WO2010000486A2

WO2010000486A2 - Bioreactor having a flat design

Info

Publication number: WO2010000486A2
Application number: PCT/EP2009/004827
Authority: WO
Inventors: Bernd KALTENHÄUSER
Original assignee: Kaltenhaeuser Bernd
Priority date: 2008-07-04
Filing date: 2009-07-03
Publication date: 2010-01-07
Also published as: DE102008031769A1; DE112009001499A5; DE102008031769B4; WO2010000486A3

Abstract

The invention relates to a bioreactor in which algae and other microorganisms, preferably microorganism which carry out photosynthesis, can be cultivated as well as to a method for the operation thereof and a method for the production thereof. The invention discloses in particular a bioreactor (100) for cultivating microorganisms, comprising a first (60) and a second part (50), a chamber system that is integrated into the bioreactor and comprises at least one growth chamber (5) for cultivating microorganisms and at least one distribution chamber (4) which is arranged adjacent to the at least one growth chamber and is connected to the latter via at least one opening (10). In order to configure the at least one opening (10), the extension of at least a first septum (8) disposed between the at least one distribution chamber (4) and the at least one growth chamber (5) is at least in places smaller than the clearance extension of the at least one growth chamber (5).

Description

Bioreactor in flat construction

The invention relates to a bioreactor in which algae and other microorganisms can be cultured, and a method for its operation and a method for its production. Due to the geometry of the bioreactor, at least one substantially cuboid growth chamber is formed, in which the preferably liquid culture medium can preferably be mixed with gas.

In bioreactors, biological metabolisms are carried out with enzymes or microorganisms, such as algae, bacteria, fungi or yeasts. The parameters temperature and light input as well as the pH value and the nutrient concentration of the solution are optimized for the ideal growth conditions or the reaction processes. For this, the nutrient liquid must usually be well mixed and mixed with a gas or gas mixture. These two processes can be combined by gas entry into the liquid with each other. The reaction surface is increased and the heat dissipated. The design of a bioreactor depends on its field of application and must therefore take into account the specific requirements of the biological system used.

For the cultivation of phototrophic microorganisms so-called photobioreactors are used. Airlift photobioreactors are particularly suitable for cultivating pho- totropic microorganisms at high cell density. The airlift photobioreactor often has a tower-shaped reactor vessel, in which by introducing a gas or gas mixture within a structurally determined loop, a liquid circulation is generated. Thus, in the airlift reactor, a fumigated and a non-gassed zone, which are connected on the ground and head side with each other. By the pressure difference, a pumping action is achieved, which leads in a liquid flow through the two zones. Since the mixing of the reactor medium is caused exclusively by the gas supply, in this photobioreactor design, a good mixing and a high gas-liquid mass transfer in comparison with other designs possible. Airlift bioreactors are vertically oriented to provide a high volume to volume ratio To achieve footprint. Such an airlift photobioreactor is described for example in DE 199 16 597.

Since it is necessary for the cultivation of phototrophic microorganisms to have a high intensity of light, in particular also into the depth of the reactor, photobioreactors preferably have a high surface to volume ratio. This leads to a high material usage, which is relatively complicated and cost-intensive due to the materials used such as glass.

Furthermore, usually elaborate and / or expensive scaffolding or supporting structures are used, where the reactors are suspended or attached. The height of the reactors (often several meters) determines the energy required to introduce the gas into the liquid from the height of the water column in the reactor.

In view of the above, it will be apparent to those skilled in the art from this disclosure that there is a need for an improved bioreactor. This invention addresses this need in the art as well as other needs that will become apparent to those skilled in the art from this disclosure.

Summary of the invention

The objects resulting from the above can essentially be achieved by a bioreactor for the cultivation of microorganisms according to claim 1 and a method for culturing microorganisms according to claim 39. In particular, the bioreactor according to the invention comprises a first and a second part, with a in the bioreactor integrated chamber system of at least one growth chamber for culturing microorganisms and at least one, adjacent to the at least one growth chamber arranged distribution chamber which is connected to the at least one growth chamber via at least one opening, in particular with these microorganisms, cultivation medium and / or preferably to supply at least one gas, wherein for the formation of the at least one opening, the expansion (height) of at least one arranged between the at least one distribution chamber and the at least one growth chamber first septum st in places smaller than the clear extension (height) of the at least one growth chamber.

The respective dimensions correspond to a height dimension during operation of the bioreactor.

A preferred embodiment of the bioreactor comprises a first and a second part, with at least one feed and at least one discharge chamber for a culture medium, microorganisms and / or preferably at least one gas, wherein the at least one feed chamber with the at least one discharge chamber over at least one, The expansion (height) of at least one of the at least one distribution chamber bounding and toward the growth chamber oriented first septa (Längssepten) at least in places less than the clear dimension (height ) is the at least one growth chamber and wherein the distribution chamber limiting first septa (Längssepten) are attached to the second part.

An advantage of the bioreactor according to the invention is that an extremely flat compared to existing bioreactors construction can be achieved because the bioreactor according to the invention is to be arranged substantially horizontally. This minimizes the energy required to introduce the gas into the culture medium and allows the reactor plates to be laid in a simple and fast manner.

Furthermore, the bioreactor according to the invention is self-supporting due to its geometry and does not have to be suspended or fastened to complex and / or expensive scaffolds or supporting structures. Preferably by the water and gas pressure, it remains stable in shape.

