. The feeding jars and water bottles are subsequently measured 1 , 2, 4, 6 and 24 h after animals are dosed with a compound of the invention and any significant differences between the treatment groups at baseline compared to vehicle -treated controls.

Anti-diabetic effects of compounds of the invention in an in-vitro model of pancreatic beta cells (HIT-T15)

Cell Culture

HIT-T15 cells (passage 60) were obtained from ATCC, and were cultured in RPMI1640 medium supplemented with 10% fetal calf serum and 3OnM sodium selenite. All experiments were done with cells at less than passage 70, in accordance with the literature, which describes altered properties of this cell line at passage numbers above 81 (Zhang HJ, Walseth TF, Robertson RP. Insulin secretion and cAMP metabolism in HIT cells. Reciprocal and serial passage -dependent relationships. Diabetes. 1989 Jan;38(l):44-8).

cAMP assay

HIT-T 15 cells were plated in standard culture medium in 96-well plates at 100,000 cells/ 0.1ml/ well and cultured for 24 hr and the medium was then discarded. Cells were incubated for 15min at room temperature with lOOμl stimulation buffer (Hanks buffered salt solution, 5mM HEPES, 0.5mM IBMX, 0.1% BSA, pH 7.4). This was discarded and replaced with compound dilutions over the range 0.001, 0.003, 0.01, 0.03, 0.1, 0.3, 1, 3, 10, 30 μM in stimulation buffer in the presence of 0.5% DMSO. Cells were incubated at room temperature for 30min. Then 75ul lysis buffer (5mM HEPES, 0.3% Tween-20, 0.1% BSA, pH 7.4) was added per well and the plate was shaken at 900 rpm for 20 min. Particulate matter was removed by centrifugation at 3000rpm for 5min, then the samples were transferred in duplicate to 384-well plates, and processed following the Perkin Elmer AlphaScreen cAMP assay kit instructions. Briefly 25 μl reactions were set up containing 8μl sample, 5μl acceptor bead mix and 12μl detection mix, such that the concentration of the final reaction components is the same as stated in the kit instructions. Reactions were incubated at room temperature for 150min, and the plate was read using a Packard Fusion instrument. Measurements for cAMP were compared to a standard curve of known cAMP amounts (0.01, 0.03, 0.1, 0.3, 1, 3, 10, 30, 100, 300, 1000 nM) to convert the readings to absolute cAMP amounts. Data was analysed using XLfit 3 software.

Representative compounds of the invention were found to increase cAMP at an EC₅₀ of less than 10 μM. Compounds showing an EC₅₀ of less than 1 μM in the cAMP assay may be preferred.

Insulin secretion assay

HIT-T15 cells are plated in standard culture medium in 12-well plates at 106 cells/ 1 ml/ well and cultured for 3 days and the medium then discarded. Cells are washed x 2 with supplemented Krebs-Ringer buffer (KRB) containing 119 mM NaCl, 4.74 mM KCl, 2.54 mM CaCl₂, 1.19 mM MgSO₄, 1.19 mM KH2PO4, 25 mM NaHCO₃, 1OmM HEPES at pH 7.4 and 0.1% bovine serum albumin. Cells are incubated with 1ml KRB at 37°C for 30 min which is then discarded. This is followed by a second incubation with KRB for 30 min, which is collected and used to measure basal insulin secretion levels for each well. Compound dilutions (0, 0.1, 0.3, 1, 3, 10 uM) are then added to duplicate wells in 1ml KRB, supplemented with 5.6 mM glucose. After 30 min incubation at 37°C samples are removed for determination of insulin levels. Measurement of insulin is done using the Mercodia Rat insulin ELISA kit, following the manufacturers instructions, with a standard curve of known insulin concentrations. For each well insulin levels are corrected by subtraction of the basal secretion level from the preincubation in the absence of glucose. Data was analysed using XLfit 3 software.

Oral Glucose Tolerance Tests

The effects of compounds of the invention on oral glucose (GIc) tolerance were evaluated in male Sprague-Dawley rats. Food was withdrawn 16 h before administration of GIc and remained withdrawn throughout the study. Rats had free access to water during the study. A cut was made to the animals' tails, then blood (1 drop) was removed for measurement of basal GIc levels 60 min before administration of the GIc load. Then, the rats were weighed and dosed orally with test compound or vehicle (20% aqueous hydroxypropyl-^-cyclodextrin) 45 min before the removal of an additional blood sample and treatment with the GIc load (2 g kg^"1 p.o.). Blood samples were then taken from the cut tip of the tail 5, 15, 30, 60, 120, and 180 min after GIc administration. Blood glucose levels were measured just after collection using a commercially available glucose-meter (OneTouch® UltraTM from Lifescan). Representative compounds of the invention statistically reduced the GIc excursion at doses of <10 mg kg^"1. The effects of compounds of the invention on oral glucose (GIc) tolerance may also evaluated in male C57B1/6 or male oblob mice. Food is withdrawn 5 h before administration of GIc and remained withdrawn throughout the study. Mice have free access to water during the study. A cut is made to the animals' tails, then blood (20 μL) is removed for measurement of basal GIc levels 45 min before administration of the GIc load. Then, the mice are weighed and dosed orally with test compound or vehicle (20% aqueous hydroxypropyl-^-cyclodextrin or 25% aqueous Gelucire 44/14) 30 min before the removal of an additional blood sample (20 μL) and treatment with the GIc load (2-5 g kg^"1 p.o.). Blood samples (20 μL) are then taken 25, 50, 80, 120, and 180 min after GIc administration. The 20 μL blood samples for measurement of GIc levels are taken from the cut tip of the tail into disposable micro-pipettes (Dade Diagnostics Inc., Puerto Rico) and the sample added to 480 μL of haemolysis reagent. Duplicate 20 μL aliquots of the diluted haemolysed blood are then added to 180 μL of Trinders glucose reagent (Sigma enzymatic (Trinder) colorimetric method) in a 96-well assay plate. After mixing, the samples are left at rt for 30 min before being read against GIc standards (Sigma glucose/urea nitrogen combined standard set).

