WO2009141467A1 - Système automatisé et procédé pour l'obtention d'images totalement focalisées à l'aide de microscopes à agrandissement élevé - Google Patents
Système automatisé et procédé pour l'obtention d'images totalement focalisées à l'aide de microscopes à agrandissement élevé Download PDFInfo
- Publication number
- WO2009141467A1 WO2009141467A1 PCT/ES2009/000170 ES2009000170W WO2009141467A1 WO 2009141467 A1 WO2009141467 A1 WO 2009141467A1 ES 2009000170 W ES2009000170 W ES 2009000170W WO 2009141467 A1 WO2009141467 A1 WO 2009141467A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- image
- focused
- images
- focused images
- automated system
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/241—Devices for focusing
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/006—Optical details of the image generation focusing arrangements; selection of the plane to be imaged
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B7/00—Mountings, adjusting means, or light-tight connections, for optical elements
- G02B7/28—Systems for automatic generation of focusing signals
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T5/00—Image enhancement or restoration
- G06T5/50—Image enhancement or restoration by the use of more than one image, e.g. averaging, subtraction
Abstract
L'invention concerne un système automatisé et un procédé d'obtention d'images totalement focalisées à l'aide de microscopes à agrandissement élevé. La méthodologie et l'instrumentation pour l'obtention d'images à l'aide de microscopes à agrandissement élevé, centrées dans toute la plage focale qui nous intéresse, indépendamment de l'objectif utilisé et en maintenant totalement stable la chromaticité de l'objet observé. On utilise une méthodologie qui permet d'obtenir des images partiellement focalisées à différentes distances objectif-objet pour ensuite extraire la partie focalisée de chaque image et générer par accumulation, l'image focalisée globale de l'objet. L'invention peut être appliquée à l'automatisation des microscopes optiques monoculaires, binoculaires ou trioculaires pour obtenir des images optiques parfaitement focalisées et avec toute la plage chromatique.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200801517A ES2338197B2 (es) | 2008-05-23 | 2008-05-23 | Sistema automatizado y procedimiento para la obtencion de imagenes totalmente focalizadas con microscopios de elevada magnificacion. |
| ESP200801517 | 2008-05-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2009141467A1 true WO2009141467A1 (fr) | 2009-11-26 |
Family
ID=41339801
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/ES2009/000170 WO2009141467A1 (fr) | 2008-05-23 | 2009-03-26 | Système automatisé et procédé pour l'obtention d'images totalement focalisées à l'aide de microscopes à agrandissement élevé |
Country Status (2)
| Country | Link |
|---|---|
| ES (1) | ES2338197B2 (fr) |
| WO (1) | WO2009141467A1 (fr) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4661986A (en) * | 1983-06-27 | 1987-04-28 | Rca Corporation | Depth-of-focus imaging process method |
| US20020181762A1 (en) * | 2001-05-30 | 2002-12-05 | Mitutoyo Corporation | Systems and methods for constructing an image having an extended depth of field |
| EP1336888A1 (fr) * | 2002-02-13 | 2003-08-20 | Fairfield Imaging Ltd. | Système d'imagerie microscopique et méthode d'acquisition de données |
| US20060038144A1 (en) * | 2004-08-23 | 2006-02-23 | Maddison John R | Method and apparatus for providing optimal images of a microscope specimen |
-
2008
- 2008-05-23 ES ES200801517A patent/ES2338197B2/es active Active
-
2009
- 2009-03-26 WO PCT/ES2009/000170 patent/WO2009141467A1/fr active Application Filing
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4661986A (en) * | 1983-06-27 | 1987-04-28 | Rca Corporation | Depth-of-focus imaging process method |
| US20020181762A1 (en) * | 2001-05-30 | 2002-12-05 | Mitutoyo Corporation | Systems and methods for constructing an image having an extended depth of field |
| EP1336888A1 (fr) * | 2002-02-13 | 2003-08-20 | Fairfield Imaging Ltd. | Système d'imagerie microscopique et méthode d'acquisition de données |
| US20060038144A1 (en) * | 2004-08-23 | 2006-02-23 | Maddison John R | Method and apparatus for providing optimal images of a microscope specimen |
Also Published As
| Publication number | Publication date |
|---|---|
| ES2338197A1 (es) | 2010-05-04 |
| ES2338197B2 (es) | 2012-10-15 |
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