The basic bodies in the form of the first and second parts, including the septum or septa, can be produced inexpensively in a mass production process such as injection molding, extrusion blow molding or deep drawing and can subsequently or simultaneously be glued together, welded to ultrasound or radio frequency or otherwise be connected to each other. To change the production, the septa may also be hollow inside. The bioreactor according to the invention is preferably translucent, but may also be partially or impermeable to light as needed. In addition, it is possible to manufacture the first and / or the second part and / or at least one septum at least partially from a wavelength-shifting material and / or to coat it with an antireflection coating and / or at least partially to provide the photosynthesis activity increase.

Furthermore, the bioreactor can be made of a material or with a material, such. PTFE, which prevents adhesion of microorganisms to the bioreactor.

The cultured microorganisms can preferably be processed into biofuel, feed, food supplements and products for the pharmaceutical, chemical or cosmetic industry. In addition, the bioreactor can also be used for purification. As a result, the microorganisms can be supplied with nutrients.

The inner shape of the bioreactor is essentially determined by septa. Depending on the embodiment, these can be referred to as longitudinal and transverse septa. These can advantageously contribute to the mechanical stability of the bioreactor. Their arrangement results in essentially cuboid or trough-shaped growth chambers. In these, the microorganisms are cultivated.

A particularly preferred embodiment of the bioreactor consists of a growth chamber and a distribution chamber arranged adjacent thereto, which can supply the growth chamber with a preferably liquid culture medium, microorganisms and / or preferably at least one gas. The distribution chamber is separated from the growth chamber by at least a first septum. This first septum is arranged on the second part of the bioreactor and its extent (height) is at least in places smaller than the clear extent (height) of the growth chamber. This results in between the respective distribution chamber and the respective growth chamber at least one oriented to the first part opening (opening gaps or holes), through which the preferably liquid culture medium, the microorganism men and / or preferably at least one gas in the growth chamber (s) can get.

A liquid and / or gas permeable material may also be arranged in and / or at the at least one opening, such as e.g. a porous ceramic.

If necessary, the common lines for culture medium, microorganisms and / or preferably at least one gas can also be separated and / or attached to the periphery of the bioreactor (in operation above and / or below and / or laterally on the bioreactor) or inside the bioreactor become. Preferably, these are supply, distribution, additional and / or discharge chambers which can supply other chambers in the bioreactor with cultivation medium, microorganisms and / or gas and / or can discharge them from the bioreactor.

In operation, both culture medium, microorganisms and / or preferably at least one gas in one dimension (direction x, FIG. 4) can pass from the distribution chamber through the at least one opening into the wax chamber and then rise substantially vertically (direction z, FIG. 4). As a result, cultivation medium, microorganisms and / or preferably at least one gas are mixed with one another. In addition, culture medium and / or microorganisms can be set in cylindrical rotation, whereby each organism is only on the surface for a short time in order to absorb light (flashing light effect). In this case, it is possible to optimize the growth chambers substantially cylindrical or with a different geometry (FIG. 3).

From the growth chamber culture medium, microorganisms and gas are discharged from the bioreactor.

The connections for culture medium, microorganisms and / or at least one gas are preferably attached directly to the distribution and the growth chamber and thus integrated into the bioreactor. If further chambers, such as inlet and / or outlet chambers, are integrated into the bioreactor, the connections for culture medium, microorganisms and / or preferably at least one gas are preferably attached arranged this. In this case, the connections may be provided with a device to be connected to hoses, lines or other bioreactors.

Should a soluble gas, which is in particular carbon dioxide, be required for the cultivation, this can also be dissolved outside the bioreactor in the cultivation medium. In this case, or in the case where no gas is needed, the cylindrical rotation in the at least one growth chamber may be generated by the culture medium itself when it is pressed and / or sucked from the distribution chamber into the growth chamber.

Furthermore, it is possible to integrate several distribution and / or growth chambers in the bioreactor and to interconnect serially and / or in parallel with each other.

In the case of a serial connection, a growth chamber is preferably followed by a further distribution chamber which can supply a further growth chamber with cultivation medium, microorganisms and / or preferably at least one gas.

The growth chamber and the serial distribution chamber are separated by at least one second septum. This at least one second septum is arranged on the first part of the bioreactor and its extent (height) is at least in places smaller than the clear extent (height) of the growth chamber. This results in between the respective growth chamber and the respective distribution chamber at least one oriented to the second part opening (opening gaps or holes) through which culture medium, microorganisms and / or preferably at least one gas can get into the distribution chamber.

In this case, cultivation medium, microorganisms and / or at least one gas in one dimension (direction x, FIG. 8) can pass from the distribution chamber through the at least one opening into the growth chamber and then rise substantially vertically (direction z, FIG. 8). As a result, cultivation medium, microorganisms and / or at least one gas are mixed with each other. In addition, culture medium and / or microorganisms are set in cylindrical rotation, through which each organism is only on the surface for a short time in order to absorb light (flashing light effect). From the growth chamber culture medium, microorganisms and / or at least one gas, preferably in the same dimension (direction x, FIG. 8), through the at least one opening in the series-following distribution chamber. From the serial last growth chamber culture medium, microorganisms and / or preferably at least one gas are discharged from the bioreactor.

In the case of a parallel interconnection of a plurality of distribution and / or growth chambers, these are preferably connected to one another by at least one supply line and / or at least one discharge chamber.

