WO2009152825A1 - Novel phenylimidazole derivatives as pde10a enzyme inhibitors - Google Patents

Novel phenylimidazole derivatives as pde10a enzyme inhibitors Download PDF

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Publication number
WO2009152825A1
WO2009152825A1 PCT/DK2009/050134 DK2009050134W WO2009152825A1 WO 2009152825 A1 WO2009152825 A1 WO 2009152825A1 DK 2009050134 W DK2009050134 W DK 2009050134W WO 2009152825 A1 WO2009152825 A1 WO 2009152825A1
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Prior art keywords
phenyl
imidazol
methyl
triazolo
dimethyl
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PCT/DK2009/050134
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French (fr)
Inventor
Andreas RITZÉN
Jan Kehler
Morten LANGGÅRD
Jacob Nielsen
John Paul Kilburn
Mohamed M. Farah
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H. Lundbeck A/S
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Priority to RS20140135A priority Critical patent/RS53226B/en
Priority to SI200930879T priority patent/SI2318394T1/en
Priority to CN200980132027.3A priority patent/CN102124002B/en
Priority to KR1020177002859A priority patent/KR20170016523A/en
Priority to EA201170061A priority patent/EA018880B1/en
Priority to PL09765461T priority patent/PL2318394T3/en
Priority to ES09765461.0T priority patent/ES2456349T3/en
Priority to MX2010014136A priority patent/MX2010014136A/en
Priority to CA2728335A priority patent/CA2728335C/en
Priority to KR1020177034302A priority patent/KR102025487B1/en
Priority to NZ589926A priority patent/NZ589926A/en
Priority to KR1020107028654A priority patent/KR101703938B1/en
Priority to JP2011513878A priority patent/JP5930714B2/en
Priority to UAA201015420A priority patent/UA102693C2/en
Priority to DK09765461.0T priority patent/DK2318394T3/en
Priority to BRPI0915383A priority patent/BRPI0915383B8/en
Priority to AU2009259209A priority patent/AU2009259209B2/en
Priority to EP09765461.0A priority patent/EP2318394B1/en
Publication of WO2009152825A1 publication Critical patent/WO2009152825A1/en
Priority to RS20140700A priority patent/RS53704B1/en
Priority to MA34464A priority patent/MA33367B1/en
Priority to NZ597203A priority patent/NZ597203A/en
Priority to PCT/DK2010/050147 priority patent/WO2010145668A1/en
Priority to DK10725373.4T priority patent/DK2443105T3/en
Priority to CA2765750A priority patent/CA2765750A1/en
Priority to CN201080037331.2A priority patent/CN102482262B/en
Priority to EA201270046A priority patent/EA021410B1/en
Priority to BRPI1015983A priority patent/BRPI1015983A2/en
Priority to SI201030841T priority patent/SI2443105T1/en
Priority to SG2011089919A priority patent/SG176680A1/en
Priority to US13/378,405 priority patent/US8841297B2/en
Priority to GEAP201012536A priority patent/GEP20146100B/en
Priority to MYPI2011005865A priority patent/MY156785A/en
Priority to ES10725373.4T priority patent/ES2527216T3/en
Priority to MX2011013400A priority patent/MX2011013400A/en
Priority to JP2012515356A priority patent/JP5768044B2/en
Priority to PT107253734T priority patent/PT2443105E/en
Priority to UAA201200499A priority patent/UA106378C2/en
Priority to PL10725373T priority patent/PL2443105T3/en
Priority to AU2010262190A priority patent/AU2010262190B2/en
Priority to EP10725373.4A priority patent/EP2443105B1/en
Priority to ARP100102165A priority patent/AR077144A1/en
Priority to TW099119840A priority patent/TW201100430A/en
Priority to IL209834A priority patent/IL209834A/en
Priority to ZA2011/00109A priority patent/ZA201100109B/en
Priority to TNP2011000612A priority patent/TN2011000612A1/en
Priority to IL216684A priority patent/IL216684A/en
Priority to CR20110654A priority patent/CR20110654A/en
Priority to GT201100322A priority patent/GT201100322A/en
Priority to ZA2011/09271A priority patent/ZA201109271B/en
Priority to CL2011003205A priority patent/CL2011003205A1/en
Priority to DO2011000397A priority patent/DOP2011000397A/en
Priority to HK12100229.9A priority patent/HK1159632A1/en
Priority to HK12112165.0A priority patent/HK1171446A1/en
Priority to HRP20140232AT priority patent/HRP20140232T1/en
Priority to HRP20141222AT priority patent/HRP20141222T1/en
Priority to CY20141101071T priority patent/CY1115971T1/en
Priority to SM201500003T priority patent/SMT201500003B/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
    • A61K31/4164—1,3-Diazoles
    • A61K31/4178—1,3-Diazoles not condensed 1,3-diazoles and containing further heterocyclic rings, e.g. pilocarpine, nitrofurantoin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
    • A61K31/4196—1,2,4-Triazoles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/4985—Pyrazines or piperazines ortho- or peri-condensed with heterocyclic ring systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/18—Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/30—Drugs for disorders of the nervous system for treating abuse or dependence
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/30—Drugs for disorders of the nervous system for treating abuse or dependence
    • A61P25/32—Alcohol-abuse
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
    • C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
    • C07D403/06—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
    • C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
    • C07D403/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
    • C07D471/04—Ortho-condensed systems

Definitions

  • the present invention provides compounds that are PDE10A enzyme inhibitors, and as such are useful to treat neurodegenerative and psychiatric disorders. Especially, the invention provides compounds that are highly selective for PDE10 over other PDE subtypes. The present invention also provides pharmaceutical compositions comprising compounds of the invention and methods of treating disorders using the compounds of the invention.
  • cyclic-adenosine monophosphate cyclic-adenosine monophosphate
  • cGMP cyclic-guanosine monophosphate
  • Intracellular cAMP and cGMP are generated by adenyl and guanyl cyclases, and are degraded by cyclic nucleotide phosphodiesterases (PDEs).
  • Intracellular levels of cAMP and cGMP are controlled by intracellular signaling, and stimulation/repression of adenyl and guanyl cyclases in response to GPCR activation is a well characterized way of controlling cyclic nucleotide concentrations (Antoni, F.A. Front.
  • cAMP and cGMP levels in turn control activity of cAMP- and cGMP-dependent kinases as well as other proteins with cyclic nucleotide response elements, which through subsequent phosphorylation of proteins and other processes regulate key neuronal functions such as synaptic transmission, neuronal differentiation and survival.
  • PDEs are a class of intracellular enzymes that regulate levels of cAMP and cGMP via hydrolysis of the cyclic nucleotides into their respective nucleotide monophosphates. Some PDEs degrade cAMP, some cGMP and some both. Most PDEs have a widespread expression and have roles in many tissues, while some are more tissue-specific.
  • Phosphodieasterase 1OA is a dual-specificity phosphodiesterase that can convert both cAMP to AMP and cGMP to GMP (Loughney, K. et al. Gene 1999, 234,
  • PDE10A is primarily expressed in the neurons in the striatum, n. accumbens and in the olfactory tubercle (Kotera, J. et al.
  • Mouse PDE10A is the first identified member of the PDE10 family of phosphodiesterases (Fujishige, K. et al. J. Biol. Chem. 1999, 274, 18438-18445 and Loughney, K. et al. Gene 1999, 234, 109-117) and N-terminal splice variants of both the rat and human genes have been identified (Kotera, J. et al. Biochem. Biophys. Res. Comm. 1999, 261, 551-557 and Fujishige, K. et al. Eur. J. Biochem. 1999, 266, 1118- 1127). There is a high degree of homology across species. PDE10A is uniquely localized in mammals relative to other PDE families.
  • mRNA for PDE10 is highly expressed in testis and brain (Fujishige, K. et al. Eur J Biochem. 1999, 266, 1118- 1127; Soderling, S. et al. Proc. Natl. Acad. Sci. 1999, 96, 7071-7076 and Loughney, K. et al. Gene 1999, 234,109-117). These studies indicate that within the brain, PDE10 expression is highest in the striatum (caudate and putamen), n. accumbens and olfactory tubercle. More recently, an analysis has been made of the expression pattern in rodent brain of PDE10A mRNA (Seeger, T.F. et al. Abst. Soc.
  • MSN medium spiny neurons
  • the MSN has a key role in the cortical-basal ganglia-thalamocortical loop, integrating convergent cortical/thalamic input, and sending this integrated information back to the cortex.
  • MSN express two functional classes of neurons: the Di class expressing Di dopamine receptors and the D2 class expressing D2 dopamine receptors.
  • the Di class of neurons is part of the 'direct' striatal output pathway, which broadly functions to facilitate behavioral responses.
  • the D2 class of neurons is part of the 'indirect' striatal output pathway, which functions to suppress behavioral responses that compete with those being facilitated by the 'direct' pathway. These competing pathways act like the brake and accelerator in a car.
  • the poverty of movement in Parkinson's disease results from over-activity of the 'indirect' pathway, whereas excess movement in disorders such as Huntington's disease represent over-activity of the direct pathway.
  • PDE10A regulation of cAMP and/or cGMP signaling in the dendritic compartment of these neurons may be involved in filtering the cortico/thalamic input into the MSN.
  • PDE10A may be involved in the regulation of GABA release in the substantia nigra and globus pallidus (Seeger, T. F. et al. Brain Research, 2003, 985, 113-126).
  • Dopamine D 2 receptor antagonism is well established in the treatment of schizophrenia. Since the 1950's, dopamine D 2 receptor antagonism has been the mainstay in psychosis treatment and all effective antipsychotic drugs antagonise D 2 receptors. The effects of D 2 are likely to be mediated primarily through neurons in the striatum, n. accumbens and olfactory tubercle, since these areas receive the densest dopaminergic projections and have the strongest expression of D 2 receptors (Konradi, C. and Heckers, S. Society of Biological Psychiatry, 2001 , 50, 729-742).
  • Dopamine D 2 receptor agonism leads to decrease in cAMP levels in the cells where it is expressed through adenylate cyclase inhibition, and this is a component of D 2 signalling (Stoof, J. C; Kebabian J. W. Nature 1981 , 294, 366-368 and Neve, K. A. et al. Journal of Receptors and Signal Transduction 2004, 24, 165-205).
  • D 2 receptor antagonism effectively increases cAMP levels, and this effect could be mimicked by inhibition of cAMP degrading phosphodiesterases.
  • PDE10A in this context, has the desired expression profile with high and relatively specific expression in neurons in striatum, n. accumbens and olfactory tubercle, PDE10A inhibition is likely to have effects similar to D 2 receptor antagonism and therefore have antipsychotic effects. While PDE10A inhibition is expected to mimic D 2 receptor antagonism in part, it might be expected to have a different profile.
  • the D2 receptor has signalling components besides cAMP (Neve, K. A. et al.
  • PDE10A inhibition may negatively modulate rather than directly antagonise dopamine signaling through D 2 receptors. This may reduce the risk of the extrapyrimidal side effects that are seen with strong D 2 antagonism. Conversely, PDE10A inhibition may have some effects not seen with D 2 receptor antagonism. PDE10A is also expressed in Di receptors expressing striatal neurons (Seeger, T. F. et al. Brain Research, 2003, 985, 113-126).
  • Di receptor agonism leads to stimulation of adenylate cyclase and resulting increase in cAMP levels
  • PDE10A inhibition is likely to also have effects that mimic Di receptor agonism.
  • PDE10A inhibition will not only increase cAMP in cells, but might also be expected to increase cGMP levels, since PDE10A is a dual specificity phosphodiesterase.
  • cGMP activates a number of target protein in cells like cAMP and also interacts with the cAMP signalling pathways.
  • PDE10A inhibition is likely to mimic D 2 receptor antagonism in part and therefore has antipsychotic effect, but the profile might differ from that observed with classical D 2 receptor antagonists.
  • the PDE10A inhibitor papaverine is shown to be active in several antipsychotic models.
  • Papaverine potentiated the cataleptic effect of the D 2 receptor antagonist haloperidol in rats, but did not cause catalepsy on its own (WO 03/093499).
  • Papaverine reduced hyperactivity in rats induced by PCP, while reduction of amphetamine induced hyperactivity was insignificant (WO 03/093499).
  • WO 03/093499 further discloses the use of selective PDE10 inhibitors for the treatment of associated neurologic and psychiatric disorders.
  • PDE10A inhibition reverses subchronic PCP-induced deficits in attentional set-shifting in rats (Rodefer et al. Eur. J. Neurosci. 2005, 4, 1070-1076). This model suggests that PDE10A inhibition might alleviate cognitive deficits associated with schizophrenia.
  • the tissue distribution of PDE10A indicates that PDE10A inhibitors can be used to raise levels of cAMP and/or cGMP within cells that express the PDE10 enzyme, especially neurons that comprise the basal ganglia, and the PDE10A inhibitors of the present invention would therefore be useful in treating a variety of associated neuropsychiatric conditions involving the basal ganglia such as neurological and psychiatric disorders, schizophrenia, bipolar disorder, obsessive compulsive disorder, and the like, and may have the benefit of not possessing unwanted side effects, which are associated with the current therapies on the market.
  • EP 1250923 discloses the use of selective PDE10 inhibitors in general, and papaverine in particular, for the treatment of certain neurologic and psychiatric disorders.
  • WO 05/113517 discloses benzodiazepine stereospecific compounds as inhibitors of phosphodiesterase, especially types 2 and 4, and the prevention and treatment of pathologies involving a central and/or peripheral disorder.
  • WO 02/88096 discloses benzodiazepine derivatives and their uses as inhibitors of phosphodiesterase, especially type 4 in the therapeutic field.
  • WO 04/41258 discloses benzodiazepinone derivatives and their uses as inhibitors of phosphodiesterase, especially type 2 in the therapeutic field.
  • Pyrrolodihydroisoquinolines and variants thereof are disclosed as inhibitors of PDE10 in WO 05/03129 and WO 05/02579.
  • Piperidinyl-substituted quinazolines and isoquinolines that serve as PDE10 inhibitors are disclosed in WO 05/82883.
  • WO 06/11040 discloses substituted quinazoline and isoquinoline compounds that serve as inhibitors of PDE10.
  • US 20050182079 discloses substituted tetrahydroisoquinolinyl derivatives of quinazoline and isoquinoline that serve as effective phosphodiesterase (PDE) inhibitors.
  • PDE phosphodiesterase
  • US 20060019975 discloses piperidine derivatives of quinazoline and isoquinoline that serve as effective phosphodiesterase (PDE) inhibitors.
  • US 20060019975 also relates to compounds that are selective inhibitors of PDE10.
  • WO 06/028957 discloses cinnoline derivatives as inhibitors of phosphodiesterase type 10 for the treatment of psychiatric and neurological syndromes.
  • the compounds of the invention may offer alternatives to current marketed treatments for neurodegenerative and/or psychiatric disorders, which are not efficacious in all patients. Hence, there remains a need for alternative methods of treatment.
  • the objective of the present invention is to provide compounds that are selective PDE10A enzyme inhibitors.
  • a further objective of the present invention is to provide compounds which have such activity, and which have improved solubility, metabolic stability and/or bioavailability compared to prior art compounds.
  • Another objective of the invention is to provide an effective treatment, in particular long- term treatment, of a human patient, without causing the side effects typically associated with current therapies for neurological and psychiatric disorders. Further objectives of the invention will become apparent upon reading the present specification.
  • the present invention relates to compounds of formula I:
  • HET is a heteroaromatic group of formula Il containing from 2 to 4 nitrogen atoms:
  • Y can be N or CH
  • Z can be N or C
  • HET may optionally be substituted with up to three substituents R7, R8 and R9 individually selected from H; Ci-C ⁇ alkyl such as Me; halogen such as chlorine and bromine; cyano; halo(Ci-C 6 )alkyl such as trifluoromethyl; aryl such as phenyl; alkoxy, preferably Ci-C ⁇ alkoxy, such as methoxy, dimethoxy, ethoxy, methoxy-ethoxy and ethoxy-methoxy, and C1-C6 hydroxyalkyl such as CH 2 CH 2 OH, and wherein * denotes the attachment point,
  • R1 is selected from H; Ci-C 6 alkyl such as methyl, ethyl, 1 -propyl, 2-propyl, isobutyl; d- C ⁇ alkyl(C 3 -C 8 )cycloalkyl such as cyclopropylmethyl; Ci-C ⁇ hydroxyalkyl such as hydroxyethyl; CH 2 CN; CH 2 C(O)NH 2 ; Ci-C ⁇ arylalkyl such as benzyl and 4-chlorobenzyl; and C 1 -C 6 alkyl-heterocycloalkyl such as tetrahydropyran-4-yl-methyl and 2-morpholin- 4-yl-ethyl; R2-R6 are each selected independently from H; C1-C6 alkoxy such as methoxy; and halogen such as chlorine or fluorine;
  • the invention relates to a compound of formula I in the form of a single tautomer or a polymorph.
  • the compound of formula I is selected among the specific compounds disclosed in the Experimental Section herein.
  • the invention further provides a compound of formula I, or a pharmaceutically acceptable acid addition salt thereof, for use as a medicament.
  • the present invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a therapeutically effective amount of a compound of formula I and a pharmaceutically acceptable carrier, diluent or excipient.
  • the invention further provides the use of a compound of formula I, or a pharmaceutically acceptable acid addition salt thereof, for the preparation of a medicament for the treatment of a neurodegenerative or psychiatric disorder.
  • the present invention provides a method of treating a subject suffering from a neurodegenerative disorder, comprising administering to the subject a therapeutically effective amount of a compound of formula I.
  • the present invention provides a method of treating a subject suffering from a psychiatric disorder, comprising administering to the subject a therapeutically effective amount of a compound of formula I.
  • the present invention provides a method of treating a subject suffering from a drug addiction, such as an alcohol, amphetamine, cocaine, or opiate addiction.
  • halo and halogen are used interchangeably and refer to fluorine, chlorine, bromine or iodine.
  • Ci-C ⁇ alkyl refers to a straight-chain or branched saturated hydrocarbon having from one to six carbon atoms, inclusive. Examples of such groups include, but are not limited to, methyl, ethyl, 1 -propyl, 2-propyl, 1 -butyl, 2-butyl, 2-methyl-2-propyl, 2-methyl-1 -butyl, and n-hexyl.
  • Ci-C ⁇ hydroxyalkyl refers to a CrC ⁇ alkyl group as defined above which is substituted with one hydroxy group.
  • halo(Ci-C 6 )alkyl refers to a d-C 6 alkyl group as defined above which is substituted with up to three halogen atoms, such as trifluoromethyl.
  • Ci-C ⁇ alkoxy refers to a straight-chain or branched saturated alkoxy group having from one to six carbon atoms, inclusive, with the open valency on the oxygen. Examples of such groups include, but are not limited to, methoxy, ethoxy, n- butoxy, 2-methyl-pentoxy and n-hexyloxy.
  • C3-C8 cycloalkyl typically refers to cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl or cyclooctyl.
  • the expression "Ci-C ⁇ alkyl(C 3 -C 8 )cycloalkyl” refers to a C3-C8 cycloalkyl as defined above which is substituted with a straight-chain or branched CrC ⁇ alkyl. Examples of such groups include, but are not limited to, cyclopropylmethyl.
  • heterocycloalkyl refers to a four to eight membered ring containing carbon atoms and up to three N, O or S atoms, provided that the four to eight membered ring does not contain adjacent O or adjacent S atoms.
  • the open valency is on either the heteroatom or carbon atom. Examples of such groups include, but are not limited to, azetidinyl, oxetanyl, piperazinyl, morpholinyl, thiomorpholinyl and [1 ,4]diazepanyl.
  • hydroxyheterocycloalkyl refers to a heterocycloalkyl as defined above which is substituted with one hydroxy group.
  • Ci-C ⁇ alkyl-heterocycloalkyl refers to a heterocycloalkyl as defined above which is substituted with a CrC ⁇ alkyl group.
  • examples of such groups include, but are not limited to, tetrahydropyran-4-yl-methyl and 2-morpholin-4-yl-ethyl.
  • aryl refers to a phenyl ring, optionally substituted with halogen, Ci-C ⁇ alkyl, CrC ⁇ alkoxy or halo(Ci-C6)alkyl as defined above. Examples of such groups include, but are not limited to, phenyl and 4-chlorophenyl.
  • Ci-C6arylalkyl refers to an aryl as defined above which is substituted with a straight-chain or branched C1-C6 alkyl. Examples of such groups include, but are not limited to, benzyl and 4-chlorobenzyl.
  • the present invention further provides certain embodiments of the invention, which are described below.
  • HET is a heteroaromatic group of formula Il containing 2 nitrogen atoms. In another embodiment of the invention, HET is a heteroaromatic group of formula Il containing 3 nitrogen atoms. In yet another embodiment of the invention, HET is a heteroaromatic group of formula Il containing 4 nitrogen atoms.
  • HET is preferably chosen among the following heteroaromatic groups, wherein " * " denotes the attachment point:
  • the heteroaromatic group HET is substituted with one substituent R7 selected from H; C 1 -C 6 alkyl such as methyl; halogen such as chlorine or bromine; cyano; halo(Ci-C 6 )alkyl such as trifluoromethyl; aryl such as phenyl; and d- C ⁇ hydroxyalkyl such as CH 2 CH 2 OH.
  • R7 selected from H; C 1 -C 6 alkyl such as methyl; halogen such as chlorine or bromine; cyano; halo(Ci-C 6 )alkyl such as trifluoromethyl; aryl such as phenyl; and d- C ⁇ hydroxyalkyl such as CH 2 CH 2 OH.
  • HET is substituted with two substituents R7 and R8 individually selected from H; Ci-C ⁇ alkyl such as methyl; halogen such as chlorine or bromine; cyano; halo(Ci-Ce)alkyl such as trifluoromethyl; aryl such as phenyl; and CrC ⁇ hydroxyalkyl such as CH 2 CH 2 OH.
  • HET is substituted with three substituents R7, R8 and R9 individually selected from H; C1-C6 alkyl such as methyl; halogen such as chlorine or bromine; cyano; halo(Ci-C 6 )alkyl such as trifluoromethyl; aryl such as phenyl; and CrC 6 hydroxyalkyl such as CH 2 CH 2 OH.
  • R 7 , R 8 and Rg are all hydrogen. In a different embodiment, at least one of R 7 , Rs and Rg is CrC ⁇ alkyl such as methyl. In a further embodiment, at least one of R 7 , R 8 and Rg is halogen such as chlorine or bromine.
  • HET is imidazo[1 ,2-a]pyrimidine.
  • HET is [1 ,2,4]triazolo[1 ,5-a] pyridine.
  • HET is imidazo[1 ,2-a]pyridine.
  • HET is imidazo [4,5- b]pyrimidine.
  • HET is pyrazolo[1 ,5-a] pyridine.
  • HET is [1 ,2,4]Triazolo[1 ,5-a]pyrimidine.
  • HET is [1 ,2,4]Triazolo[1 ,5-c]pyrimidine. In an eight specific embodiment, HET is [1 ,2,4]Triazolo[1 ,5-a]pyrazine.
  • HET is [1 ,2,4]thazolo[1 ,5-a]pyrimidine. In another specific embodiment, HET is [1 ,2,4]triazolo[1 ,5-a]pyridine-6-carbonitrile. In another specific embodiment, HET is 1-methyl-1 H-benzoimidazole. In another specific embodiment, HET is 1-phenyl-1 H-benzoimidazole. In another specific embodiment, HET is 2-(6-chloro-benzoimidazol-1-yl)-ethanol. In another specific embodiment, HET is 5,7-dimethyl-[1 ,2,4]triazolo[1 ,5-a] pyridine.
  • HET is 5,7- dimethyl-imidazo[1 ,2-a]pyridine. In another specific embodiment, HET is 5-chloro- imidazo[1 ,2-a]pyridine. In another specific embodiment, HET is 5-methyl-imidazo [1 ,2- a]pyridine. In another specific embodiment, HET is 5-trifluoromethyl-imidazo [1 ,2- a]pyridine. In another specific embodiment, HET is 6-Bromo-5,7-dimethyl-[1 ,2,4] triazolo[1 ,5-a] pyridine. In another specific embodiment, HET is 6-bromo-7-methyl- [1 ,2,4]triazolo[1 ,5-a] pyridine.
  • HET is 6-chloro-8- methyl-[1 ,2,4]triazolo[1 ,5-a] pyridine.
  • HET is 6-chloro- imidazo[1 ,2-a]pyridine.
  • HET is 7-methyl- [1 ,2,4]triazolo[1 ,5-a]pyridine.
  • HET is 8-methyl- imidazo[1 ,2-a]pyridine.
  • HET is imidazo[1 ,2-a] pyridine- 7-carbonitrile.
  • HET is 5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5- a]pyrimidine.
  • HET is 5,7-dimethyl-imidazo[1 ,2-a]pyrimidine or [1 ,2,4]Triazolo[1 ,5- c]pyrimidine or [1 ,2,4]Triazolo[1 ,5-a]pyrazine.
  • Ri is H. In another embodiment, Ri is C 1 -C 6 straight or branched chain alkyl. In another embodiment, Ri is C 1 -C 6 hydroxyalkyl. In another embodiment, Ri is Ci-C ⁇ alkyl(C 3 -C 8 )cycloalkyl. In a further embodiment, Ri is d-C ⁇ alkyl-heterocycloalkyl. In another embodiment, Ri is Ci-C ⁇ arylalkyl. In a further embodiment, Ri is CH 2 CN. In a still further embodiment, Ri is CH 2 C(O)NH 2 .
  • Ri is methyl. In another specific embodiment, Ri is ethyl. In another specific embodiment, Ri is 1 -propyl. In another specific embodiment, Ri is 2- propyl. In another specific embodiment, Ri is isobutyl. In another specific embodiment, Ri is hydroxyethyl. In another specific embodiment, Ri is cyclopropylmethyl. In another specific embodiment, Ri is tetrahydropyran-4-yl-methyl. In another specific embodiment, Ri is 2-morpholin-4-yl-ethyl. In another specific embodiment, Ri is benzyl. In another specific embodiment, Ri is 4-chlorobenzyl. In another specific embodiment, Ri is CH 2 CN. In another specific embodiment, Ri is CH 2 C(O)NH 2 .
  • R2, R3, R 4 , R5 and R 6 are all hydrogen. In another embodiment, at least one of R2, R3, R 4 , R5 and R 6 is d-C 6 alkoxy such as methoxy. In a further embodiment of the invention, at least one of R2, R3, R 4 , R5 and R 6 is halogen such as chlorine or fluorine.
  • R 2 is hydrogen. In another embodiment, R 2 is C 1 -C 6 alkoxy such as methoxy. In a further embodiment, R 2 is halogen such as chlorine or fluorine.
  • R 3 is hydrogen. In another embodiment, R 3 is C 1 -C 6 alkoxy such as methoxy. In a further embodiment, R 3 is halogen such as chlorine or fluorine.
  • R 4 is hydrogen. In another embodiment, R 4 is CrC ⁇ alkoxy such as methoxy. In a further embodiment, R 4 is halogen such as chlorine or fluorine.
  • R 5 is hydrogen. In another embodiment, R 5 is C1-C6 alkoxy such as methoxy. In a further embodiment, R5 is halogen such as chlorine or fluorine.
  • R 6 is hydrogen. In another embodiment, R 6 is CrC 6 alkoxy such as methoxy. In a further embodiment, R 6 is halogen such as chlorine or fluorine.
