WO2009139881A3 - Compositions and methods for growing embryonic stem cells - Google Patents
Compositions and methods for growing embryonic stem cells Download PDFInfo
- Publication number
- WO2009139881A3 WO2009139881A3 PCT/US2009/002983 US2009002983W WO2009139881A3 WO 2009139881 A3 WO2009139881 A3 WO 2009139881A3 US 2009002983 W US2009002983 W US 2009002983W WO 2009139881 A3 WO2009139881 A3 WO 2009139881A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- stem cells
- methods
- embryonic stem
- compositions
- growing embryonic
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
Abstract
Methods for deriving and cultivating human embryonic stem (ES) cells and maintaining their pluripotency in culture is provided by utilizing human umbilical cord stem cells or secreted proteins obtained from the culture medium of human umbilical cord stem cells.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US7173308P | 2008-05-14 | 2008-05-14 | |
US61/071,733 | 2008-05-14 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009139881A2 WO2009139881A2 (en) | 2009-11-19 |
WO2009139881A3 true WO2009139881A3 (en) | 2010-01-07 |
Family
ID=41319212
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2009/002983 WO2009139881A2 (en) | 2008-05-14 | 2009-05-14 | Compositions and methods for growing embryonic stem cells |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2009139881A2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102250835A (en) * | 2011-07-13 | 2011-11-23 | 北京大学第三医院 | Method for culturing human embryo stem cell by using umbilical cord source mesenchymal stem cell |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030152558A1 (en) * | 2001-11-09 | 2003-08-14 | Christopher Luft | Methods and compositions for the use of stromal cells to support embryonic and adult stem cells |
US20070077652A1 (en) * | 2004-09-16 | 2007-04-05 | Tony Peled | Methods of ex vivo progenitor and stem cell expansion by co-culture with mesenchymal cells |
WO2008008550A2 (en) * | 2006-07-14 | 2008-01-17 | National Stem Cell Inc. | Compositions and methods for growing human embryonic stem cells |
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2009
- 2009-05-14 WO PCT/US2009/002983 patent/WO2009139881A2/en active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030152558A1 (en) * | 2001-11-09 | 2003-08-14 | Christopher Luft | Methods and compositions for the use of stromal cells to support embryonic and adult stem cells |
US20070077652A1 (en) * | 2004-09-16 | 2007-04-05 | Tony Peled | Methods of ex vivo progenitor and stem cell expansion by co-culture with mesenchymal cells |
WO2008008550A2 (en) * | 2006-07-14 | 2008-01-17 | National Stem Cell Inc. | Compositions and methods for growing human embryonic stem cells |
Non-Patent Citations (2)
Title |
---|
BIEBACK ET AL.: "Critical Parameters for the isolation of Mesenchymal Stem Cells from Umbilical Cord Blood.", STEM CELLS, vol. 22, 2004, pages 625 - 634 * |
ERICES ET AL.: "Mesenchymal progenitor cells in human umbilical cord blood.", BRITISH JOURNAL OF HAEMATOLOGY, vol. 109, 2000, pages 235 - 242 * |
Also Published As
Publication number | Publication date |
---|---|
WO2009139881A2 (en) | 2009-11-19 |
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