From the supply chamber branch off the distribution chambers. These distribute cultivation medium, microorganisms and / or at least one gas to the growth chambers adjacent to them, wherein adjacent growth chambers are preferably at least locally separated from one another by at least one third septum (longitudinal septum). Preferably, each distribution chamber supplies two symmetrically arranged growth chambers, but only one can be supplied to optimize the turbulence of the cultivation medium. In this case, the distribution chamber is bounded on one side by a Längsseptum.

By at least a fourth septum (transverse septum) creates a supply chamber for culture medium, microorganisms and / or preferably at least one gas. At least one further fifth septum (transverse septum) for culture medium, microorganisms and / or preferably at least one gas produces a discharge chamber from the bioreactor. The at least one fourth septum completely separates the growth chambers from the supply chamber. The at least one fifth septum completely separates the distribution chambers from the discharge chamber, but forms the second

Partially oriented openings (opening gaps or holes) between the growth chambers and the discharge chamber.

In this case, cultivation medium, microorganisms and / or preferably at least one gas in one dimension (direction x, FIG. 12) can be introduced into the growth chamber (s) and then rise substantially vertically (direction z, FIG. 12). From there they can be discharged via another axis (direction y, FIG. 12). Furthermore, it is possible to arrange the growth chambers in one plane and to arrange the distribution chambers in another, essentially parallel plane. Likewise, the distribution chambers can be arranged in one plane and the growth chambers in the same or another, substantially parallel plane, while the at least one supply chamber and / or the at least one discharge chamber arranged in another plane or within the distribution and / or growth chambers are.

Other septa may also be added so that the evacuation chamber is extended to the area between two growth chambers. In this case, culture medium, microorganisms, and / or at least one gas, after ascending in the z-direction, first pass across the x-direction (substantially horizontally) into the discharge chamber, and are finally discharged into the y-direction (substantially horizontal). Likewise, the supply chambers may be identical to the distribution chambers.

Since the extent (height) of the first septa (Längssepten) is at least locally smaller than the clear dimension (height) of the growth chamber, formed between the respective distribution chamber and the respective growth chamber at least one oriented to the first part opening (opening gaps or holes), through which Culture medium, microorganisms and / or preferably at least one gas can get into the growth chambers.

The other side of the growth chambers, which does not adjoin the associated distribution chamber, is preferably closed by third septa (longitudinal septa) which, like some transverse septa, are preferably connected at least in places to both the first and the second part of the bioreactor. You can before the Aneinanderbefesti- gene, preferably joining, the two reactor halves on the first or on the second

Part can be arranged, or they can be distributed to the two reactor halves and only by the Aneinanderbefestigen, preferably joining finished.

In the growth chambers, the gas may bubble in the direction of the second part (direction z, FIG. 12). As a result, cultivation medium, microorganisms and / or preferably at least one gas are mixed with one another. In addition, a circulation of microorganisms, which cause each organism to remain on the surface for only a short time in order to absorb light (flashing light effect).

The fifth septa (transverse septa) which separate the growth chamber (s) from the discharge chamber are preferably attached to the first part and their extent (height) corresponds only in places to the clear extent (height) of the growth chamber. This results in at least one orifice oriented toward the second part (opening gaps or holes) through which the preferably liquid culture medium, microorganisms and / or preferably at least one gas can pass from the growth chamber into the discharge chamber. These fifth septa also prevent flushing of culture medium and algae into the discharge chamber. The fifth septa completely separate the distribution chamber (s) from the discharge chamber.

The connections for culture medium, microorganisms and / or at least one gas are preferably attached directly to the feed and discharge chambers and thus integrated into the bioreactor.

It is also possible for at least one serially interconnected chamber system and / or at least one chamber system connected in parallel to be interleaved and / or interconnected in parallel and / or in series with one another.

In all configurations described above, septa may have cross struts or stronger locations for stabilization. Likewise, they may be stabilized by their own shape (e.g., thicker at the first and second parts, thinner at the inside).

It is also possible to arrange further septa on one part, opposite to first or second septa, which are arranged on the other part of the bioreactor, in order to modify the liquid and the gas flow.

The connections for culture medium, microorganisms and / or preferably at least one gas and / or the outer walls of the bioreactor can be attached to the first or second part prior to bonding together, preferably joining the two reactor halves, or they can be distributed over the two reactor halves and only by the Aneinandefefigen, preferably Zusammenugen be completed.

For the fumigation preferably air, carbon dioxide-enriched air or pure carbon dioxide is used. However, if required, any other gas or gas mixture can be used.

The bioreactor can be connected in series and / or in parallel with other reactors in order to form a large-area plant and / or to optimize the pressure conditions in the bioreactor. The concept is preferably designed for large-scale systems. The bioreactor can also be used individually. Large-area plants are preferably used on otherwise unusable surfaces, such as e.g. Deserts, or installed at sea.

The width and length of a bioreactor can each be from less than a meter to several kilometers. The height is preferably in the range of a few centimeters, but it can also be from less than 1 mm to over one meter. Due to the low height of the entry of gas in the culture medium is possible with little energy. In this case, all length ratios between height, width and length of the biorector are possible, in particular, the aspect ratios wherein the ratio of width to length of the bioreactor is from 1: 100,000 to 100,000: 1 and the ratio of length to height of 1,000,000: 1 to 0.1: 1.

By the gas, which is in operation above the cultivation medium and below the second part (cover part), a greenhouse effect can be achieved and / or enhanced in the bioreactor.