  • HET is 5,7-Dimethyl-imidazo[1 ,2-a]pyrimidine
  • -L- is -S-CH2- or -CH2-S-
  • Ri is selected from hydrogen, methyl, 1 -propyl, isobutyl, cyclopropyl- methyl, benzyl and 2-morpholin-4-yl-ethyl
  • R 2 - R 6 are all hydrogen.
  • HET is selected from 5,7-dimethyl-imidazo [1 ,2- a]pyrimidine, 5,7-dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyridine, 5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5- a]pyrimidine, 5-trifluoromethyl-imidazo [1 ,2-a]pyridine, [1 ,2,4]triazolo[1 ,5-a]pyridine and 6-chloro-8-methyl-[1 ,2,4]triazolo [1 ,5-a]pyridine; -L- is selected from -S-CH2-, -CH2-S- and -CH2CH2-; Ri is selected from hydrogen, methyl, ethyl, 2-propyl, CH 2 CN and tetrahydropyran-4-yl-methyl; and R 2 - R ⁇ are all hydrogen.
  • the compound of formula I is selected among the following specific compounds, in the form of the free base, one or more tautomers thereof or a pharmaceutically acceptable acid addition salt thereof.
  • Table 1 lists compounds of the invention and the corresponding IC50 values determined as described in the section "PDE10A inhibition assay". Each of the compounds constitutes an individual embodiment, of the present invention:
  • the compounds of the present invention have an IC 5 O value of less than 50 nM, such as in the range of 0.2 - 20 nM, particularly in the range of 0.2 - 10 nM, such as in the range of 0.2 - 5 nM or in the range of 0.2 - 1 nM.
  • Selected compounds have been tested for their ability to reverse phencyclidine (PCP) induced hyperactivity. The reversal of the PCP effect is measured as described in the section "Phencyclidine (PCP) induced hyperactivity".
  • compositions also comprises salts of the compounds, typically, pharmaceutically acceptable salts.
  • Such salts include pharmaceutically acceptable acid addition salts.
  • Acid addition salts include salts of inorganic acids as well as organic acids.
  • suitable inorganic acids include hydrochloric, hydrobromic, hydroiodic, phosphoric, sulfuric, sulfamic, nitric acids and the like.
  • suitable organic acids include formic, acetic, trichloroacetic, trifluoroacetic, propionic, benzoic, cinnamic, citric, fumaric, glycolic, itaconic, lactic, methanesulfonic, maleic, malic, malonic, mandelic, oxalic, picric, pyruvic, salicylic, succinic, methane sulfonic, ethanesulfonic, tartaric, ascorbic, pamoic, bismethylene salicylic, ethanedisulfonic, gluconic, citraconic, aspartic, stearic, palmitic, EDTA, glycolic, p- aminobenzoic, glutamic, benzenesulfonic, p-toluen
  • the compounds of this invention may exist in unsolvated as well as in solvated forms with pharmaceutically acceptable solvents such as water, ethanol and the like.
  • pharmaceutically acceptable solvents such as water, ethanol and the like.
  • the solvated forms are considered equivalent to the unsolvated forms for the purposes of this invention.
  • the present invention further provides a pharmaceutical composition comprising a therapeutically effective amount of a compound of formula I and a pharmaceutically acceptable carrier or diluent.
  • the present invention also provides a pharmaceutical composition comprising a therapeutically effective amount of one of the specific compounds disclosed in the Experimental Section herein and a pharmaceutically acceptable carrier or diluent.
  • the compounds of the invention may be administered alone or in combination with pharmaceutically acceptable carriers, diluents or excipients, in either single or multiple doses.
  • pharmaceutical compositions according to the invention may be formulated with pharmaceutically acceptable carriers or diluents as well as any other known adjuvants and excipients in accordance with conventional techniques such as those disclosed in Remington: The Science and Practice of Pharmacy, 19 th Edition, Gennaro, Ed., Mack Publishing Co., Easton, PA, 1995.
  • compositions may be specifically formulated for administration by any suitable route such as oral, rectal, nasal, pulmonary, topical (including buccal and sublingual), transdermal, intracisternal, intraperitoneal, vaginal and parenteral (including subcutaneous, intramuscular, intrathecal, intravenous and intradermal) routes. It will be appreciated that the route will depend on the general condition and age of the subject to be treated, the nature of the condition to be treated and the active ingredient.
  • compositions for oral administration include solid dosage forms such as capsules, tablets, dragees, pills, lozenges, powders and granules. Where appropriate, the compositions may be prepared with coatings such as enteric coatings or they may be formulated so as to provide controlled release of the active ingredient such as sustained or prolonged release according to methods well known in the art.
  • Liquid dosage forms for oral administration include solutions, emulsions, suspensions, syrups and elixirs.
  • compositions for parenteral administration include sterile aqueous and nonaqueous injectable solutions, dispersions, suspensions or emulsions as well as sterile powders to be reconstituted in sterile injectable solutions or dispersions prior to use.
  • Other suitable administration forms include, but are not limited to, suppositories, sprays, ointments, creams, gels, inhalants, dermal patches and implants.
  • Typical oral dosages range from about 0.001 to about 100 mg/kg body weight per day. Typical oral dosages also range from about 0.01 to about 50 mg/kg body weight per day. Typical oral dosages further range from about 0.05 to about 10 mg/kg body weight per day. Oral dosages are usually administered in one or more dosages, typically, one to three dosages per day. The exact dosage will depend upon the frequency and mode of administration, the sex, age, weight and general condition of the subject treated, the nature and severity of the condition treated and any concomitant diseases to be treated and other factors evident to those skilled in the art.
  • a typical unit dosage form for oral administration may contain from about 0.01 to about 1000 mg, from about 0.05 to about 500 mg, or from about 0.5 mg to about 200 mg.
  • parenteral routes such as intravenous, intrathecal, intramuscular and similar administration
  • typical doses are in the order of half the dose employed for oral administration.
  • the present invention also provides a process for making a pharmaceutical composition
  • a process for making a pharmaceutical composition comprising admixing a therapeutically effective amount of a compound of formula I and at least one pharmaceutically acceptable carrier or diluent.
  • the compound utilized in the aforementioned process is one of the specific compounds disclosed in the Experimental Section herein.
  • the compounds of this invention are generally utilized as the free substance or as a pharmaceutically acceptable salt thereof.
  • One example is an acid addition salt of a compound having the utility of a free base.
  • a compound of formula I contains a free base such salts are prepared in a conventional manner by treating a solution or suspension of a free base of formula I with a molar equivalent of a pharmaceutically acceptable acid.
  • suitable organic and inorganic acids are described above.
  • solutions of the compounds of formula I in sterile aqueous solution aqueous propylene glycol, aqueous vitamin E or sesame or peanut oil may be employed.
  • aqueous solutions should be suitably buffered if necessary and the liquid diluent first rendered isotonic with sufficient saline or glucose.
  • the aqueous solutions are particularly suitable for intravenous, intramuscular, subcutaneous and intraperitoneal administration.
  • the compounds of formula I may be readily incorporated into known sterile aqueous media using standard techniques known to those skilled in the art.
  • Suitable pharmaceutical carriers include inert solid diluents or fillers, sterile aqueous solutions and various organic solvents.
  • solid carriers include lactose, terra alba, sucrose, cyclodextrin, talc, gelatin, agar, pectin, acacia, magnesium stearate, stearic acid and lower alkyl ethers of cellulose.
  • liquid carriers include, but are not limited to, syrup, peanut oil, olive oil, phospholipids, fatty acids, fatty acid amines, polyoxyethylene and water.
  • the carrier or diluent may include any sustained release material known in the art, such as glyceryl monostearate or glyceryl distearate, alone or mixed with a wax.
  • sustained release material such as glyceryl monostearate or glyceryl distearate
  • the pharmaceutical compositions formed by combining the compounds of formula I and a pharmaceutically acceptable carrier are then readily administered in a variety of dosage forms suitable for the disclosed routes of administration.
  • the formulations may conveniently be presented in unit dosage form by methods known in the art of pharmacy.
  • Formulations of the present invention suitable for oral administration may be presented as discrete units such as capsules or tablets, each containing a predetermined amount of the active ingredient, and optionally a suitable excipient.
  • the orally available formulations may be in the form of a powder or granules, a solution or suspension in an aqueous or non-aqueous liquid, or an oil-in-water or water-in-oil liquid emulsion.
  • a solid carrier is used for oral administration, the preparation may be tabletted, placed in a hard gelatin capsule in powder or pellet form or it may be in the form of a troche or lozenge.
  • the amount of solid carrier will vary widely but will range from about 25 mg to about 1 g per dosage unit.
  • the preparation may be in the form of a syrup, emulsion, soft gelatin capsule or sterile injectable liquid such as an aqueous or non-aqueous liquid suspension or solution.
  • compositions of the invention may be prepared by conventional methods in the art.
  • tablets may be prepared by mixing the active ingredient with ordinary adjuvants and/or diluents and subsequently compressing the mixture in a conventional tabletting machine prepare tablets.
  • adjuvants or diluents comprise: corn starch, potato starch, talcum, magnesium stearate, gelatin, lactose, gums, and the like. Any other adjuvants or additives usually used for such purposes such as colorings, flavorings, preservatives etc. may be used provided that they are compatible with the active ingredients.
  • the compounds of formula I are PDE10A enzyme inhibitors and as such are useful to treat associated neurological and psychiatric disorders.
  • the invention thus provides a compound of formula I or a pharmaceutically acceptable acid addition salt thereof, as well as a pharmaceutical composition containing such a compound, for use in the treatment of a neurodegenerative disorder, psychiatric disorder or drug addiction in mammals including humans; wherein the neurodegenerative disorder is selected from the group consisting of Alzheimer's disease, multi-infarct dementia, alcoholic dementia or other drug-related dementia, dementia associated with intracranial tumors or cerebral trauma, dementia associated with Huntington's disease or Parkinson's disease, or AIDS-related dementia; delirium; amnestic disorder; post-traumatic stress disorder; mental retardation; a learning disorder, for example reading disorder, mathematics disorder, or a disorder of written expression; attention-deficit/hyperactivity disorder; and age-related cognitive decline; and wherein the psychiatric disorder is selected from the group consisting of schizophrenia, for example of the paranoid, disorganized, catatonic, undifferentiated, or residual type; schizophreniform disorder; schizoaffective disorder, for example
  • the compounds of formula I or pharmaceutically acceptable salts thereof may be used in combination with one or more other drugs in the treatment of diseases or conditions for which the compounds of the present invention have utility, where the combination of the drugs together are safer or more effective than either drug alone. Additionally, the compounds of the present invention may be used in combination with one or more other drugs that treat, prevent, control, ameliorate, or reduce the risk of side effects or toxicity of the compounds of the present invention. Such other drugs may be administered, by a route and in an amount commonly used therefore, contemporaneously or sequentially with the compounds of the present invention.
  • compositions of the present invention include those that contain one or more other active ingredients, in addition to the compounds of the present invention.
  • the combinations may be administered as part of a unit dosage form combination product, or as a kit or treatment protocol wherein one or more additional drugs are administered in separate dosage forms as part of a treatment regimen.
  • the present invention provides a method of treating a mammal, including a human, suffering from a neurodegenerative disorder selected from a cognition disorder or movement disorder, which method comprises administering to the subject a therapeutically effective amount of a compound of formula I.
  • This invention further provides a method of treating a neurodegenerative disorder or condition in a mammal, including a human, which method comprises administering to said mammal an amount of a compound of formula I effective in inhibiting PDE10.
  • This invention also provides a method of treating a subject suffering from a psychiatric disorder, which method comprises administering to the subject a therapeutically effective amount of a compound of formula I.
  • Examples of psychiatric disorders that can be treated according to the present invention include, but are not limited to, schizophrenia, for example of the paranoid, disorganized, catatonic, undifferentiated, or residual type; schizophreniform disorder; schizoaffective disorder, for example of the delusional type or the depressive type; delusional disorder; substance-induced psychotic disorder, for example psychosis induced by alcohol, amphetamine, cannabis, cocaine, hallucinogens, inhalants, opioids, or phencyclidine; personality disorder of the paranoid type; and personality disorder of the schizoid type; and the anxiety disorder is selected from panic disorder; agoraphobia; a specific phobia; social phobia; obsessive- compulsive disorder; post-traumatic stress disorder; acute stress disorder; and generalized anxiety disorder.
  • schizophrenia for example of the paranoid, disorganized, catatonic, undifferentiated, or residual type
  • schizophreniform disorder schizoaffective disorder, for example of the
  • the compounds of formula I or pharmaceutically acceptable salts thereof may advantageously be administered in combination with at least one neuroleptic agent (which may be a typical or an atypical antipsychotic agent) to provide improved treatment of psychiatric disorders such as schizophrenia.
  • the combinations, uses and methods of treatment of the invention may also provide advantages in treatment of patients who fail to respond adequately or who are resistant to other known treatments.
  • the present invention thus provides a method of treating a mammal suffering from a psychiatric disorder, such as schizophrenia, which method comprises administering to the mammal a therapeutically effective amount of a compound of formula I, either alone or as combination therapy together with at least one neuroleptic agent.
  • a psychiatric disorder such as schizophrenia
  • neuroleptic agent refers to drugs, which have the effect on cognition and behaviour of antipsychotic agent drugs that reduce confusion, delusions, hallucinations, and psychomotor agitation in patients with psychoses.
  • neuroleptic agents include, but are not limited to: typical antipsychotic drugs, including phenothiazines, further divided into the aliphatics, piperidines, and piperazines, thioxanthenes (e.g., cisordinol), butyrophenones (e.g., haloperidol), dibenzoxazepines (e.g., loxapine), dihydroindolones (e.g., molindone), diphenylbutylpiperidines (e.g., pimozide), and atypical antipsychotic drugs, including benzisoxazoles (e.g., risperidone), sertindole, olan
  • Particularly preferred neuroleptic agents for use in the invention are sertindole, olanzapine, risperidone, quetiapine, aripiprazole, haloperidol, clozapine, ziprasidone and osanetant.
  • the present invention further provides a method of treating a subject suffering from a cognition disorder, which method comprises administering to the subject a therapeutically effective amount of a compound of formula I.
  • cognition disorders that can be treated according to the present invention include, but are not limited to, Alzheimer's disease, multi-infarct dementia, alcoholic dementia or other drug- related dementia, dementia associated with intracranial tumors or cerebral trauma, dementia associated with Huntington's disease or Parkinson's disease, or AIDS-related dementia; delirium; amnestic disorder; post-traumatic stress disorder; mental retardation; a learning disorder, for example reading disorder, mathematics disorder, or a disorder of written expression; attention-deficit/hyperactivity disorder; and age-related cognitive decline.
  • This invention also provides a method of treating a movement disorder, which method comprises administering to the subject a therapeutically effective amount of a compound of formula I.
  • movement disorders that can be treated according to the present invention include, but are not limited to, Huntington's disease and dyskinesia associated with dopamine agonist therapy.
  • This invention further provides a method of treating a movement disorder selected from Parkinson's disease and restless leg syndrome, which comprises administering to the subject a therapeutically effective amount of a compound of formula I.
  • This invention also provides a method of treating a mood disorder, which method comprises administering to the subject a therapeutically effective amount of a compound of formula I.
  • mood disorders and mood episodes that can be treated according to the present invention include, but are not limited to, major depressive episode of the mild, moderate or severe type, a manic or mixed mood episode, a hypomanic mood episode; a depressive episode with a typical features; a depressive episode with melancholic features; a depressive episode with catatonic features; a mood episode with postpartum onset; post-stroke depression; major depressive disorder; dysthymic disorder; minor depressive disorder; premenstrual dysphoric disorder; post-psychotic depressive disorder of schizophrenia; a major depressive disorder superimposed on a psychotic disorder such as delusional disorder or schizophrenia; a bipolar disorder, for example bipolar I disorder, bipolar Il disorder, and cyclothymic disorder. It is understood that a mood disorder is a psychiatric disorder.
  • This invention further provides a method of treating a drug addiction, for example an alcohol, amphetamine, cocaine, or opiate addiction, in a mammal, including a human, which method comprises administering to said mammal an amount of a compound of formula I effective in treating drug addiction.
  • a drug addiction for example an alcohol, amphetamine, cocaine, or opiate addiction
  • This invention also provides a method of treating a drug addiction, for example an alcohol, amphetamine, cocaine, or opiate addiction, in a mammal, including a human, which method comprises administering to said mammal an amount of a compound of formula I effective in inhibiting PDE10.
  • a drug addiction for example an alcohol, amphetamine, cocaine, or opiate addiction
  • drug addiction means an abnormal desire for a drug and is generally characterized by motivational disturbances such a compulsion to take the desired drug and episodes of intense drug craving.
  • Drug addiction is widely considered a pathological state.
  • the disorder of addiction involves the progression of acute drug use to the development of drug-seeking behavior, the vulnerability to relapse, and the decreased, slowed ability to respond to naturally rewarding stimuli.
  • DSM-IV The Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV) has categorized three stages of addiction: preoccupation/anticipation, binge/intoxication, and withdrawal/negative affect. These stages are characterized, respectively, everywhere by constant cravings and preoccupation with obtaining the substance; using more of the substance than necessary to experience the intoxicating effects; and experiencing tolerance, withdrawal symptoms, and decreased motivation for normal life activities.
  • This invention further provides a method of treating a disorder comprising as a symptom a deficiency in attention and/or cognition in a mammal, including a human, which method comprises administering to said mammal an amount of a compound of formula I effective in treating said disorder.
  • a “neurodegenerative disorder or condition” refers to a disorder or condition that is caused by the dysfunction and/or death of neurons in the central nervous system.
  • the treatment of these disorders and conditions can be facilitated by administration of an agent which prevents the dysfunction or death of neurons at risk in these disorders or conditions and/or enhances the function of damaged or healthy neurons in such a way as to compensate for the loss of function caused by the dysfunction or death of at-risk neurons.
  • neurotrophic agent refers to a substance or agent that has some or all of these properties.
  • neurodegenerative disorders and conditions that can be treated according to the present invention include, but are not limited to, Parkinson's disease; Huntington's disease; dementia, for example Alzheimer's disease, multi-infarct dementia, AIDS-related dementia, and Fronto temperal Dementia; neurodegeneration associated with cerebral trauma; neurodegeneration associated with stroke, neurodegeneration associated with cerebral infarct; hypoglycemia-induced neurodegeneration; neurodegeneration associated with epileptic seizure; neurodegeneration associated with neurotoxin poisoning; and multi-system atrophy.
  • the neurodegenerative disorder or condition involves neurodegeneration of striatal medium spiny neurons in a mammal, including a human.
  • the neurodegenerative disorder or condition is Huntington's disease.
  • the invention provides a method of treating a subject to reduce body fat or body weight, or to treat non-insuline demanding diabetes mellitus (NIDDM), metabolic syndrome, or glucose intolerance, comprising administering to a subject in need thereof a therapeutically effective amount of a compound of formula I.
  • NIDDM non-insuline demanding diabetes mellitus
  • the subject is human, the subject is overweight or obese and the antagonist is administered orally.
  • the method further comprising administering a second therapeutic agent to the subject, preferably an anti- obesity agent, e.g., rimonabant, orlistat, sibutramine, bromocriptine, ephedrine, leptin, pseudoephedrine, or peptide YY3-36, or analogs thereof.
  • an anti- obesity agent e.g., rimonabant, orlistat, sibutramine, bromocriptine, ephedrine, leptin, pseudoephedrine, or peptide
  • metabolic syndrome refers to a constellation of conditions that place people at high risk for coronary artery disease. These conditions include type 2 diabetes, obesity, high blood pressure, and a poor lipid profile with elevated LDL ("bad”) cholesterol, low HDL (“good”) cholesterol, and elevated triglycerides. All of these conditions are associated with high blood insulin levels.
  • LDL low blood lipoprotein
  • good high blood lipoprotein
  • triglycerides elevated triglycerides. All of these conditions are associated with high blood insulin levels.
  • the fundamental defect in the metabolic syndrome is insulin resistance in both adipose tissue and muscle.
  • This reaction is typically carried out in a solvent such as 1-propanol, toluene, DMF, or acetonitrile, optionally in the presence of a carbonate base such as potassium carbonate or a tertiary amine base such as triethylamine or diisopropylethylamine (DIPEA), at a temperature ranging from about 0° C to about 200° C, optionally under pressure in a closed vessel.
  • a solvent such as 1-propanol, toluene, DMF, or acetonitrile
  • a carbonate base such as potassium carbonate
  • a tertiary amine base such as triethylamine or diisopropylethylamine (DIPEA)
  • DIPEA diisopropylethylamine
  • suitable solvents include benzene, chloroform, dioxane, ethyl acetate, 2-propanol and xylene.
  • solvent mixtures
  • Compounds of formula IMa can be prepared as described in WO 96/01826 from the corresponding 1 ,2-diaminopyridines by reaction with thiocarbonyldiimidazole in a suitable solvent, such as chloroform, at a suitable temperature, such as room temperature or +40 0 C.
  • a suitable solvent such as chloroform
  • the requisite 1 ,2-diaminopyridines are readily available from the corresponding commercially available 2-aminopyridines by reaction with a suitable ⁇ /-amination reagent, such as O-(mesitylsulfonyl)hydroxylamine, in a suitable solvent, such as chloroform, at a suitable temperature, such as O 0 C or room temperature, see WO 96/01826.
  • 2-Halomethyl-4-(aryl)-1 H-imidazoles of formula IV can be prepared by halogenation of the corresponding 2-hydroxymethyl-4-(aryl)-1 H-imidazoles using a suitable reagent, e.g. thionyl chloride, phosphorous trichloride, or phosphorous tribromide, optionally using a suitable solvent such as dichloromethane, using methods well known to chemists skilled in the art.
  • the requisite 2-hydroxymethyl-4-(aryl)-1 H-imidazoles can be prepared by methods known in the art (see for example Magdolen, P; Vasella, A. HeIv. Chim. Acta 2005, 88, 2454 - 2469; Song, Z. et al. J. Org. Chem. 1999, 64, 1859-1867).
  • This reaction is typically carried out in a solvent such as 1-propanol, toluene, DMF, or acetonitrile, optionally in the presence of a carbonate base such as potassium carbonate or a tertiary amine base such as triethylamine or diisopropylethylamine (DIPEA), at a temperature ranging from about 0° C to about 200° C, optionally under pressure in a closed vessel.
  • a solvent such as 1-propanol, toluene, DMF, or acetonitrile
  • a carbonate base such as potassium carbonate
  • a tertiary amine base such as triethylamine or diisopropylethylamine (DIPEA)
  • DIPEA diisopropylethylamine
  • suitable solvents include benzene, chloroform, dioxane, ethyl acetate, 2-propanol and xylene.
  • solvent mixtures
  • electrophiles of formula Vl are commercially available, and many others are known in the art, see for example JP 59176277.
  • the electrophile Vl, where X is a leaving group, e.g. Cl, Br, I, methanesulfonyl, 4-toluenesulfonyl, can also be prepared by conversion of the primary alcohol of compounds of formula V to said leaving group by methods known to chemists skilled in the art.
  • Said methods can for example be selected from reacting compounds of formula V with thionyl chloride, phosphorous trichloride, phosphorous tribromide, methanesulfonyl chloride, or 4-toluenesulfonyl chloride optionally in the presence of a suitable solvent, such as dichloromethane or 1 ,2-dichloroethane, and optionally in the presence of a base, such as triethylamine, diisopropylethylamine, or pyridine.
  • electrophiles of formula Vl can be prepared by reacting commercially available aromatic amines of formula VII with 1 ,3- dihaloacetones of formula IX, e.g.
  • alcohols of formula V can be prepared by reacting commercially available aromatic amines of formula VII with a suitable N- amination reagent, such as O-(mesitylsulfonyl)hydroxylamine, in a suitable solvent, such as chloroform, at a suitable temperature, such as 0 0 C or room temperature, see WO 96/01826, to yield compounds of formula VIII.
  • Said compounds of formula VIII can be converted into compounds of formula V by reaction with methyl glycolate followed by reduction of the methyl ester to the requisite alcohol using a suitable reducing agent such as lithium aluminium hydride in a suitable solvent such as diethyl ether or tetrahydrofuran using methods known to chemists skilled in the art.
  • This reaction is typically carried out in a suitable solvent, such as dimethylformamide, dimethylacetamide, or acetonitrile, in the presence of a suitable base such as a carbonate base, e.g. potassium carbonate, or a tertiary amine base, e.g. triethylamine or diisopropylethylamine (DIPEA), at a temperature ranging from about 0° C to about 100 0 C.
  • a suitable solvent such as dimethylformamide, dimethylacetamide, or acetonitrile
  • a suitable base such as a carbonate base, e.g. potassium carbonate, or a tertiary amine base, e.g. triethylamine or diisopropylethylamine (DIPEA)
  • a transition metal catalyst such as palladium metal
  • a hydrogen source such as hydrogen gas, ammonium hydrogen carbonate, or cyclohexadiene.
  • Phosphonium salt of formula XII are readily available by reaction of compounds of formula Vl (see scheme 2 above) with triphenylphosphine by methods known to chemists skilled in the art.
  • Aldehydes of formula XIII are readily available by oxidation of alcohols of formula V (see scheme 2 above) by methods known to chemists skilled in the art, e.g. by reacting alcohols of formula V with a suitable oxidizing agent, such as Dess-Martin periodinane, in a suitable solvent, such as dichloromethane or 1 ,2-dicholorethane.
  • a suitable oxidizing agent such as Dess-Martin periodinane
  • Method C A PE Sciex API 300 instrument equipped with atmospheric pressure photo ionisation and a Waters UPLC system was used.
  • a PE Sciex API 150EX instrument equipped with atmospheric pressure photo ionisation and a Shimadzu LC-8A/SLC-10A LC system was used.
  • 2-Chloromethyl-4-phenyl-1 H-imidazole (by omission of the methylation step) 8811 %% yyiieelldd,, 11 HH NNMMRR ((440000 MMHHzz,, DDMMSSOO--dd 6 ): 58.21 (s, 1 H), 7.96-7.92 (m, 2H), 7.59-7.55 (m, 2H), 7.50-7.47 (m, 1 H), 5.12 (s, 2H).
  • a microwave vial was charged with 2-chloromethyl-5,7-dimethyl-imidazo[1 ,2- a]pyrimidine hydrochloride (500 mg, 2.15 mmol), and 1 ,2-dichloroethane (16 mL) and argon was bubbled through the mixture. To this mixture was added 1 ,8- diazabicyclo[5.4.0]undec-7-ene (0.350 mL, 2.34 mmol) and triphenylphosphine (848 mg, 3.23 mmol). The vial was sealed with a crimp-on cap and the mixture was heated at 140 0 C for 1 hour using a microwave synthesizer.
  • Active PDE10A enzyme is prepared in a number of ways for use in PDE assays (Loughney, K. et al. Gene 1999, 234, 109-117; Fujishige, K. et al. Eur J Biochem. 1999, 266, 1118-1127 and Soderling, S. et al. Proc. Natl. Acad. Sci. 1999, 96, 7071-7076).
  • PDE10A can be expressed as full-length proteins or as truncated proteins, as long as they express the catalytic domain.
  • PDE10A can be prepared in different cell types, for example insect cells or E. coli.
  • catalytically active PDE10A is as follows: The catalytic domain of human PDE10A (amino acids 440-779 from the sequence with accession number NP 006652) is amplified from total human brain total RNA by standard RT-PCR and is cloned into the BamH1 and Xho1 sites of the pET28a vector (Novagen). Expression in coli is performed according to standard protocols. Briefly, the expression plasmids are transformed into the BL21 (DE3) E. coli strain, and 50 mL cultures inoculated with the cells allowed to grow to an OD600 of 0.4- 0.6 before protein expression is induced with 0.5mM IPTG.
