WO2009126463A3 - Filamentous fungi with inactivated protease genes for altered protein production - Google Patents
Filamentous fungi with inactivated protease genes for altered protein production Download PDFInfo
- Publication number
- WO2009126463A3 WO2009126463A3 PCT/US2009/038626 US2009038626W WO2009126463A3 WO 2009126463 A3 WO2009126463 A3 WO 2009126463A3 US 2009038626 W US2009038626 W US 2009038626W WO 2009126463 A3 WO2009126463 A3 WO 2009126463A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- protein production
- filamentous fungi
- altered protein
- protease genes
- inactivated protease
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Abstract
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2009801122959A CN102099460A (en) | 2008-04-08 | 2009-03-27 | Filamentous fungi with inactivated protease genes for altered protein production |
EP09729753A EP2262888A2 (en) | 2008-04-08 | 2009-03-27 | Filamentous fungi with inactivated protease genes for altered protein production |
CA2720827A CA2720827A1 (en) | 2008-04-08 | 2009-03-27 | Filamentous fungi with inactivated protease genes for altered protein production |
JP2011504054A JP2011517943A (en) | 2008-04-08 | 2009-03-27 | Filamentous fungi with inactivated protease genes for the production of modified proteins |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US4328408P | 2008-04-08 | 2008-04-08 | |
US61/043,284 | 2008-04-08 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009126463A2 WO2009126463A2 (en) | 2009-10-15 |
WO2009126463A3 true WO2009126463A3 (en) | 2010-01-14 |
Family
ID=40848716
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2009/038626 WO2009126463A2 (en) | 2008-04-08 | 2009-03-27 | Filamentous fungi with inactivated protease genes for altered protein production |
Country Status (6)
Country | Link |
---|---|
US (2) | US20090253173A1 (en) |
EP (1) | EP2262888A2 (en) |
JP (1) | JP2011517943A (en) |
CN (1) | CN102099460A (en) |
CA (1) | CA2720827A1 (en) |
WO (1) | WO2009126463A2 (en) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012048334A2 (en) * | 2010-10-08 | 2012-04-12 | Dyadic International (Usa) Inc. | Novel fungal proteases |
EP2760997A4 (en) | 2011-09-30 | 2015-02-11 | Codexis Inc | Fungal proteases |
BR112014016587A2 (en) | 2012-01-05 | 2020-10-27 | Novartis Ag | protease-deficient filamentous fungal cells and methods of using them |
WO2013174927A1 (en) | 2012-05-23 | 2013-11-28 | Novartis International Pharmaceutical Limited | Production of fucosylated glycoproteins |
WO2013181647A2 (en) | 2012-06-01 | 2013-12-05 | Danisco Us Inc. | Compositions and methods of producing isoprene and/or industrrial bio-products using anaerobic microorganisms |
CA2916594C (en) * | 2013-07-04 | 2020-03-10 | Novartis Ag | O-mannosyltransferase deficient filamentous fungal cells and methods of use thereof |
WO2015004241A2 (en) | 2013-07-10 | 2015-01-15 | Novartis Ag | Multiple proteases deficient filamentous fungal cells and methods of use thereof |
WO2015054507A1 (en) * | 2013-10-10 | 2015-04-16 | Pronutria, Inc. | Nutritive polypeptide production systems, and methods of manufacture and use thereof |
EP3172333B1 (en) | 2014-07-21 | 2020-05-13 | Glykos Finland Oy | Production of glycoproteins with mammalian-like n-glycans in filamentous fungi |
CN104846090B (en) * | 2015-05-12 | 2018-08-17 | 海南大学 | Genetic of fungi screening technique based on marker gene |
KR102593668B1 (en) * | 2016-10-04 | 2023-10-24 | 다니스코 유에스 인크. | Protein production in filamentous fungal cells in the absence of inducing substrates |
CN110564625B (en) * | 2019-08-13 | 2023-01-24 | 内蒙古世洪农业科技有限公司 | Saline-alkali resistant aspergillus flavus and separation method and application thereof |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997022705A1 (en) * | 1995-12-15 | 1997-06-26 | Novo Nordisk A/S | A FUNGUNS WHEREIN THE areA, pepC AND/OR pepE GENES HAVE BEEN INACTIVATED |
US5840570A (en) * | 1988-07-01 | 1998-11-24 | Berka; Randy M. | Aspartic proteinase deficient filamentous fungi |
WO1999002705A1 (en) * | 1997-07-05 | 1999-01-21 | Societe Des Produits Nestle S.A. | CLONING OF THE PROLYL-DIPEPTIDYL-PEPTIDASE FROM $i(ASPERGILLUS ORYZAE) |
WO2006110677A2 (en) * | 2005-04-12 | 2006-10-19 | Genencor International, Inc. | Gene inactivated mutants with altered protein production |
WO2007062936A2 (en) * | 2005-11-29 | 2007-06-07 | Dsm Ip Assets B.V. | Dna binding site of a transcriptional activator useful in gene expression |
