WO2009140701A3 - Administration dans des cellules à l’aide de lasers à impulsions ultracourtes - Google Patents

Administration dans des cellules à l’aide de lasers à impulsions ultracourtes Download PDF

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Publication number
WO2009140701A3
WO2009140701A3 PCT/US2009/044415 US2009044415W WO2009140701A3 WO 2009140701 A3 WO2009140701 A3 WO 2009140701A3 US 2009044415 W US2009044415 W US 2009044415W WO 2009140701 A3 WO2009140701 A3 WO 2009140701A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
ultra
short pulse
delivery
pulse lasers
Prior art date
Application number
PCT/US2009/044415
Other languages
English (en)
Other versions
WO2009140701A2 (fr
Inventor
Helge Zieler
Dave Thomas Bullis
David Light
Original Assignee
Synthetic Genomics, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synthetic Genomics, Inc. filed Critical Synthetic Genomics, Inc.
Publication of WO2009140701A2 publication Critical patent/WO2009140701A2/fr
Publication of WO2009140701A3 publication Critical patent/WO2009140701A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention se rapporte à l'utilisation de lasers à impulsions ultracourtes (USP) pour administrer des macromolécules dans des cellules. L'invention concerne des procédés pour introduire des macromolécules telles que des protéines, des peptides, des acides aminés, des acides nucléiques, un ADN, un ARN, des oligonucléotides, des lipides, des hydrates de carbone ou toutes combinaisons de ceux-ci dans des cellules ayant des parois de cellule, telles que des bactéries, des archées, des champignons, de la levure, des algues et des cellules végétales, en utilisant un laser USP ultraviolet.
PCT/US2009/044415 2008-05-16 2009-05-18 Administration dans des cellules à l’aide de lasers à impulsions ultracourtes WO2009140701A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5407008P 2008-05-16 2008-05-16
US61/054,070 2008-05-16

Publications (2)

Publication Number Publication Date
WO2009140701A2 WO2009140701A2 (fr) 2009-11-19
WO2009140701A3 true WO2009140701A3 (fr) 2010-01-07

Family

ID=41319390

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/044415 WO2009140701A2 (fr) 2008-05-16 2009-05-18 Administration dans des cellules à l’aide de lasers à impulsions ultracourtes

Country Status (1)

Country Link
WO (1) WO2009140701A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2655647A4 (fr) 2010-12-23 2016-04-27 Exxonmobil Res & Eng Co Culture d'un microorganisme dans un milieu contenant un niveau élevé d'une source de contre-ion carboxylate
WO2012135668A1 (fr) 2011-03-31 2012-10-04 Exxonmobil Research And Engineering Company Ciblage des voies métaboliques par surexpression du facteur de transcription
CN103635571B (zh) 2011-06-30 2016-12-14 艾克森美孚研究与工程公司 调控毒素和抗毒素基因用于生物防范
US8778682B2 (en) 2011-08-30 2014-07-15 General Electric Company Optical based delivery of exogenous molecules to cells
AU2012316749B2 (en) 2011-09-27 2017-06-29 Exxonmobil Research And Engineering Company Acyl-ACP wax ester synthases
US8962299B2 (en) 2012-02-29 2015-02-24 Exxonmobil Research And Engineering Company Four-gene pathway for wax ester synthesis
EP3334831A1 (fr) 2015-08-13 2018-06-20 Forrest Innovations Ltd. Formulations et compositions d'administration d'acides nucléiques à des cellules végétales
US11058575B2 (en) 2017-10-25 2021-07-13 École Polytechnique Method and system for delivering exogenous biomolecules into the eye and method for forming pores into target cells of an eye

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6060315A (en) * 1997-12-01 2000-05-09 Lockheed Martin Energy Research Corporation Method for facilitating the introduction of material into cells
US20020115219A1 (en) * 2000-12-25 2002-08-22 Osaka University Method for introducing foreign matters into living cells
US20020169138A1 (en) * 1997-10-24 2002-11-14 Southern Research Institute Delivery vehicles for bioactive agents and uses thereof
US20040102606A1 (en) * 2001-04-24 2004-05-27 Danuta Balicki Histone H2A -derived peptides useful in gene delivery
US20040203099A1 (en) * 2001-05-25 2004-10-14 Solari Roberto Celeste Ercole Conjugate of a transport protein and a protein for modulation of notch signalling
US20050095578A1 (en) * 2003-10-31 2005-05-05 Koller Manfred R. Method and apparatus for cell permeabilization
US20060141624A1 (en) * 2002-05-23 2006-06-29 Karsten Koenig Method for transferring molecules in vital cells by means of laser beams and arrangement for carrying out said method
US20070254358A1 (en) * 2001-03-27 2007-11-01 Invitrogen Corporation Culture medium for cell growth and transfection

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020169138A1 (en) * 1997-10-24 2002-11-14 Southern Research Institute Delivery vehicles for bioactive agents and uses thereof
US6060315A (en) * 1997-12-01 2000-05-09 Lockheed Martin Energy Research Corporation Method for facilitating the introduction of material into cells
US20020115219A1 (en) * 2000-12-25 2002-08-22 Osaka University Method for introducing foreign matters into living cells
US20070254358A1 (en) * 2001-03-27 2007-11-01 Invitrogen Corporation Culture medium for cell growth and transfection
US20040102606A1 (en) * 2001-04-24 2004-05-27 Danuta Balicki Histone H2A -derived peptides useful in gene delivery
US20040203099A1 (en) * 2001-05-25 2004-10-14 Solari Roberto Celeste Ercole Conjugate of a transport protein and a protein for modulation of notch signalling
US20060141624A1 (en) * 2002-05-23 2006-06-29 Karsten Koenig Method for transferring molecules in vital cells by means of laser beams and arrangement for carrying out said method
US20050095578A1 (en) * 2003-10-31 2005-05-05 Koller Manfred R. Method and apparatus for cell permeabilization

Also Published As

Publication number Publication date
WO2009140701A2 (fr) 2009-11-19

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