  • a PDE10A assay may for example, be performed as follows: The assay is performed in 60 uL samples containing a fixed amount of the relevant PDE enzyme (sufficient to convert 20-25% of the cyclic nucleotide substrate), a buffer (50 mM HEPES7.6; 1OmM MgCI 2 ; 0.02% Tween20), 0.1mg/ml BSA, 225 pCi of 3 H-labelled cyclic nucleotide substrate, tritium labeled cAMP to a final concentration of 5 nM and varying amounts of inhibitors.
  • Reactions are initiated by addition of the cyclic nucleotide substrate, and reactions are allowed to proceed for one hr at room temperature before being terminated through mixing with 15 uL 8 mg/mL yttrium silicate SPA beads (Amersham). The beads are allowed to settle for one hr in the dark before the plates are counted in a Wallac 1450 Microbeta counter. The measured signal can be converted to activity relative to an uninhibited control (100 %) and IC 5 O values can be calculated using the Xlfit extension to EXCEL.
  • the assay was performed in 60 uL assay buffer (50 mM HEPES pH 7.6; 1OmM MgCI 2 ; 0.02% Tween20) containing enough PDEIOA to convert 20-25% of 10 nM 3 H-CAMP and varying amounts of inhibitors. Following a 1 hour incubation the reactions were terminated by addition of 15 uL 8 mg/mL yttrium silicate SPA beads (Amersham). The beads were allowed to settle for one hr in the dark before the plates were counted in a Wallac 1450 Microbeta counter. IC 5 O values were calculated by non linear regression using XLfit (IDBS).
  • mice Male mice (NMRI, Charles River) weighing 20-25g are used. Eight mice are used in each group receiving the test compound (5 mg/kg) plus PCP (2.3 mg/kg) including the parallel control groups receiving the vehicle of the test compound plus PCP or vehicle injections only. The injection volumen is 10 ml/kg. The experiment is made in normal light conditions in an undisturbed room. The test substance is injected per oss 60 min before injection of PCP, which is administered subcutaneous. Immediately after injection of PCP the mice are placed individually in special designed test cage (20 cm x 32 cm). The activity is measured by 5X8 infrared light sources and photocells spaced by 4 cm. The light beams cross the cage 1.8 cm above the bottom of the cage. Recording of a motility count requires interruption of adjacent light beams, thus avoiding counts induced by stationary movements of the mice.
  • Motility is recorded in 5 min intervals for a period of 1 hour.
  • the drug effect is calculated on the total counts during the 1 hour behavioral test period in the following manner:
  • the mean motility induced by vehicle treatment in the absence of PCP is used as baseline.
  • the 100 per cent effect of PCP is accordingly calculated to be total motility counts minus baseline.
  • the response in groups receiving test compound is thus determined by the total motility counts minus baseline, expressed in per cent of the similar result recorded in the parallel PCP control group.
  • the per cent responses are converted to per cent inhibition.

Abstract

This invention is directed to compounds, which are PDE10A enzyme inhibitors. The invention provides a pharmaceutical composition comprising a therapeutically effective, amount of a compound of the invention and a pharmaceutically acceptable carrier. The present invention also provides processes for the preparation of the compounds of formula (I). The present invention further provides a method of treating a subject suffering from a neurodegenerative disorder comprising administering to the subject a therapeutically effective amount of a compound of formula (I). The present invention also provides a method of treating a subject suffering from a drug addiction comprising administering to the subject a therapeutically effective amount of a compound of formula (I). The present invention further provides a method of treating a subject suffering from a psychiatric disorder comprising administering to the subject a therapeutically effective amount of a compound of formula (I).

Description

Novel Phenylimidazole derivatives as PDE10A enzyme inhibitors
Field of the Invention
The present invention provides compounds that are PDE10A enzyme inhibitors, and as such are useful to treat neurodegenerative and psychiatric disorders. Especially, the invention provides compounds that are highly selective for PDE10 over other PDE subtypes. The present invention also provides pharmaceutical compositions comprising compounds of the invention and methods of treating disorders using the compounds of the invention.
Background of the Invention
Throughout this application, various publications are referenced in full. The disclosures of these publications are hereby incorporated by reference into this application to describe more fully the state of the art to which this invention pertains.
The cyclic nucleotides cyclic-adenosine monophosphate (cAMP) and cyclic-guanosine monophosphate (cGMP) function as intracellular second messengers regulating a vast array of processes in neurons. Intracellular cAMP and cGMP are generated by adenyl and guanyl cyclases, and are degraded by cyclic nucleotide phosphodiesterases (PDEs). Intracellular levels of cAMP and cGMP are controlled by intracellular signaling, and stimulation/repression of adenyl and guanyl cyclases in response to GPCR activation is a well characterized way of controlling cyclic nucleotide concentrations (Antoni, F.A. Front. Neuroendocrinal. 2000, 21, 103-132). cAMP and cGMP levels in turn control activity of cAMP- and cGMP-dependent kinases as well as other proteins with cyclic nucleotide response elements, which through subsequent phosphorylation of proteins and other processes regulate key neuronal functions such as synaptic transmission, neuronal differentiation and survival.
There are 21 phosphodiesterase genes that can be divided into 11 gene families. There are ten families of adenylyl cyclases, two of guanylyl cyclases, and eleven of phosphodiesterases. PDEs are a class of intracellular enzymes that regulate levels of cAMP and cGMP via hydrolysis of the cyclic nucleotides into their respective nucleotide monophosphates. Some PDEs degrade cAMP, some cGMP and some both. Most PDEs have a widespread expression and have roles in many tissues, while some are more tissue-specific.
Phosphodieasterase 1OA (PDE10A) is a dual-specificity phosphodiesterase that can convert both cAMP to AMP and cGMP to GMP (Loughney, K. et al. Gene 1999, 234,
109-117; Fujishige, K. et al. Eur. J. Biochem. 1999, 266, 1118-1127 and Soderling, S. et al. Proc. Natl. Acad. Sci. 1999, 96, 7071-7076). PDE10A is primarily expressed in the neurons in the striatum, n. accumbens and in the olfactory tubercle (Kotera, J. et al.
Biochem. Biophys. Res. Comm. 1999, 261, 551-557 and Seeger, T.F. et al. Brain Research, 2003, 985, 113-126).
Mouse PDE10A is the first identified member of the PDE10 family of phosphodiesterases (Fujishige, K. et al. J. Biol. Chem. 1999, 274, 18438-18445 and Loughney, K. et al. Gene 1999, 234, 109-117) and N-terminal splice variants of both the rat and human genes have been identified (Kotera, J. et al. Biochem. Biophys. Res. Comm. 1999, 261, 551-557 and Fujishige, K. et al. Eur. J. Biochem. 1999, 266, 1118- 1127). There is a high degree of homology across species. PDE10A is uniquely localized in mammals relative to other PDE families. mRNA for PDE10 is highly expressed in testis and brain (Fujishige, K. et al. Eur J Biochem. 1999, 266, 1118- 1127; Soderling, S. et al. Proc. Natl. Acad. Sci. 1999, 96, 7071-7076 and Loughney, K. et al. Gene 1999, 234,109-117). These studies indicate that within the brain, PDE10 expression is highest in the striatum (caudate and putamen), n. accumbens and olfactory tubercle. More recently, an analysis has been made of the expression pattern in rodent brain of PDE10A mRNA (Seeger, T.F. et al. Abst. Soc. Neurosci. 2000, 26, 345.10) and PDE10A protein (Menniti, F. S. et al. William Harvey Research Conference 'Phosphodiesterase in Health and Disease', Porto, Portugal, Dec. 5-7, 2001 ).
PDE10A is expressed at high levels by the medium spiny neurons (MSN) of the caudate nucleus, the accumbens nucleus and the corresponding neurons of the olfactory tubercle. These constitute the core of the basal ganglia system. The MSN has a key role in the cortical-basal ganglia-thalamocortical loop, integrating convergent cortical/thalamic input, and sending this integrated information back to the cortex. MSN express two functional classes of neurons: the Di class expressing Di dopamine receptors and the D2 class expressing D2 dopamine receptors. The Di class of neurons is part of the 'direct' striatal output pathway, which broadly functions to facilitate behavioral responses. The D2 class of neurons is part of the 'indirect' striatal output pathway, which functions to suppress behavioral responses that compete with those being facilitated by the 'direct' pathway. These competing pathways act like the brake and accelerator in a car. In the simplest view, the poverty of movement in Parkinson's disease results from over-activity of the 'indirect' pathway, whereas excess movement in disorders such as Huntington's disease represent over-activity of the direct pathway. PDE10A regulation of cAMP and/or cGMP signaling in the dendritic compartment of these neurons may be involved in filtering the cortico/thalamic input into the MSN. Furthermore, PDE10A may be involved in the regulation of GABA release in the substantia nigra and globus pallidus (Seeger, T. F. et al. Brain Research, 2003, 985, 113-126).
Dopamine D2 receptor antagonism is well established in the treatment of schizophrenia. Since the 1950's, dopamine D2 receptor antagonism has been the mainstay in psychosis treatment and all effective antipsychotic drugs antagonise D2 receptors. The effects of D2 are likely to be mediated primarily through neurons in the striatum, n. accumbens and olfactory tubercle, since these areas receive the densest dopaminergic projections and have the strongest expression of D2 receptors (Konradi, C. and Heckers, S. Society of Biological Psychiatry, 2001 , 50, 729-742). Dopamine D2 receptor agonism leads to decrease in cAMP levels in the cells where it is expressed through adenylate cyclase inhibition, and this is a component of D2 signalling (Stoof, J. C; Kebabian J. W. Nature 1981 , 294, 366-368 and Neve, K. A. et al. Journal of Receptors and Signal Transduction 2004, 24, 165-205). Conversely, D2 receptor antagonism effectively increases cAMP levels, and this effect could be mimicked by inhibition of cAMP degrading phosphodiesterases.
Most of the 21 phosphodiesterase genes are widely expressed; therefore inhibition is likely to have side effects. Because PDE10A, in this context, has the desired expression profile with high and relatively specific expression in neurons in striatum, n. accumbens and olfactory tubercle, PDE10A inhibition is likely to have effects similar to D2 receptor antagonism and therefore have antipsychotic effects. While PDE10A inhibition is expected to mimic D2 receptor antagonism in part, it might be expected to have a different profile. The D2 receptor has signalling components besides cAMP (Neve, K. A. et al. Journal of Receptors and Signal Transduction 2004, 24, 165-205), wherefore interference with cAMP through PDE10A inhibition may negatively modulate rather than directly antagonise dopamine signaling through D2 receptors. This may reduce the risk of the extrapyrimidal side effects that are seen with strong D2 antagonism. Conversely, PDE10A inhibition may have some effects not seen with D2 receptor antagonism. PDE10A is also expressed in Di receptors expressing striatal neurons (Seeger, T. F. et al. Brain Research, 2003, 985, 113-126). Since Di receptor agonism leads to stimulation of adenylate cyclase and resulting increase in cAMP levels, PDE10A inhibition is likely to also have effects that mimic Di receptor agonism. Finally, PDE10A inhibition will not only increase cAMP in cells, but might also be expected to increase cGMP levels, since PDE10A is a dual specificity phosphodiesterase. cGMP activates a number of target protein in cells like cAMP and also interacts with the cAMP signalling pathways. In conclusion, PDE10A inhibition is likely to mimic D2 receptor antagonism in part and therefore has antipsychotic effect, but the profile might differ from that observed with classical D2 receptor antagonists.
The PDE10A inhibitor papaverine is shown to be active in several antipsychotic models. Papaverine potentiated the cataleptic effect of the D2 receptor antagonist haloperidol in rats, but did not cause catalepsy on its own (WO 03/093499). Papaverine reduced hyperactivity in rats induced by PCP, while reduction of amphetamine induced hyperactivity was insignificant (WO 03/093499). These models suggest that PDE10A inhibition has the classic antipsychotic potential that would be expected from theoretical considerations. WO 03/093499 further discloses the use of selective PDE10 inhibitors for the treatment of associated neurologic and psychiatric disorders. Furthermore, PDE10A inhibition reverses subchronic PCP-induced deficits in attentional set-shifting in rats (Rodefer et al. Eur. J. Neurosci. 2005, 4, 1070-1076). This model suggests that PDE10A inhibition might alleviate cognitive deficits associated with schizophrenia.
The tissue distribution of PDE10A indicates that PDE10A inhibitors can be used to raise levels of cAMP and/or cGMP within cells that express the PDE10 enzyme, especially neurons that comprise the basal ganglia, and the PDE10A inhibitors of the present invention would therefore be useful in treating a variety of associated neuropsychiatric conditions involving the basal ganglia such as neurological and psychiatric disorders, schizophrenia, bipolar disorder, obsessive compulsive disorder, and the like, and may have the benefit of not possessing unwanted side effects, which are associated with the current therapies on the market.
Furthermore, recent publications (WO 2005/120514, WO 2005012485, Cantin et al, Bioorganic & Medicinal Chemistry Letters 17 (2007) 2869-2873) suggest that PDE10A inhibitors may be useful for treatment of obesity and non-insulin dependent diabetes.
With respect to inhibitors of PDE10A, EP 1250923 discloses the use of selective PDE10 inhibitors in general, and papaverine in particular, for the treatment of certain neurologic and psychiatric disorders.
WO 05/113517 discloses benzodiazepine stereospecific compounds as inhibitors of phosphodiesterase, especially types 2 and 4, and the prevention and treatment of pathologies involving a central and/or peripheral disorder. WO 02/88096 discloses benzodiazepine derivatives and their uses as inhibitors of phosphodiesterase, especially type 4 in the therapeutic field. WO 04/41258 discloses benzodiazepinone derivatives and their uses as inhibitors of phosphodiesterase, especially type 2 in the therapeutic field.
Pyrrolodihydroisoquinolines and variants thereof are disclosed as inhibitors of PDE10 in WO 05/03129 and WO 05/02579. Piperidinyl-substituted quinazolines and isoquinolines that serve as PDE10 inhibitors are disclosed in WO 05/82883. WO 06/11040 discloses substituted quinazoline and isoquinoline compounds that serve as inhibitors of PDE10. US 20050182079 discloses substituted tetrahydroisoquinolinyl derivatives of quinazoline and isoquinoline that serve as effective phosphodiesterase (PDE) inhibitors. In particular, US 20050182079 relates to said compounds, which are selective inhibitors of PDE10. Analogously, US 20060019975 discloses piperidine derivatives of quinazoline and isoquinoline that serve as effective phosphodiesterase (PDE) inhibitors. US 20060019975 also relates to compounds that are selective inhibitors of PDE10. WO 06/028957 discloses cinnoline derivatives as inhibitors of phosphodiesterase type 10 for the treatment of psychiatric and neurological syndromes.
However, these disclosures do not pertain to the compounds of the invention, which are structurally unrelated to any of the known PDE10 inhibitors (Kehler, J. et al. Expert Opin. Ther. Patents 2007, 17, 147-158), and which have now been found by the inventors to be highly active and selective PDE10A enzyme inhibitors.
The compounds 2-(5-Phenyl-1 H-imidazol-2-ylmethylsulfanyl)-1 H-benzoimidazole (CAS Registry no. 348125-42-8) and 2-(5-Phenyl-1 H-imidazol-2-yl-sulfanylmethyl)-1 H- benzoimidazole (CAS Registry no. 296791-07-6) appear in the chemical libraries of Scientific Exchange, Inc. and Zelinsky Institute of Organic Chemistry, respectively, but no pharmacological data appear to have been published. The compounds are both disclaimed from the scope of the present invention.
The compounds of the invention may offer alternatives to current marketed treatments for neurodegenerative and/or psychiatric disorders, which are not efficacious in all patients. Hence, there remains a need for alternative methods of treatment.
Summary of the Invention
The objective of the present invention is to provide compounds that are selective PDE10A enzyme inhibitors.
A further objective of the present invention is to provide compounds which have such activity, and which have improved solubility, metabolic stability and/or bioavailability compared to prior art compounds.
Another objective of the invention is to provide an effective treatment, in particular long- term treatment, of a human patient, without causing the side effects typically associated with current therapies for neurological and psychiatric disorders. Further objectives of the invention will become apparent upon reading the present specification.
Accordingly, in one aspect the present invention relates to compounds of formula I:
Figure imgf000008_0001
wherein HET is a heteroaromatic group of formula Il containing from 2 to 4 nitrogen atoms:
Figure imgf000008_0002
II wherein Y can be N or CH, Z can be N or C, and wherein HET may optionally be substituted with up to three substituents R7, R8 and R9 individually selected from H; Ci-Cε alkyl such as Me; halogen such as chlorine and bromine; cyano; halo(Ci-C6)alkyl such as trifluoromethyl; aryl such as phenyl; alkoxy, preferably Ci-Cβ alkoxy, such as methoxy, dimethoxy, ethoxy, methoxy-ethoxy and ethoxy-methoxy, and C1-C6 hydroxyalkyl such as CH2CH2OH, and wherein * denotes the attachment point,
-L- is a linker selected from -S-CH2-, -CH2-S-, -CH2-CH2- or -CH=CH-,
R1 is selected from H; Ci-C6 alkyl such as methyl, ethyl, 1 -propyl, 2-propyl, isobutyl; d- Cε alkyl(C3-C8)cycloalkyl such as cyclopropylmethyl; Ci-Cβ hydroxyalkyl such as hydroxyethyl; CH2CN; CH2C(O)NH2; Ci-Cε arylalkyl such as benzyl and 4-chlorobenzyl; and C1-C6 alkyl-heterocycloalkyl such as tetrahydropyran-4-yl-methyl and 2-morpholin- 4-yl-ethyl; R2-R6 are each selected independently from H; C1-C6 alkoxy such as methoxy; and halogen such as chlorine or fluorine;
and tautomers and pharmaceutically acceptable acid addition salts thereof, and polymorphic forms thereof, with the proviso that the compound is not 2-(5-Phenyl-1 H- imidazol-2-ylmethylsulfanyl)-1 H-benzoimidazole or 2-(5-Phenyl-1 H-imidazol-2-yl- sulfanylmethyl)-1 H-benzoimidazole
In a particular embodiment, the invention relates to a compound of formula I in the form of a single tautomer or a polymorph.
In separate embodiments of the invention, the compound of formula I is selected among the specific compounds disclosed in the Experimental Section herein.
The invention further provides a compound of formula I, or a pharmaceutically acceptable acid addition salt thereof, for use as a medicament.
In another aspect, the present invention provides a pharmaceutical composition comprising a therapeutically effective amount of a compound of formula I and a pharmaceutically acceptable carrier, diluent or excipient.
The invention further provides the use of a compound of formula I, or a pharmaceutically acceptable acid addition salt thereof, for the preparation of a medicament for the treatment of a neurodegenerative or psychiatric disorder.
Furthermore, in yet another aspect, the present invention provides a method of treating a subject suffering from a neurodegenerative disorder, comprising administering to the subject a therapeutically effective amount of a compound of formula I. In a still further aspect, the present invention provides a method of treating a subject suffering from a psychiatric disorder, comprising administering to the subject a therapeutically effective amount of a compound of formula I. In another embodiment, the present invention provides a method of treating a subject suffering from a drug addiction, such as an alcohol, amphetamine, cocaine, or opiate addiction. Detailed Description of the Invention
Definition of Substitutents
As used in the context of the present invention, the terms "halo" and "halogen" are used interchangeably and refer to fluorine, chlorine, bromine or iodine.
The term "Ci-Cβ alkyl" refers to a straight-chain or branched saturated hydrocarbon having from one to six carbon atoms, inclusive. Examples of such groups include, but are not limited to, methyl, ethyl, 1 -propyl, 2-propyl, 1 -butyl, 2-butyl, 2-methyl-2-propyl, 2-methyl-1 -butyl, and n-hexyl. The expression "Ci-Cβ hydroxyalkyl" refers to a CrCβ alkyl group as defined above which is substituted with one hydroxy group. The term "halo(Ci-C6)alkyl" refers to a d-C6 alkyl group as defined above which is substituted with up to three halogen atoms, such as trifluoromethyl.
The expression "Ci-Cε alkoxy" refers to a straight-chain or branched saturated alkoxy group having from one to six carbon atoms, inclusive, with the open valency on the oxygen. Examples of such groups include, but are not limited to, methoxy, ethoxy, n- butoxy, 2-methyl-pentoxy and n-hexyloxy.
The term "C3-C8 cycloalkyl" typically refers to cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl or cyclooctyl. The expression "Ci-Cε alkyl(C3-C8)cycloalkyl" refers to a C3-C8 cycloalkyl as defined above which is substituted with a straight-chain or branched CrCβ alkyl. Examples of such groups include, but are not limited to, cyclopropylmethyl.
The term "heterocycloalkyl" refers to a four to eight membered ring containing carbon atoms and up to three N, O or S atoms, provided that the four to eight membered ring does not contain adjacent O or adjacent S atoms. The open valency is on either the heteroatom or carbon atom. Examples of such groups include, but are not limited to, azetidinyl, oxetanyl, piperazinyl, morpholinyl, thiomorpholinyl and [1 ,4]diazepanyl. The term "hydroxyheterocycloalkyl" refers to a heterocycloalkyl as defined above which is substituted with one hydroxy group. The term "Ci-Cβ alkyl-heterocycloalkyl" refers to a heterocycloalkyl as defined above which is substituted with a CrCβ alkyl group. Examples of such groups include, but are not limited to, tetrahydropyran-4-yl-methyl and 2-morpholin-4-yl-ethyl.
The term "aryl" refers to a phenyl ring, optionally substituted with halogen, Ci-Cβ alkyl, CrCβ alkoxy or halo(Ci-C6)alkyl as defined above. Examples of such groups include, but are not limited to, phenyl and 4-chlorophenyl.
The term "Ci-C6arylalkyl" refers to an aryl as defined above which is substituted with a straight-chain or branched C1-C6 alkyl. Examples of such groups include, but are not limited to, benzyl and 4-chlorobenzyl.
Additionally, the present invention further provides certain embodiments of the invention, which are described below.
In one embodiment of the invention, HET is a heteroaromatic group of formula Il containing 2 nitrogen atoms. In another embodiment of the invention, HET is a heteroaromatic group of formula Il containing 3 nitrogen atoms. In yet another embodiment of the invention, HET is a heteroaromatic group of formula Il containing 4 nitrogen atoms.
HET is preferably chosen among the following heteroaromatic groups, wherein "*" denotes the attachment point:
Figure imgf000011_0001
Figure imgf000011_0002
In a further embodiment, the heteroaromatic group HET is substituted with one substituent R7 selected from H; C1-C6 alkyl such as methyl; halogen such as chlorine or bromine; cyano; halo(Ci-C6)alkyl such as trifluoromethyl; aryl such as phenyl; and d- Cε hydroxyalkyl such as CH2CH2OH. In another embodiment, HET is substituted with two substituents R7 and R8 individually selected from H; Ci-Cβ alkyl such as methyl; halogen such as chlorine or bromine; cyano; halo(Ci-Ce)alkyl such as trifluoromethyl; aryl such as phenyl; and CrCβ hydroxyalkyl such as CH2CH2OH. In a further embodiment, HET is substituted with three substituents R7, R8 and R9 individually selected from H; C1-C6 alkyl such as methyl; halogen such as chlorine or bromine; cyano; halo(Ci-C6)alkyl such as trifluoromethyl; aryl such as phenyl; and CrC6 hydroxyalkyl such as CH2CH2OH.
In a specific embodiment, R7, R8 and Rg are all hydrogen. In a different embodiment, at least one of R7, Rs and Rg is CrCβ alkyl such as methyl. In a further embodiment, at least one of R7, R8 and Rg is halogen such as chlorine or bromine.
Specific embodiments of the compound for which the HET radical is derived are given below.
In a specific embodiment, HET is imidazo[1 ,2-a]pyrimidine. In a second specific embodiment, HET is [1 ,2,4]triazolo[1 ,5-a] pyridine. In a third specific embodiment, HET is imidazo[1 ,2-a]pyridine. In a fourth specific embodiment, HET is imidazo [4,5- b]pyrimidine. In a fifth specific embodiment, HET is pyrazolo[1 ,5-a] pyridine. In a sixth specific embodiment, HET is [1 ,2,4]Triazolo[1 ,5-a]pyrimidine. In a seventh specific embodiment, HET is [1 ,2,4]Triazolo[1 ,5-c]pyrimidine. In an eight specific embodiment, HET is [1 ,2,4]Triazolo[1 ,5-a]pyrazine.
In another specific embodiment, HET is [1 ,2,4]thazolo[1 ,5-a]pyrimidine. In another specific embodiment, HET is [1 ,2,4]triazolo[1 ,5-a]pyridine-6-carbonitrile. In another specific embodiment, HET is 1-methyl-1 H-benzoimidazole. In another specific embodiment, HET is 1-phenyl-1 H-benzoimidazole. In another specific embodiment, HET is 2-(6-chloro-benzoimidazol-1-yl)-ethanol. In another specific embodiment, HET is 5,7-dimethyl-[1 ,2,4]triazolo[1 ,5-a] pyridine. In another specific embodiment, HET is 5,7- dimethyl-imidazo[1 ,2-a]pyridine. In another specific embodiment, HET is 5-chloro- imidazo[1 ,2-a]pyridine. In another specific embodiment, HET is 5-methyl-imidazo [1 ,2- a]pyridine. In another specific embodiment, HET is 5-trifluoromethyl-imidazo [1 ,2- a]pyridine. In another specific embodiment, HET is 6-Bromo-5,7-dimethyl-[1 ,2,4] triazolo[1 ,5-a] pyridine. In another specific embodiment, HET is 6-bromo-7-methyl- [1 ,2,4]triazolo[1 ,5-a] pyridine. In another specific embodiment, HET is 6-chloro-8- methyl-[1 ,2,4]triazolo[1 ,5-a] pyridine. In another specific embodiment, HET is 6-chloro- imidazo[1 ,2-a]pyridine. In another specific embodiment, HET is 7-methyl- [1 ,2,4]triazolo[1 ,5-a]pyridine. In another specific embodiment, HET is 8-methyl- imidazo[1 ,2-a]pyridine. In another specific embodiment, HET is imidazo[1 ,2-a] pyridine- 7-carbonitrile. In another specific embodiment, HET is 5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5- a]pyrimidine.
Typically, HET is 5,7-dimethyl-imidazo[1 ,2-a]pyrimidine or [1 ,2,4]Triazolo[1 ,5- c]pyrimidine or [1 ,2,4]Triazolo[1 ,5-a]pyrazine.
In another embodiment of the invention, -L- is -S-CH2-. In a further embodiment, -L- is -CH2-S-. In yet another embodiment, -L- is -CH2-CH2-. In a still further embodiment, - L- is -CH=CH-.
In a further embodiment of the invention, Ri is H. In another embodiment, Ri is C1-C6 straight or branched chain alkyl. In another embodiment, Ri is C1-C6 hydroxyalkyl. In another embodiment, Ri is Ci-Cβ alkyl(C3-C8)cycloalkyl. In a further embodiment, Ri is d-Cβ alkyl-heterocycloalkyl. In another embodiment, Ri is Ci-Cβ arylalkyl. In a further embodiment, Ri is CH2CN. In a still further embodiment, Ri is CH2C(O)NH2.