Preferably, the bioreactor consists of two reactor halves or half bodies. Preferably, one half of the reactor comprises the first part (bottom part) and the other half of the reactor comprises the second part (cover part) together with at least one septum. It can also do more

Layers or bodies are used, for example, to produce other feeds or discharges for culture medium, microorganisms and / or at least one gas. Holes or retaining rings may be provided at the edge of the bioreactor for attaching or connecting the bioreactors to the floor.

The bioreactor is preferably made of a translucent plastic such as PET, PMMA or PVC, but glass, plexiglass or other materials may also be used.

The material of the bioreactor can be soft to avoid tensions, or even firm, to compensate for uneven floors. For this, the bioreactor can also be provided with feet or another base.

In addition, the bioreactor may comprise light pipe elements to direct light into the interior of the bioreactor, wherein the light pipe elements preferably comprise glass fibers.

Preferably, in operation preferably the at least one gas located in a distribution chamber maintains the water column of the culture medium by positive pressure, preferably in the corresponding growth chamber, at a certain level (in a certain position or height).

The supply lines for culture medium, microorganisms and / or at least one gas are preferably integrated into the bioreactor and not separately, e.g. through hoses. If hoses are used, they can have any shape and be rigid or flexible.

In operation of the bioreactor, the first part is preferably a bottom part and the second part is preferably a cover part. During operation of the bioreactor, the second part is thus preferably arranged above the first part.

The chamber system in the bioreactor, which is produced by the septa, represents a flow-guiding device for the cultivation medium, the microorganisms and / or the preferably at least one gas. On the outside of the first and / or the second part of the bioreactor, an additional protruding collar may be attached, at which the two parts can be more easily connected to each other or connected in any other way.

Further advantageous embodiments of the invention will become apparent from the dependent claims.

The invention will now be described in more detail with reference to the accompanying figures 1 to 12 and the associated embodiments.

Figure 1 shows a bottom part, chambers and septa of a bioreactor according to a first preferred embodiment;

FIG. 2 shows a bioreactor shown unfolded according to FIG. 1;

Figure 3 shows schematically a part of a section through the bioreactor according to Figure 1 along the axis xl - xl in Figure 1;

FIG. 4 shows a schematic representation of the flow direction of the cultivation medium, the microorganisms and / or the preferably at least one gas in a bioreactor according to FIG. 1;

FIG. 5 shows a bottom part, chambers and septa of a bioreactor according to a second preferred embodiment in a serial connection of growth and distribution chambers;

FIG. 6 shows an unfolded bioreactor according to FIG. 5;

Figure 7 shows schematically a part of a section through the bioreactor according to Figure 5 along the axis x2 - x2 in Figure 5;

FIG. 8 shows a schematic representation of the flow direction of the culture medium, the microorganisms and / or the preferably at least one gas in a bioreactor according to FIG. 5; FIG. 9 shows a bottom part, chambers and septa of a bioreactor according to a third preferred exemplary embodiment with a parallel connection of growth and distribution chambers;

FIG. 10 shows an unfolded bioreactor according to FIG. 9;

Figure 11 shows schematically a part of a section through the bioreactor according to Figure 9 along the axis x3 - x3 in Figure 9; and

FIG. 12 shows a schematic representation of the flow direction of the culture medium, of the microorganisms and / or of the preferably at least one gas in a bioreactor according to FIG. 9.

In the following selected embodiments of the invention will be described with reference to the figures. It will be apparent to those skilled in the art from this disclosure that the following descriptions of the embodiments of the invention are provided for illustration only, and not for the purpose of limiting the invention as defined by the appended claims and their equivalents.

FIG. 1 shows a first preferred exemplary embodiment of the bioreactor 100 in the assembled state. For the sake of clarity, the second part 50 (see FIGS. 2, 3) has been omitted, so that the at least one first septum 8 and the chambers 4, 5 are visible. The first part 60 constitutes a bottom part during operation, whereas the second part 50 represents a cover part.

As can be seen in FIG. 1, a distribution chamber 4 is separated from a growth chamber 5 by at least the first septum 8. This first septum 8 has at least in places a smaller extent / height than the clear extent / height of the growth chamber 5. The resulting at least one bottom-side (oriented to the first part 60) opening 10 between the distribution chamber 4 and the growth chamber 5 is also shown , The bioreactor according to the invention is preferably operated in the depicted orientation, ie in a substantially horizontal orientation. Thus, the extent A of the first septum 8 shown in Figure 3 (substantially orthogonal to the second part 50) may be referred to as height. Similarly, the extent H of the growth chamber 5 (substantially orthogonal to the first part 60) may be referred to as clear height.

Also shown in FIG. 1 are connections for culture medium, microorganisms and / or preferably at least one gas 1, 12, which are arranged on the distribution or growth chamber 5. Furthermore, an axis xl -xl is shown.

Figure 2 shows the embodiment of Figure 1 in disassembled / unfolded state. By folding and connecting, preferably gluing and / or welding of the two reactor halves, the bioreactor 100 according to FIG. 1 is formed. The first septum 8 is attached to the cover part 50. The first septum 8 is disposed between the distribution chamber 4 and the growth chamber 5 so as to longitudinally separate the distribution chamber 4 and the growth chamber 5 from each other.

FIG. 3 schematically shows a part of a section through the bioreactor 100 along the axis x1-x1 in FIG. 1. As can be seen particularly clearly in FIG. 3, the first septum 8 separates the distribution chamber 4 from the growth chamber 5. The height A of FIG However, the first septum 8 is at least in places less than the clear height H of the growth chamber 5. The resulting at least one bottom side (oriented toward the first part 60) opening 10 between the distribution chamber 4 and the growth chambers 5 is also shown. By means of this opening 10 oriented toward the bottom part 60, the growth chamber 5 can be supplied with microorganisms, cultivation medium and preferably at least one gas.

