WO2009123298A1 - Method for production of flavoring material for imparting aged flavor - Google Patents

Method for production of flavoring material for imparting aged flavor Download PDF

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Publication number
WO2009123298A1
WO2009123298A1 PCT/JP2009/056923 JP2009056923W WO2009123298A1 WO 2009123298 A1 WO2009123298 A1 WO 2009123298A1 JP 2009056923 W JP2009056923 W JP 2009056923W WO 2009123298 A1 WO2009123298 A1 WO 2009123298A1
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WIPO (PCT)
Prior art keywords
seasoning
source
hours
less
amino acid
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PCT/JP2009/056923
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French (fr)
Japanese (ja)
Inventor
長崎浩明
廣瀬貴子
高木伸昌
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味の素株式会社
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Application filed by 味の素株式会社 filed Critical 味の素株式会社
Priority to CN2009801125340A priority Critical patent/CN101983017B/en
Priority to JP2010505990A priority patent/JP5678658B2/en
Publication of WO2009123298A1 publication Critical patent/WO2009123298A1/en

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/56Flavouring or bittering agents
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • A23L27/21Synthetic spices, flavouring agents or condiments containing amino acids
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • A23L27/21Synthetic spices, flavouring agents or condiments containing amino acids
    • A23L27/215Synthetic spices, flavouring agents or condiments containing amino acids heated in the presence of reducing sugars, e.g. Maillard's non-enzymatic browning

Definitions

  • the present invention relates to a method for producing a seasoning capable of imparting a high-titer and high-quality ripening feeling to food and drink and use of the seasoning.
  • seasonings that give a rich taste have been widely used for the purpose of full-fledged seasoning of food and drink. From a full-fledged point of view, processed foods manufactured in a short period of time are desired to have sufficient aging. Proteins that can be given a general rich taste, such as protein hydrolysates, animal extracts and yeast extracts, and seasonings obtained by heat reaction of these with sugars, etc., are used in various foods.
  • a seasoning technology having a good mouth-to-mouth flavor flavor is known, which is characterized in that a sugar extract or an amino acid as necessary is added to a yeast extract containing a sulfur-containing compound and heated.
  • a seasoning technology having a good flavor-like flavor characterized by heating an aqueous solution containing 5′-nucleotide-containing yeast extract, glutathione yeast extract, monosaccharide, dextrin and salt, etc. Is known (see Patent Document 2).
  • Patent Document 2
  • Patent Document 5
  • Patent Document 1 Japanese Patent Laid-Open No. 2 0 0 5-2 8 7 4 3 3 Disclosure of Invention
  • Patent Document 1 described above was aimed at obtaining a seasoning having a good roasted meat flavor-like flavor rather than imparting a sense of maturity. Therefore, it was necessary to heat the sulfur-containing compound and the sugar at a high temperature of 70 to ⁇ 180. There was also a restriction of no fat.
  • Patent Document 2 described above was not intended to give a sense of maturity but to obtain a seasoning having a good meat flavor-like flavor. Therefore, it was necessary to heat-react with 5′-nucleotide-containing yeast extract, glutathione-containing yeast extract, monosaccharide, dextrin, and salt.
  • Patent Document 3 Patent Document 4, and Patent Document 5 described above are all intended to obtain a ripening feeling, and are characterized by the use of soy sauce. Therefore, if the taste and flavor derived from soy sauce are desired, but the taste and flavor derived from soy sauce are not necessary for the purpose of aging the food and drink, the balance of the taste and flavor of the food is lost. There was a problem that general-purpose use was difficult.
  • an object of the present invention is to provide a seasoning that can impart a ripening feeling of food, which originally had to be created over a long period of time, in a simple and versatile manner.
  • the inventors of the present invention have completed the present invention as a result of intensive studies to solve the above problems.
  • the present invention includes the following inventions.
  • the oxidation reaction includes a gas supply step of oxygen-containing gas and / or a stirring step, and the oxidation reaction conditions are 2 Ot: 50 or more and 0.5 hours or more 48 A method for producing a seasoning characterized by being less than time.
  • the above-mentioned invention characterized in that the amino acid source and / or peptide source is preheated at 50 to 100 hours, 0.5 hours to 24 hours before mixing with the sugar source. 1) to (4) A method for producing the seasoning according to the above.