In a specific embodiment, Ri is methyl. In another specific embodiment, Ri is ethyl. In another specific embodiment, Ri is 1 -propyl. In another specific embodiment, Ri is 2- propyl. In another specific embodiment, Ri is isobutyl. In another specific embodiment, Ri is hydroxyethyl. In another specific embodiment, Ri is cyclopropylmethyl. In another specific embodiment, Ri is tetrahydropyran-4-yl-methyl. In another specific embodiment, Ri is 2-morpholin-4-yl-ethyl. In another specific embodiment, Ri is benzyl. In another specific embodiment, Ri is 4-chlorobenzyl. In another specific embodiment, Ri is CH2CN. In another specific embodiment, Ri is CH2C(O)NH2. In one embodiment of the invention, R2, R3, R4, R5 and R6 are all hydrogen. In another embodiment, at least one of R2, R3, R4, R5 and R6 is d-C6 alkoxy such as methoxy. In a further embodiment of the invention, at least one of R2, R3, R4, R5 and R6 is halogen such as chlorine or fluorine.
In one embodiment of the invention, R2 is hydrogen. In another embodiment, R2 is C1-C6 alkoxy such as methoxy. In a further embodiment, R2 is halogen such as chlorine or fluorine.
In one embodiment of the invention, R3 is hydrogen. In another embodiment, R3 is C1-C6 alkoxy such as methoxy. In a further embodiment, R3 is halogen such as chlorine or fluorine.
In one embodiment of the invention, R4 is hydrogen. In another embodiment, R4 is CrCβ alkoxy such as methoxy. In a further embodiment, R4 is halogen such as chlorine or fluorine.
In one embodiment of the invention, R5 is hydrogen. In another embodiment, R5 is C1-C6 alkoxy such as methoxy. In a further embodiment, R5 is halogen such as chlorine or fluorine.
In one embodiment of the invention, R6 is hydrogen. In another embodiment, R6 is CrC6 alkoxy such as methoxy. In a further embodiment, R6 is halogen such as chlorine or fluorine.
It should be understood that the various aspects, embodiments, implementations and features of the invention mentioned herein may be claimed separately, or in any combination, as illustrated by the following non-limiting examples:
In a specific embodiment, HET is 5,7-Dimethyl-imidazo[1 ,2-a]pyrimidine; -L- is -S-CH2- or -CH2-S-; Ri is selected from hydrogen, methyl, 1 -propyl, isobutyl, cyclopropyl- methyl, benzyl and 2-morpholin-4-yl-ethyl; and R2 - R6 are all hydrogen. In another specific embodiment, HET is selected from 5,7-dimethyl-imidazo [1 ,2- a]pyrimidine, 5,7-dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyridine, 5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5- a]pyrimidine, 5-trifluoromethyl-imidazo [1 ,2-a]pyridine, [1 ,2,4]triazolo[1 ,5-a]pyridine and 6-chloro-8-methyl-[1 ,2,4]triazolo [1 ,5-a]pyridine; -L- is selected from -S-CH2-, -CH2-S- and -CH2CH2-; Ri is selected from hydrogen, methyl, ethyl, 2-propyl, CH2CN and tetrahydropyran-4-yl-methyl; and R2 - Rε are all hydrogen.
In separate embodiments of the invention, the compound of formula I is selected among the following specific compounds, in the form of the free base, one or more tautomers thereof or a pharmaceutically acceptable acid addition salt thereof. Table 1 lists compounds of the invention and the corresponding IC50 values determined as described in the section "PDE10A inhibition assay". Each of the compounds constitutes an individual embodiment, of the present invention:
Table 1 : Compounds of the invention and IC50 values
Figure imgf000015_0001
Figure imgf000016_0001
Figure imgf000017_0001
Figure imgf000018_0001
Figure imgf000019_0001
Figure imgf000020_0001
In a particular embodiment of the present invention the compounds of the present invention have an IC5O value of less than 50 nM, such as in the range of 0.2 - 20 nM, particularly in the range of 0.2 - 10 nM, such as in the range of 0.2 - 5 nM or in the range of 0.2 - 1 nM. Selected compounds have been tested for their ability to reverse phencyclidine (PCP) induced hyperactivity. The reversal of the PCP effect is measured as described in the section "Phencyclidine (PCP) induced hyperactivity".
Results of the experiments showed that the tested compounds of the invention are in vivo active compounds that reverse the PCP induced hyperactivity to the % shown in the table.
Table 2: Reversal of PCP induced hyperactivity
Figure imgf000021_0001
Figure imgf000022_0001
Pharmaceutically Acceptable Salts The present invention also comprises salts of the compounds, typically, pharmaceutically acceptable salts. Such salts include pharmaceutically acceptable acid addition salts. Acid addition salts include salts of inorganic acids as well as organic acids.
Representative examples of suitable inorganic acids include hydrochloric, hydrobromic, hydroiodic, phosphoric, sulfuric, sulfamic, nitric acids and the like. Representative examples of suitable organic acids include formic, acetic, trichloroacetic, trifluoroacetic, propionic, benzoic, cinnamic, citric, fumaric, glycolic, itaconic, lactic, methanesulfonic, maleic, malic, malonic, mandelic, oxalic, picric, pyruvic, salicylic, succinic, methane sulfonic, ethanesulfonic, tartaric, ascorbic, pamoic, bismethylene salicylic, ethanedisulfonic, gluconic, citraconic, aspartic, stearic, palmitic, EDTA, glycolic, p- aminobenzoic, glutamic, benzenesulfonic, p-toluenesulfonic acids, theophylline acetic acids, as well as the 8-halotheophyllines, for example 8-bromotheophylline and the like. Further examples of pharmaceutically acceptable inorganic or organic acid addition salts include the pharmaceutically acceptable salts listed in Berge, S. M. et al., J. Pharm. Sci. 1977, 66, 2, the contents of which are hereby incorporated by reference.
Furthermore, the compounds of this invention may exist in unsolvated as well as in solvated forms with pharmaceutically acceptable solvents such as water, ethanol and the like. In general, the solvated forms are considered equivalent to the unsolvated forms for the purposes of this invention. Pharmaceutical compositions
The present invention further provides a pharmaceutical composition comprising a therapeutically effective amount of a compound of formula I and a pharmaceutically acceptable carrier or diluent. The present invention also provides a pharmaceutical composition comprising a therapeutically effective amount of one of the specific compounds disclosed in the Experimental Section herein and a pharmaceutically acceptable carrier or diluent.
The compounds of the invention may be administered alone or in combination with pharmaceutically acceptable carriers, diluents or excipients, in either single or multiple doses. The pharmaceutical compositions according to the invention may be formulated with pharmaceutically acceptable carriers or diluents as well as any other known adjuvants and excipients in accordance with conventional techniques such as those disclosed in Remington: The Science and Practice of Pharmacy, 19th Edition, Gennaro, Ed., Mack Publishing Co., Easton, PA, 1995.
The pharmaceutical compositions may be specifically formulated for administration by any suitable route such as oral, rectal, nasal, pulmonary, topical (including buccal and sublingual), transdermal, intracisternal, intraperitoneal, vaginal and parenteral (including subcutaneous, intramuscular, intrathecal, intravenous and intradermal) routes. It will be appreciated that the route will depend on the general condition and age of the subject to be treated, the nature of the condition to be treated and the active ingredient.
Pharmaceutical compositions for oral administration include solid dosage forms such as capsules, tablets, dragees, pills, lozenges, powders and granules. Where appropriate, the compositions may be prepared with coatings such as enteric coatings or they may be formulated so as to provide controlled release of the active ingredient such as sustained or prolonged release according to methods well known in the art. Liquid dosage forms for oral administration include solutions, emulsions, suspensions, syrups and elixirs.
Pharmaceutical compositions for parenteral administration include sterile aqueous and nonaqueous injectable solutions, dispersions, suspensions or emulsions as well as sterile powders to be reconstituted in sterile injectable solutions or dispersions prior to use. Other suitable administration forms include, but are not limited to, suppositories, sprays, ointments, creams, gels, inhalants, dermal patches and implants.
Typical oral dosages range from about 0.001 to about 100 mg/kg body weight per day. Typical oral dosages also range from about 0.01 to about 50 mg/kg body weight per day. Typical oral dosages further range from about 0.05 to about 10 mg/kg body weight per day. Oral dosages are usually administered in one or more dosages, typically, one to three dosages per day. The exact dosage will depend upon the frequency and mode of administration, the sex, age, weight and general condition of the subject treated, the nature and severity of the condition treated and any concomitant diseases to be treated and other factors evident to those skilled in the art.
The formulations may also be presented in a unit dosage form by methods known to those skilled in the art. For illustrative purposes, a typical unit dosage form for oral administration may contain from about 0.01 to about 1000 mg, from about 0.05 to about 500 mg, or from about 0.5 mg to about 200 mg.
For parenteral routes such as intravenous, intrathecal, intramuscular and similar administration, typical doses are in the order of half the dose employed for oral administration.
The present invention also provides a process for making a pharmaceutical composition comprising admixing a therapeutically effective amount of a compound of formula I and at least one pharmaceutically acceptable carrier or diluent. In an embodiment, of the present invention, the compound utilized in the aforementioned process is one of the specific compounds disclosed in the Experimental Section herein.
The compounds of this invention are generally utilized as the free substance or as a pharmaceutically acceptable salt thereof. One example is an acid addition salt of a compound having the utility of a free base. When a compound of formula I contains a free base such salts are prepared in a conventional manner by treating a solution or suspension of a free base of formula I with a molar equivalent of a pharmaceutically acceptable acid. Representative examples of suitable organic and inorganic acids are described above.
For parenteral administration, solutions of the compounds of formula I in sterile aqueous solution, aqueous propylene glycol, aqueous vitamin E or sesame or peanut oil may be employed. Such aqueous solutions should be suitably buffered if necessary and the liquid diluent first rendered isotonic with sufficient saline or glucose. The aqueous solutions are particularly suitable for intravenous, intramuscular, subcutaneous and intraperitoneal administration. The compounds of formula I may be readily incorporated into known sterile aqueous media using standard techniques known to those skilled in the art.
Suitable pharmaceutical carriers include inert solid diluents or fillers, sterile aqueous solutions and various organic solvents. Examples of solid carriers include lactose, terra alba, sucrose, cyclodextrin, talc, gelatin, agar, pectin, acacia, magnesium stearate, stearic acid and lower alkyl ethers of cellulose. Examples of liquid carriers include, but are not limited to, syrup, peanut oil, olive oil, phospholipids, fatty acids, fatty acid amines, polyoxyethylene and water. Similarly, the carrier or diluent may include any sustained release material known in the art, such as glyceryl monostearate or glyceryl distearate, alone or mixed with a wax. The pharmaceutical compositions formed by combining the compounds of formula I and a pharmaceutically acceptable carrier are then readily administered in a variety of dosage forms suitable for the disclosed routes of administration. The formulations may conveniently be presented in unit dosage form by methods known in the art of pharmacy.
Formulations of the present invention suitable for oral administration may be presented as discrete units such as capsules or tablets, each containing a predetermined amount of the active ingredient, and optionally a suitable excipient. Furthermore, the orally available formulations may be in the form of a powder or granules, a solution or suspension in an aqueous or non-aqueous liquid, or an oil-in-water or water-in-oil liquid emulsion. If a solid carrier is used for oral administration, the preparation may be tabletted, placed in a hard gelatin capsule in powder or pellet form or it may be in the form of a troche or lozenge. The amount of solid carrier will vary widely but will range from about 25 mg to about 1 g per dosage unit. If a liquid carrier is used, the preparation may be in the form of a syrup, emulsion, soft gelatin capsule or sterile injectable liquid such as an aqueous or non-aqueous liquid suspension or solution.
The pharmaceutical compositions of the invention may be prepared by conventional methods in the art. For example, tablets may be prepared by mixing the active ingredient with ordinary adjuvants and/or diluents and subsequently compressing the mixture in a conventional tabletting machine prepare tablets. Examples of adjuvants or diluents comprise: corn starch, potato starch, talcum, magnesium stearate, gelatin, lactose, gums, and the like. Any other adjuvants or additives usually used for such purposes such as colorings, flavorings, preservatives etc. may be used provided that they are compatible with the active ingredients.
Treatment of Disorders
As mentioned above, the compounds of formula I are PDE10A enzyme inhibitors and as such are useful to treat associated neurological and psychiatric disorders.
The invention thus provides a compound of formula I or a pharmaceutically acceptable acid addition salt thereof, as well as a pharmaceutical composition containing such a compound, for use in the treatment of a neurodegenerative disorder, psychiatric disorder or drug addiction in mammals including humans; wherein the neurodegenerative disorder is selected from the group consisting of Alzheimer's disease, multi-infarct dementia, alcoholic dementia or other drug-related dementia, dementia associated with intracranial tumors or cerebral trauma, dementia associated with Huntington's disease or Parkinson's disease, or AIDS-related dementia; delirium; amnestic disorder; post-traumatic stress disorder; mental retardation; a learning disorder, for example reading disorder, mathematics disorder, or a disorder of written expression; attention-deficit/hyperactivity disorder; and age-related cognitive decline; and wherein the psychiatric disorder is selected from the group consisting of schizophrenia, for example of the paranoid, disorganized, catatonic, undifferentiated, or residual type; schizophreniform disorder; schizoaffective disorder, for example of the delusional type or the depressive type; delusional disorder; substance-induced psychotic disorder, for example psychosis induced by alcohol, amphetamine, cannabis, cocaine, hallucinogens, inhalants, opioids, or phencyclidine; personality disorder of the paranoid type; and personality disorder of the schizoid type; and wherein the drug addiction is an alcohol, amphetamine, cocaine, or opiate addiction.
The compounds of formula I or pharmaceutically acceptable salts thereof may be used in combination with one or more other drugs in the treatment of diseases or conditions for which the compounds of the present invention have utility, where the combination of the drugs together are safer or more effective than either drug alone. Additionally, the compounds of the present invention may be used in combination with one or more other drugs that treat, prevent, control, ameliorate, or reduce the risk of side effects or toxicity of the compounds of the present invention. Such other drugs may be administered, by a route and in an amount commonly used therefore, contemporaneously or sequentially with the compounds of the present invention.
Accordingly, the pharmaceutical compositions of the present invention include those that contain one or more other active ingredients, in addition to the compounds of the present invention. The combinations may be administered as part of a unit dosage form combination product, or as a kit or treatment protocol wherein one or more additional drugs are administered in separate dosage forms as part of a treatment regimen.
The present invention provides a method of treating a mammal, including a human, suffering from a neurodegenerative disorder selected from a cognition disorder or movement disorder, which method comprises administering to the subject a therapeutically effective amount of a compound of formula I.
This invention further provides a method of treating a neurodegenerative disorder or condition in a mammal, including a human, which method comprises administering to said mammal an amount of a compound of formula I effective in inhibiting PDE10. This invention also provides a method of treating a subject suffering from a psychiatric disorder, which method comprises administering to the subject a therapeutically effective amount of a compound of formula I. Examples of psychiatric disorders that can be treated according to the present invention include, but are not limited to, schizophrenia, for example of the paranoid, disorganized, catatonic, undifferentiated, or residual type; schizophreniform disorder; schizoaffective disorder, for example of the delusional type or the depressive type; delusional disorder; substance-induced psychotic disorder, for example psychosis induced by alcohol, amphetamine, cannabis, cocaine, hallucinogens, inhalants, opioids, or phencyclidine; personality disorder of the paranoid type; and personality disorder of the schizoid type; and the anxiety disorder is selected from panic disorder; agoraphobia; a specific phobia; social phobia; obsessive- compulsive disorder; post-traumatic stress disorder; acute stress disorder; and generalized anxiety disorder.
It has been found that the compounds of formula I or pharmaceutically acceptable salts thereof may advantageously be administered in combination with at least one neuroleptic agent (which may be a typical or an atypical antipsychotic agent) to provide improved treatment of psychiatric disorders such as schizophrenia. The combinations, uses and methods of treatment of the invention may also provide advantages in treatment of patients who fail to respond adequately or who are resistant to other known treatments.
The present invention thus provides a method of treating a mammal suffering from a psychiatric disorder, such as schizophrenia, which method comprises administering to the mammal a therapeutically effective amount of a compound of formula I, either alone or as combination therapy together with at least one neuroleptic agent.
The term "neuroleptic agent" as used herein refers to drugs, which have the effect on cognition and behaviour of antipsychotic agent drugs that reduce confusion, delusions, hallucinations, and psychomotor agitation in patients with psychoses. Also known as major tranquilizers and antipsychotic drugs, neuroleptic agents include, but are not limited to: typical antipsychotic drugs, including phenothiazines, further divided into the aliphatics, piperidines, and piperazines, thioxanthenes (e.g., cisordinol), butyrophenones (e.g., haloperidol), dibenzoxazepines (e.g., loxapine), dihydroindolones (e.g., molindone), diphenylbutylpiperidines (e.g., pimozide), and atypical antipsychotic drugs, including benzisoxazoles (e.g., risperidone), sertindole, olanzapine, quetiapine, osanetant and ziprasidone.
Particularly preferred neuroleptic agents for use in the invention are sertindole, olanzapine, risperidone, quetiapine, aripiprazole, haloperidol, clozapine, ziprasidone and osanetant.
The present invention further provides a method of treating a subject suffering from a cognition disorder, which method comprises administering to the subject a therapeutically effective amount of a compound of formula I. Examples of cognition disorders that can be treated according to the present invention include, but are not limited to, Alzheimer's disease, multi-infarct dementia, alcoholic dementia or other drug- related dementia, dementia associated with intracranial tumors or cerebral trauma, dementia associated with Huntington's disease or Parkinson's disease, or AIDS-related dementia; delirium; amnestic disorder; post-traumatic stress disorder; mental retardation; a learning disorder, for example reading disorder, mathematics disorder, or a disorder of written expression; attention-deficit/hyperactivity disorder; and age-related cognitive decline.
This invention also provides a method of treating a movement disorder, which method comprises administering to the subject a therapeutically effective amount of a compound of formula I. Examples of movement disorders that can be treated according to the present invention include, but are not limited to, Huntington's disease and dyskinesia associated with dopamine agonist therapy. This invention further provides a method of treating a movement disorder selected from Parkinson's disease and restless leg syndrome, which comprises administering to the subject a therapeutically effective amount of a compound of formula I.
This invention also provides a method of treating a mood disorder, which method comprises administering to the subject a therapeutically effective amount of a compound of formula I. Examples of mood disorders and mood episodes that can be treated according to the present invention include, but are not limited to, major depressive episode of the mild, moderate or severe type, a manic or mixed mood episode, a hypomanic mood episode; a depressive episode with a typical features; a depressive episode with melancholic features; a depressive episode with catatonic features; a mood episode with postpartum onset; post-stroke depression; major depressive disorder; dysthymic disorder; minor depressive disorder; premenstrual dysphoric disorder; post-psychotic depressive disorder of schizophrenia; a major depressive disorder superimposed on a psychotic disorder such as delusional disorder or schizophrenia; a bipolar disorder, for example bipolar I disorder, bipolar Il disorder, and cyclothymic disorder. It is understood that a mood disorder is a psychiatric disorder.
This invention further provides a method of treating a drug addiction, for example an alcohol, amphetamine, cocaine, or opiate addiction, in a mammal, including a human, which method comprises administering to said mammal an amount of a compound of formula I effective in treating drug addiction.
This invention also provides a method of treating a drug addiction, for example an alcohol, amphetamine, cocaine, or opiate addiction, in a mammal, including a human, which method comprises administering to said mammal an amount of a compound of formula I effective in inhibiting PDE10.
The term "drug addiction", as used herein, means an abnormal desire for a drug and is generally characterized by motivational disturbances such a compulsion to take the desired drug and episodes of intense drug craving.
Drug addiction is widely considered a pathological state. The disorder of addiction involves the progression of acute drug use to the development of drug-seeking behavior, the vulnerability to relapse, and the decreased, slowed ability to respond to naturally rewarding stimuli. For example, The Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV) has categorized three stages of addiction: preoccupation/anticipation, binge/intoxication, and withdrawal/negative affect. These stages are characterized, respectively, everywhere by constant cravings and preoccupation with obtaining the substance; using more of the substance than necessary to experience the intoxicating effects; and experiencing tolerance, withdrawal symptoms, and decreased motivation for normal life activities.
This invention further provides a method of treating a disorder comprising as a symptom a deficiency in attention and/or cognition in a mammal, including a human, which method comprises administering to said mammal an amount of a compound of formula I effective in treating said disorder.
Other disorders that can be treated according to the present invention are obsessive/compulsive disorders, Tourette's syndrome and other tic disorders.
As used herein, and unless otherwise indicated, a "neurodegenerative disorder or condition" refers to a disorder or condition that is caused by the dysfunction and/or death of neurons in the central nervous system. The treatment of these disorders and conditions can be facilitated by administration of an agent which prevents the dysfunction or death of neurons at risk in these disorders or conditions and/or enhances the function of damaged or healthy neurons in such a way as to compensate for the loss of function caused by the dysfunction or death of at-risk neurons. The term "neurotrophic agent" as used herein refers to a substance or agent that has some or all of these properties.
Examples of neurodegenerative disorders and conditions that can be treated according to the present invention include, but are not limited to, Parkinson's disease; Huntington's disease; dementia, for example Alzheimer's disease, multi-infarct dementia, AIDS-related dementia, and Fronto temperal Dementia; neurodegeneration associated with cerebral trauma; neurodegeneration associated with stroke, neurodegeneration associated with cerebral infarct; hypoglycemia-induced neurodegeneration; neurodegeneration associated with epileptic seizure; neurodegeneration associated with neurotoxin poisoning; and multi-system atrophy. In one embodiment of the present invention, the neurodegenerative disorder or condition involves neurodegeneration of striatal medium spiny neurons in a mammal, including a human.
In a further embodiment of the present invention, the neurodegenerative disorder or condition is Huntington's disease.
In another embodiment, the invention provides a method of treating a subject to reduce body fat or body weight, or to treat non-insuline demanding diabetes mellitus (NIDDM), metabolic syndrome, or glucose intolerance, comprising administering to a subject in need thereof a therapeutically effective amount of a compound of formula I. In preferred embodiments, the subject is human, the subject is overweight or obese and the antagonist is administered orally. In another preferred embodiment, the method further comprising administering a second therapeutic agent to the subject, preferably an anti- obesity agent, e.g., rimonabant, orlistat, sibutramine, bromocriptine, ephedrine, leptin, pseudoephedrine, or peptide YY3-36, or analogs thereof.
The term "metabolic syndrome" as used herein refers to a constellation of conditions that place people at high risk for coronary artery disease. These conditions include type 2 diabetes, obesity, high blood pressure, and a poor lipid profile with elevated LDL ("bad") cholesterol, low HDL ("good") cholesterol, and elevated triglycerides. All of these conditions are associated with high blood insulin levels. The fundamental defect in the metabolic syndrome is insulin resistance in both adipose tissue and muscle.
All references, including publications, patent applications and patents, cited herein are hereby incorporated by reference in their entirety and to the same extent as if each reference were individually and specifically indicated to be incorporated by reference and were set forth in its entirety (to the maximum extent permitted by law).
Headings and sub-headings are used herein for convenience only, and should not be construed as limiting the invention in any way. The use of any and all examples, or exemplary language (including "for instance", "for example", "e.g.", and "as such") in the present specification is intended merely to better illuminate the invention, and does not pose a limitation on the scope of invention unless otherwise indicated.
The citation and incorporation of patent documents herein is done for convenience only, and does not reflect any view of the validity, patentability and/or enforceability of such patent documents.
The present invention includes all modifications and equivalents of the subject-matter recited in the claims appended hereto, as permitted by applicable law.
Experimental Section
Preparation of the compounds of the invention
Figure imgf000033_0001
Compounds of the general formula I of the invention may be prepared as described in the following reaction schemes. Unless otherwise indicated, in the reaction schemes and discussion that follow, HET, R1-R9, -L-, Z and Y are as defined above.
Compounds of formula I, wherein -L- is -S-CH2-, can be prepared by the coupling of a nucleophile of formula III or IMa with an electrophile of formula IV, where X is a leaving group, e.g. Cl, Br, I, methanesulfonyl, 4-toluenesulfonyl, as shown in scheme 1. In the reaction between IMa and IV, alkylation of the sulfur atom of IMa with IV and ring closure to form the triazole ring both take place under the same reaction conditions in a one-pot procedure.
Figure imgf000034_0001
IHa IV
Scheme 1.
This reaction is typically carried out in a solvent such as 1-propanol, toluene, DMF, or acetonitrile, optionally in the presence of a carbonate base such as potassium carbonate or a tertiary amine base such as triethylamine or diisopropylethylamine (DIPEA), at a temperature ranging from about 0° C to about 200° C, optionally under pressure in a closed vessel. Other suitable solvents include benzene, chloroform, dioxane, ethyl acetate, 2-propanol and xylene. Alternatively, solvent mixtures such as toluene/2-propanol can be used.
Compounds of formula III are either commercially available or can be prepared as described in the literature, see for example Brown et al. Aust. J. Chem. 1978, 31, 397- 404; Yutilov et al. Khim. Geter. Soedin. 1988, 799-804; Wilde et al. Bioorg. Med. Chem. Lett. 1995, 5, 167-172; Kidwai et al. J. Korean Chem. Soc. 2005, 49, 288-291. Compounds of formula IMa can be prepared as described in WO 96/01826 from the corresponding 1 ,2-diaminopyridines by reaction with thiocarbonyldiimidazole in a suitable solvent, such as chloroform, at a suitable temperature, such as room temperature or +40 0C. The requisite 1 ,2-diaminopyridines are readily available from the corresponding commercially available 2-aminopyridines by reaction with a suitable Λ/-amination reagent, such as O-(mesitylsulfonyl)hydroxylamine, in a suitable solvent, such as chloroform, at a suitable temperature, such as O 0C or room temperature, see WO 96/01826.
2-Halomethyl-4-(aryl)-1 H-imidazoles of formula IV can be prepared by halogenation of the corresponding 2-hydroxymethyl-4-(aryl)-1 H-imidazoles using a suitable reagent, e.g. thionyl chloride, phosphorous trichloride, or phosphorous tribromide, optionally using a suitable solvent such as dichloromethane, using methods well known to chemists skilled in the art. The requisite 2-hydroxymethyl-4-(aryl)-1 H-imidazoles can be prepared by methods known in the art (see for example Magdolen, P; Vasella, A. HeIv. Chim. Acta 2005, 88, 2454 - 2469; Song, Z. et al. J. Org. Chem. 1999, 64, 1859-1867).
Compounds of formula I, wherein -L- is -CH2-S -, can be prepared by the coupling of a nucleophile of formula X with an electrophile of formula Vl as shown in scheme 2.
Figure imgf000035_0001
VIII VII
Scheme 2.
This reaction is typically carried out in a solvent such as 1-propanol, toluene, DMF, or acetonitrile, optionally in the presence of a carbonate base such as potassium carbonate or a tertiary amine base such as triethylamine or diisopropylethylamine (DIPEA), at a temperature ranging from about 0° C to about 200° C, optionally under pressure in a closed vessel. Other suitable solvents include benzene, chloroform, dioxane, ethyl acetate, 2-propanol and xylene. Alternatively, solvent mixtures such as toluene/2-propanol can be used.