In the bioreactor according to this embodiment, the first septum 8 is arranged on the cover part 50, the arrangement preferably taking place before the two reactor halves are connected to one another. The first septum 8 is preferably on the cover side (toward the second part 50) stronger than the bottom side (toward the first part 60). FIG. 4 shows a representation of the flow direction of the culture medium, the microorganisms and / or the preferably at least one gas in a bioreactor according to FIG. 1. The culture medium, the microorganisms and / or the preferably at least one gas flow by pressure and / or suction effect by a suitable pressurizing means, preferably a pump, in x-direction (ie substantially horizontally) through the at least one opening 10 between the first septum 8 and the bottom part 60 from the distribution chamber 4 into the growth chamber 5. There the gas rises in the z-direction (ie essentially vertical) in the direction of the cover part 50 and puts the cultivation medium and / or the microorganisms in the growth chamber 5 in a roll-shaped rotation.

Figure 5 shows a second preferred embodiment of the bioreactor 100. For the first preferred embodiment identical or substantially identical parts are provided with identical reference numerals, so that can be dispensed with repetitive descriptions. It will be understood that insofar as the descriptions of the first preferred embodiment are to be applied mutatis mutandis to the second preferred embodiment.

FIG. 5 shows a bioreactor for serial shading of the growth and distribution chambers 4 in the assembled state. For the sake of clarity, the second part 50 (see FIGS. 6, 7) has been omitted, so that a large number of first and second septa 8, 13 and a large number of chambers 4, 5 are visible.

In the preferred embodiment shown here, four growth chambers 5 are shown with four associated distribution chambers 4, i. each distribution chamber supplies a growth chamber. It will be appreciated that more or fewer growth and distribution chambers may be provided.

In addition, an optional discharge chamber 3 is shown, which is connected to the serial last growth chamber 5.

The first septa 8 separate the distribution chambers 4 from the associated growth chambers 5, similar to the first preferred embodiment. These first septa 8 have in places a height A, which is less than the clear height H of the growing chambers 5 is (see Figure 7). This results in at least one bottom-side (oriented to the first part 60) opening 10 between the distribution chambers 4 and the associated growth chambers. 5

The second septa 13 separate the growth chambers 5 from the serial distribution chambers 4. These second septa 13 have a height B (see FIG. 7) which is at least locally smaller than the clear height H of the growth chambers 5. This results in at least one cover-side (oriented to the second part 50) opening 14 between a respective growth chamber 5 and the distribution chamber 4 following this serially.

Likewise, connections for culture medium, microorganisms and / or preferably at least one gas 1, 12, which are arranged on the series-first distribution chamber 4 or the discharge chamber 3, can be seen. Furthermore, an axis x2 -x2 is shown.

Figure 6 shows the embodiment of Figure 5 in disassembled / unfolded state. By folding and connecting, preferably gluing and / or welding of the two reactor halves, the bioreactor 100 according to FIG. 5 is formed. The second septa 13 are arranged here on the bottom part 60. The first septa 8 are arranged on the cover part 50. Through the first and second septa 8, 13, the distribution chambers 4 are longitudinally limited.

FIG. 7 shows schematically a part of a section through the bioreactor 100 in the case of a serial connection of the growth and distribution chambers 4 along the axis x2-x2. Here, the bottom and lid part 60, 50 are shown connected to each other. The first septa 8 separate the distribution chambers 4 from the associated growth chambers 5. The height of the first septa 8 is at least locally less than the clear height H of the growth chambers 5. This results in openings 10 between the distribution chambers 4 and the associated growth chambers. 5

In FIG. 7, the first septa 8 are attached to the cover part 50, the attachment preferably taking place before the two reactor halves are connected to one another. The first septa 8 are preferably stronger on the lid side than on the bottom side. The second septa 13 separate the growth chambers 5 from the serial distribution chambers 4. The height B of the second septa 13 is at least locally less than the clear height H of the growth chambers 5. This results in cover-side (oriented to the second part 50) openings 14 between the growth chambers 5 and the serial distribution chambers 4.

In the figure 7, the second septa 13 are arranged on the bottom part 60, wherein preferably the attachment takes place before the two reactor halves are connected to each other. The second septa 13 are preferably stronger on the bottom side than on the lid side.

Since the bioreactor of the invention is preferably operated in the depicted orientation, i. in a substantially horizontal orientation, the extent B of the second septum 13 (substantially orthogonal to the first portion 60) may be referred to as height B. Similarly, the clear dimension H of the growth chambers 5 (substantially orthogonal to the first part 60) may be referred to as the clear height H.

FIG. 8 shows a representation of the flow direction of the culture medium, of the microorganisms and / or of preferably at least one gas in a bioreactor in the case of a serial connection of the growth and distribution chambers 4 according to FIG. 5. This flow direction is shown schematically at several growth chambers 5. The culture medium, the microorganisms and / or the preferably at least one gas flow in the x-direction (ie substantially horizontally) through the respective bottom-side opening by pressure and / or suction effect, which are generated by a suitable pressurizing means, preferably a pump 10 from a distribution chamber 4 into the associated growth chamber 5. There, the gas rises in the z-direction (ie essentially vertically) in the direction of the cover part and puts the cultivation medium and / or the microorganisms in the growth chamber 5 in a roll-shaped rotation. From the growth chamber 5, culture medium, microorganisms and / or preferably at least one gas pass in the x-direction (i.e., substantially horizontally) through the cover-side opening 14 into the serial subsequent distribution chamber 4.