  • the present invention is characterized in that the seasoning produced by the method according to any one of (1) to (5) is mixed so as to be from 0.01% to 99% with respect to the food and drink. Food and drink.
  • the present invention includes an arbitrary combination of these components, and an expression in which the expression of the present invention is replaced between a composition and a device.
  • the seasoning which can provide a high-quality and high-quality ripening feeling with respect to food-drinks can be provided.
  • the aging feeling in the present invention relates to the rich taste, which is a concept centered on flavor and taste, and among the rich tastes, those with a solid, broad and thick thickness are defined as having an aging feeling.
  • the unity means that the taste is not legitimate and is in order.
  • the term “spread” refers to a state in which the flavor and taste spread in the oral cavity
  • the term “thickness” in the present invention refers to a state in which a tasty taste is felt as a base of taste. Point to.
  • the raw material of the amino acid source and peptide source in the present invention is not particularly limited as long as it is a nitrogen source that can be used in foods and drinks, and the object can be achieved even with amino acids and peptides.
  • yeast hydrolysates, protein hydrolysates such as fermented seasonings and amino acid solutions, livestock meat extracts, seafood extracts, fish sauces, etc. are preferred from the viewpoint of being inexpensive and readily available in large quantities.
  • the yeast extract includes baker's yeast extract, beer yeast extract, torula yeast extract and the like, and is not particularly limited as long as it is suitable for food.
  • yeast extract extraction methods include, but are not limited to, autolysis, enzymatic decomposition, and hydrothermal decomposition.
  • the protein hydrolyzate is not particularly limited as long as it is suitable for edible use, but a fermented seasoning or an amino acid solution is more preferable because it has a unique flavor and is rich in amino acid sources and peptide sources.
  • the fermented seasoning refers to a protein hydrolyzed by mixing protein such as soybean or wheat and liquid koji, solid koji, or degrading enzyme.
  • a ripening feeling can be imparted even if the nitrogen source is soy sauce, but there is also a taste and flavor peculiar to soy sauce, which is not preferable when it is desired to improve only the aging feeling.
  • soy sauces are manufactured by any of the following methods: main brewing, mixed brewing, and mixing. Is defined in the Japanese Agricultural and Forestry Standard (JAS).
  • JS Japanese Agricultural and Forestry Standard
  • fermented seasonings are particularly mentioned as raw materials for suppressing the taste and taste other than aging.
  • Examples of production method 1 of a preferred fermented seasoning include: (1) A step of inoculating a raw material containing defatted soybeans with microorganisms capable of hydrolyzing protein to produce solid koji, (2) Specific to the obtained solid koji It includes a step of adding mash to make the concentration of sodium chloride, hydrolyzing the protein, and a salt concentration that does not inhibit the hydrolysis of the protein. 1 4 A method for producing a seasoning characterized by allowing moromi to act for 4 hours. When the fermented seasoning prepared by this method is used as an amino acid source and / or peptide source, it is less likely to have a different flavor or taste, and the aging value is particularly well balanced. Is also very good.
  • Examples of preferred fermented seasonings include microbial cultures such as koji molds and yeasts with enhanced glutaminase activity, in the presence of protein degrading enzymes, salt-free or salt concentration of 3% (by volume) or less And a method for producing a seasoning characterized by acting on proteins such as wheat gluten under the above conditions. If the fermented seasoning prepared by this method is used as an amino acid source and a peptide source, it is difficult to produce a different flavor or taste, and the aging feeling is particularly strong and well balanced, which is very preferable.
  • bacteriocin-producing lactic acid bacteria culture solution or its essence is added at the time of koji making Next, the koji obtained was mixed with 1.8 to 3.0 times (by weight) the salt weight of a salt concentration of 13 to 17% by weight of salt solution to form moromi. 3 to 40 to 100, and a method for producing a seasoning characterized by hydrolysis for 11 to 19 days.
  • the fermented seasoning prepared by this method is used as an amino acid source and a Z or peptide source, it is very preferable because it is less likely to have a different flavor or taste, and particularly has a strong aging value and good balance.
  • the amino acid solution is obtained by hydrolyzing various proteins such as soybean and wheat in the presence of an acid such as hydrochloric acid.
  • the sugar source in the present invention is not particularly limited as long as it can be used for food and drink.