Some electrophiles of formula Vl are commercially available, and many others are known in the art, see for example JP 59176277. The electrophile Vl, where X is a leaving group, e.g. Cl, Br, I, methanesulfonyl, 4-toluenesulfonyl, can also be prepared by conversion of the primary alcohol of compounds of formula V to said leaving group by methods known to chemists skilled in the art. Said methods can for example be selected from reacting compounds of formula V with thionyl chloride, phosphorous trichloride, phosphorous tribromide, methanesulfonyl chloride, or 4-toluenesulfonyl chloride optionally in the presence of a suitable solvent, such as dichloromethane or 1 ,2-dichloroethane, and optionally in the presence of a base, such as triethylamine, diisopropylethylamine, or pyridine. Alternatively, electrophiles of formula Vl can be prepared by reacting commercially available aromatic amines of formula VII with 1 ,3- dihaloacetones of formula IX, e.g. 1 ,3-dichloroacetone, in a suitable solvent, such as 1 ,2-dimethoxyethane or ethanol, at a suitable temperature, such as room temperature or reflux. Some electrophiles of formula V are commercially available, and many others are known in the art, see for example Tsuchiya, T.; Sashida, H. J. Chem. Soc, Chem. Commun. 1980, 1109-1110; Tsuchiya, T.; Sashida, H; Konoshita, A. Chem. Pharm. Bull. 1983, 31, 4568-4572. Alternatively, alcohols of formula V can be prepared by reacting commercially available aromatic amines of formula VII with a suitable N- amination reagent, such as O-(mesitylsulfonyl)hydroxylamine, in a suitable solvent, such as chloroform, at a suitable temperature, such as 0 0C or room temperature, see WO 96/01826, to yield compounds of formula VIII. Said compounds of formula VIII can be converted into compounds of formula V by reaction with methyl glycolate followed by reduction of the methyl ester to the requisite alcohol using a suitable reducing agent such as lithium aluminium hydride in a suitable solvent such as diethyl ether or tetrahydrofuran using methods known to chemists skilled in the art.
Compounds of formula X are either commercially available or can be prepared as described in the literature, see e.g. Kjellin, G; Sandstrδm, J. Acta Chem. Scand. 1969, 23, 2879-2887; Laufer, S. A. et al. Synthesis 2008, 253-266. Compounds of formula I, wherein R1 is not hydrogen, can be prepared by the alkylation of a compounds of formula I, wherein R1 is hydrogen, with an alkyl halide of formula Xl as shown in scheme 3.
Figure imgf000037_0001
I XI I
(where Rl = H)
Scheme 3.
This reaction is typically carried out in a suitable solvent, such as dimethylformamide, dimethylacetamide, or acetonitrile, in the presence of a suitable base such as a carbonate base, e.g. potassium carbonate, or a tertiary amine base, e.g. triethylamine or diisopropylethylamine (DIPEA), at a temperature ranging from about 0° C to about 1000 C.
Compounds of formula I, wherein -L- is -CH=CH- or -CH2-CH2- can be prepared by the reaction sequence shown in scheme 4.
Figure imgf000038_0001
Base
Figure imgf000038_0002
(where -L- = -CH2-CH2- (where -L- = -CH=CH- and HET is as shown) and HET is as shown)
Scheme 4.
Specifically, compounds of formula I, wherein -L- is -CH2-CH2- can be prepared by reduction of an alkene of formula I, wherein -L- is -CH=CH-, by hydrogenation using a transition metal catalyst, such as palladium metal, together with a hydrogen source, such as hydrogen gas, ammonium hydrogen carbonate, or cyclohexadiene. Said alkenes of formula I, wherein -L- is -CH=CH- can be prepared by the Wittig reaction between a phosphonium salt of formula XII and an aldehyde of formula XIII in a suitable solvent, such as tetrahydrofuran, in the presence of a suitable base, such as 1 ,8- diazabicyclo[5.4.0]undec-7-ene. Phosphonium salt of formula XII are readily available by reaction of compounds of formula Vl (see scheme 2 above) with triphenylphosphine by methods known to chemists skilled in the art. Aldehydes of formula XIII are readily available by oxidation of alcohols of formula V (see scheme 2 above) by methods known to chemists skilled in the art, e.g. by reacting alcohols of formula V with a suitable oxidizing agent, such as Dess-Martin periodinane, in a suitable solvent, such as dichloromethane or 1 ,2-dicholorethane.
The invention disclosed herein is further illustrated by the following non-limiting examples. General Methods
Analytical LC-MS data were obtained using one of the following methods.
Method A: A PE Sciex API 150EX instrument equipped with atmospheric pressure photo ionisation and a Shimadzu LC-8A/SLC-10A LC system was used. Column: 4.6 x 30 mm Waters Symmetry C18 column with 3.5 μm particle size; Column temperature: 60 0C; Solvent system: A = water/trifluoroacetic acid (100:0.05) and B = water/ acetonitrile/trifluoroacetic acid (5:95:0.035); Method: Linear gradient elution with A:B = 90:10 to 0:100 in 2.4 minutes and with a flow rate of 3.3 mL/min.
Method B:
An Agilent 1100 LCMS system with a G1946C or a G1946A mass detector was used. Column: 2.0 x 50 mm YMC ODS-AQ with 5 μm particle size; Column temperature: 50 0C; Solvent system: A = water/trifluoroacetic acid (99.9:0.1 ) and B = acetonitrile/trifluoroacetic acid (99.95:0.05); Method: Linear gradient elution with A:B = 95:5 to 0:100 in 3.5 minutes and with a flow rate of 0.8 mL/min.
Method C: A PE Sciex API 300 instrument equipped with atmospheric pressure photo ionisation and a Waters UPLC system was used. Column: Acquity UPLC BEH Ciβ 1.7 μm, 2.1 x 50 mm (Waters); Column temperature: 60 0C; Solvent system: A = water/trifluoroacetic acid (100:0.05) and B = water/acetonitrile/trifluoroacetic acid (5:95:0.035); Method: Linear gradient elution with A:B = 90:10 to 0:100 in 1.0 minutes and with a flow rate of 1.2 mL/min.
Method D:
An Agilent 1100 LCMS system with a G1946C or a G1946A mass detector was used. Column: 2.0 x 50 mm YMC ODS-AQ with 5 μm particle size; Column temperature: 50 0C; Solvent system: A = water/trifluoroacetic acid (99.9:0.1 ) and B = acetonitrile/trifluoroacetic acid (99.95:0.05); Method: Linear gradient elution with A:B = 90:10 to 0:100 in 3.4 minutes and with a flow rate of 0.8 mL/min. Method E:
A PE Sciex API 150EX instrument equipped with atmospheric pressure photo ionisation and a Shimadzu LC-8A/SLC-10A LC system was used. Column: 4.6 x 30 mm Waters Symmetry C18 column with 3.5 μm particle size; Column temperature: 60 0C; Solvent system: A = water/trifluoroacetic acid (99.95:0.05) and B = methanol/trifluoroacetic acid (99.965:0.035); Method: Linear gradient elution with A:B = 83:17 to 0:100 in 2.4 minutes and with a flow rate of 3.0 mL/min.
Preparative LC-MS-purification was performed on a PE Sciex API 150EX instrument with atmospheric pressure chemical ionization. Column: 50 X 20 mm YMC ODS-A with 5 μm particle size; Method: Linear gradient elution with A:B = 80:20 to 0:100 in 7 minutes and with a flow rate of 22.7 mL/minute. Fraction collection was performed by split-flow MS detection.
1H NMR spectra were recorded at 500.13 MHz on a Bruker Avance AV500 instrument or at 250.13 MHz on a Bruker Avance DPX250 instrument. TMS was used as internal reference standard. Chemical shift values are expressed in ppm. The following abbreviations are used for multiplicity of NMR signals: s = singlet, d = doublet, t = triplet, q = quartet, qui = quintet, h = heptet, dd = double doublet, dt = double triplet, dq = double quartet, tt = triplet of triplets, m = multiplet, br s = broad singlet and br = broad signal.
Abbreviations are in accordance with to the ACS Style Guide: "The ACS Styleguide - A manual for authors and editors" Janet S. Dodd, Ed. 1997, ISBN: 0841234620
Preparation of intermediates
2-Chloromethyl-1 -methyl-4-phenyl-1 H-imidazole
Figure imgf000041_0001
4 5 6
An adaptation of the method described by Song et al., J. Org. Chem. 1999, 64, 1859 was used. To a round-bottom flask equipped with a nitrogen inlet, a gas outlet to a bleach scrubber and a temperature probe was charged DMSO (113 ml_) and acetophenone 1 (1O g, 83.2 mmol). The solution was heated to 60 0C, and aqueous HBr was added slowly via an addition funnel while maintaining the reaction temperature between 60 0C and 68 0C. A nitrogen sweep was employed to remove the dimethyl sulfide as it was formed. Once the HBr addition was complete, the internal temperature was maintained at 65 0C with external heating until the reaction was complete. The reaction was quenched by pouring the reaction mixture into water, extracted by ethyl acetate and afforded 2,2-dihydroxy-1-phenyl-ethanone 2. The reaction was monitored by TLC.
To a round-bottom flask charged with methyl 2-hydroxy-2-methoxyacetate (2.14 g, 25.9 mmol) and ammonium acetate (4.108 g, 52 mmol) in methanol (30 ml_), acetic acid (30 ml_) was added dropwise, followed by the addition of a solution of 2,2-dihydroxy -1- phenylethanone 2 (2 g, 13 mmol) in methanol with stirring. After 1.5 hours, the reaction mixture was concentrated in vacuo and was then mixed with 0.5 N hydrochloric acid. The solution was washed with ethyl acetate. The aqueous layer was basified with 5N sodium hydroxide to pH = 9 and extracted with ethyl acetate 3 times. The combined organic layer was dried over Na2SO4. The solution was concentrated to dryness to give the compound 4-Phenyl-1 H-imidazole-2- carboxylic acid methyl ester 3. To a solution of compound 3 (1.0 g, 5 mmol) in DMF (20 ml_) was added iodomethane (4 ml_, 7.5 mmol) and K2COs (LO g, 7.5 mmol), and the mixture was stirred at 6O0C for 1 hour until TLC (petroleum ether / EtOAc = 5/1 ) showed that compound 3 was consumed completely. The reaction mixture was diluted with brine (20 ml_) and was extracted with ethyl acetate (2 x 10 ml_). The combined organic layers were dried over anhydrous Na2SO4, filtered, and concentrated under vacuum to afford compound 4 (0.83 g, 78%). 1H NMR (400 MHz, CDCI3): 57.81-7.78 (m, 2H), 7.39-7.35 (m, 2H), 7.32 (s, 1 H), 7.29-7.25 (m, 1 H), 4.05 (s, 3H), 3.97 (s, 3H).
To a solution of compound 4 (0.8 g, 3.7 mmol) in THF (8 ml_) was added LiAIH4 (0.21 g, 5.5 mmol) at -50C under N2. The mixture was stirred at -1O0C for 2 hours and was quenched by aqueous NH4CI solution at O0C until pH reached 6. The resulting mixture was extracted with EtOAc (3 * 20 mL), and the combined organic layers were washed with brine (30 mL), dried over Na2SO4, filtered, and concentrated under reduced pressure to afford compound 5 (0.5 g, 75%). 1H NMR (400 MHz, DMSO-d6): 57.69- 7.67 (m, 2H), 7.53 (s, 1 H), 7.30 (t, J = 7.6 Hz, 2H), 7.15-7.12 (m, 1 H), 5.30 (t, J = 5.6 Hz, 1 H), 4.48 (d, J = 5.6 Hz, 2H), 3.65 (s, 3H).
To a solution of (1-Methyl-4-phenyl-1 H-imidazol-2-yl)-methanol 5 (0.2 g, 0.097 mmol) was added SOCI2 (0.14 g, 0.121 mmol), and the mixture was stirred at room temperature overnight. The mixture was evaporated to afford 2-Chloromethyl-1-methyl- 4-phenyl-1 H-imidazole 6, which was used without purification. 1H NMR (400 MHz, DMSO-de): 58.24 (s, 1 H), 7.87 (d, J = 6.8 Hz, 2H), 7.54-7.49 (m, 2H), 7.43-7.41 (m, 1 H), 5.24 (s, 2H), 3.89 (s, 3H).
The following intermediates were prepared in a similar way:
2-Chloromethyl-1 -ethyl-4-phenyl-1 H-imidazole
95% yield, 1H NMR (400 MHz, DMSO-d6): 58.36 (s, 1 H), 7.92-7.89 (m, 2H), 7.51-7.47 (m, 2H), 7.43-7.39 (m, 1 H), 5.26 (s, 2H), 4.24 (q, J = 7.2Hz, 2H), 1.46 (t, J = 7.2Hz, 3H). 2-Chloromethyl-1 -isopropyl-4-phenyl-1 H-imidazole
100% yield, 1H NMR (400 MHz, DMSO-d6): 58.54 (s, 1 H), 7.93 (d, J = 7.6 Hz, 2H),
7.52-7.29 (m, 3H), 5.28 (s, 2H), 4.84-4.75 (m, 1 H), 1.50 (d, J = 6.8Hz, 6H).
2-chloromethyl-4-(2-fluorophenyl)-1 -methyl-1 H-imidazole
80% yield, 1H NMR (400 MHz, DMSO-d6): 58.06-8.02 (m, 1 H), 7.91 (d, J = 3.2Hz, 1 H), 7.41-7.38 (m, 1 H), 7.34-7.29 (m, 2H), 5.12 (s, 2H), 3.84 (s, 3H).
2-chloromethyl-4-(3-fluorophenyl)-1 -methyl-1 H-imidazole 89% yield, 1H NMR (400 MHz, Methanol-^): 58.07(s, 1 H), 7.58-7.51 (m, 3H), 7.27-7.23 (m, 1 H), 5.11 (s, 2H), 4.01 (s, 3H).
2-chloromethyl-4-(4-fluorophenyl)-1 -methyl-1 H-imidazole
74% yield, 1HNMR (400 MHz, DMSO-d6): 58.19 (s, 1 H), 7.94-7.91 (m, 2H), 7.37-7.33 (m, 2H), 5.20 (s, 2H), 3.86 (s, 3H).
2-chloromethyl-4-(2-chlorophenyl)-1 -methyl-1 H-imidazole
74% yield, 1H NMR (400 MHz, DMSO-d6): 58.11 (s, 1 H), 7.89 (dd, J = 7.6 Hz, 1.6Hz, 1 H), 7.59 (d, J = 7.6Hz, 1 H), 7.48-7.38 (m, 2H), 5.13 (s, 2H), 3.87 (s, 3H).
2-chloromethyl-4-(3-chlorophenyl)-1 -methyl-1 H-imidazole
99% yield, 1H NMR (400 MHz, DMSO-d6): 58.30 (s, 1 H), 8.00-7.99 (m, 1 H), 7.84 (m,
1 H), 7.52-7.43 (m, 2H), 5.20 (s, 2H), 3.86 (s, 3H).
2-chloromethyl-4-(4-chlorophenyl)-1 -methyl-1 H-imidazole
80% yield, 1H NMR (400 MHz, Methanol-^): 58.00 (s, 1 H), 7.71 (d, J = 8.4 Hz, 2H), 7.56 (d, J = 8.4 Hz, 2H), 5.10 (s, 2H), 4.01 (s, 3H).
2-chloromethyl-4-(2-methoxyphenyl)-1 -methyl-1 H-imidazole 93% yield, 1H NMR (400 MHz, DMSO-d6): 58.12 (s, 1 H), 7.98 (dd, J = 8.0Hz, 1.6Hz, 1 H), 7.45-7.40 (m, 1H), 7.20 (d, J = 8.0Hz, 1 H), 7.13-7.06 (m, 1 H), 5.27 (s, 2H), 3.93 (s, 3H), 3.90 (s, 3H). 2-chloromethyl-4-(3-methoxyphenyl)-1 -methyl-1 H-imidazole 9900%% yyiieelldd,, 11HH NNMMRR ((330000 MMHHzz,, MMeetthhaannooll--^^)):: 5588..0055 ((ss,, 11 HH), 7.55-7.44 (m, 1 H), 7.32- 7.24 (m, 2H), 7.14-7.06 (m, 1 H), 5.12 (s, 2H), 4.03 (s, 3H), 3.90 (s, 3H).
2-chloromethyl-4-(4-methoxyphenyl)-1 -methyl-1 H-imidazole
97% yield, 1H NMR (400 MHz, DMSO-d6): 58.10 (s, 1 H), 7.79 (d, J = 8.8Hz, 2H), 7.02
(d, J = 8.8Hz, 2H), 5.20 (s, 2H), 3.83 (s, 3H), 3.75(s, 3H).
2-Chloromethyl-4-phenyl-1 H-imidazole (by omission of the methylation step) 8811 %% yyiieelldd,, 11HH NNMMRR ((440000 MMHHzz,, DDMMSSOO--dd6): 58.21 (s, 1 H), 7.96-7.92 (m, 2H), 7.59-7.55 (m, 2H), 7.50-7.47 (m, 1 H), 5.12 (s, 2H).
1 -Methyl-4-phenyl-1 H-imidazole-2-carbaldehyde
Figure imgf000044_0001
5 To a solution of (1-Methyl-4-phenyl-1 H-imidazol-2-yl)-methanol 5 (50.0 mg, 0.266 mmol) in 1 ,2-dichloroethane (4.0 ml_) under Ar was added Dess-Martin periodinane (124 mg, 0.292 mmol), and the mixture was stirred at room temperature for 2 hours. Saturated NaHCθ3 solution was added, the organic layer was separated and the aqueous layer was extracted with 1 ,2-dichloroethane. The combined organic layers were dried over Na2SO4, volatiles were evaporated and the residue was purified by silica gel chromatography on a FlashMaster system (gradient elution; 0-100 % ethylacetate in heptane) to afford the title compound as a white solid (39.1 mg, 79%). 1H NMR (500 MHz, DMSO-d6): 59.76 (s, 1 H), 8.11 (s, 1 H), 7.84 (d, J = 7.7 Hz, 2H), 7.42 (t, J = 7.6 Hz, 2H), 7.30 (t, J = 7.4 Hz, 1 H), 3.99 (s, 3H). lmidazole-1 -carbothioic acid (2-imino-4,6-dimethyl-2H-pyridin-1 -yl)-amide
Figure imgf000045_0001
CHCU , 00C
Figure imgf000045_0002
10
An adaptation of the method described in WO 96/01826 was used. To a solution of ethyl O-mesitylsulfonylacetohydroxamate 7 (1.7 g, 6.0 mmol) in 1 ,4-dioxane (10 ml_) cooled in an ice bath (freezes at 8-9 0C) was added 70% perchloric acid (7.5 ml_) dropwise over 15 minutes, maintaining internal temperature below 15 0C. The mixture was then diluted with ice water (100 ml_) to precipitate the product O- (mesitylsulfonyl)hydroxylamine 8 which was filtered off, washed thoroughly with water, and immediately dissolved in chloroform (10 ml_) while still wet (CAUTION! 8 is explosive when dry!). The organic layer was separated and was passed through a plug of Na2SO4 in a fritted syringe to remove water. The so obtained solution of O- (mesitylsulfonyl)hydroxylamine 8 was added dropwise to a solution of 2-amino-4,6- dimethylpyridine 9 (0.611 g, 5.00 mmol) in chloroform (10 ml_) cooled in an ice bath. The mixture was then warmed to room temperature and was stirred for 2 hours to effect conversion to intermediate 10. Into the reaction mixture was then added 1 ,1 '- thiocarbonyldiimidazole 11 (1.16 g, 6.5 mmol) and the resulting mixture was stirred at 40 0C overnight. Volatiles were evaporated and the residue was chromatographed on silica gel (gradient elution with heptane:ethyl acetate 100:0 -> 0:100) to yield imidazole- 1-carbothioic acid (2-imino-4,6-dimethyl-2H-pyridin-1-yl)-amide 12 as an off-white solid (0.50 g, 40%) containing a minor amount of residual imidazole. 1H NMR (500 MHz, DMSO-de): δ7.88 (s, 1 H), 7.64 (s, 1 H), 7.42 (br s, 2H), 6.93 (s, 1 H), 6.69 (s, 1 H), 6.67 (s, 1 H), 2.28 (s, 3H), 2.27 (s, 3H).
The following intermediates were prepared analogously, except that they were used for the preparation of final compounds without prior purification or characterization:
lmidazole-1-carbothioic acid (5-bromo-2-imino-4-methyl-2H-pyridin-1-yl)-amide lmidazole-1-carbothioic acid (5-bromo-2-imino-4,6-dimethyl-2H-pyridin-1-yl)-amide lmidazole-1-carbothioic acid (5-chloro-2-imino-3-methyl-2H-pyridin-1-yl)-amide lmidazole-1-carbothioic acid (5-cyano-2-imino-2H-pyridin-1-yl)-amide
1 -Methyl-4-phenyl-1 ,3-dihydro-imidazole-2-thione
Figure imgf000046_0001
An adaptation of the method reported by Kjellin and Sandstrδm, Acta Chem. Scand. 1969, 23, 2879-2887 was used. A mixture of 2-methylamino-1-phenyl-ethanone hydrochloride (0.754 g, 4.06 mmol) (see e.g. Hyde et al. J. Am. Chem. Soc. 1928, 50, 2287-2292; Shang et al. Chem. Eur. J. 2007, 13, 7780-7784) and potassium thiocyanate (0.434 g, 4.46 mmol) in acetic acid (12 ml_) was heated at 140 0C for 10 minutes using a microwave synthesizer. Dilution with water (50 ml_) and cooling in an ice bath caused the product to precipitate. It was collected by filtration, washed with water, and vacuum dried to yield the title compound (0.365 g, 47%) pure as an off-white solid. 1H NMR (500 MHz, DMSO-d6): 512.66 (br s, 1 H), 7.65 (d, J = 7.6 Hz, 2H), 7.60 (s, 1 H), 7.39 (t, J = 7.8 Hz, 2H), 7.27 (t, J = 7.4 Hz, 1 H), 3.49 (s, 3H).
The following intermediate was prepared analogously:
4-Phenyl-1 ,3-dihydro-imidazole-2-thione
80% yield, 1H NMR (500 MHz, DMSO-d6): 512.53 (br s, 1 H), 12.15 (br s, 1 H), 7.69-7.65 (m, 2H), 7.41-7.35 (m, 3H) 7.27 (t, J = 7.4 Hz, 1 H).
4-Phenyl-1 H-imidazole-2-carbaldehyde
Figure imgf000047_0001
A solution of phenylglyoxal monohydrate (102 g, 0.67 mol) and glyoxal dimethyl acetal (60% solution in water, 232 ml_, 1.54 mol) in methanol (1.1 L) was treated with a solution of ammonium acetate 8202 g, 2.61 mol) in methanol (1.1 L) and the resulting solution stirred at RT for 16 h. The volatiles were removed in vacuo and the residue slurried in 2N HCI solution (1.1 L) and heated at 800C for 30 min. The cooled solution was extracted with EtOAc (200 mL) and the separated aqueous layer was basified to pH 9 with 9N NaOH solution. The solids were filtered, washed with water and dried in vacuo to yield the title compound (97.2 g, 84%) as a light brown solid. LC-MS: m/z = 173.0 (MH+), tR = 0.66 min, method C
1 -(2-Hydroxypropyl)-4-Phenyl-1 H-imidazole-2-carbaldehyde
Figure imgf000047_0002
In a closed vessel a slurry of 4-phenyl-1 H-imidazole-2-carbaldehyde (200 mg, 1.16 mmol) and sodium carbonate (60 mg, 0.6 mmol) in ethanol (4 mL) was treated with propylene oxide (170 μL, 2.4 mmol) and heated at 1000C for 3 h. The cooled solution was filtered and the solids washed with DCM. The volatiles were removed in vacuo to yield the crude title compound which was used without further purification (250 mg, 63%). LC-MS: m/z = 231.5 (MH+), tR = 0.41 min, method A The following intermediates were prepared analogously, except that they were used for the preparation of final compounds without prior purification or characterization:
(S)-1 -(2-Hydroxypropyl)-4-Phenyl-1 H-imidazole-2-carbaldehyde
(R)-1 -(2-Hydroxypropyl)-4-Phenyl-1 H-imidazole-2-carbaldehyde
1 -(2-Hydroxy-2-methyl-propyl)-4-phenyl-1 H-imidazole-2-carbaldehyde from 1 -chloro-2- methyl-2-propanol.
2-Chloromethyl-5,7-dimethyl-imidazo[1,2-a]pyrimidine
Figure imgf000048_0001
A solution of 2-amino-4,6-dimethylpyrimidine (2.46 g, 20.0 mmol) and 1 ,3-dichloro-2- propanone (2.67 g, 21.0 mmol) in 1 ,2-dimethoxyethane (20 ml_) was stirred at 45 0C overnight. A precipitate formed, and this was collected by filtration, and was then refluxed with ethanol (15 ml_) for 2 hours. After cooling to room temperature, the product precipitated as white needles which were collected by filtration and vacuum dried to yield the title compound pure as its hydrochloride salt (883 mg, 19%). 1H NMR (500 MHz, DMSO-de): δ7.84 (s, 1 H), 6.88 (s, 1 H), 4.84 (s, 2H), 2.60 (s, 3H), 2.49 (s, 3H).
The following intermediate was prepared analogously, but with a 90 0C reaction temperature for the first step:
2-Chloromethyl-imidazo[1 ,2-a]pyrimidine hydrochloride
62% yield, LC-MS: m/z = 168.2 (MH+), tR = 0.13 min, method A. 2-Chloromethyl-5,7-dimethyl-[1,2,4]triazolo[1,5-a]pyrimidine
Figure imgf000049_0001
4,6-Dimethyl-pyrimidin-2-ylamine
Figure imgf000049_0002
1-Amino-4,6-dimethyl-1 H-pyrimidin-2 -ylidene-ammonium 2,4,6-Trimethyl-benzenesulfonate
Figure imgf000049_0003
2-Chloromethyl-5,7-dimethyl-[1 ,2,4] triazolo[1 ,5-a]pyrimidine
To a solution of 4,6-Dimethyl-pyrimidin-2-ylamine (25 g, 200 mmol) in 400 ml_ of CH2CI2 was added dropwise a solution of hydroxylamine-2,4,6-Trimethyl- benzenesulfonate (105 g, 488 mmol) in 300 ml_ Of CH2CI2 at O0C, and the mixture was stirred at OoC for 1 hand filtered. The solid collected was washed with CH2CI2 (100 ml_) to give 1-Amino-4,6-dimethyl-1 H-pyrimidin-2-ylidene-ammonium 2,4,6-Trimethyl- benzenesulfonate (40 g, yield:62%).