FIG. 9 shows a third preferred exemplary embodiment of the bioreactor 100 with a parallel connection of growth and distribution chambers 4 in the assembled state. Identical or identical to the first and / or second preferred embodiment Substantially identical parts are provided with identical reference numerals so that repetitive descriptions can be dispensed with. It will be appreciated that insofar as the descriptions of the first and / or second preferred embodiment are to be applied mutatis mutandis to the third preferred embodiment.

In FIG. 9, for the sake of clarity, the second part 50 (see FIGS. 10, 11) has been omitted, such that a plurality of first, third, fourth and fifth septa 6, 7, 8, 9 and a multiplicity of chambers 2, 3, 4 , 5 are visible.

In the embodiment shown here, six growth chambers 5 are shown with three associated distribution chambers 4, i. each distribution chamber supplies two growth chambers arranged adjacent thereto. It will be appreciated that more or fewer growth and distribution chambers may be provided. Each two adjacent growth chambers are preferably at least locally separated from one another by a third septum 7 (longitudinal septum).

The fourth septa 9 (cross spicers) completely separate the supply chamber 2 from the growth chambers 5. Since the fourth septa 9 provide openings, connections are formed from the supply chamber 2 to the distribution chambers 4 to culture medium, microorganisms and / or preferably at least one gas to initiate the distribution chambers 4. The distribution chambers 4 are separated by the first septa 8 from the adjacent growth chambers (5).

The growth chambers 5 are separated from the discharge chamber 3 by the at least one fifth septum 6 (transverse septum). This at least one fifth septum 6 has in places a height which is less than the clear height H of the growth chambers 5. This results in cover-side (oriented to the second part 50) openings 1 1 between the growth chambers 5 and the discharge chamber 3 to ausgereiten cultivation medium, microorganisms and / or preferably at least one gas from the growth chambers 5. Also, liquid and gas connections 1, 12, which are attached to the inlet 2 and discharge chamber 3, can be seen. Furthermore, an axis x3 -x3 is shown. FIG. 10 shows the exemplary embodiment according to FIG. 9 in disassembled / unfolded state. By folding and connecting, preferably gluing and / or welding of the two reactor halves, the bioreactor 100 according to FIG. 9 is formed. The fifth and third septa 6, 7 are arranged here on the bottom part 60, the first and fourth septa 8, 9 on the cover part 50 Through the first septa 8, the distribution chambers 4 are delimited longitudinally.

Figure 11 shows schematically a part of a section through the bioreactor 100 with a parallel interconnection of the growth and distribution chambers 4 along the axis x3 - x3 in Figure 9. Here, the bottom and lid part 60, 50 are connected to each other. Two first septa 8 delimit a distribution chamber 4. The height A of the first septa 8 is at least locally smaller than the clear height H of the growth chambers 5. This results in bottom-side (oriented for the first part 60) openings 10 between the distribution chamber 4 and the respective growth chambers 5th

In each case two growth chambers 5 are preferably separated from one another by at least a third septum 7. This third septum 7 connects the bottom part 60 with the cover part 50 at least in places and thus advantageously stabilizes the bioreactor. As can be seen in FIG. 11, the third septa 7 are arranged on the bottom part 60, the arrangement preferably taking place before the two reactor halves are connected to one another. The third septa 7 are preferably on the bottom side stronger than the lid side. The first septa 8 are preferably arranged on the cover part 50, wherein preferably the arrangement takes place before the two reactor halves are connected to each other. Thus, the third septa 7 are preferably on the bottom side stronger than the lid side.

FIG. 12 shows a representation of the flow direction of the cultivation medium, the microorganisms and / or the preferably at least one gas in a bioreactor 100 with a parallel connection of the growth and distribution chambers 4 according to FIG. 9. This flow direction is shown schematically on a growth chamber 5 , The culture medium, the microorganisms and / or the preferably at least one gas flow through pressure and / or suction, which are generated by a suitable biasing means, preferably a pump, in the x-direction (ie, substantially horizontal) through the feed chamber 2 and are directed from there in y-direction (ie, substantially horizontal) in the distribution chamber 4. From the Partial chamber 4 flow the culture medium, the microorganisms and / or preferably at least one gas through the bottom opening 10 against the x direction in a growth chamber 5. There, the gas in the z-direction (ie, substantially vertically) along in the direction of the lid part 50 and puts the culture medium in cylindrical rotation. From there, culture medium, microorganisms and / or preferably at least one gas finally pass through the opening 11 in the y-direction (ie essentially horizontally) from the growth chamber into the discharge chamber 3.

While only selected embodiments have been chosen to illustrate the present invention, it will be apparent to those skilled in the art from this disclosure that various changes and modifications can be made without departing from the scope of the invention as defined in the appended claims and their equivalents. The structures and functions of one embodiment may be incorporated into another. Thus, the foregoing descriptions of the embodiments according to the present invention are provided for illustration only, and not for the purpose of limiting the invention as defined by the appended claims and their equivalents.