  • the sugar source include sucrose, glucose, fructose, xylose, isomerized sugar, etc. Among them, the use of sucrose and glucose is preferable because the ripening feeling becomes stronger.
  • the ratio of the sugar source used is not particularly limited as long as the desired seasoning can be obtained.
  • the sugar source is not less than 0.01 part by weight and not more than 10 parts by weight with respect to 1 part by weight of the amino acid source or peptide source. It can be as follows.
  • the ratio of the sugar source is less than this, it is not preferable because the potency of aging is weak.
  • the ratio of the sugar source is more than this, it is not preferable in that a bad smell (flavor) such as koge odor or a bitter taste is developed.
  • a bad smell such as koge odor or a bitter taste
  • the oxidation reaction it is also important to oxidize the amino acid source and Z or peptide source with the sugar source.
  • oxidation reaction There is no particular limitation on the oxidation reaction.
  • Oxidation reaction can be performed by supplying a gas containing oxygen or stirring.
  • the air supply mentioned here includes a method of directly injecting gas into the mixed liquid using an air hose or the like, and a method of injecting gas into the head space in a sealed container.
  • the amount of air supplied as air per 100 g of seasoning is preferably 10 to 30 ml / min, air supply time is preferably 1 hour to 48 hours, and seasoning 100 g More preferably, the air supply amount is 30 to 120 m / min, and the air supply time is 2 to 12 hours. In this case, it is calculated assuming that 21% oxygen is contained in the air.
  • performing the oxidation reaction by stirring means, for example, stirring vigorously with a homogenizer, a homomixer, a disperser mixer, etc., so as to embed the gas in the seasoning liquid.
  • the stirring power is preferably 0.1 W or more and 20 W or less per kg of seasoning, and more preferably 0.3 W or more and 50 W or less per kg of seasoning. If the air supply and agitation are weak, sufficient sensation cannot be obtained. On the other hand, if it is too strong, an unusual taste such as an egg taste or an unusual flavor is observed, and only a portion of the rich taste becomes stronger and the balance becomes unfavorable.
  • the temperature and time conditions are 20 or more and 50 or less, preferably 0.5 hours or more and 48 or less hours, and 20 X: more than 50 or less and 3 hours or more 2 4 Less than the time is more preferable.
  • the temperature is 20 or less in the oxidation reaction, the aging feeling is weak and the production takes time, resulting in industrially costly problems.
  • the temperature is 6 O: or higher in the oxidation reaction, unpleasant tastes such as burnt odor (flavor), eda, and bitterness are manifested.
  • the preheating conditions are 50 ° C. or more and 1 O O t: or less, preferably 0.1 hours or more and 24 hours or less, more preferably 50 or more and 90 or less, and more preferably 0.5 hours or more and 6 hours or less.
  • the preheating conditions are lower than this and for a short time, the aging feeling is weak and the production takes time, resulting in industrially costly problems.
  • an unpleasant odor such as kogemure odor, an unpleasant taste such as a yag taste, or a bitter taste is unfavorable.
  • the components contributing to the ripening feeling increase by conducting an oxidation reaction or by preheating only the amino acid source and the peptide source.
  • the decoloring step and the deodorizing step include a method of contact treatment with activated carbon, a resin such as ion exchange, alumina, etc., a method of steam distillation or membrane treatment, or any other method.
  • the seasoning obtained in the present invention can be widely used in foods and drinks such as soup, sauce, sauce, soup, fermented food, etc. Especially when used in soup, sauce, etc., it has a rich taste that cannot be imparted unless it is aged for a long time. Is preferable in that it can be reproduced with very high quality.
  • the ratio of the seasoning obtained in the present invention to the food or drink is not particularly limited, but generally it is mixed so that the seasoning is between 0.01% and 99% with respect to the food and drink. Can do.
  • it can be mixed so that the seasoning is 0.05% or more and 20% or less with respect to the food or drink, more preferably 0.1% or more and 5% or less with respect to the food or drink.
  • the ripening feeling imparting agent used for food-drinks can also be obtained.
  • the form of the ripening sensation imparting agent is not particularly limited, but powders, liquids, pastes, and the like are preferable in terms of storage stability and ease of use.