A mixture of 1-Amino-4,6-dimethyl-1 H-pyrimidin-2-ylidene-ammonium 2,4,6-Trimethyl- benzenesulfonate (40 g, 0.1 mol) and NaOH (10 g, 0.2 mol) in 500 ml_ of EtOH was stirred at 50~60°C for 1 hour. After chloroacetic acid methyl ester (16.6 g, 0.15 mol) was added, the resultant mixture was stirred at reflux for 4 hours. After being concentrated under reduce pressure, the residue was diluted with water (1000 ml_) and extracted with CH2CI2 (300 ml_χ3). The combined organic layers were washed with brine (200 ml_), dried over Na2SO4, filtered, and concentrated under vacuum. The residue was purified by column chromatography on silica gel (petroleum ether/EtOAc = 2/1 ) to give 2 g of 2-Chloromethyl-5,7-dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidine in 9% yield. 1H NMR (300 MHz, DMSO-d6): 58.55 (s, 1 H), 6.25 (s, 2H), 4.05 (s, 3H), 3.95 (s, 3H); LC-MS (MH+): m/z = 196.9, tR (min, method A) =0.52
The following intermediates were prepared analogously:
7-Chloro-2-chloromethyl-5,8-dimethyl-[1 ,2,4]triazolo[1 ,5-c]pyrimidine from 6-Chloro- 2,5-dimethyl-pyrimidine-4-ylamine prepared as described by Henze et al. J. Org. Chem 1952, 17, 1320-1327. 3.2% yield, LC-MS: m/z = 231.5 (MH+), tR = 1.13 min, method E
2-Chloromethyl-5,8-dimethyl-[1 ,2,4]-triazolo[1 ,5-a]pyrazine from 2-amino-3,6- dimethylpyrazine. 60% yield, 1H NMR (500 MHz, CDCI3): 57.91 (s,1 H), 4.87 (s, 2H), 2.91 (s, 3H), 2.74 (s, 3H), LC-MS: m/z = 196.9 (MH+), tR = 0.64 min, method A
2-Chloromethyl-5,8-dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyridine from 6-Chloro-5-ethyl-2- methyl-pyrimidin-4-ylamine. 21 % yield, LC-MS: m/z = 245.0 (MH+), tR = 0.72 min, method A
2-Chloromethyl-8-methoxy-5-methyl-[1 ,2,4]triazolo[1 ,5-a]pyridine from 3-Methoxy-6- methyl-pyridin-2-ylamine
2-Chloromethyl-imidazo[1,2-a]pyridine
Figure imgf000050_0001
The method of Vanelle et al. Tetrahedron 1991 , 47, 5173-5184 was used. To a solution of 1 ,3-dichloro-2-propanone (2.69 g, 21.2 mmol) in 1 ,2-dimethoxyethane (5 mL) was added 2-aminopyridine and the mixture was stirred at room temperature for 2 hours. During this time a thick precipitate formed, and this was collected by filtration. The precipitate was refluxed in absolute ethanol for 2 hours after which volatiles were removed by evaporation. The residue was dissolved in water (30 mL) and solid NaHCO3 was added to neutralize the mixture. A white precipitate formed, and this was collected by filtration, washed with water and vacuum dried to yield the title compound pure as a cream white solid (1.43 g, 42%). 1H NMR (500 MHz, CDCI3): 58.08 (d, J = 6.7 Hz, 1 H), 7.62 (s, 1 H), 7.58 (d, J = 9.0 Hz, 1 H), 7.17-7.22 (m, 1 H), 6.80 (t, J = 6.8 Hz, 1 H), 4.78 (s, 2H).
The following intermediate was prepared analogously:
2-Chloromethyl-8-methyl-imidazo[1 ,2-a]pyridine
53% yield, 1H NMR (500 MHz, CDCI3): 57.95 (d, J = 6.9 Hz, 1 H), 7.61 (s, 1 H), 6.97 (dt,
J = 7.0 Hz, 1.1 Hz, 1 H), 6.70 (t, J = 6.8 Hz, 1 H), 4.80 (s, 2H), 2.60 (s, 3H).
2-Chloromethyl-5,7-dimethyl-[1,2,4]triazolo[1,5-a]pyridine
Figure imgf000051_0001
To a solution of 0.79 g of sodium hydroxide in ethanol (20 ml_) was added 2-imino-4,6- dimethyl-2H-pyridin-1-ylamine (1.7 g, 0.012 mol; obtained by HPLC purification of intermediate 10). After being stirred at 50 - 60 0C for 1 hour, methyl glycolate (1.4 g, 0.016 mol) was added, and the resulting mixture was stirred at reflux for 6 hours. After removal of the solvent under reduced pressure, the residue was purified by column chromatography on silica gel (ethyl acetate) to afford (5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5- a]pyridin-2-yl)-methanol (0.2 g, 10%); 1H NMR (300 MHz, DMSO-d6): 57.39 (s, 1 H), 6.87 (s, 1 H), 5.38 (t, J = 6.3 Hz, 1 H), 4.59 (d, J = 6.3 Hz, 2H), 2.64 (s, 3H), 2.38 (s, 3H). A mixture of this compound (31 mg, 0.175 mmol) and SOCI2 (10 ml_) in dry CH2CI2 (10 ml_) was stirred at room temperature for 2 hours. The solvent and excess SOCI2 was evaporated under vacuum to yield the title compound as a crude product, which was used for the preparation of final compounds without purification or characterization.
The following compounds are known in the art:
2-Chloromethyl-1-phenyl-1 H-benzoimidazole (JP 59176277).
1-Methyl-1 ,3-dihydro-benzoimidazole-2-thione (Wilde et al. Bioorg. Med. Chem. Lett.
1995, 5, 167-172).
1-Phenyl-1 ,3-dihydro-benzoimidazole-2-thione (Kidwai et al. J. Korean Chem. Soc. 2005, 49, 288-291). [1 ,2,4]Triazolo[1 ,5-a]pyrimidine-2-thione (Brown et al. Aust. J. Chem. 1978, 31, 397- 404).
1 ,3-Dihydro-imidazo[4,5-b]pyridine-2-thione (Yutilov et al. Khim. Geter. Soedin. 1988, 799-804). Pyrazolo[1 ,5-a]pyridin-2-yl-methanol (Tsuchiya, T.; Sashida, H. J. Chem. Soc, Chem. Commun. 1980, 1109-1110; Tsuchiya, T.; Sashida, H; Konoshita, A. Chem. Pharm. Bull. 1983, 37, 4568-4572).
Preparation of the compounds of the invention
Example 1
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)[1 ,2,4] triazolo[1 ,5-a]pyridine
Figure imgf000052_0001
An adaptation of the method described in WO 96/01826 was used, lmidazole-1- carbothioic acid (2-imino-2H-pyridin-1-yl)-amide (200 mg, 1.37 mmol) and 2- chloromethyl-1-methyl-4-phenyl-1 H-imidazole 6 (300 mg, 1.46 mmol) were dissolved in
1-propanol (25 ml_) and the mixture was heated to reflux for 2 hours. The solvent was removed under reduced pressure and the residue dissolved in dichloromethane. The solution was washed with water and the organic layer was dried over Na2SO4 and concentrated. The residue was purified by chromatography on silica gel to afford the title compound (273 mg, 62 %) as a yellow solid. LC-MS: mlz = 322.1 (MH+), tR = 2.29 min, method B.
The following compounds of the invention were prepared analogously:
7-Methyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine (from imidazole-1 -carbothioic acid (2-imino-4-methyl-2H-pyridin-1-yl)-amide (see WO 96/01826) and 2-chloromethyl-1-methyl-4-phenyl-1 H-imidazole 6). LC-MS: m/z = 336.5 (MH+), tR = 0.71 min, method A. 5,7-Dimethyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine (from imidazole-1-carbothioic acid (2-imino-4,6-dimethyl-2H-pyridin-1-yl)- amide 12 and 2-chloromethyl-1-methyl-4-phenyl-1 H-imidazole 6). LC-MS: m/z = 350.3 (MH+), tR = 0.79 min, method A.
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1 ,2,4]triazolo[1 ,5-a]pyrimidine (from [1 ,2,4]triazolo[1 ,5-a]pyrimidine-2-thiol (commercially available; see also Brown et al. Aust. J. Chem. 1978, 31, 397-404) and 2-chloromethyl-1-methyl-4-phenyl-1 H- imidazole 6). LC-MS: m/z = 323.1 (MH+), tR = 2.07 min, method B.
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-1 H-imidazo[4,5-b]pyridine (from 1 ,3-dihydro-2H-lmidazo[4,5-b]pyridine-2-thione (commercially available; see also Yutilov et al. Khim. Geter. Soedin. 1988, 799-804) and 2-chloromethyl-1-methyl-4- phenyl-1 H-imidazole 6). LC-MS: m/z = 322.1 (MH+), tR = 2.01 min, method B.
Example 2 2-(4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1,2,4]triazolo[1,5-a]pyridine
Figure imgf000053_0001
A solution of imidazole-1-carbothioic acid (2-imino-2H-pyridin-1-yl)-amide (18 mg, 0.080 mmol) in DMF (0.5 mL) was added to 2-chloromethyl-5-phenyl-1 H-imidazole (23 mg, 0.12 mmol) and the mixture was heated at 100 0C overnight. Volatiles were evaporated and the residue was purified by preparative LC-MS to yield the title compound. LC-MS: m/z = 308.2 (MH+), tR = 0.67 min, method A.
The following compounds of the invention were prepared analogously:
2-[4-(3-Chloro-phenyl)-1 -methyl-1 H-imidazol-2-ylmethylsulfanyl]-[1 ,2,4]triazolo[1 ,5-a] pyridine. LC-MS: m/z = 356.4 (MH+), tR = 0.76 min, method A. 2-(1 -Ethyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1 ,2,4]triazolo[1 ,5-a]pyridine. LC- MS: m/z = 336.4 (MH+), tR = 0.69 min, method A. 2-(1 -lsopropyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1 ,2,4]triazolo[1 ,5-a]pyridine.
LC-MS: m/z = 350.3 (MH+), tR = 0.77 min, method A.
2-[4-(4-Fluoro-phenyl)-1-methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine. LC-MS: mlz = 368.2 (MH+), tR = 0.83 min, method A. 2-[4-(3-Fluoro-phenyl)-1 -methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine. LC-MS: m/z = 368.3 (MH+), tR = 0.84 min, method A.
2-[4-(3-Chloro-phenyl)-1 -methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine. LC-MS: m/z = 384.3 (MH+), tR = 0.93 min, method A.
2-(1 -Ethyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-5,7-dimethyl-[1 ,2,4]triazolo[1 ,5- a]pyridine. LC-MS: mlz = 364.4 (MH+), tR = 0.88 min, method A.
5,7-Dimethyl-2-(4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1 ,2,4]triazolo[1 ,5-a]pyridine.
LC-MS: m/z = 336.4 (MH+), tR = 0.78 min, method A.
2-[4-(4-Fluoro-phenyl)-1 -methyl-1 H-imidazol-2-ylmethylsulfanyl]-[1 ,2,4]triazolo[1 ,5- a]pyridine. LC-MS: mlz = 340.3 (MH+), tR = 0.65 min, method A. 2-[4-(3-Fluoro-phenyl)-1 -methyl-1 H-imidazol-2-ylmethylsulfanyl]-[1 ,2,4]triazolo[1 ,5- a]pyridine. LC-MS: mlz = 340.3 (MH+), tR = 0.65 min, method A.
2-[4-(4-Chloro-phenyl)-1 -methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine. LC-MS: m/z = 384.4 (MH+), tR = 0.94 min, method A.
6-Bromo-7-methyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)[1 ,2,4]- triazolo[1 ,5-a]pyridine. LC-MS: mlz = 414.1 (MH+), tR = 0.89 min, method A.
6-Bromo-5,7-dimethyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)[1 ,2,4]- triazolo[1 ,5-a]pyridine. LC-MS: mlz = 428.0 (MH+), tR = 1.00 min, method A.
6-Chloro-8-methyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1 ,2,4]- triazolo[1 ,5-a]pyridine. LC-MS: mlz = 370.1 (MH+), tR = 0.87 min, method A. 2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1 ,2,4]triazolo[1 ,5-a]pyridine-6- carbonitrile. LC-MS: m/z = 347.0 (MH+), tR = 0.64 min, method A.
2-[4-(2-Chloro-phenyl)-1 -methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine. LC-MS: m/z = 384.3 (MH+), tR = 0.87 min, method A.
2-[4-(2-Fluoro-phenyl)-1 -methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl- [1 ,2,4]triazolo[1 ,5-a]pyridine. LC-MS: mlz = 368.4 (MH+), tR = 0.83 min, method A.
2-[4-(4-Methoxy-phenyl)-1 -methyl-1 H-imidazol-2-ylmethylsulfanyl]-5, 7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine. LC-MS: m/z = 380.6 (MH+), tR = 0.84 min, method A. 2-[4-(3-Methoxy-phenyl)-1-methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl- [1 ,2,4]triazolo[1 ,5-a]pyridine. LC-MS: m/z = 380.4 (MH+), tR = 0.85 min, method A. 2-[4-(2-Methoxy-phenyl)-1-methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl- [1 ,2,4]triazolo[1 ,5-a]pyridine. LC-MS: m/z = 380.5 (MH+), tR = 0.86 min, method A.
Example 3
1 -Methyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-1 H- benzoimidazole
Figure imgf000055_0001
To a solution of 1-methyl-1 ,3-dihydro-benzoimidazole-2-thione (28 mg, 0.18 mmol) in DMF (1.6 ml_) was added DIPEA (80 μl_, 0.44 mmol) and 2-chloromethyl-1 -methyl-4- phenyl-1 H-imidazole (40 mg, 0.19 mmol). The mixture was heated at 90 0C for 10 minutes using a microwave synthesizer. Volatiles were evaporated and the residue was purified by preparative LC-MS to yield the title compound. LC-MS: m/z = 335.3 (MH+), tR = 0.51 min, method C.
The following compound of the invention was prepared analogously:
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-1 -phenyl-1 H-benzoimidazole. LC-
MS: m/z = 396.9 (MH+), tR = 0.65 min, method C.
2-[4-(3-Methoxy-phenyl)-1-methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl- [1 ,2,4]triazolo[1 ,5-a]pyrimidine, LC-MS (MH+): m/z = 381.5, tR (min, method A) =0.68
5,7-Dimethyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1 ,2,4]triazolo[1 ,5- a]pyrimidine, LC-MS (MH+): m/z = 351.4, fR (min, method A) =0.62 Example 4
Preparation of 5-Methyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)- imidazo[1 ,2-a]pyridine
Figure imgf000056_0001
1 ,3-dichloroacetone (0.01 mL, 0.11 mmol) was added dropwise to a solution of 6- trifluoromethyl-pyridin-2-ylamine (0.016 g, 0.10 mmol) in 1 ,2-dimethoxyethane (1.0 mL), and the mixture was left to stir at room temperature for 2 h. The solvent was removed in vacuo and the resulting residue re-dissolved in ethanol (1.0 mL). The reaction mixture was subsequently heated under reflux for 2 h, and the solvent was removed under reduced pressure. DIPEA (0.05 mL, 0.25 mmol) and 1-methyl-4 phenyl-1 ,3-dihydro- imidazole-2-thione (0.017 g, 0.09 mmol) were sequentially added to a solution of the crude product in DMF (1.0 mL). The reaction mixture was then heated at 60 °C for 2 h after which LC-MS showed complete consumption of the starting materials. The solvent was removed under reduced pressure and the crude product purified using preparative LC-MS to yield the title compound. LC-MS: mlz = 389.1 (MH+), fR = 0.52 min, method C.
The following compounds of the invention were prepared analogously:
5-Methyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2-a] pyridine.
LC-MS: m/z = 335.4 (MH+), tR = 0.54 min, method A.
5,7-Dimethyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2-a] pyridine. LC-MS: mlz = 349.1 (MH+), fR = 0.61 min, method A.
5-Chloro-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2-a] pyridine. LC-MS: mlz = 355.4 (MH+), tR = 0.69 min, method A.
6-Chloro-8-methyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2- a]pyridine. LC-MS: m/z = 369.2 (MH+), fR = 0.76 min, method A.
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2-a]pyridine-7- carbonitrile. LC-MS: m/z = 346.2 (MH+), fR = 0.66 min, method A. Example 5
5,7-dimethyl-2-((1 -methyl-4-phenyl-1 H-imidazol-2-ylthio)methyl)imidazo[1 ,2- a]pyrimidine
Figure imgf000057_0001
A mixture of 2-chloromethyl-5,7-dimethyl-imidazo[1 ,2-a]pyrimidine (1.55 g, 0.8 mmol), 1-methyl-4-phenyl-1 ,3-dihydro-imidazole-2-thione (1.5 g, 0.8 mmol) and K2Cθ3 (3.31 g, 2.4 mmol) in dry DMF (20 ml_) was stirred under N2 at room temperature overnight. After removal of the solvent under vacuum, the residue was purified by preparative HPLC to afford the title compound (1.31 g, 47%) as a white solid. LC-MS: mlz = 350.2 (MH+), tR = 2.14 min, method D.
The following compounds of the invention were prepared analogously:
5,7-Dimethyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine. LC-MS: m/z = 350.3 (MH+), fR = 0.76 min, method A.
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-1 -phenyl-1 H-benzoimidazole (This reaction was run using DIPEA as base). LC-MS: m/z = 396.8 (MH+), fR = 0.60 min, method C.
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2-a]pyrimidine (This reaction was run at 70 0C overnight using DIPEA as base). LC-MS: mlz = 322.1 (MH+), fR = 0.36 min, method C.
8-Methyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2-a]pyridine (This reaction was run at 60 0C for 1 h using DIPEA as base). LC-MS: mlz = 335.3 (MH+), tR = 0.55 min, method A. 2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2-a]pyridine (This reaction was run at 60 0C for 1 h using DIPEA as base). LC-MS: m/z = 321.0 (MH+), fR = 0.47 min, method A.
8-Methyl-2-(4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2-a]pyridine (This reaction was run at 60 0C for 1 h using DIPEA as base). LC-MS: m/z = 321.2 (MH+), fR = 0.48 min, method A.
2-[4-(3-Methoxy-phenyl)-1-methyl-1 H-imidazol-2-ylsulfanylmethyl]-5,7-dimethyl- imidazo[1 ,2-a]pyrimidine (This reaction was run at 60 0C for 2 h using DIPEA as base). LC-MS: m/z = 380.6 (MH+), tR = 0.65 min, method A.
5,7-Dimethyl-2-(4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2-a]pyrimidine (This reaction was run at 70 0C for 1 h using DIPEA as base). LC-MS: m/z = 336.3 (MH+), tR = 0.54 min, method A.
5,7-Dimethyl-2-(4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-[1 ,2,4]triazolo[1 ,5- a]pyrimidine, LC-MS (MH+): m/z = 337.4, fR (min, method A) =0.58
5,7-Dimethyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-[1 ,2,4]triazolo[1 ,5- a]pyrimidine, LC-MS (MH+): m/z = 351.4, fR (min, method A) =0.58
5-Ethyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine, LC-MS (MH+): m/z = 350.5, tR (min, method A) =0.76
Example 6
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-pyrazolo[1 ,5-a]pyridine
Figure imgf000059_0001
A solution of methanesulfonyl chloride (0.149 g, 0.13 mmol) in dichloromethane (2 ml_) was added to a solution of pyrazolo[1 ,5-a]pyridin-2-yl-methanol (0.148 g, 0.1 mmol) and triethylamine (0.303 g, 0.3 mmol) in dichloromethane (3 ml_) at -10 0C under N2. After the addition was complete, the mixture was stirred at -10 0C for 1 h. Ice water (10 ml_) was added, and the organic layer was separated, dried over sodium sulfate, filtered and concentrated under vacuum to give methanesulfonic acid pyrazolo[1 ,5-a]pyridin-2- ylmethyl ester as a yellow oil, which was used for the next step without further purification. A solution of this material (0.22 g, 0.1 mmol) in dry dichloromethane (2 ml_) was added to a solution of 1-methyl-4-phenyl-1 ,3-dihydro-imidazole-2-thione (0.190 g, 0.1 mmol) and DIPEA (0.303 g, 0.3 mmol) in dry DMF (3 ml_) at -10 0C under N2. The mixture was stirred at 0 0C for 2 hours, and was then concentrated under reduced pressure. The residue was purified by preparative HPLC to afford the title compound (50 mg, 15%). LC-MS: m/z = 321.1 (MH+), tR = 2.16 min, method F.
Example 7
2-(1-Benzyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-5,7-dimethyl- [1,2,4]triazolo[1,5-a]pyridine
Figure imgf000059_0002
To a solution of 5,7-dimethyl-2-(4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)- [1 ,2,4]triazolo[1 ,5-a]pyridine (15 mg, 0.045 mmol) in DMF (0.5 mL) was added benzyl bromide (5.4 μL, 0.045 mmol) and potassium carbonate (9.3 mg, 0.067 mmol) and the resulting mixture was stirred overnight at 75 0C. Volatiles were evaporated and the residue was purified by preparative LC-MS to yield the title compound. LC-MS: m/z = 426.3 (MH+), tR = 1.06 min, method A.
The following compounds of the invention were prepared analogously:
2-[1-(4-Chloro-benzyl)-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine. LC-MS: m/z = 460.7 (MH+), tR = 1.16 min, method A.
5,7-Dimethyl-2-(4-phenyl-1 -propyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2-a] pyrimidine. LC-MS: m/z = 378.6 (MH+), tR = 0.80 min, method A.
2-(1 -lsopropyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-5,7-dimethyl-imidazo[1 ,2-a] pyrimidine. LC-MS: m/z = 378.6 (MH+), tR = 0.78 min, method A.
2-(1-Cyclopropylmethyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-5,7-dimethyl- imidazo[1 ,2-a]pyrimidine. LC-MS: m/z = 390.4 (MH+), tR = 0.83 min, method A. 5,7-Dimethyl-2-[1 -(3-methyl-butyl)-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl]- imidazo[1 ,2-a]pyrimidine. LC-MS: m/z = 406.6 (MH+), tR = 0.99 min, method A.
2-[2-(5,7-Dimethyl-imidazo[1 ,2-a]pyrimidin-2-ylmethylsulfanyl)-4-phenyl-imidazol-1-yl]- acetamide. LC-MS: m/z = 393.5 (MH+), tR = 0.52 min, method A.
5,7-Dimethyl-2-[4-phenyl-1-(tetrahydro-pyran-4-ylmethyl)-1 H-imidazol-2-ylsulfanyl- methyl]-imidazo[1 ,2-a]pyrimidine. LC-MS: m/z = 434.6 (MH+), tR = 0.77 min, method A.
[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyridin-2-ylsulfanylmethyl)-4-phenyl-imidazol-1 -yl]- acetonitrile. LC-MS: m/z = 375.2 (MH+), tR = 0.70 min, method A.
2-(1 -lsopropyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-5,7-dimethyl[1 ,2,4]triazolo [1 ,5- a]pyridine. LC-MS: m/z = 378.5 (MH+), tR = 0.79 min, method A. 2-(1 -Cyclopropylmethyl-4-phenyl-i H-imidazol-2-ylmethylsulfanyl)-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine. LC-MS: m/z = 390.5 (MH+), tR = 0.85 min, method A.
2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyridin-2-ylsulfanylmethyl)-4-phenyl-imidazol-1 - yl]-acetamide. LC-MS: m/z = 393.5 (MH+), tR = 0.51 min, method A.
[2-(5,7-Dimethyl-imidazo[1 ,2-a]pyrimidin-2-ylmethylsulfanyl)-4-phenyl-imidazol-1-yl]- acetonitrile. LC-MS: m/z = 375.2 (MH+), tR = 0.93 min, method A.
2-(1 -Benzyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-5,7-dimethyl-imidazo[1 ,2- a]pyrimidine. LC-MS: m/z = 426.2 (MH+), tR = 1.08 min, method A. 2-[1-(4-Chloro-benzyl)-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl]-5,7-dimethyl- imidazo[1 ,2-a]pyrimidine. LC-MS: m/z = 460.5 (MH+), fR = 1.18 min, method A. 2-(1 -Ethyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-5,7-dimethyl-imidazo[1 ,2-a] pyrimidine. LC-MS: m/z = 364.5 (MH+), fR = 0.70 min, method A.
Example 8
5,7-Dimethyl-2-[1-(2-morpholin-4-yl-ethyl)-4-phenyl-1 H-imidazol-2- ylsulfanylmethyl]-imidazo[1,2-a]pyrimidine
Figure imgf000061_0001
A solution of methyl lithium in ether (1.60 M, 0.205 ml_, 0.328 mmol) was added dropwise to dimethyl sulfoxide (2.00 mL, 28.2 mmol) and the mixture left to stir for 40 minutes at room temperature. A solution of 5,7-Dimethyl-2-(4-phenyl-1 H-imidazol-2- ylsulfanylmethyl)-imidazo[1 ,2-a]pyrimidine (0.100 g, 0.298 mmol) and Λ/-(2- chloroethyl)morpholine (0.0666 g, 0.358 mmol) in dimethyl sulfoxide was added dropwise to the generated dimsyl anion. The resulting mixture was stirred at 80 °C for 45 minutes. After cooling to room temperature, water was carefully added and the mixture was extracted with ethyl acetate (20 mL). The combined organic extracts were dried over sodium sulfate and the solvent removed in vacuo. Column chromatography of the crude product using ethyl acetate:methanol (95:5 v/v) gave the product as a yellow oil. It was dissolved in a minimum amount of methanol and ethereal hydrogen chloride was added dropwise to precipitate the hydrochloride salt of title compound as a yellow solid which was collected by filtration and washed with ether (71 mg, 49 %). LC- MS: m/z = 449.3 (MH+), tR = 0.37 min, method C.
