WO2009133038A1 - Polypeptide de modification de glucide et ses utilisations - Google Patents

Polypeptide de modification de glucide et ses utilisations Download PDF

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Publication number
WO2009133038A1
WO2009133038A1 PCT/EP2009/055001 EP2009055001W WO2009133038A1 WO 2009133038 A1 WO2009133038 A1 WO 2009133038A1 EP 2009055001 W EP2009055001 W EP 2009055001W WO 2009133038 A1 WO2009133038 A1 WO 2009133038A1
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptide
sequence
seq
plant
protein
Prior art date
Application number
PCT/EP2009/055001
Other languages
English (en)
Inventor
Wilbert Herman Marie Heijne
Margot Elisabeth Francoise Schooneveld-Bergmans
Van Den Marco Alexander Berg
Johannes Andries Roubos
Original Assignee
Dsm Ip Assets B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets B.V. filed Critical Dsm Ip Assets B.V.
Publication of WO2009133038A1 publication Critical patent/WO2009133038A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C11D2111/12—
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

Abstract

L'invention concerne un polypeptide présentant une activité de modification de matière glucidique autre que l'amidon, qui comprend une séquence d'acides aminés représentée dans SEQ ID NO: 3 ou une séquence d'acides aminés codée par la séquence nucléotidique de SEQ ID NO: 1 et/ou SEQ ID NO: 2, ou un variant ou un fragment de l'une ou l'autre des séquences. L'invention concerne également la séquence de codage pleine longueur du nouveau gène ainsi que la séquence d'acides aminés de la protéine fonctionnelle pleine longueur et d'équivalents fonctionnels du gène ou de la séquence d'acides aminés. L'invention concerne en outre des procédés d'utilisation du polypeptide dans des processus industriels. L'invention concerne enfin des cellules transformées par le polynucléotide de l'invention approprié pour produire desdites protéines.
PCT/EP2009/055001 2008-04-29 2009-04-24 Polypeptide de modification de glucide et ses utilisations WO2009133038A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08075397.3 2008-04-29
EP08075397 2008-04-29

Publications (1)

Publication Number Publication Date
WO2009133038A1 true WO2009133038A1 (fr) 2009-11-05

Family

ID=40897661

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/055001 WO2009133038A1 (fr) 2008-04-29 2009-04-24 Polypeptide de modification de glucide et ses utilisations

Country Status (1)

Country Link
WO (1) WO2009133038A1 (fr)

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DATABASE UniProt [online] 16 December 2008 (2008-12-16), "SubName: Full=Pc20g07340 protein; Flags: Precursor;", XP002539900, retrieved from EBI accession no. UNIPROT:B6HDE0 Database accession no. B6HDE0 *
DATABASE UniProt [online] 23 January 2007 (2007-01-23), "SubName: Full=Putative uncharacterized protein;", XP002539899, retrieved from EBI accession no. UNIPROT:A1CC76 Database accession no. A1CC76 *
FEDOROVA NATALIE D ET AL: "Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus", PLOS GENETICS, PUBLIC LIBRARY OF SCIENCE, SAN FRANCISCO, CA, US, vol. 4, no. 4, 1 April 2008 (2008-04-01), pages e1000046.1 - e1000046., XP002519164, ISSN: 1553-7390 *
HOU YUNHUA ET AL: "Cloning, sequencing and expression analysis of the first cellulase gene encoding cellobiohydrolase 1 from a cold-adaptive Penicillium chrysogenum FS010", ACTA BIOCHIMICA ET BIOPHYSICA SINICA, vol. 39, no. 2, 2007, pages 101 - 107, XP009118909, ISSN: 1672-9145, [retrieved on 20070212] *
KARBOUNE SALWA ET AL: "Characterization of selected cellulolytic activities of multi-enzymatic complex system from Penicillium funiculosum", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,, vol. 56, no. 3, 1 February 2008 (2008-02-01), pages 903 - 909, XP002534038 *
NUERO O M ET AL: "Enzymes for animal feeding from Penicillium chrysogenum mycelial wastes from penicillin manufacture", LETTERS IN APPLIED MICROBIOLOGY, vol. 34, no. 6, 2002, pages 413 - 416, XP002539898, ISSN: 0266-8254 *
VAN DEN BERG MARCO A ET AL: "Genome sequencing and analysis of the filamentous fungus Penicillium chrysogenum", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 26, no. 10, 1 October 2008 (2008-10-01), pages 1161 - 1168, XP002519165, ISSN: 1087-0156 *

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