WO2010000854A3 - New bacteriophage adhesion proteins - Google Patents

New bacteriophage adhesion proteins Download PDF

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Publication number
WO2010000854A3
WO2010000854A3 PCT/EP2009/058453 EP2009058453W WO2010000854A3 WO 2010000854 A3 WO2010000854 A3 WO 2010000854A3 EP 2009058453 W EP2009058453 W EP 2009058453W WO 2010000854 A3 WO2010000854 A3 WO 2010000854A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
adhesion proteins
bacteriophage adhesion
proteins
relates
Prior art date
Application number
PCT/EP2009/058453
Other languages
French (fr)
Other versions
WO2010000854A2 (en
Inventor
Manfred Biebl
Renate Grassl
Monika Walter
Stefan Miller
Original Assignee
Biomerieux S.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux S.A. filed Critical Biomerieux S.A.
Priority to BRPI0913855A priority Critical patent/BRPI0913855B8/en
Priority to CN200980125992.8A priority patent/CN102099371B/en
Priority to EP09772554.3A priority patent/EP2310403B1/en
Priority to AU2009265666A priority patent/AU2009265666B2/en
Priority to US13/002,465 priority patent/US8609348B2/en
Publication of WO2010000854A2 publication Critical patent/WO2010000854A2/en
Publication of WO2010000854A3 publication Critical patent/WO2010000854A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10311—Siphoviridae
    • C12N2795/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2796/00—Viruses not covered by groups C12N2710/00 - C12N2795/00
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to bacteriophage adhesion proteins binding to the O-antigen of gram negative bacteria, lacking the ability of binding to a bacteriophage and of hydrolysing lipopolysaccharides. The invention further relates to nucleic acid molecules comprising a sequence encoding the proteins according to the present invention. In addition, the present invention relates to a method for generating bacteriophage adhesion proteins according to the present invention. The invention further relates to the use of said proteins and methods of detection, purification and enrichment of bacteria.
PCT/EP2009/058453 2008-07-04 2009-07-03 New bacteriophage adhesion proteins WO2010000854A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
BRPI0913855A BRPI0913855B8 (en) 2008-07-04 2009-07-03 bacteriophage adhesion protein, methods for detecting gram-negative bacteria, methods for selective purification and for enrichment or purification of gram-negative bacterial cells and/or cell components, and kit comprising said bacteriophage adhesion proteins
CN200980125992.8A CN102099371B (en) 2008-07-04 2009-07-03 New bacteriophage adhesion proteins
EP09772554.3A EP2310403B1 (en) 2008-07-04 2009-07-03 New bacteriophage adhesion proteins
AU2009265666A AU2009265666B2 (en) 2008-07-04 2009-07-03 New bacteriophage adhesion proteins
US13/002,465 US8609348B2 (en) 2008-07-04 2009-07-03 Bacteriophage adhesion proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08012152.8 2008-07-04
EP08012152A EP2141176B1 (en) 2008-07-04 2008-07-04 New bacteriophage adhesion proteins

Publications (2)

Publication Number Publication Date
WO2010000854A2 WO2010000854A2 (en) 2010-01-07
WO2010000854A3 true WO2010000854A3 (en) 2010-04-15

Family

ID=40119367

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/058453 WO2010000854A2 (en) 2008-07-04 2009-07-03 New bacteriophage adhesion proteins

Country Status (8)