  • Example 1 Difference in the effect of seasonings on noodle soup due to differences in amino acid source and / or peptide source
  • Each amino acid source and / or peptide source and water were adjusted in a 300 ml beaker so that various amino acid sources and / or peptide sources 1 80 m 1 (total nitrogen: T 1 N amount was 2.9 g. Soy sauce used in 1 8 Om 1 is T 1 N amount 2.9 g, so there is no water adjustment), granulated sugar 32 g, mirin 18 g (manufactured by Takara Shuzo Co., Ltd., “Yukara Motomi Rin Junmai”) Were mixed and heated at 80 for 10 minutes to prepare a seasoning liquid.
  • the above-mentioned seasoning liquid which was oxidized using various amino acid sources and peptide or peptide sources as raw materials, was added to mentsuyu (“Nenbentsu Yunomoto” manufactured by Nenben Co., Ltd.) at 1% for sensory evaluation.
  • the sensory evaluation was performed by adding 1% of the above-mentioned seasoning liquid, which was oxidized using various amino acid sources and / or peptide sources, to the noodle soup, but added 1% to the demiglace sauce instead of the noodle soup.
  • the sensory evaluation when added and the sensory evaluation when the seasoning was diluted 5 times with water were also conducted, and it was found that the same tendency was observed.
  • the above-mentioned seasoning liquid which was oxidized by changing the temperature conditions and time, was added to Mentsuyu (“Nenbenyu Yunomoto” manufactured by Nimben Co., Ltd.) at 1% for sensory evaluation.
  • the sensory evaluation results in Table 2 showed that the temperature range of the oxidation reaction was 20 to 50, and the heating time was 3 to 24 hours.
  • 40 is preferably around 4 hours, and it has a high quality with no unusual flavor. A seasoning was obtained.
  • the sensory evaluation was performed by adding 1% of the above-mentioned seasoning liquid, which had been changed in the oxidation reaction conditions, to the noodle soup, but the sensory evaluation when adding 1% to the demiglace sauce instead of the noodle soup A sensory evaluation was also conducted when the seasoning was diluted 5 times with water, and it was found that the same tendency was observed.
  • Example 3 Difference in the effect of addition of seasoning to noodle soup due to differences in the amount of oxygen and time of oxidation reaction
  • the above-mentioned seasoning liquid which was oxidized by changing the air supply conditions and time, was added to noodle soup (“Nimbetsu Yunomoto” manufactured by Ninben Co., Ltd.) for 1%, and sensory evaluation was performed.
  • the air supply amount of the oxidation reaction is effective at 10 to 300 ml Z min per 100 g of seasoning liquid, and from 3 0 to: 1 2 O m 1 min is more preferable It has been shown. It was shown that the oxidation reaction time was effective from 1 hour to 48 hours, and that from 2 hours to 12 hours, the aging feeling became stronger.
  • the sensory evaluation was performed by adding 1% of the above-mentioned seasoning liquid, which had been changed in the oxidation reaction conditions, to the noodle soup, but the sensory evaluation when adding 1% to the demiglace sauce instead of the noodle soup A sensory evaluation was also conducted when the seasoning was diluted 5 times with water, and it was found that the same tendency was observed. .
  • fermented seasoning (“Kojibesu” manufactured by Ajinomoto Co., Inc.) 1 5 5 0 g, granulated sugar 3 0 0 g, water 1 5 0 g
  • the seasoning liquid was prepared by heating for 10 minutes. Then, the seasoning liquid was adjusted to 30, and the homogen spa (TK Ropomix, manufactured by Tokushu Kika Kogyo Co., Ltd.) was used for the seasoning liquid at a temperature of 3 O t: and the stirring speed was 0 to 5 0 0 Stirring was performed at 0 rpm with a stirring time ranging from 1 to 5 hours.
  • the above-mentioned seasoning liquid which was oxidized by changing the rotation conditions and time of stirring, was added to Mentsuyu (“Nenbentsu Yunomoto” manufactured by Nimben Co., Ltd.) at 1% for sensory evaluation.
  • the sensory evaluation was performed by adding 1% of the above-mentioned seasoning liquid, which had been changed in the oxidation reaction conditions, to the noodle soup, but the sensory evaluation when adding 1% to the demiglace sauce instead of the noodle soup The sensory evaluation when the seasoning was diluted 5 times with water was also carried out, and it was found that the same tendency was observed.
  • fermented seasoning (“Koji Base”, Ajinomoto Co., Inc.) 1 80 ml, various sugar sources (sucrose 35 g, glucose 23 g, fructose 20 g and xylose (15 g) were mixed and heated at 80 for 10 minutes to prepare a seasoning liquid.