Claims

1. A bioreactor (100) for the cultivation of microorganisms, comprising a first (60) and a second part (50), with a chamber system integrated into the bioreactor from at least one growth chamber (5) for cultivating microorganisms and at least one, adjacent to the at least one growth chamber arranged distribution chamber (4) which is connected to the growth chamber via at least one opening (10), wherein for forming the at least one opening (10) the extent (A) of at least one between the at least one distribution chamber ( 4) and the at least one growth chamber (5) arranged first septum (8) is at least in places smaller than the clear dimension (H) of the at least one growth chamber (5).

2. bioreactor (100) according to claim 1, wherein the bioreactor (100) is provided in one piece or in two parts in the form of the first (60) and the second part (50) with at least a first septum (8), which connectable with each other are.

3. bioreactor (100) according to any one of the preceding claims, wherein the at least one first septum (8) on the second part (50) is arranged and the at least one opening (10) to the first part (60) is oriented.

4. bioreactor (100) according to any one of the preceding claims, wherein the first part (60) an additional septum opposite to the at least one first septum (8) is arranged to the position of the at least one opening (10) in the direction of the second part ( 50).

5. bioreactor (100) according to any one of the preceding claims, wherein in and / or at the at least one opening (10) a gas and / or liquid-permeable material is arranged.

6. bioreactor (100) according to any one of the preceding claims, wherein the bioreactor comprises at least one feed chamber (2) which is connected to the at least one distribution chamber (4), and / or at least one discharge chamber (3), which with the at least a growth chamber (5) is connected to cultivate tion medium, microorganisms and / or at least one gas into the at least one distribution chamber (4) to initiate and / or out of the at least one growth chamber (5).

7. bioreactor (100) according to any one of the preceding claims, wherein the bioreactor a plurality of distribution chambers (4) and / or a plurality of growth chambers (5).

8. bioreactor (100) according to any one of the preceding claims, wherein a plurality of distribution chambers (4) and / or a plurality of growth chambers (5) are connected in parallel and / or in series with each other.

9. bioreactor (100) according to any one of the preceding claims, wherein in the serial connection to a distribution chamber (4) a growth chamber (5) and in turn this follows a distribution chamber (4).

10. bioreactor (100) according to any one of the preceding claims, wherein the distribution (4) and the growth chambers (5) lie in one plane.

11. bioreactor (100) according to any one of the preceding claims, wherein the distribution (4) and the growth chambers (5) are arranged substantially horizontally in the operating state.

12. bioreactor (100) according to any one of the preceding claims, wherein the bioreactor comprises at least one supply chamber (2) which is connected to the first distribution chamber (4) of the serial connection and / or at least one discharge chamber (3), which with the last growth chamber (5) of the serial interconnection is connected.

13. bioreactor (100) according to any one of the preceding claims, wherein at least a second septum (13) is provided, with the at least one first septum (8) a

Distribution chamber (4) is formed and whose extension (B) is at least in places smaller than the clear dimension (H) of the at least one growth chamber (5).

14. bioreactor (100) according to any one of the preceding claims, wherein in each case a growth chamber (5) to the serial subsequent Verteilkammer (4) by the at least one second septum (13) is separated.

The bioreactor (100) of any one of the preceding claims, wherein the at least one second septum (13) and the at least one first septum (8) are disposed adjacent to each other such that the volume of the distribution chamber (4) defined by these septa is less than or equal to the volume of the growth chamber (5) to be supplied by this distribution chamber.

16. bioreactor (100) according to any one of the preceding claims, wherein the at least one second septum (13) on the first part (60) is arranged and at least one second part (50) oriented opening (14) between at least one growth chamber (5) the serially following distribution chamber (4) provides.

17. bioreactor (100) according to any one of the preceding claims, wherein a plurality of distribution chambers (4) parallel to each other and / or a plurality of growth chambers (5) parallel to each other and / or at least one distribution chamber (4) and at least one growth chamber (5) are connected in parallel ,

18. bioreactor (100) according to any one of the preceding claims, wherein at least one feed chamber (2) a plurality of distribution chambers (4) connected in parallel with each other to provide them with culture medium, microorganisms and / or preferably at least one gas.

19. bioreactor (100) according to any one of the preceding claims, wherein at least one discharge chamber (3) a plurality of growth chambers (5) connected in parallel with each other to derive culture medium, microorganisms and / or at least one gas from the growth chambers.

20. Bioreactor according to one of the preceding claims, wherein at least one growth chamber (5) and at least one distribution chamber (4) between the at least one feed line (2) and the at least one discharge chamber (3) are arranged.

21. Bioreactor according to one of the preceding claims, wherein in each case two growth chambers (5) are arranged directly adjacent to each other and from each two immediately adjacent growth chambers (5) are separated by a respective distribution chamber (4).

22. The bioreactor (100) according to any one of the preceding claims, wherein at least two first septa (8) are provided to form a distribution chamber (4).

23. Bioreactor according to one of the preceding claims, wherein a fifth septum (6) is provided which delimits the at least one discharge chamber (3) against the at least one distribution chamber (4) and against the at least one growth chamber (5) and at least in places the first (60) connects to the second part (50) so that the at least one distribution chamber (4) is closed relative to the discharge chamber (3).

24. Bioreactor according to one of the preceding claims, wherein the fifth septum (6) on the first part (60) is arranged and at least in places has an extent which is smaller than the clear dimension (H) of the at least one growth chamber (5).

25. bioreactor according to any one of the preceding claims, wherein at least one of the second part (50) oriented opening (11) between the at least one growth chamber (5) and the at least one discharge chamber (3) is provided by the fifth septum (6).