Family Cites Families (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4760025A (en) * | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
DE69133612D1 (en) * | 1990-12-10 | 2009-04-02 | Genencor Int | Improved cellulose saccharification by cloning and amplification of the Trichoderma beta-glucosidase gene |
US5674728A (en) * | 1993-11-03 | 1997-10-07 | Novartis Corporation | Aspergillus niger vacuolar aspartyl protease |
US5861271A (en) * | 1993-12-17 | 1999-01-19 | Fowler; Timothy | Cellulase enzymes and systems for their expressions |
US5594119A (en) * | 1994-09-20 | 1997-01-14 | Novo Nordisk A/S | Gene encoding carboxypeptidase of aspergillus niger |
DE69631127T2 (en) * | 1995-05-18 | 2004-07-08 | Genencor International, Inc., Palo Alto | GLYCOSYL TRANSFERASE EXPRESSION IN ASPERGILLUS |
JPH11501217A (en) * | 1995-08-03 | 1999-02-02 | ギスト ブロカデス ベスローテン フェンノートシャップ | Use of the homologous amdS gene as a selectable marker |
US6187577B1 (en) * | 1997-11-19 | 2001-02-13 | Genecor International, Inc. | Cellulase producing Actinomycetes cellulase produced therefrom and method of producing same |
US6268328B1 (en) * | 1998-12-18 | 2001-07-31 | Genencor International, Inc. | Variant EGIII-like cellulase compositions |
AU2001234443A1 (en) * | 2000-01-13 | 2001-07-24 | Novozymes Biotech, Inc. | Methods for producing a polypeptide using a crippled translational initiator sequence |
US7323327B2 (en) * | 2001-02-23 | 2008-01-29 | Dsm Ip Assets B.V. | Genes encoding novel proteolytic enzymes |
US20040014955A1 (en) * | 2001-12-17 | 2004-01-22 | Carlos Zamudio | Identification of essential genes of cryptococcus neoformans and methods of use |
AU2002358179A1 (en) * | 2001-12-21 | 2003-07-09 | Dsm Ip Assets B.V. | New rennets |
AU2003263163A1 (en) * | 2002-09-20 | 2004-04-08 | Cym1P A/S | Methods for increasing the production of a recombinant polypeptide from a host cell |
JP4050712B2 (en) * | 2004-02-27 | 2008-02-20 | 独立行政法人科学技術振興機構 | Method for homologous recombination |
JP2008507294A (en) * | 2004-07-26 | 2008-03-13 | ダウ グローバル テクノロジーズ インコーポレイティド | Method for improved protein expression by strain genetic manipulation |
US20060211089A1 (en) * | 2005-03-16 | 2006-09-21 | Novozymes A/S | Expression of defensins in filamentous fungi |
EP1930428B1 (en) * | 2005-08-03 | 2010-12-01 | Asahi Glass Company, Limited | transformed yeast host cells AND METHOD OF PRODUCING FOREIGN PROTEIN |
US8216844B2 (en) * | 2006-08-29 | 2012-07-10 | Danisco Us Inc. | Compositions and methods for improved protein production |
JP4998873B2 (en) * | 2006-08-30 | 2012-08-15 | 独立行政法人酒類総合研究所 | How to make filamentous fungi that produce spores with low viability |
-
2009
- 2009-03-26 US US12/412,297 patent/US20090253173A1/en not_active Abandoned
- 2009-03-27 EP EP09729753A patent/EP2262888A2/en not_active Withdrawn
- 2009-03-27 JP JP2011504054A patent/JP2011517943A/en active Pending
- 2009-03-27 WO PCT/US2009/038626 patent/WO2009126463A2/en active Application Filing
- 2009-03-27 CN CN2009801122959A patent/CN102099460A/en active Pending
- 2009-03-27 CA CA2720827A patent/CA2720827A1/en not_active Abandoned
-
2013
- 2013-02-28 US US13/780,860 patent/US20130266984A1/en not_active Abandoned
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5840570A (en) * | 1988-07-01 | 1998-11-24 | Berka; Randy M. | Aspartic proteinase deficient filamentous fungi |
US6509171B1 (en) * | 1988-07-01 | 2003-01-21 | Genencor International, Inc. | Aspartic proteinase deficient filamentous fungi |
WO1997022705A1 (en) * | 1995-12-15 | 1997-06-26 | Novo Nordisk A/S | A FUNGUNS WHEREIN THE areA, pepC AND/OR pepE GENES HAVE BEEN INACTIVATED |
WO1999002705A1 (en) * | 1997-07-05 | 1999-01-21 | Societe Des Produits Nestle S.A. | CLONING OF THE PROLYL-DIPEPTIDYL-PEPTIDASE FROM $i(ASPERGILLUS ORYZAE) |
WO2006110677A2 (en) * | 2005-04-12 | 2006-10-19 | Genencor International, Inc. | Gene inactivated mutants with altered protein production |
WO2007062936A2 (en) * | 2005-11-29 | 2007-06-07 | Dsm Ip Assets B.V. | Dna binding site of a transcriptional activator useful in gene expression |
Non-Patent Citations (6)
Title |
---|
BARTKEVICIUTE D ET AL: "Disruption of the MNN10 gene enhances protein secretion in Kluyveromyces lactis and Saccharomyces cerevisiae", FEMS YEAST RESEARCH, ELSEVIER SCIENCE, TOKYO, NL, vol. 4, no. 8, 1 September 2004 (2004-09-01), pages 833 - 840, XP004533627, ISSN: 1567-1356 * |