  • the amount of sugar source added here is different because all sugar sources are adjusted to have an equimolar number.
  • the seasoning liquid with a different sugar source was added to noodle soup ("Nenbentsu Yunomoto" manufactured by Ninben Co., Ltd.) at 1% for sensory evaluation.
  • the sensory evaluation was performed by adding 1% of the above-mentioned seasoning liquid, which had been changed in the oxidation reaction conditions, to the noodle soup, but the sensory evaluation when adding 1% to the demiglace sauce instead of the noodle soup A sensory evaluation was also conducted when the seasoning was diluted 5 times with water, and it was found that the same tendency was observed.
  • fermented seasoning (Koji Base” manufactured by Ajinomoto Co., Inc.) 1 5 5 0 g was preheated.
  • the preheating conditions were a temperature of 50 to 90 ° C. and a time of 0.5 to 6 hours.
  • the pre-heated fermented seasoning was mixed with 30 g of granulated sugar and 1550 g of water, and heated at 80 to 10 minutes to prepare a seasoning liquid.
  • the air was supplied at 30 m 1 / min per 100 g of the seasoning liquid at a temperature of 40 ° C. for 4 hours. 2.
  • the above-mentioned seasoning liquid which had been preheated and then subjected to an oxidation reaction, was added to Mentsuyu (“Nenbentsuyu no Moto” manufactured by Nimbeng Co., Ltd.) at 1% for sensory evaluation.
  • the preheating temperature of the amino acid source and / or peptide source is 7 Ot: ⁇ 8 O t: and the heating time is 1 hour to 2 hours. Indicated.
  • the sensory evaluation is 1% higher than the above-mentioned seasoning liquid with modified oxidation reaction conditions. Sensory evaluation was carried out by adding it, but sensory evaluation when 1% was added to demiglace sauce instead of noodle soup and sensory evaluation when the seasoning was diluted 5 times with water were also carried out. It turned out that the tendency of was seen.
  • Example 6 the seasoning having a preheating condition of 70 t: 2 hours was designated as “Invention product 6 (70 ⁇ 2)”.
  • Commercial soba soup (“Zaru soba soup” manufactured by Kikkoman Co., Ltd.), Demigrass sauce (“Demigrass sauce” manufactured by Heinz Japan Co., Ltd.), Sukiyaki sauce (“Sukiyaki sauce” by Ebara Food Industry Co., Ltd.), Grilled meat sauce ("Golden” Sensory evaluation was performed when the product 6 (70 X 2) of the present invention was mixed to 0.2 g to 3 g and 100 g of sauce, respectively.
  • the product of the present invention when the control is a commercial demiglace sauce 3.0 2.9 2.2 3.0 2.5 It is preferable because it suppresses the soy sauce strength. From the sensory evaluation results in Table 7 to Table 10, the product of the present invention has a good balance of cohesiveness, spread, and thickness in various foods while suppressing undesirable cadence. It has been shown that a preferable aging feeling is imparted by giving.
  • Kado Kado means that the flavor and taste are felt apart, and the unique flavor and taste are strong.
  • the seasoning is mixed with the food and drink so that the concentration is 0.05% to 20%.
  • the seasoning is 0.1% or more and 5% or less for food and drink.
  • the seasoning which can provide a high-quality and high quality aging feeling to food-drinks can be provided.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Seasonings (AREA)

Abstract

Disclosed is a flavoring material for imparting an aged flavor to a food or beverage. The flavoring material can be produced by the oxidation reaction of an amino acid source and/or a peptide source and a sugar source.

Description

明 細 書 熟成感を付与する調味料の製造方法 技術分野  Description Method of producing seasonings that give a sense of maturity Technical Field
本発明は、 飲食品に高力価で良質な熟成感を付与できる調味料の製造法およびそ の調味料の利用に関する。 背景技術  The present invention relates to a method for producing a seasoning capable of imparting a high-titer and high-quality ripening feeling to food and drink and use of the seasoning. Background art
近年、 飲食品に対する本格的な味付けを目的として、 コク味を付与する調味料が 広く利用されている。 本格的という視点から、 短期間で製造した加工食品などでも 熟成感が十分にあるものが望まれている。 一般的なコク味を付与できるものとして は、 たんぱく加水分解物や動物エキスや酵母エキスや、 それらと糖類等との加熱反 応により得られる調味料などが様々な食品に利用されている。  In recent years, seasonings that give a rich taste have been widely used for the purpose of full-fledged seasoning of food and drink. From a full-fledged point of view, processed foods manufactured in a short period of time are desired to have sufficient aging. Proteins that can be given a general rich taste, such as protein hydrolysates, animal extracts and yeast extracts, and seasonings obtained by heat reaction of these with sugars, etc., are used in various foods.
