WO2009139881A3 - Compositions and methods for growing embryonic stem cells - Google Patents

Compositions and methods for growing embryonic stem cells Download PDF

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Publication number
WO2009139881A3
WO2009139881A3 PCT/US2009/002983 US2009002983W WO2009139881A3 WO 2009139881 A3 WO2009139881 A3 WO 2009139881A3 US 2009002983 W US2009002983 W US 2009002983W WO 2009139881 A3 WO2009139881 A3 WO 2009139881A3
Authority
WO
WIPO (PCT)
Prior art keywords
stem cells
methods
embryonic stem
compositions
growing embryonic
Prior art date
Application number
PCT/US2009/002983
Other languages
French (fr)
Other versions
WO2009139881A2 (en
Inventor
Michael Cohen
Zhan Xiangcan
Michael Shamblott
Original Assignee
Proteonomix, Inc.
The Johns Hopkins University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Proteonomix, Inc., The Johns Hopkins University filed Critical Proteonomix, Inc.
Publication of WO2009139881A2 publication Critical patent/WO2009139881A2/en
Publication of WO2009139881A3 publication Critical patent/WO2009139881A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Reproductive Health (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Methods for deriving and cultivating human embryonic stem (ES) cells and maintaining their pluripotency in culture is provided by utilizing human umbilical cord stem cells or secreted proteins obtained from the culture medium of human umbilical cord stem cells.
PCT/US2009/002983 2008-05-14 2009-05-14 Compositions and methods for growing embryonic stem cells WO2009139881A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7173308P 2008-05-14 2008-05-14
US61/071,733 2008-05-14

Publications (2)

Publication Number Publication Date
WO2009139881A2 WO2009139881A2 (en) 2009-11-19
WO2009139881A3 true WO2009139881A3 (en) 2010-01-07

Family

ID=41319212

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/002983 WO2009139881A2 (en) 2008-05-14 2009-05-14 Compositions and methods for growing embryonic stem cells

Country Status (1)

Country Link
WO (1) WO2009139881A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102250835A (en) * 2011-07-13 2011-11-23 北京大学第三医院 Method for culturing human embryo stem cell by using umbilical cord source mesenchymal stem cell

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030152558A1 (en) * 2001-11-09 2003-08-14 Christopher Luft Methods and compositions for the use of stromal cells to support embryonic and adult stem cells
US20070077652A1 (en) * 2004-09-16 2007-04-05 Tony Peled Methods of ex vivo progenitor and stem cell expansion by co-culture with mesenchymal cells
WO2008008550A2 (en) * 2006-07-14 2008-01-17 National Stem Cell Inc. Compositions and methods for growing human embryonic stem cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030152558A1 (en) * 2001-11-09 2003-08-14 Christopher Luft Methods and compositions for the use of stromal cells to support embryonic and adult stem cells
US20070077652A1 (en) * 2004-09-16 2007-04-05 Tony Peled Methods of ex vivo progenitor and stem cell expansion by co-culture with mesenchymal cells
WO2008008550A2 (en) * 2006-07-14 2008-01-17 National Stem Cell Inc. Compositions and methods for growing human embryonic stem cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BIEBACK ET AL.: "Critical Parameters for the isolation of Mesenchymal Stem Cells from Umbilical Cord Blood.", STEM CELLS, vol. 22, 2004, pages 625 - 634 *
ERICES ET AL.: "Mesenchymal progenitor cells in human umbilical cord blood.", BRITISH JOURNAL OF HAEMATOLOGY, vol. 109, 2000, pages 235 - 242 *

Also Published As

Publication number Publication date
WO2009139881A2 (en) 2009-11-19

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