HOMBERGH VAN DEN J P T W ET AL: "DISRUPTION OF THREE ACID PROTEASES IN ASPERGILLUS NIGER EFFECTS ON PROTEASE SPECTRUM, INTRACELLULAR PROTEOLYSIS, AND DEGRADATION OF TARGET PROTEINS", EUROPEAN JOURNAL OF BIOCHEMISTRY, BLACKWELL PUBLISHING, BERLIN, DE, vol. 247, no. 2, 1 January 1997 (1997-01-01), pages 605 - 613, XP001153668, ISSN: 0014-2956 * |
OKA TAKUJI ET AL: "Molecular characterization of protein O-mannosyltransferase and its involvement in cell-wall synthesis in Aspergillus nidulans", MICROBIOLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, GB, vol. 150, no. Part 6, 1 June 2004 (2004-06-01), pages 1973 - 1982, XP002399299, ISSN: 1350-0872 * |
SUELMANN RUEDIGER ET AL: "Increased nuclear traffic chaos in hyphae of Aspergillus nidulans: Molecular characterization of apsB and in vivo observation of nuclear behaviour", MOLECULAR MICROBIOLOGY, vol. 30, no. 4, November 1998 (1998-11-01), pages 831 - 842, XP002537754, ISSN: 0950-382X * |
VAN DEN HOMBERGH J P T W ET AL: "Aspergillus as a host for heterologous protein production: the problem of proteases", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 15, no. 7, 1 July 1997 (1997-07-01), pages 256 - 263, XP004092682, ISSN: 0167-7799 * |
WANG ET AL: "Isolation of four pepsin-like protease genes from Aspergillus niger and analysis of the effect of disruptions on heterologous laccase expression", FUNGAL GENETICS AND BIOLOGY, SAN DIEGO, CA, US, vol. 45, no. 1, 15 November 2007 (2007-11-15), pages 17 - 27, XP022346803, ISSN: 1087-1845 * |
Also Published As
Publication number | Publication date |
---|---|
EP2262888A2 (en) | 2010-12-22 |
US20090253173A1 (en) | 2009-10-08 |
US20130266984A1 (en) | 2013-10-10 |
CN102099460A (en) | 2011-06-15 |
CA2720827A1 (en) | 2009-10-15 |
JP2011517943A (en) | 2011-06-23 |
WO2009126463A2 (en) | 2009-10-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2009126463A3 (en) | Filamentous fungi with inactivated protease genes for altered protein production | |
WO2014205192A3 (en) | Targeted integration | |
WO2007140339A8 (en) | Production of isoprenoids | |
CA3033327A1 (en) | Programmable cas9-recombinase fusion proteins and uses thereof | |
WO2008153029A1 (en) | Method for expression of specific gene | |
WO2010039889A3 (en) | Methods for using positively and negatively selectable genes in a filamentous fungal cell | |
WO2006073727A3 (en) | Recombinant dna constructs and methods for controlling gene expression | |
WO2012058494A3 (en) | Isoprene synthase variants for improved production of isoprene | |
WO2009042070A3 (en) | Production of isoprenoids | |
WO2008104890A3 (en) | Compositions and methods for producing apolipoprotein | |
WO2008154695A8 (en) | Method for improving stress resistance in plants and materials therefor | |
WO2005090552A3 (en) | Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast | |
WO2007146706A3 (en) | Modified dicamba monooxygenase enzyme and methods of its use | |
WO2010107303A3 (en) | Chrysosporium lucknowense protein production system | |
WO2008153903A3 (en) | Heterologous and homologous cellulase expression system | |
WO2006110677A3 (en) | Gene inactivated mutants with altered protein production | |
WO2012009272A3 (en) | Pentose fermentation by a recombinant microorganism | |
WO2010135836A8 (en) | Novel beta-glucosidase enzymes | |
WO2008071726A3 (en) | Pathogen inducible plant trehalose-6-phophate phophatase gene promoters and regulatory elements | |
WO2009158627A3 (en) | Cellulosic protein expression in yeast | |
WO2008065200A8 (en) | Dnase expression in recombinant host cells | |
WO2012168493A3 (en) | Baculoviral dna elements for the expression of recombinant proteins in a host insect | |
WO2011151512A3 (en) | Method for protein production in filamentous fungi | |
MX2010009071A (en) | Isolated peptides having phospholipase inhibitory activity. | |
WO2010127219A3 (en) | Altering enzyme balance through fermentation conditions |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WWE | Wipo information: entry into national phase |
Ref document number: 200980112295.9 Country of ref document: CN |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 09729753 Country of ref document: EP Kind code of ref document: A2 |
|
WWE | Wipo information: entry into national phase |
Ref document number: 6303/DELNP/2010 Country of ref document: IN |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2009729753 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2720827 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2011504054 Country of ref document: JP |
|
NENP | Non-entry into the national phase |
Ref country code: DE |