Country Link
US (1) US8609348B2 (en)
EP (3) EP2141176B1 (en)
CN (2) CN102099371B (en)
AT (1) ATE520706T1 (en)
AU (1) AU2009265666B2 (en)
BR (1) BRPI0913855B8 (en)
ES (1) ES2371292T3 (en)
WO (1) WO2010000854A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012016107A1 (en) * 2010-07-30 2012-02-02 Sdix Methods and kits for detection of salmonella enteritidis and related serovars
JP5927730B2 (en) * 2013-04-11 2016-06-01 アサヒグループホールディングス株式会社 Screening method for lactic acid bacteria having immunomodulating action
CN107828784B (en) * 2017-12-26 2021-03-23 山东凯景生物技术有限公司 Kit and method for extracting and purifying escherichia coli DNA from complex sample
CN109541237A (en) * 2018-12-28 2019-03-29 吴江近岸蛋白质科技有限公司 The Determination of biological activity method of fibronectin
FR3110574A1 (en) * 2020-05-20 2021-11-26 Vetophage CAPTURE OF BACTERIA USING BACTERIOPHAGES OR BACTERIOPHAGE PROTEINS
FR3110703A1 (en) * 2020-05-20 2021-11-26 Vetophage DEVICE FOR DETECTION OF BACTERIA OF INTEREST
CN113881641B (en) * 2021-10-19 2023-06-23 广西大学 Escherichia coli phage EP01 and application thereof
CN115181165B (en) * 2022-06-22 2024-04-12 华中农业大学 Salmonella phage tail receptor binding protein RBP41 and application thereof in enrichment and detection of salmonella
KR20240086764A (en) * 2022-12-01 2024-06-19 서울과학기술대학교 산학협력단 Detection agent derived from bacteriophage tail spike protein that specifically binds to Escherichia coli O157:H7
CN116003531B (en) * 2022-12-28 2023-09-05 山东省农业科学院畜牧兽医研究所 Application of phage receptor binding protein PO86 in genotyping identification of E.coli O antigen

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001009370A2 (en) * 1999-07-30 2001-02-08 Profos Ag Detection and identification of bacterial strains
US20040121403A1 (en) * 2001-02-01 2004-06-24 Stefan Miller Detection and identification of groups of bacteria
US20040197833A1 (en) * 2003-04-03 2004-10-07 Danisco A/S Method for the enrichment of target cells by use of CBDs
US20040248298A1 (en) * 2001-06-24 2004-12-09 Michael Schutz Methods for purification of bacterial cells and components
WO2008121830A2 (en) * 2007-03-30 2008-10-09 National Research Council Of Canada Phage receptor binding proteins for antibacterial therapy and other novel uses

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7700729B2 (en) * 2006-05-15 2010-04-20 Avidbiotics Corporation Modified bacteriocins and methods for their use
DE102006061002A1 (en) * 2006-12-22 2008-06-26 Profos Ag Method and means for enrichment, removal and detection of gram-positive bacteria

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001009370A2 (en) * 1999-07-30 2001-02-08 Profos Ag Detection and identification of bacterial strains
US20040121403A1 (en) * 2001-02-01 2004-06-24 Stefan Miller Detection and identification of groups of bacteria
US20040248298A1 (en) * 2001-06-24 2004-12-09 Michael Schutz Methods for purification of bacterial cells and components
US20040197833A1 (en) * 2003-04-03 2004-10-07 Danisco A/S Method for the enrichment of target cells by use of CBDs
WO2008121830A2 (en) * 2007-03-30 2008-10-09 National Research Council Of Canada Phage receptor binding proteins for antibacterial therapy and other novel uses

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BAXA U ET AL: "INTERACTIONS OF PHAGE P22 TAILS WITH THEIR CELLULAR RECEPTOR, SALMONELLA O-ANTIGEN POLYSACCHARIDE", BIOPHYSICAL JOURNAL, NEW YORK, US, US, vol. 71, no. 4, 1 October 1996 (1996-10-01), pages 2040 - 2048, XP000613194, ISSN: 0006-3495 *
EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 215, no. 3, 1993, pages 653 - 661, XP002558169, ISSN: 0014-2956 *

Also Published As

Publication number Publication date
US8609348B2 (en) 2013-12-17
CN102099371A (en) 2011-06-15
EP2310403B1 (en) 2017-12-06
EP3124492A1 (en) 2017-02-01
AU2009265666B2 (en) 2013-09-05
CN105399801A (en) 2016-03-16
BRPI0913855B1 (en) 2020-11-10
BRPI0913855A2 (en) 2015-10-20
ES2371292T3 (en) 2011-12-29
EP2310403A2 (en) 2011-04-20
CN102099371B (en) 2016-03-30
BRPI0913855B8 (en) 2023-05-02
EP2141176B1 (en) 2011-08-17
EP2141176A1 (en) 2010-01-06
US20120122124A1 (en) 2012-05-17
CN105399801B (en) 2020-05-12
WO2010000854A2 (en) 2010-01-07
ATE520706T1 (en) 2011-09-15
AU2009265666A1 (en) 2010-01-07

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