The Following compounds were prepared analogously:
4-(2-(2-((8-chloro-[1 ,2,4]triazolo[1 ,5-a]pyridin-2-yl)methylthio)-4-phenyl-1 H-imidazol-1 ■ yl)ethyl)morpholine, LC-MS (MH+): m/z = 456.0, tR (min, method A) =2.08 4-(2-(2-((5-chloro-[1 ,2,4]triazolo[1 ,5-a]pyridin-2-yl)methylthio)-4-phenyl-1 H-imidazol-1 - yl)ethyl)morpholine, LC-MS (MH+): m/z = 456.0, tR (min, method A) =2.16
2-(1 -lsobutyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-5,7-dimethyl-[1 ,2,4]triazolo[1 ,5- a]pyrimidine, LC-MS (MH+): mlz = 393.5, tR (min, method A) =0.88
5,7-Dimethyl-2-[1-(2-morpholin-4-yl-ethyl)-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl]- [1 ,2,4]triazolo[1 ,5-a]pyrimidine, LC-MS (MH+): m/z = 450.6, tR (min, method A) =0.55
1 -{2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidin-2-ylmethylsulfanyl)-4-phenyl- imidazol-1-yl]-ethyl}-3-methyl-imidazolidin-2-one, LC-MS (MH+): m/z = 463.6, tR (min, method A) =0.66
4-(2-(2-((8-methyl-[1 ,2,4]triazolo[1 ,5-a]pyridin-2-yl)methylthio)-4-phenyl-1 H-imidazol-1 - yl)ethyl)morpholine, LC-MS (MH+): m/z = 417.5, tR (min, method A) = 2.26
4-(2-(2-((5-methyl-[1 ,2,4]triazolo[1 ,5-a]pyridin-2-yl)methylthio)-4-phenyl-1 H-imidazol-1 - yl)ethyl)morpholine, LC-MS (MH+): m/z = 417.5, tR (min, method A) = 2.22
4-(2-(2-(2-(5,7-dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyridin-2-yl)ethyl)-4-phenyl-1 H-imidazol-1 - yl)ethyl)morpholine, LC-MS (MH+): m/z = 431.6, tR (min, method A) = 2.26
2-(2-(1 -ethyl-4-phenyl-1 H-imidazol-2-yl)ethyl)-5,7-dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyridine, LC-MS (MH+): m/z = 346.4, tR (min, method A) = 2.5
5,7-dimethyl-2-(2-(4-phenyl-1 -propyl-1 H-imidazol-2-yl)ethyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine, LC-MS (MH+): m/z = 360.5, tR (min, method A) = 2.53
2-[2-(1 -lsobutyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-5-methyl-[1 ,2,4]triazolo[1 ,5-a]pyridine, LC-MS (MH+): m/z = 360.5, tR (min, method A) = 0.88 2-[2-(1 -lsopropyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-5-methyl-[1 ,2,4]triazolo[1 ,5- a]pyridine, LC-MS (MH+): m/z = 346.4, tR (min, method A) = 0.79
1 -Methyl-3-(2-{2-[2-(5-methyl-[1 ,2,4]triazolo[1 ,5-a]pyridin-2-yl)-ethyl]-4-phenyl-imidazol- 1-yl}-ethyl)-imidazolidin-2-one, LC-MS (MH+): m/z = 430.5, tR (min, method A) = 0.99
5-Methyl-2-{2-[4-phenyl-1 -(3-piperidin-1 -yl-propyl)-1 H-imidazol-2-yl]-ethyl}- [1 ,2,4]triazolo[1 ,5-a]pyridine, LC-MS (MH+): m/z = 429.6, tR (min, method A) = 0.38
5,7-Dimethyl-2-{2-[4-phenyl-1 -(2-piperidin-1 -yl-ethyl)-1 H-imidazol-2-yl]-ethyl}-
[1 ,2,4]triazolo[1 ,5-a]pyrimidine, LC-MS (MH+): m/z = 430.6, tR (min, method A) =0.46
2-[2-(1 -lsobutyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-5,7-dimethyl-[1 ,2,4]triazolo[1 ,5- a]pyrimidine, LC-MS (MH+): mlz = 375.5, tR (min, method A) =0.8
2-[2-(1 -lsopropyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-5,7-dimethyl-[1 ,2,4]triazolo[1 ,5- a]pyrimidine, LC-MS (MH+): mlz = 361.5, tR (min, method A) =0.7
1 -(2-{2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidin-2-yl)-ethyl]-4-phenyl-imidazol-1 - yl}-ethyl)-3-methyl-imidazolidin-2-one, LC-MS (MH+): m/z = 445.5, tR (min, method A) =0.61
5,7-Dimethyl-2-{2-[1-(2-morpholin-4-yl-ethyl)-4-phenyl-1 H-imidazol-2-yl]-ethyl}- [1 ,2,4]triazolo[1 ,5-a]pyrimidine, LC-MS (MH+): m/z = 432.5, tR (min, method A) =0.44
5,7-Dimethyl-2-[2-(4-phenyl-1 -propyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- a]pyrimidine, LC-MS (MH+): m/z = 361.5, tR (min, method A) =0.71 Example 9 frans-5,7-Dimethyl-2-[(E)-2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]- imidazo[1 ,2-a]-pyrimidine
Figure imgf000064_0001
A microwave vial was charged with 2-chloromethyl-5,7-dimethyl-imidazo[1 ,2- a]pyrimidine hydrochloride (500 mg, 2.15 mmol), and 1 ,2-dichloroethane (16 mL) and argon was bubbled through the mixture. To this mixture was added 1 ,8- diazabicyclo[5.4.0]undec-7-ene (0.350 mL, 2.34 mmol) and triphenylphosphine (848 mg, 3.23 mmol). The vial was sealed with a crimp-on cap and the mixture was heated at 140 0C for 1 hour using a microwave synthesizer. Evaporation of volatiles afforded crude (5,7-dimethyl-imidazo[1 ,2-a]pyrimidin-2-ylmethyl)-triphenyl-phosphonium chloride as a dark grey solid (1.976 g) which was used without purification.
A solution of 1-methyl-4-phenyl-1 H-imidazole-2-carbaldehyde (109 mg, 0.585 mmol) in dry THF was added to (5,7-dimethyl-imidazo[1 ,2-a]pyrimidin-2-ylmethyl)-triphenyl- phosphonium chloride (536 mg, 0.585 mmol) under argon and 1 ,8-diazabicyclo [5.4.0]undec-7-ene (87.5 μl_, 0.585 mmol) was added. The reaction mixture was stirred at room temperature for 3 days after which it was evaporated onto Celite®. Silica gel chromatography (gradient elution; A:B 0:100 -> 100:0, where A is 10 % methanol in ethyl acetate and B is heptane) afforded a mixture of the title compound and the phosphonium starting material. This mixture was dissolved in dry THF and was re- submitted to the reaction conditions using 120 mg of aldehyde and 90 μl of diazabicyclo[5.4.0]undec-7-ene with an overnight reaction time at room temperature. Chromatography using the conditions above afforded the title compound (35 mg, 18%) as a brown solid. LC-MS: mlz = 329.8 (MH+), fR = 0.36 min, method C.
The Following compounds were prepared analogously:
8-Methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]-[1 ,2,4]triazolo[1 ,5-a]pyridine 5-Methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]-[1 ,2,4]triazolo[1 ,5-a]pyridine
5,7-dimethyl-2-(2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)vinyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine
6,8-dimethyl-2-(2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)vinyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine
5,7-dimethyl-2-(2-(4-phenyl-1 H-imidazol-2-yl)vinyl)-[1 ,2,4]triazolo[1 ,5-a]pyridine
5,7-Dimethyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]-[1 ,2,4]triazolo[1 ,5- a]pyrimidine
5,7-Dimethyl-2-[2-(4-phenyl-1 H-imidazol-2-yl)-vinyl]-[1 ,2,4]triazolo[1 ,5-a]pyrimidine
5,8-Dimethyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]-[1 ,2,4]triazolo[1 ,5- a]pyridine
5-Methyl-2-[2-(4-phenyl-1 H-imidazol-2-yl)-vinyl]-[1 ,2,4]triazolo[1 ,5-a]pyridine
5,6,7-Trimethyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]-[1 ,2,4]triazolo[1 ,5- a]pyrimidine
5-Methyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]-7-phenyl-[1 ,2,4]triazolo[1 ,5- a]pyrimidine
5-Methyl-2-{2-[4-phenyl-1 -(2-piperidin-1 -yl-ethyl)-1 H-imidazol-2-yl]-vinyl}- [1 ,2,4]triazolo[1 ,5-a]pyridine Example 10
5,7-Dimethyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-imidazo[1 ,2- a]pyrimidine
Figure imgf000066_0001
To a solution of trans-5,7-dimethyl-2-[(E)-2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]- imidazo[1 ,2-a]pyrimidine (13.0 mg, 0.0395 mmol) in ethanol (4 ml_) was added 10% palladium on carbon (9 mg). optionally, a catalytic amount of acid e.g. trifluoracetic acid, can be added. A current of hydrogen gas was bubbled through, and the reaction was kept under an atmosphere of hydrogen overnight with stirring. Filtration and evaporation of volatiles afforded the title compound (9.8 mg, 75%). LC-MS: m/z = 332.3 (MH+), tR = 0.37 min, method A.
The Following compounds were prepared analogously:
8-Methyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5-a]pyridine, LC-MS (MH+): m/z = 318.4, tR (min, method A) = 2.2
5-Methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5-a]pyridine, LC-MS (MH+): m/z = 318.4, tR (min, method A) = 2.44
5,7-dimethyl-2-(2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)ethyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine, LC-MS (MH+): m/z = 332.4, tR (min, method A) = 2.57
6,8-dimethyl-2-(2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)ethyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine, LC-MS (MH+): m/z = 332.4, tR (min, method A) = 2.65
5,7-dimethyl-2-(2-(4-phenyl-1 H-imidazol-2-yl)ethyl)-[1 ,2,4]triazolo[1 ,5-a]pyridine LC- MS (MH+): m/z = 318.4, tR (min, method A) = 2.61 5,7-Dimethyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- a]pyrimidine, LC-MS (MH+): m/z = 333.4, tR (min, method A) = 0.57
5,7-Dimethyl-2-[2-(4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5-a]pyrimidine, LC- MS (MH+): m/z = 319.4, tR (min, method A) = 0.57
5,8-Dimethyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- a]pyridine, LC-MS (MH+): m/z = 332.4, tR (min, method A) = 0.71
5-Methyl-2-[2-(4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5-a]pyridine, LC-MS (MH+): m/z = 304.4, tR (min, method A) = 0.6
5,6,7-Trimethyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- a]pyrimidine, LC-MS (MH+): m/z = 347.4, tR (min, method A) = 0.63
5-Methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-7-phenyl-[1 ,2,4]triazolo[1 ,5- a]pyrimidine, LC-MS (MH+): m/z = 395.5, tR (min, method A) = 0.8
5-Methyl-2-{2-[4-phenyl-1 -(2-piperidin-1 -yl-ethyl)-1 H-imidazol-2-yl]-ethyl}- [1 ,2,4]triazolo[1 ,5-a]pyridine, LC-MS (MH+): m/z = 415.6, tR (min, method A) = 0.5
Example 11 trans- 5,8-Dimethyl-2-[(E)-2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]-
[1 ,2,4]triazolo[1 ,5-a]pyrazine
Figure imgf000068_0001
A solution of 2-chloromethyl-5,8-dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrazine (1.351 g, 6.87 mmol) and triphenylphosphine (1.80 g, 6.87 mmol) in acetonitrile 150 ml_ was heated at reflux for 12 h. The solvents were removed in vacuo and the residue slurried in ether, filtered and dried to yield (5,8-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrazin-2-ylmethyl)- triphenyl-phosphonium; chloride as an off white solid (2.412 g, 74.9%). LC-MS: m/z = 423.2 ([M-CI]+), tR = 0.86 min, method A.
A solution of 1-methyl-4-phenyl-1 H-imidazole-2-carbaldehyde (220 mg, 1.18 mmol) in dry THF was added to (5,8-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrazin-2-ylmethyl)-triphenyl- phosphonium; chloride (500 mg, 1.18 mmol) under argon and 1 ,8-diazabicyclo [5.4.0]undec-7-ene (176 μl_, 1.18 mmol) was added. The reaction mixture was stirred at room temperature for 2 h after which it was evaporated onto silica gel (2 g). Silica gel chromatography (gradient elution; A:B 50:50 -> 100:0, where A is ethyl acetate and B is heptane) afforded the title compound (334 mg, 79%) as an off white solid. LC-MS: m/z = 331.4 (MH+), tR = 0.65 min, method A.
The Following compounds were prepared analogously and were used for the preparation of final compounds without prior purification or characterization:
trans-λ -{2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidin-2-yl)-vinyl]-4-phenyl-imidazol- 1-yl}-propan-2-ol trans-{β)-λ -{2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidin-2-yl)-vinyl]-4-phenyl- imidazol-1 -yl}-propan-2-ol frans-8-methoxy-5-methyl-2-(2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)vinyl)- [1 ,2,4]triazolo[1 ,5-a]pyridine trans- (R)-1 -{2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidin-2-yl)-vinyl]-4-phenyl- imidazol-1 -yl}-propan-2-ol frans-8-fluoro-2-(2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)vinyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine trans-λ -{2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidin-2-yl)-vinyl]-4-phenyl-imidazol-
1 -yl}-2-methyl-propan-2-ol frans-8-Ethyl-5-methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]-
[1 ,2,4]triazolo[1 ,5-c]pyrimidine frans-5-Methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]-7-propyl- [1 ,2,4]triazolo[1 ,5-a]pyrimidine frans-7-Methoxy-5-methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]-
[1 ,2,4]triazolo[1 ,5-c]pyrimidine frans-7-lsopropyl-5-methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]-
[1 ,2,4]triazolo[1 ,5-a]pyrimidine frans-2-{2-[4-(2,4-Difluoro-phenyl)-1 -methyl-1 H-imidazol-2-yl]-vinyl}-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyrimidine frans-7-Methoxy-5,8-dimethyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]-
[1 ,2,4]triazolo[1 ,5-c]pyrimidine frans-5,8-Dimethyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]-[1 ,2,4]triazolo[1 ,5- c]pyrimidine frans-2-{2-[4-(2-Methoxy-phenyl)-1 -methyl-1 H-imidazol-2-yl]-vinyl}-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyrimidine frans-{5-Methyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]-[1 ,2,4]triazolo[1 ,5- a]pyrimidin-7-yl}-methanol frans-8-Ethyl-5-methyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]-
[1 ,2,4]triazolo[1 ,5-a]pyridine frans-5,8-Dimethoxy-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]-
[1 ,2,4]triazolo[1 ,5-a]pyridine Example 12
5,8-Dimethyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1,2,4]triazolo[1,5- a]pyrazine
Figure imgf000070_0001
A solution of trans-5,8-dimethyl-2-[(E)-2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]- imidazo[1 ,2-a]pyrazine (330 mg, 1.0 mmol) in methanol (50 ml_) was passed through a H-Cube® Continuous-flow Hydrogenation Reactor (ThalesNano) at a flow rate of 1 mL/min through a small cartridge of 10% Pd/C (THS01111 ) with an internal temperature of 25°C and 1 bar of hydrogen pressure. Evaporation of the volatiles afforded the title compound (178 mg, 51 %). LC-MS: mlz = 333.2 (MH+), tR = 0.57 min, method A.
The Following compounds were prepared analogously:
5,8-Dimethyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-imidazo[1 ,2-c]pyrimidine, LC-MS: m/z = 333.2 (MH+), tR = 0.67 min, method E.
1 -{2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidin-2-yl)-ethyl]-4-phenyl-imidazol-1 -yl}- propan-2-ol, LC-MS: m/z = 377.4 (MH+), fR = 0.58 min, method A.
(S)-1 -{2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidin-2-yl)-ethyl]-4-phenyl-imidazol-1 - yl}-propan-2-ol, LC-MS: m/z = 377.4 (MH+), fR = 0.58 min, method A.
(R)- 1 -{2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidin-2-yl)-ethyl]-4-phenyl-imidazol-1 - ylJ-propan-2-ol, LC-MS: mlz = 377.4 (MH+), tR = 0.59 min, method A.
1 -{2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidin-2-yl)-ethyl]-4-phenyl-imidazol-1 -yl}- 2-methyl-propan-2-ol, LC-MS: m/z = 391.8 (MH+), fR = 0.64 min, method A. 8-methoxy-5-methyl-2-(2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)ethyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine, LC-MS: m/z = 348.4 ([M-CI]+), tR = 0.77 min, method E.
8-fluoro-2-(2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)ethyl)-[1 ,2,4]triazolo[1 ,5-a]pyridine, LC-MS: m/z = 322.4 (MH+), tR = 0.60 min, method A.
8-Ethyl-5-methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- c]pyrimidine, LC-MS: m/z = 347.4 (MH+), tR = 0.67 min, method A.
5-Methyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-7-propyl-[1 ,2,4]triazolo[1 ,5- a]pyrimidine, LC-MS: m/z = 361.5 (MH+), tR = 0.74 min, method A.
7-Methoxy-5-methyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- c]pyrimidine, LC-MS: mlz = 349.4 (MH+), tR = 0.63 min, method A.
7-lsopropyl-5-methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]- [1 ,2,4]triazolo[1 ,5-a]pyrimidine, LC-MS: mlz = 361.5 (MH+), tR =0.74 min, method A.
2-{2-[4-(2,4-Difluoro-phenyl)-1-methyl-1 H-imidazol-2-yl]-ethyl}-5,7-dimethyl- [1 ,2,4]triazolo[1 ,5-a]pyrimidine, LC-MS: m/z = 369.4 (MH+), tR = 0.64 min, method A.
7-Methoxy-5,8-dimethyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]- [1 ,2,4]triazolo[1 ,5-c]pyrimidine, LC-MS: mlz = 363.4 (MH+), tR = 0.78 min, method A.
5,8-Dimethyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- φyrimidine, LC-MS: m/z = 333.4 (MH+), tR = 0.58 min, method A.
2-{2-[4-(2-Methoxy-phenyl)-1-methyl-1 H-imidazol-2-yl]-ethyl}-5,7-dimethyl- [1 ,2,4]triazolo[1 ,5-a]pyrimidine, LC-MS: m/z = 363.4 (MH+), tR = 0.62 min, method A.
{5-Methyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- a]pyrimidin-7-yl}-methanol, LC-MS: mlz = 349.4 (MH+), tR = 0.47 min, method A. 8-Ethyl-5-methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- a]pyridine, LC-MS: m/z = 346.4 (MH+), fR =0.93 min, method E.
5,8-Dimethoxy-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- a]pyridine, LC-MS: mlz = 364.4 (MH+), fR = 0.70 min, method E.
Pharmacological Testing
PDE1 OA enzyme
Active PDE10A enzyme is prepared in a number of ways for use in PDE assays (Loughney, K. et al. Gene 1999, 234, 109-117; Fujishige, K. et al. Eur J Biochem. 1999, 266, 1118-1127 and Soderling, S. et al. Proc. Natl. Acad. Sci. 1999, 96, 7071-7076). PDE10A can be expressed as full-length proteins or as truncated proteins, as long as they express the catalytic domain. PDE10A can be prepared in different cell types, for example insect cells or E. coli. An example of a method to obtain catalytically active PDE10A is as follows: The catalytic domain of human PDE10A (amino acids 440-779 from the sequence with accession number NP 006652) is amplified from total human brain total RNA by standard RT-PCR and is cloned into the BamH1 and Xho1 sites of the pET28a vector (Novagen). Expression in coli is performed according to standard protocols. Briefly, the expression plasmids are transformed into the BL21 (DE3) E. coli strain, and 50 mL cultures inoculated with the cells allowed to grow to an OD600 of 0.4- 0.6 before protein expression is induced with 0.5mM IPTG. Following induction, the cells are incubated overnight at room temperature, after which the cells are collected by centrifugation. Cells expressing PDE10A are resuspended in 12 mL (50 mM TRIS-HCI- pH8.0, 1 mM MgCb and protease inhibitors). The cells are lysed by sonication, and after all cells are lysed, TritonXIOO is added according to Novagen protocols. PDE10A is partially purified on Q sepharose and the most active fractions were pooled.
PDE10A inhibition assay
A PDE10A assay may for example, be performed as follows: The assay is performed in 60 uL samples containing a fixed amount of the relevant PDE enzyme (sufficient to convert 20-25% of the cyclic nucleotide substrate), a buffer (50 mM HEPES7.6; 1OmM MgCI2; 0.02% Tween20), 0.1mg/ml BSA, 225 pCi of 3H-labelled cyclic nucleotide substrate, tritium labeled cAMP to a final concentration of 5 nM and varying amounts of inhibitors. Reactions are initiated by addition of the cyclic nucleotide substrate, and reactions are allowed to proceed for one hr at room temperature before being terminated through mixing with 15 uL 8 mg/mL yttrium silicate SPA beads (Amersham). The beads are allowed to settle for one hr in the dark before the plates are counted in a Wallac 1450 Microbeta counter. The measured signal can be converted to activity relative to an uninhibited control (100 %) and IC5O values can be calculated using the Xlfit extension to EXCEL.
In the context of the present invention the assay was performed in 60 uL assay buffer (50 mM HEPES pH 7.6; 1OmM MgCI2; 0.02% Tween20) containing enough PDEIOA to convert 20-25% of 10 nM 3H-CAMP and varying amounts of inhibitors. Following a 1 hour incubation the reactions were terminated by addition of 15 uL 8 mg/mL yttrium silicate SPA beads (Amersham). The beads were allowed to settle for one hr in the dark before the plates were counted in a Wallac 1450 Microbeta counter. IC5O values were calculated by non linear regression using XLfit (IDBS).
Results of the experiments showed that the tested compounds of the invention inhibit the PDE10A enzyme with IC50 values below 700 nM.
Results of the experiments showed that the majority of the compounds of the invention had IC5O values of <1500nM, many compounds <100nM, some compounds <50nM and some had IC5O values <10nM.
Phencyclidine (PCP) induced hyperactivity
Male mice (NMRI, Charles River) weighing 20-25g are used. Eight mice are used in each group receiving the test compound (5 mg/kg) plus PCP (2.3 mg/kg) including the parallel control groups receiving the vehicle of the test compound plus PCP or vehicle injections only. The injection volumen is 10 ml/kg. The experiment is made in normal light conditions in an undisturbed room. The test substance is injected per oss 60 min before injection of PCP, which is administered subcutaneous. Immediately after injection of PCP the mice are placed individually in special designed test cage (20 cm x 32 cm). The activity is measured by 5X8 infrared light sources and photocells spaced by 4 cm. The light beams cross the cage 1.8 cm above the bottom of the cage. Recording of a motility count requires interruption of adjacent light beams, thus avoiding counts induced by stationary movements of the mice.
Motility is recorded in 5 min intervals for a period of 1 hour. The drug effect is calculated on the total counts during the 1 hour behavioral test period in the following manner: The mean motility induced by vehicle treatment in the absence of PCP is used as baseline. The 100 per cent effect of PCP is accordingly calculated to be total motility counts minus baseline. The response in groups receiving test compound is thus determined by the total motility counts minus baseline, expressed in per cent of the similar result recorded in the parallel PCP control group. The per cent responses are converted to per cent inhibition.
Results of the experiments showed that the tested compounds of the invention are in vivo active compounds that inhibit the PCP induced hyperactivity to the % shown in table 2 above.

Claims

What is claimed:
1. A compound having the structure I
Figure imgf000075_0001
wherein HET is a heteroaromatic group of formula Il containing from 2 to 4 nitrogen atoms:
Figure imgf000075_0002
II wherein Y can be N or CH, Z can be N or C, and wherein HET may optionally be substituted with up to three substituents R7-Rg individually selected from hydrogen, Ci-Cβ alkyl; halogen; cyano, halo(Ci-C6)alkyl; aryl, alkoxy and Ci-Cε hydroxyalkyl, and wherein * denotes the attachment point,
-L- is a linker selected from -CH2-CH2-, -S-CH2- , -CH2-S- or -CH=CH- ,
Ri is selected from H, d-C6 alkyl; d-C6 alkyl(C3-C8)cycloalkyl; d-C6 hydroxyalkyl, CH2CN, CH2C(O)NH2, d-C6 arylalkyl, and d-C6 alkyl- heterocycloalkyl,
R2-R6 are selected individually from H, CrCβ alkoxy and halogen,
and tautomers and pharmaceutically acceptable acid addition salts thereof, and polymorphic forms thereof, provided that the compound is not 2-(5-phenyl-1 H- imidazol-2-ylmethylsulfanyl)-1 H-benzoimidazole or 2-(5-phenyl-1 H-imidazol-2-yl- sulfanyl-methyl)-1 H-benzoimidazole.
2. The compound of Claim 1 in which HET is selected from the group consisting of [1 ,2,4]Triazolo[1 ,5-a]pyrazine, imidazo[1 ,2-a]pyrimidine, imidazo[4,5-b]pyrimidine,
[1 ,2,4]Triazolo[1 ,5-a]pyrimidine, [1 ,2,4]Triazolo[1 ,5-c]pyrimidine, 5,7-Dimethyl- [1 ,2,4]triazolo[1 ,5-a]pyrimidine and 5,7-dimethyl-imidazo[1 ,2-a]pyrimidine.
3. The compound of Claim 1 in which HET is selected from the group consisting of [1 ,2,4]triazolo[1 ,5-a]pyridine, imidazo[1 ,2-a]pyridine, pyrazolo[1 ,5-a]pyridine, 5,7- dimethyl-[1 ,2,4]triazolo[1 ,5-a] pyridine, 5,7-dimethyl-imidazo[1 ,2-a]pyridine, 5- chloro-imidazo[1 ,2-a]pyridine, 5-methyl-imidazo [1 ,2-a]pyridine, 5-trifluoromethyl- imidazo [1 ,2-a]pyridine, 6-Bromo-5,7-dimethyl-[1 ,2,4] triazolo[1 ,5-a] pyridine, 6- bromo-7-methyl-[1 ,2,4]triazolo[1 ,5-a] pyridine, 6-chloro-8-methyl- [1 ,2,4]triazolo[1 ,5-a] pyridine, 6-chloro-imidazo[1 ,2-a]pyridine, 7-methyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine and 8-methyl-imidazo[1 ,2-a]pyridine.
4. The compound of Claim 1 in which HET is selected from the group consisting of 1-methyl-1 H-benzoimidazole and 1-phenyl-1 H-benzoimidazole.
5. The compound of Claim 1 in which HET is [1 ,2,4]triazolo[1 ,5-a]pyridine-6- carbonitrile or imidazo[1 ,2-a] pyridine-7-carbonitrile.
6. The compound of Claim 1 in which HET is 2-(6-chloro-benzoimidazol-1-yl)- ethanol.
7. The compound of any one of Claim 1-6 wherein -L- is -CH2-CH2-
8. The compound of any one of Claim 1-6 wherein -L- is -CH2-S-
9. The compound of any one of Claim 1-6 wherein -L- is -S-CH2-
10. The compound of any one of Claim 1 -6 wherein -L- is -CH=CH-
11. The compound of any one of Claim 1-10 wherein R1 is hydrogen
12. The compound of any one of Claim 1-10 wherein R1 is not hydrogen
13. The compound of any one of Claim 1-12 wherein R2, R3, R4, R5 and Rε are all hydrogen.
14. The compound of any one of Claim 1-12 wherein at least one of R2, R3, R4, R5 and Re is Ci-Ce alkoxy, such as methoxy.
15. The compound of any one of Claim 1-12 wherein at least one of R2, R3, R4, R5 and Rε is halogen, such as chlorine or fluorine.
