WO2009143369A3 - Method of preparing nucleosides and analogs thereof without using chromatography - Google Patents

Method of preparing nucleosides and analogs thereof without using chromatography Download PDF

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Publication number
WO2009143369A3
WO2009143369A3 PCT/US2009/044887 US2009044887W WO2009143369A3 WO 2009143369 A3 WO2009143369 A3 WO 2009143369A3 US 2009044887 W US2009044887 W US 2009044887W WO 2009143369 A3 WO2009143369 A3 WO 2009143369A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleosides
analogs
chromatography
methods
preparing nucleosides
Prior art date
Application number
PCT/US2009/044887
Other languages
French (fr)
Other versions
WO2009143369A2 (en
Inventor
Gillermo Vasquez
Original Assignee
Isis Pharmaceuticals, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Pharmaceuticals, Inc. filed Critical Isis Pharmaceuticals, Inc.
Publication of WO2009143369A2 publication Critical patent/WO2009143369A2/en
Publication of WO2009143369A3 publication Critical patent/WO2009143369A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H11/00—Compounds containing saccharide radicals esterified by inorganic acids; Metal salts thereof
    • C07H11/04—Phosphates; Phosphites; Polyphosphates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Saccharide Compounds (AREA)

Abstract

Provided herein are methods for preparing purified modified nucleosides from reaction mixtures using precipitation as opposed to traditional chromatographic methods. The methods are particularly amenable to protected nucleosides and nucleoside phosphoramidites wherein the nucleosides can be modified.
PCT/US2009/044887 2008-05-22 2009-05-21 Method of preparing nucleosides and analogs thereof without using chromatography WO2009143369A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5525708P 2008-05-22 2008-05-22
US61/055,257 2008-05-22

Publications (2)

Publication Number Publication Date
WO2009143369A2 WO2009143369A2 (en) 2009-11-26
WO2009143369A3 true WO2009143369A3 (en) 2010-04-15

Family

ID=41279375

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/044887 WO2009143369A2 (en) 2008-05-22 2009-05-21 Method of preparing nucleosides and analogs thereof without using chromatography

Country Status (1)

Country Link
WO (1) WO2009143369A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9163239B2 (en) 2013-05-01 2015-10-20 Isis Pharmaceuticals, Inc. Compositions and methods for modulating apolipoprotein C-III expression

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013022966A1 (en) 2011-08-11 2013-02-14 Isis Pharmaceuticals, Inc. Linkage modified gapped oligomeric compounds and uses thereof
CA2916252A1 (en) 2013-06-21 2014-12-24 Isis Pharmaceuticals, Inc. Compositions and methods for modulation of target nucleic acids
US9943604B2 (en) 2013-09-20 2018-04-17 Ionis Pharmaceuticals, Inc. Targeted therapeutic nucleosides and their use
US10221416B2 (en) 2014-04-24 2019-03-05 Ionis Pharmaceuticals, Inc. Oligomeric compounds comprising alpha-beta-constrained nucleic acid
CN106232125B (en) 2014-05-01 2020-10-16 Ionis制药公司 Compositions and methods for modulating PKK expression
CN106232804B (en) 2014-05-01 2019-10-25 Ionis制药公司 For adjusting the composition and method of complement factor B expression
AU2015252841B2 (en) 2014-05-01 2020-03-19 Ionis Pharmaceuticals, Inc. Compositions and methods for modulating growth hormone receptor expression
AU2015252895B2 (en) 2014-05-01 2021-07-15 Ionis Pharmaceuticals, Inc. Compositions and methods for modulating angiopoietin-like 3 expression
CN113952353A (en) 2015-11-06 2022-01-21 Ionis制药公司 Modulation of apolipoprotein (a) expression
MA43822A (en) * 2016-03-13 2018-11-28 Wave Life Sciences Ltd COMPOSITIONS AND SYNTHESIS OF PHOSPHORAMIDITE AND OLIGONUCLEOTIDES
CN107556355B (en) * 2016-06-30 2021-10-22 上海兆维科技发展有限公司 Nucleoside diphosphite amide and preparation method thereof
EP3507367A4 (en) 2016-07-05 2020-03-25 Aduro BioTech, Inc. Locked nucleic acid cyclic dinucleotide compounds and uses thereof
EP3522898A4 (en) 2016-10-06 2020-05-27 Ionis Pharmaceuticals, Inc. Method of conjugating oligomeric compounds
WO2018098264A1 (en) 2016-11-23 2018-05-31 Wave Life Sciences Ltd. Compositions and methods for phosphoramidite and oligonucleotide synthesis

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4668777A (en) * 1981-03-27 1987-05-26 University Patents, Inc. Phosphoramidite nucleoside compounds
US20040082775A1 (en) * 2002-10-25 2004-04-29 Bruce Ross Process of purifying phosphoramidites

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4668777A (en) * 1981-03-27 1987-05-26 University Patents, Inc. Phosphoramidite nucleoside compounds
US20040082775A1 (en) * 2002-10-25 2004-04-29 Bruce Ross Process of purifying phosphoramidites

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9163239B2 (en) 2013-05-01 2015-10-20 Isis Pharmaceuticals, Inc. Compositions and methods for modulating apolipoprotein C-III expression
US9181550B2 (en) 2013-05-01 2015-11-10 Isis Pharmaceuticals, Inc. Compositions and methods for modulating apolipoprotein (a) expression

Also Published As

Publication number Publication date
WO2009143369A2 (en) 2009-11-26

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