WO2010003153A3 - Analyse de la méthylation de paires conjuguées - Google Patents

Analyse de la méthylation de paires conjuguées Download PDF

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Publication number
WO2010003153A3
WO2010003153A3 PCT/US2009/049724 US2009049724W WO2010003153A3 WO 2010003153 A3 WO2010003153 A3 WO 2010003153A3 US 2009049724 W US2009049724 W US 2009049724W WO 2010003153 A3 WO2010003153 A3 WO 2010003153A3
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WIPO (PCT)
Prior art keywords
mate
methylation
methods
pair
tag sequence
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PCT/US2009/049724
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English (en)
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WO2010003153A2 (fr
Inventor
Kevin J. Mckernan
Benjamin G. Schroeder
Victoria L. Boyd
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Life Technologies Corporation
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Publication date
Application filed by Life Technologies Corporation filed Critical Life Technologies Corporation
Publication of WO2010003153A2 publication Critical patent/WO2010003153A2/fr
Publication of WO2010003153A3 publication Critical patent/WO2010003153A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention concerne des méthodes d’analyse de la méthylation d’acides nucléiques. Parmi les méthodes figurent celles qui conduisent à la préparation de bibliothèques de paires conjuguées appropriées pour un séquençage d’ADN fortement multiplexe. Les modes de réalisation comprennent des méthodes de préparation de bibliothèques de paires conjuguées contenant une première séquence indicatrice et une seconde séquence indicatrice, l’une des deux séquences indicatrices ayant été convertie par un agent de conversion de méthylation et l’autre séquence indicatrice n’ayant pas été convertie par l’agent de conversion de méthylation. D’autres modes de réalisation concernent des intermédiaires permettant de fabriquer la bibliothèque de paires conjuguées et des kits de fabrication des bibliothèques de paires conjuguées. L’invention concerne également un logiciel et des systèmes informatiques permettant d’analyser les taux de méthylation de l’ADN génomique d’où proviennent les séquences indicatrices.
PCT/US2009/049724 2008-07-03 2009-07-06 Analyse de la méthylation de paires conjuguées WO2010003153A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US13389108P 2008-07-03 2008-07-03
US61/133,891 2008-07-03
US14997609P 2009-02-04 2009-02-04
US61/149,976 2009-02-04

Publications (2)

Publication Number Publication Date
WO2010003153A2 WO2010003153A2 (fr) 2010-01-07
WO2010003153A3 true WO2010003153A3 (fr) 2010-05-14

Family

ID=41466622

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/049724 WO2010003153A2 (fr) 2008-07-03 2009-07-06 Analyse de la méthylation de paires conjuguées

Country Status (2)

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US (1) US20100120034A1 (fr)
WO (1) WO2010003153A2 (fr)

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US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
EP2753714B1 (fr) 2011-09-06 2017-04-12 Gen-Probe Incorporated Gabarits mis sous forme circulaire pour séquençage
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US9404147B2 (en) 2011-12-19 2016-08-02 Gen-Probe Incorporated Closed nucleic acid structures
EP2807292B1 (fr) 2012-01-26 2019-05-22 Tecan Genomics, Inc. Compositions et procédés pour l'enrichissement en séquence d'acide nucléique ciblée et la génération d'une banque à efficacité élevée
US9238836B2 (en) 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
WO2013163207A1 (fr) 2012-04-24 2013-10-31 Pacific Biosciences Of California, Inc. Identification d'une modification de type 5-méthyl-c dans des matrices d'acides nucléiques
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US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
US20150021496A1 (en) * 2013-07-17 2015-01-22 Muhammad Imran Shabbir Method to assist gel analysis and processing and apparatus for the same
EP3068883B1 (fr) 2013-11-13 2020-04-29 Nugen Technologies, Inc. Compositions et procédés pour l'identification d'une lecture de séquençage en double
CN103555856B (zh) * 2013-11-15 2015-02-11 复旦大学 一种全基因组dna甲基化的导向测序技术
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
US10102337B2 (en) 2014-08-06 2018-10-16 Nugen Technologies, Inc. Digital measurements from targeted sequencing
EP3207169B1 (fr) * 2014-10-14 2020-07-01 MGI Tech Co., Ltd. Construction de banques "mate pair"
EP4220645A3 (fr) 2015-05-14 2023-11-08 Life Technologies Corporation Séquences de code-barre, et systèmes et procédés associés
US10619205B2 (en) 2016-05-06 2020-04-14 Life Technologies Corporation Combinatorial barcode sequences, and related systems and methods
US20190119746A1 (en) * 2017-10-20 2019-04-25 Nugen Technologies, Inc. Oligonucleotides for selective amplification of nucleic acids
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN110205683B (zh) * 2019-06-25 2022-12-20 广州燃石医学检验所有限公司 Dna文库的制备方法和对dna文库的分析方法
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WO2010003153A2 (fr) 2010-01-07
US20100120034A1 (en) 2010-05-13

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