16. The compound of any one of Claim 1-15 wherein R7, R8 and Rg are all hydrogen.
17. The compound of any one of Claim 1-15 wherein at least one of R7, R8 and Rg is Ci-Cε alkyl, such as methyl.
18. The compound of any one of Claim 1-15 wherein at least one of R7, R8 and Rg is Ci-Cε alkoxy.
19. The compound of any one of Claim 1-15 wherein at least one of R7, R8 and Rg is halogen, such as chlorine or bromine.
20. The compound of claim 1 , wherein the compound is selected from the group consisting of:
5,7-Dimethyl-2-[1-(3-methyl-butyl)-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl]- imidazo[1 ,2-a]pyrimidine; 5,7-Dimethyl-2-(4-phenyl-1 -propyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2- a]pyrimidine;
2-(1-Cyclopropylmethyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-5,7-dimethyl- imidazo[1 ,2-a]pyrimidine; 5,7-dimethyl-2-((1 -methyl-4-phenyl-1 H-imidazol-2-ylthio)methyl)imidazo[1 ,2- a]pyrimidine;
5,7-Dimethyl-2-[1-(2-morpholin-4-yl-ethyl)-4-phenyl-1 H-imidazol-2- ylsulfanylmethyl]-imidazo[1 ,2-a]pyrimidine: 5,7-Dimethyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-
[1 ,2,4]triazolo[1 ,5-a]pyridine;
2-(1-Cyclopropylmethyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine;
2-(1-Benzyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-5,7-dimethyl- [1 ,2,4]triazolo[1 ,5-a]pyridine;
[2-(5,7-Dimethyl-imidazo[1 ,2-a]pyrimidin-2-ylmethylsulfanyl)-4-phenyl-imidazol-1- yl]-acetonitrile;
5,7-Dimethyl-2-[4-phenyl-1-(tetrahydro-pyran-4-ylmethyl)-1 H-imidazol-2- ylsulfanylmethyl]-imidazo[1 ,2-a]pyrimidine; 5,7-Dimethyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-
[1 ,2,4]triazolo[1 ,5-a]pyridine;
5,7-Dimethyl-2-(4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine;
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-5-trifluoromethyl-imidazo[1 ,2- a]pyridine;
2-(1 -Ethyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-5,7-dimethyl-[1 ,2,4]triazolo[1 ,5- a]pyridine;
5,7-Dimethyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-imidazo[1 ,2- a]pyrimidine; [2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyridin-2-ylsulfanylmethyl)-4-phenyl-imidazol-
1-yl]-acetonitrile;
2-(1-lsopropyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine;
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1 ,2,4]triazolo[1 ,5-a]pyridine; 2-(1 -Benzyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-5,7-dimethyl-imidazo[1 ,2- a]pyrimidine;
2-(4-Phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1 ,2,4]triazolo[1 ,5-a]pyridine 6-Chloro-8-methyl-2-(1-methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-
[1 ,2,4]triazolo[1 ,5-a]pyridine; trans-5,7-Dimethyl-2-[(E)-2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-vinyl]-imidazo[1 ,2- a]-pyrimidine; 2-(1 -lsopropyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-5,7-dimethyl-imidazo[1 ,2- a]pyrimidine;
2-[4-(3-Fluoro-phenyl)-1-methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine;
2-(1 -Ethyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-5,7-dimethyl-imidazo[1 ,2- a]pyrimidine;
2-(5,7-Dimethyl-imidazo[1 ,2-a]pyrimidin-2-ylmethylsulfanyl)-4-phenyl-imidazol-1- ylamine;
5,7-Dimethyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2- a]pyridine; 2-[4-(3-Methoxy-phenyl)-1 -methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine;
7-Methyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine;
2-[4-(3-Chloro-phenyl)-1 -methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl- [1 ,2,4]triazolo[1 ,5-a]pyridine;
2-[4-(4-Fluoro-phenyl)-1 -methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine
2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyridin-2-ylsulfanylmethyl)-4-phenyl- imidazol-1 -yl]-acetamide; 2-[4-(3-Methoxy-phenyl)-1 -methyl-1 H-imidazol-2-ylsulfanylmethyl]-5,7-dimethyl- imidazo[1 ,2-a]pyrimidine;
5-Chloro-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2- a]pyridine;
8-Methyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2- a]pyridine;
2-[4-(2-Fluoro-phenyl)-1 -methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine; 2-[4-(2-Chloro-phenyl)-1-methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine;
2-[2-(5,7-Dimethyl-imidazo[1 ,2-a]pyrimidin-2-ylmethylsulfanyl)-4-phenyl-imidazol-1- yl]-acetamide; 2-(1 -Ethyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1 ,2,4]triazolo[1 ,5-a]pyridine;
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-1 -phenyl-1 H-benzoimidazole;
2-[4-(2-Methoxy-phenyl)-1-methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine;
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2-a]pyridine-7- carbonitrile
2-(1 -lsopropyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine;
2-[1-(4-Chloro-benzyl)-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine; 6-Bromo-5,7-dimethyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-
[1 ,2,4]triazolo[1 ,5-a]pyridine;
2-[4-(3-Fluoro-phenyl)-1-methyl-1 H-imidazol-2-ylmethylsulfanyl]-[1 ,2,4]triazolo[1 ,5- a]pyridine;
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-pyrazolo[1 ,5-a]pyridine; 5-Methyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2- a]pyridine;
2-[4-(4-Fluoro-phenyl)-1-methyl-1 H-imidazol-2-ylmethylsulfanyl]-[1 ,2,4]triazolo[1 ,5- a]pyridine;
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-1 -phenyl-1 H-benzoimidazole; 2-[4-(3-Chloro-phenyl)-1 -methyl-1 H-imidazol-2-ylmethylsulfanyl]-[1 ,2,4]triazolo[1 ,5- a]pyridine;
2-(6-Chloro-imidazo[1 ,2-a]pyridin-2-ylmethylsulfanyl)-4-phenyl-imidazol-1-ylamine;
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-1 H-imidazo[4,5-b]pyridine;
6-Chloro-8-methyl-2-(1-methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)- imidazo[1 ,2-a]pyridine;
2-[4-(4-Chloro-phenyl)-1 -methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine; 6-Bromo-7-methyl-2-(1-methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-
[1 ,2,4]triazolo[1 ,5-a]pyridine;
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1 ,2,4]triazolo[1 ,5- a]pyrimidine; 2-[2-(1 -Amino-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-6-chloro-benzoimidazol-1 - yl]-ethanol;
2-(lmidazo[1 ,2-a]pyridin-2-ylmethylsulfanyl)-4-phenyl-imidazol-1-ylamine;
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2-a]pyridine;
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-[1 ,2,4]triazolo[1 ,5-a]pyridine- 6-carbonitrile;
2-[4-(4-Methoxy-phenyl)-1-methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyridine;
1 -Methyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-1 H-benzoimidazole;
2-(1 -Methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2-a]pyrimidine; 8-Methyl-2-(4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-imidazo[1 ,2-a]pyridine;
2-[1-(4-Chloro-benzyl)-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl]-5,7-dimethyl- imidazo[1 ,2-a]pyrimidine;
4-(2-(2-((8-chloro-[1 ,2,4]triazolo[1 ,5-a]pyridin-2-yl)methylthio)-4-phenyl-1 H- imidazol-1 -yl)ethyl)morpholine; 8-Methyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- a]pyridine;
8-Methyl-2-{2-[1-(2-morpholin-4-yl-ethyl)-4-phenyl-1 H-imidazol-2-yl]-ethyl}-
[1 ,2,4]triazolo[1 ,5-a]pyridine;
5-Methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- a]pyridine;
5-Methyl-2-{2-[1-(2-morpholin-4-yl-ethyl)-4-phenyl-1 H-imidazol-2-yl]-ethyl}-
[1 ,2,4]triazolo[1 ,5-a]pyridine;
4-(2-(2-((5-chloro-[1 ,2,4]triazolo[1 ,5-a]pyridin-2-yl)methylthio)-4-phenyl-1 H- imidazol-1 -yl)ethyl)morpholine; 5,7-dimethyl-2-(2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)ethyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine;
4-(2-(2-(2-(5,7-dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyridin-2-yl)ethyl)-4-phenyl-1 H- imidazol-1 -yl)ethyl)morpholine; 6,8-dimethyl-2-(2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)ethyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine;
5,7-dimethyl-2-(2-(4-phenyl-1 H-imidazol-2-yl)ethyl)-[1 ,2,4]triazolo[1 ,5-a]pyridine;
5,7-Dimethyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- a]pyrimidine;
2-(2-(1 -ethyl-4-phenyl-1 H-imidazol-2-yl)ethyl)-5,7-dimethyl-[1 ,2,4]triazolo[1 ,5- a]pyridine;
5,7-dimethyl-2-(2-(4-phenyl-1 -propyl-1 H-imidazol-2-yl)ethyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine; 5,7-Dimethyl-2-[2-(4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5-a]pyrimidine;
5,7-Dimethyl-2-(4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-[1 ,2,4]triazolo[1 ,5- a]pyrimidine;
5,8-Dimethyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- a]pyridine; 5,7-Dimethyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-
[1 ,2,4]triazolo[1 ,5-a]pyrimidine;
5-Methyl-2-[2-(4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5-a]pyridine;
2-(1-lsobutyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyrimidine; 5,7-Dimethyl-2-[1 -(2-morpholin-4-yl-ethyl)-4-phenyl-1 H-imidazol-2- ylsulfanylmethyl]-[1 ,2,4]triazolo[1 ,5-a]pyrimidine;
5-Methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-7-morpholin-4-yl-
[1 ,2,4]triazolo[1 ,5-a]pyrimidine;
2-[2-(1 -lsobutyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-5-methyl-[1 ,2,4]triazolo[1 ,5- a]pyridine;
2-[2-(1 -lsopropyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-5-methyl-[1 ,2,4]triazolo[1 ,5- a]pyridine;
1 -Methyl-3-(2-{2-[2-(5-methyl-[1 ,2,4]triazolo[1 ,5-a]pyridin-2-yl)-ethyl]-4-phenyl- imidazol-1-yl}-ethyl)-imidazolidin-2-one; 5-Methyl-2-{2-[4-phenyl-1 -(3-piperidin-1 -yl-propyl)-1 H-imidazol-2-yl]-ethyl}-
[1 ,2,4]triazolo[1 ,5-a]pyridine;
Diisopropyl-(2-{2-[2-(5-methyl-[1 ,2,4]triazolo[1 ,5-a]pyridin-2-yl)-ethyl]-4-phenyl- imidazol-1 -yl}-ethyl)-amine; 8-Methoxy-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine;
1 -{2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidin-2-ylmethylsulfanyl)-4-phenyl- imidazol-1-yl]-ethyl}-3-methyl-imidazolidin-2-one; 5,6,7-Trimethyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- a]pyrimidine;
5-Methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-7-phenyl-
[1 ,2,4]triazolo[1 ,5-a]pyrimidine;
5-Methyl-2-{2-[4-phenyl-1 -(2-piperidin-1 -yl-ethyl)-1 H-imidazol-2-yl]-ethyl}- [1 ,2,4]triazolo[1 ,5-a]pyridine;
2-[4-(3-Methoxy-phenyl)-1-methyl-1 H-imidazol-2-ylmethylsulfanyl]-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyrimidine;
5-Ethyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylsulfanylmethyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine; 5,7-Dimethyl-2-(1 -methyl-4-phenyl-1 H-imidazol-2-ylmethylsulfanyl)-
[1 ,2,4]triazolo[1 ,5-a]pyrimidine;
5,7-Dimethyl-2-{2-[4-phenyl-1 -(2-piperidin-1 -yl-ethyl)-1 H-imidazol-2-yl]-ethyl}-
[1 ,2,4]triazolo[1 ,5-a]pyrimidine;
2-[2-(1 -lsobutyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-5,7-dimethyl-[1 ,2,4]triazolo[1 ,5- a]pyrimidine;
2-[2-(1 -lsopropyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-5,7-dimethyl-[1 ,2,4]triazolo[1 ,5- a]pyrimidine;
1 -(2-{2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidin-2-yl)-ethyl]-4-phenyl- imidazol-1-yl}-ethyl)-3-methyl-imidazolidin-2-one; (2-{2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidin-2-yl)-ethyl]-4-phenyl-imidazol-
1 -yl}-ethyl)-diisopropyl-amine;
5,7-Dimethyl-2-{2-[1-(2-morpholin-4-yl-ethyl)-4-phenyl-1 H-imidazol-2-yl]-ethyl}-
[1 ,2,4]triazolo[1 ,5-a]pyrimidine;
5,7-Dimethyl-2-[2-(4-phenyl-1 -propyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- a]pyrimidine;
1 -{2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidin-2-yl)-ethyl]-4-phenyl-imidazol-1 - yl}-propan-2-ol; (S)-1 -{2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidin-2-yl)-ethyl]-4-phenyl- imidazol-1 -yl}-propan-2-ol;
8-methoxy-5-methyl-2-(2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)ethyl)-
[1 ,2,4]triazolo[1 ,5-a]pyridine; (R)-1 -{2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidin-2-yl)-ethyl]-4-phenyl- imidazol-1 -yl}-propan-2-ol;
8-fluoro-2-(2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)ethyl)-[1 ,2,4]triazolo[1 ,5- a]pyridine;
1 -{2-[2-(5,7-Dimethyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidin-2-yl)-ethyl]-4-phenyl-imidazol-1 ■ yl}-2-methyl-propan-2-ol;
8-Ethyl-5-methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-
[1 ,2,4]triazolo[1 ,5-c]pyrimidine;
5-Methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-7-propyl-
[1 ,2,4]triazolo[1 ,5-a]pyrimidine; 5,8-Dimethyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- a]pyrazine;
7-Methoxy-5-methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-
[1 ,2,4]triazolo[1 ,5-c]pyrimidine;
7-lsopropyl-5-methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]- [1 ,2,4]triazolo[1 ,5-a]pyrimidine
2-{2-[4-(2,4-Difluoro-phenyl)-1-methyl-1 H-imidazol-2-yl]-ethyl}-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyrimidine;
7-Methoxy-5,8-dimethyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-
[1 ,2,4]triazolo[1 ,5-c]pyrimidine; 5,8-Dimethyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- c]pyrimidine;
2-{2-[4-(2-Methoxy-phenyl)-1-methyl-1 H-imidazol-2-yl]-ethyl}-5,7-dimethyl-
[1 ,2,4]triazolo[1 ,5-a]pyrimidine;
{5-Methyl-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- a]pyrimidin-7-yl}-methanol;
8-Ethyl-5-methyl-2-[2-(1-methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-
[1 ,2,4]triazolo[1 ,5-a]pyridine, and 5,8-Dimethoxy-2-[2-(1 -methyl-4-phenyl-1 H-imidazol-2-yl)-ethyl]-[1 ,2,4]triazolo[1 ,5- a]pyridine and pharmaceutically acceptable acid addition salts thereof.
21. A compound of any one of claims 1 to 20 as a medicament.
22. A compound of any one of claims 1 to 20 but without proviso, for use in the treatment of a neurodegenerative or psychiatric disorder, alone or in combination with one or more neuroleptic agents such as sertindole, olanzapine, risperidone, quetiapine, aripiprazole, haloperidol, clozapine, ziprasidone and osanetant, wherein the neurodegenerative disorder is selected from the group consisting of Alzheimer's disease, multi-infarct dementia, alcoholic dementia or other drug- related dementia, dementia associated with intracranial tumors or cerebral trauma, dementia associated with Huntington's disease or Parkinson's disease, or AIDS-related dementia; delirium; amnestic disorder; post-traumatic stress disorder; mental retardation; a learning disorder, for example reading disorder, mathematics disorder, or a disorder of written expression; attention- deficit/hyperactivity disorder; and age-related cognitive decline, and the psychiatric disorder is selected from the group consisting of schizophrenia, for example of the paranoid, disorganized, catatonic, undifferentiated, or residual type; schizophreniform disorder; schizoaffective disorder, for example of the delusional type or the depressive type; delusional disorder; bipolar disorder, for example bipolar I disorder, bipolar Il disorder, and cyclothymic disorder; substance-induced psychotic disorder, for example psychosis induced by alcohol, amphetamine, cannabis, cocaine, hallucinogens, inhalants, opioids, or phencyclidine; personality disorder of the paranoid type; and personality disorder of the schizoid type.
23. A compound of any one of claims 1 to 20 but without proviso, for use in the treatment of a drug addiction in a mammal, including a human, such as an alcohol, amphetamine, cocaine, or opiate addiction.
24. A compound of any one of claims 1 to 20 but without proviso, for the preparation of a medicament for use in the treatment of a drug addiction in a mammal, including a human, such as an alcohol, amphetamine, cocaine, or opiate addiction.
25. A compound of any one of claims 1 to 20 but without proviso, for the preparation of a medicament for use in the treatment of a neurodegenerative or psychiatric disorder, wherein the neurodegenerative disorder is selected from the group consisting of Alzheimer's disease, multi-infarct dementia, alcoholic dementia or other drug-related dementia, dementia associated with intracranial tumors or cerebral trauma, dementia associated with Huntington's disease or Parkinson's disease, or AIDS-related dementia; delirium; amnestic disorder; post-traumatic stress disorder; mental retardation; a learning disorder, for example reading disorder, mathematics disorder, or a disorder of written expression; attention- deficit/hyperactivity disorder; and age-related cognitive decline, and the psychiatric disorder is selected from the group consisting of schizophrenia, for example of the paranoid, disorganized, catatonic, undifferentiated, or residual type; schizophreniform disorder; schizoaffective disorder, for example of the delusional type or the depressive type; delusional disorder; bipolar disorder, for example bipolar I disorder, bipolar Il disorder, and cyclothymic disorder; substance-induced psychotic disorder, for example psychosis induced by alcohol, amphetamine, cannabis, cocaine, hallucinogens, inhalants, opioids, or phencyclidine; personality disorder of the paranoid type; and personality disorder of the schizoid type.
26. A compound for the preparation of a medicament for use in the treatment according to claim 25 wherein the treatment of psychiatric disorders comprises co-administration of a neuroleptic agent such as sertindole, olanzapine, risperidone, quetiapine, aripiprazole, haloperidol, clozapine, ziprasidone and osanetant.
27. A method of treating a subject suffering from a neurodegenerative or psychiatric disorder, wherein the neurodegenerative disorder is selected from the group consisting of Alzheimer's disease, multi -infarct dementia, alcoholic dementia or other drug-related dementia, dementia associated with intracranial tumors or cerebral trauma, dementia associated with Huntington's disease or Parkinson's disease, or AIDS-related dementia; delirium; amnestic disorder; post-traumatic stress disorder; mental retardation; a learning disorder, for example reading disorder, mathematics disorder, or a disorder of written expression; attention- deficit/hyperactivity disorder; and age-related cognitive decline, and the psychiatric disorder is selected from the group consisting of schizophrenia, for example of the paranoid, disorganized, catatonic, undifferentiated, or residual type; schizophreniform disorder; schizoaffective disorder, for example of the delusional type or the depressive type; delusional disorder; bipolar disorder, for example bipolar I disorder, bipolar Il disorder, and cyclothymic disorder; substance-induced psychotic disorder, for example psychosis induced by alcohol, amphetamine, cannabis, cocaine, hallucinogens, inhalants, opioids, or phencyclidine; personality disorder of the paranoid type; and personality disorder of the schizoid type; which method comprises administering an effective amount of a compound of any one claims 1 to 20, alone or in combination with one or more neuroleptic agents such as sertindole, olanzapine, risperidone, quetiapine, aripiprazole, haloperidol, clozapine, ziprasidone and osanetant, but without proviso.
28. A method of treating a subject suffering from a drug addiction, for example an alcohol, amphetamine, cocaine, or opiate addiction, in a mammal, including a human, which method comprises administering to said subject an amount of a compound of formula I effective in treating drug addiction.
29. A method of treating a subject suffering from a drug addiction, for example an alcohol, amphetamine, cocaine, or opiate addiction, in a mammal, including a human, which method comprises administering to said subject an amount of a compound of formula I effective in inhibiting PDE10A.
30. A pharmaceutical composition comprising a therapeutically effective amount of a compound of any one of claims 1 to 20 but without proviso, and one or more pharmaceutically acceptable carriers, diluents and excipients.
PCT/DK2009/050134 2008-06-20 2009-06-19 Novel phenylimidazole derivatives as pde10a enzyme inhibitors WO2009152825A1 (en)

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RS20140135A RS53226B (en) 2008-06-20 2009-06-19 Novel phenylimidazole derivatives as pde10a enzyme inhibitors
SI200930879T SI2318394T1 (en) 2008-06-20 2009-06-19 Novel phenylimidazole derivatives as pde10a enzyme inhibitors
CN200980132027.3A CN102124002B (en) 2008-06-20 2009-06-19 Novel phenylimidazole derivatives as pde10a enzyme inhibitors
KR1020177002859A KR20170016523A (en) 2008-06-20 2009-06-19 Novel phenylimidazole derivatives as pde10a enzyme inhibitors
EA201170061A EA018880B1 (en) 2008-06-20 2009-06-19 Novel phenylimidazole derivatives as pde10a enzyme inhibitors
PL09765461T PL2318394T3 (en) 2008-06-20 2009-06-19 Novel phenylimidazole derivatives as pde10a enzyme inhibitors
ES09765461.0T ES2456349T3 (en) 2008-06-20 2009-06-19 New phenylimidazole derivatives as PDE10A enzyme inhibitors
MX2010014136A MX2010014136A (en) 2008-06-20 2009-06-19 Novel phenylimidazole derivatives as pde10a enzyme inhibitors.
CA2728335A CA2728335C (en) 2008-06-20 2009-06-19 Novel phenylimidazole derivatives as pde10a enzyme inhibitors
KR1020177034302A KR102025487B1 (en) 2008-06-20 2009-06-19 Novel phenylimidazole derivatives as pde10a enzyme inhibitors
NZ589926A NZ589926A (en) 2008-06-20 2009-06-19 Novel phenylimidazole derivatives as pde10a enzyme inhibitors
KR1020107028654A KR101703938B1 (en) 2008-06-20 2009-06-19 Novel phenylimidazole derivatives as pde10a enzyme inhibitors
JP2011513878A JP5930714B2 (en) 2008-06-20 2009-06-19 Novel phenylimidazole derivatives as enzyme PDE10A inhibitors
UAA201015420A UA102693C2 (en) 2008-06-20 2009-06-19 Phenylimidazole derivatives as pde10a enzyme inhibitors
DK09765461.0T DK2318394T3 (en) 2008-06-20 2009-06-19 KNOWN AS PHENYLIMIDAZOLDERIVATER PDE10A enzyme inhibitors
BRPI0915383A BRPI0915383B8 (en) 2008-06-20 2009-06-19 phenylimidazole derivative compound and its pharmaceutical composition.
AU2009259209A AU2009259209B2 (en) 2008-06-20 2009-06-19 Novel phenylimidazole derivatives as PDE10A enzyme inhibitors
EP09765461.0A EP2318394B1 (en) 2008-06-20 2009-06-19 Novel phenylimidazole derivatives as pde10a enzyme inhibitors
RS20140700A RS53704B1 (en) 2008-06-20 2010-06-17 Novel phenylimidazole derivative as pde10a enzyme inhibitor
MA34464A MA33367B1 (en) 2009-06-19 2010-06-17 NOVEL PHENYLIMIDAZOLE DERIVATIVE AS INHIBITOR OF ENZYME PDE10A
NZ597203A NZ597203A (en) 2009-06-19 2010-06-17 Novel phenylimidazole derivative as pde10a enzyme inhibitor
PCT/DK2010/050147 WO2010145668A1 (en) 2009-06-19 2010-06-17 Novel phenylimidazole derivative as pde10a enzyme inhibitor
DK10725373.4T DK2443105T3 (en) 2009-06-19 2010-06-17 NEW PHENYLIMIDAZOLDERIVAT AS PDE10A enzyme inhibitor
CA2765750A CA2765750A1 (en) 2009-06-19 2010-06-17 Novel phenylimidazole derivative as pde10a enzyme inhibitor
CN201080037331.2A CN102482262B (en) 2008-06-20 2010-06-17 Phenylimidazole derivative as pde10a enzyme inhibitor
EA201270046A EA021410B1 (en) 2008-06-20 2010-06-17 Novel phenylimidazole derivative as pde10a enzyme inhibitor, pharmaceutical composition based thereon, and use thereof for treating neurodegenerative and psychiatric disorders
BRPI1015983A BRPI1015983A2 (en) 2009-06-19 2010-06-17 compound, use of the compound, method for treating a patient, and pharmaceutical composition.
SI201030841T SI2443105T1 (en) 2009-06-19 2010-06-17 Novel phenylimidazole derivative as pde10a enzyme inhibitor
SG2011089919A SG176680A1 (en) 2009-06-19 2010-06-17 Novel phenylimidazole derivative as pde10a enzyme inhibitor
US13/378,405 US8841297B2 (en) 2008-06-20 2010-06-17 Phenylimidazole derivative as PDE10A enzyme inhibitor
GEAP201012536A GEP20146100B (en) 2009-06-19 2010-06-17 Novel phenylimidazole derivative as pde10a enzyme inhibitor
MYPI2011005865A MY156785A (en) 2009-06-19 2010-06-17 Novel phenylimidazole derivative as pde10a enzyme inhibator
ES10725373.4T ES2527216T3 (en) 2009-06-19 2010-06-17 New phenylimidazole derivatives as PDE10A enzyme inhibitors
MX2011013400A MX2011013400A (en) 2009-06-19 2010-06-17 Novel phenylimidazole derivative as pde10a enzyme inhibitor.
JP2012515356A JP5768044B2 (en) 2009-06-19 2010-06-17 Novel phenylimidazole derivatives as PDE10A enzyme inhibitors
PT107253734T PT2443105E (en) 2009-06-19 2010-06-17 Novel phenylimidazole derivative as pde10a enzyme inhibitor
UAA201200499A UA106378C2 (en) 2009-06-19 2010-06-17 Phenylimidazole derivative as pde10a enzyme inhibitor
PL10725373T PL2443105T3 (en) 2009-06-19 2010-06-17 Novel phenylimidazole derivative as pde10a enzyme inhibitor
AU2010262190A AU2010262190B2 (en) 2009-06-19 2010-06-17 Novel phenylimidazole derivative as PDE10A enzyme inhibitor
EP10725373.4A EP2443105B1 (en) 2009-06-19 2010-06-17 Novel phenylimidazole derivative as pde10a enzyme inhibitor
ARP100102165A AR077144A1 (en) 2009-06-19 2010-06-18 DERIVED FROM PHENYLIMIDAZOL AS AN INHIBITOR OF THE PDE10A ENZYME, PHARMACEUTICAL COMPOSITIONS AND USE OF THE SAME IN THE TREATMENT OF NEURODEGENERATIVE AND PSYCHIATRIC DISORDERS
TW099119840A TW201100430A (en) 2009-06-19 2010-06-18 Novel phenylimidazole derivative as PDE10A enzyme inhibitor
IL209834A IL209834A (en) 2008-06-20 2010-12-07 Phenylimidazole derivatives as pde10a enzyme inhibitors
ZA2011/00109A ZA201100109B (en) 2008-06-20 2011-01-04 Novel phenylimidazole derivatives as pde10a enzyme inhibitors
TNP2011000612A TN2011000612A1 (en) 2008-06-20 2011-11-29 Novel phenylimidazole derivative as pde 10a enzyme inhibitor
IL216684A IL216684A (en) 2009-06-19 2011-11-29 5,8-dimethyl-2-[2-(1-methyl-4-phenyl-1 h-imidazol-2-yl)-ethyl]-[1,2,4]triazolo[1,5-a]pyrazine, use thereof and pharmaceutical composition comprising same
CR20110654A CR20110654A (en) 2009-06-19 2011-12-08 NEW DERIVATIVES OF PHENYLIMIDAZOL AS INHIBITORS OF THE ENZYME PDE10A
GT201100322A GT201100322A (en) 2009-06-19 2011-12-14 NEW DERIVATIVES OF PHENYLIMIDAZOL AS INHIBITORS OF THE ENZYME PDE10A
ZA2011/09271A ZA201109271B (en) 2009-06-19 2011-12-15 Novel phenylimidazole derivative as pde10a enzyme inhibitor
CL2011003205A CL2011003205A1 (en) 2008-06-20 2011-12-16 Compound 5,8-dimethyl-2- [2- (1-methyl-4-phenyl-1h-imidazol-2-yl) -ethyl] - [1,2,4] triazolo [1,5-a] pyrazine; pharmaceutical composition; and its use for the treatment of a neurodegenerative disorder, such as Alzheimer's and dementias.
DO2011000397A DOP2011000397A (en) 2009-06-19 2011-12-19 NEW DERIVATIVES OF PHENYLIMIDAZOL AS INHIBITORS OF THE ENZYME PDE10A
HK12100229.9A HK1159632A1 (en) 2008-06-20 2012-01-09 Novel phenylimidazole derivatives as pde10a enzyme inhibitors pde10a
HK12112165.0A HK1171446A1 (en) 2009-06-19 2012-11-27 Phenylimidazole derivative as pde10a enzyme inhibitor pde10a
HRP20140232AT HRP20140232T1 (en) 2008-06-20 2014-03-12 Novel phenylimidazole derivatives as pde10a enzyme inhibitors
HRP20141222AT HRP20141222T1 (en) 2009-06-19 2014-12-16 Novel phenylimidazole derivative as pde10a enzyme inhibitor
CY20141101071T CY1115971T1 (en) 2009-06-19 2014-12-19 New Imidazolium Finish Derivative as PDE10A Enzyme Inhibitor
SM201500003T SMT201500003B (en) 2009-06-19 2015-01-05 New phenylimidazole derivative as PDE10A enzyme inhibitor

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