Claims

WHAT IS CLAIMED IS:

1. A compound of formula (I), or a pharmaceutically acceptable salt thereof:

(I) wherein one of X and Y is O and the other is N; one of E and Q is N and the other is CR²; R¹ is -SO₂R⁵, -CONHR⁶, oxadiazolyl or triazolyl; R² is hydrogen or methyl; R³ is hydrogen or methyl; R⁴ is C_2-5alkyl; R⁵ is Ci_₃alkyl; and R⁶ is hydrogen, Ci_₃alkyl, or C₂_₃alkyl substituted by one or two hydroxy groups.

2. A compound according to claim 1 , or a pharmaceutically acceptable salt thereof, wherein X is O.

3. A compound according to claim 1, or a pharmaceutically acceptable salt thereof, wherein Y is O.

4. A compound according to any one of claims 1 to 3, or a pharmaceutically acceptable salt thereof, wherein Q is N and E is CR².

5. A compound according to any one of claims 1 to 4, or a pharmaceutically acceptable salt thereof, wherein R¹ is -SO₂R⁵.

6. A compound according to claim 5, or a pharmaceutically acceptable salt thereof, wherein R¹ is -SO₂CH₃.

7. A compound according to any one of claims 1 to 4, or a pharmaceutically acceptable salt thereof, wherein R¹ is -CONHR⁶.

8. A compound according to claim 7, or a pharmaceutically acceptable salt thereof, wherein R⁶ is Ci_₃alkyl or C₂_₃alkyl substituted by one or two hydroxy groups.

9. A compound according to any one of claims 1 to 8, or a pharmaceutically acceptable salt thereof, wherein R³ is hydrogen.

10. A compound according to any one of claims 1 to 8, or a pharmaceutically acceptable salt thereof, wherein R³ is methyl.

11. A compound according to claim 10, or a pharmaceutically acceptable salt thereof, wherein R³ is methyl and the stereocentre produced has the (/^-configuration.

12. A compound according to any one of claims 1 to 11, or a pharmaceutically acceptable salt thereof, wherein R² is methyl.

13. A compound according to any one of claims 1 to 12, or a pharmaceutically acceptable salt thereof, wherein R⁴ is C₂-_Λ alkyl.

14. A compound according to claim 13, or a pharmaceutically acceptable salt thereof, wherein R⁴ is isopropyl.

15. A compound of formula (I) as defined in any one of Examples 1 to 7, or a pharmaceutically acceptable salt thereof.

16. A pharmaceutical composition comprising a compound according to any one of claims 1 to 15, or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier.

17. A method for the treatment of a disease or condition in which GPRl 19 plays a role comprising a step of administering to a subject in need thereof an effective amount of a compound according to any one of claims 1 to 15, or a pharmaceutically acceptable salt thereof.

18. A method for the regulation of satiety comprising a step of administering to a subject in need thereof an effective amount of a compound according to any one of claims 1 to 15, or a pharmaceutically acceptable salt thereof.

19. A method for the treatment of obesity comprising a step of administering to a subject in need thereof an effective amount of a compound according to any one of claims 1 to 15, or a pharmaceutically acceptable salt thereof.

20. A method for the treatment of diabetes comprising a step of administering to a subject in need thereof an effective amount of a compound according to any one of claims 1 to 15, or a pharmaceutically acceptable salt thereof.

21. A method for the treatment of metabolic syndrome (syndrome X), impaired glucose tolerance, hyperlipidemia, hypertriglyceridemia, hypercholesterolemia, low HDL levels or hypertension comprising a step of administering to a patient in need thereof an effective amount of a compound according to any one of claims 1 to 15, or a pharmaceutically acceptable salt thereof.

22. A compound according to any one of claims 1 to 15, or a pharmaceutically acceptable salt thereof, for use as a medicament.

23. The use of a compound according to any one of claims 1 to 15, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for the treatment or prevention of a disease or condition as defined in any one of claims 17 to 21.

24. A compound according to any one of claims 1 to 15 or a pharmaceutically acceptable salt thereof, for use in the treatment or prevention of a disease or condition as defined in any one of claims 17 to 21.