26. bioreactor according to any one of the preceding claims, wherein at least a fourth septum (9) is provided which limits the at least one supply chamber (2) against the at least one growth chamber (5) and / or the at least one distribution chamber (4) and at least in places connecting the first part (60) to the second part (50) such that the at least one growth chamber (5) is closed relative to the supply chamber (2) while the at least one distribution chamber (4) is connected through at least one opening to the at least one supply chamber ( 2) is connected.

27. bioreactor according to any one of the preceding claims, wherein the supply (2) and / or discharge chambers (3) in the form of tubes and / or tubes present.

28. Bioreactor (100) according to any one of the preceding claims, wherein the distribution chambers (4) lie in one plane and the growth chambers (5) in another, im

Lie substantially parallel plane.

29. Bioreactor (100) according to any one of the preceding claims, wherein the distribution chambers (4) are arranged in a plane and the growth chambers (5) are arranged in the same or another, substantially parallel plane, while the at least one feed chamber (2) and / or the at least one discharge chamber (3) are arranged in a different plane and / or within the distribution (4) and / or growth chambers (5).

30. bioreactor (100) according to any one of the preceding claims, wherein at least one serially interconnected Kammersystem according to one of the preceding claims and / or at least one parallel interconnected Kammersystem according to one of the preceding claims interleaved and / or connected in parallel and / or in series with each other.

31. Bioreactor according to one of the preceding claims, which is made of a translucent plastic such as PET, PMMA or PVC.

32. Bioreactor according to one of the preceding claims, wherein at least one of the first, second, third, fourth and fifth septa (6, 7, 8, 9, 13) at least in places the first (60) and the second part (50) to Stabilization of the bioreactor connects with each other.

33. Bioreactor according to one of the preceding claims, wherein the at least one distribution chamber (4) and the at least one growth chamber (5) are arranged so that an overpressure of a gas in the at least one distribution chamber (4) the water column of the cultivation medium in the at least a growth chamber (5) can keep at a certain level.

34. A bioreactor according to any one of the preceding claims, having light-guiding elements to direct light into the interior of the bioreactor, wherein the light-conducting elements preferably comprise glass fibers.

35. Bioreactor according to one of the preceding claims, which can be connected / connected in parallel with other bioreactors in series and / or in parallel to form a modular production plant.

36. Bioreactor according to one of the preceding claims, in which a gas layer can be arranged above the cultivation medium with which a greenhouse effect can be achieved and / or enhanced in the bioreactor.

37. Bioreactor according to one of the preceding claims, wherein the first and / or the second part (60, 50) and / or at least one septum at least partially made of a wavelength-shifting material and / or coated with such and / or at least partially with an anti-reflective coating are provided.

38. Bioreactor according to one of the preceding claims, wherein the first part (60) is a bottom part and the second part (50) is a cover part.

39. A method for cultivating microorganisms, which is carried out in particular with a bioreactor according to one of claims 1 to 38, wherein a cultivation medium, microorganisms and / or preferably at least one gas in at least one distribution chamber (4), from there through at least one opening ( 10) on the bottom side of at least one first septum (8) arranged between the at least one distribution chamber (4) and at least one growth chamber (5), into which at least one growth chamber (5) and from there in the direction of a cover part (50) are conveyed become.

40. The method for cultivating microorganisms according to claim 39, wherein the overpressure of a gas in the at least one distribution chamber (4) presses a culture medium located in the at least one distribution chamber (4) towards the at least one growth chamber (5) and as soon as the gas reaches the at least one opening (10) on the bottom side of the at least one first septum (8) reaches, passes through the at least one opening (10) in the at least one growth chamber (5) rises in this and preferably in the at least one growth chamber (5) located cultivation medium and / or the microorganisms in cylindrical rotation.

41. A method for culturing microorganisms according to any one of claims 39 and 40, wherein a cultivation medium, microorganisms and / or preferably at least one gas in a serially first distribution chamber (4), from there through the at least one opening (10) on the bottom side of the at least one first septum (8) into a growth chamber (5), from there in the direction of a cover part (50), through at least one cover side of a second septum (13) located opening (14) in a next distribution chamber (4), and preferably of be promoted there analogously to a serial last growth chamber (5).

42. Method for culturing microorganisms according to one of claims 39 to 41, wherein a cultivation medium, microorganisms and / or preferably at least one gas are conveyed into a supply chamber (2) and from there into a series-first distribution chamber (4) and / or from a serial last growth chamber (5) in a discharge chamber (3) are promoted.

43. A method for cultivating microorganisms according to any one of claims 39 to 42, wherein a cultivation medium and / or at least one gas in a feed chamber (2), from there into a distribution chamber (4), from there through the at least one opening (10). on the bottom side of the first septum (8) into a growth chamber (5) and from there in the direction of a cover part (50), are conveyed through at least one opening (11) on the cover side of a fifth septum (6) into a discharge chamber (8).

44. Method for cultivating microorganisms according to one of claims 39 to 43, wherein the transport of the cultivation medium, the microorganisms and / or the preferably at least one gas takes place by means of pressure and / or suction.

45. Method for cultivating microorganisms according to one of claims 39 to 44, in which the cultivation medium and / or the microorganisms in the at least one growth chamber (5) are set in cylindrical rotation by inflowing culture medium.

46. A method for producing a bioreactor according to any one of claims 1 to 38, wherein the first and second part (50, 60) together with septum / septa produced simultaneously in a deep-drawing process and thereby welded together.