例えば含硫化合物を含む酵母エキスに糖類や必要に応じてアミノ酸を添加し加熱 することを特徴とする、 良好な口一ストミートフレーバー様風味を有する調味料の 技術が知られている (特許文献 1参照) 。  For example, a seasoning technology having a good mouth-to-mouth flavor flavor is known, which is characterized in that a sugar extract or an amino acid as necessary is added to a yeast extract containing a sulfur-containing compound and heated. 1)
また、 5 ' —ヌクレオチド含有酵母エキス、 グル夕チオン酵母エキス、 単糖類、 デキストリン及び食塩を含有した水溶液等を加熱することを特徴とする、 良好なミ 一トフレーバー様風味を有する調味料の技術が知られている (特許文献 2参照) 。  In addition, a seasoning technology having a good flavor-like flavor, characterized by heating an aqueous solution containing 5′-nucleotide-containing yeast extract, glutathione yeast extract, monosaccharide, dextrin and salt, etc. Is known (see Patent Document 2).
熟成感という観点では、 醤油類にみりんや甘味糖類などを加えて製造するかえ しについて、 短期間で熟成感を得る技術開発が進んでいる。  From the standpoint of aging, the development of technology for obtaining a feeling of aging in a short period of time is being promoted for the production of soy sauces by adding mirin and sweet sugar.
例えば、 醤油類に甘味糖類を含有させた後、 空気にさらした状態で壁面上を伝わ らせて流すかえしの製造方法 (特許文献 3参照) 、 醤油にみりんや甘味糖類などを 加えて混和し、 開放型の容器に入れて所定の撹拌所要動力で撹拌するかえしの製造 方法 (特許文献 4参照) 、 醤油類に糖類及び 又はみりんを混和して調製した後に 容器に貯留し、 その後、 容器内に強制的に空気を吹き込むことによって泡沫状の空 気と接触させて熟成を行う製造方法 (特許文献 5参照) 、 などが知られている。 特開平 0 4— 6 6 0 6 9号公報 For example, after adding sweet saccharides to soy sauces, a method of making a maple that flows along the wall surface while exposed to air (see Patent Document 3), add mirin or sweet saccharides to soy sauce, and mix. , A method of manufacturing a maple that is put into an open container and stirred with a predetermined power required for stirring (see Patent Document 4), prepared by mixing sugar and / or mirin with soy sauce, and then stored in the container, and then in the container There is known a production method (see Patent Document 5) in which aging is performed by forcing air into a bubble to bring it into contact with foamy air. Japanese Unexamined Patent Publication No. 0 4-6 6 0 6 9
特許文献 2 Patent Document 2
特開 2 0 0 3— 1 6 9 6 2 7号公報  JP 2 0 0 3-1 6 9 6 2 7
特許文献 3 Patent Document 3
特開 2 0 0 3— 6 1 6 0 8号公報  JP 2 0 0 3-6 1 6 0 8
特許文献 4 Patent Document 4
特開 2 0 0 1— 1 4 5 4 7 0号公報  JP 2 0 0 1-1 4 5 4 7 0
特許文献 5 Patent Document 5
特開 2 0 0 5— 2 8 7 4 3 3号公報 発明の開示  [Patent Document 1] Japanese Patent Laid-Open No. 2 0 0 5-2 8 7 4 3 3 Disclosure of Invention
ところが上述した特許文献 1の発明は、 熟成感の付与ではなく、 良好なロースト ミートフレーバー様風味を有する調味料を得ることが目的であった。 そのため、 含 硫化合物と糖を 7 0で〜 1 8 0でと高温で加熱反応させる必要があった。 また脂肪 非存在下という制限も存在した。  However, the invention of Patent Document 1 described above was aimed at obtaining a seasoning having a good roasted meat flavor-like flavor rather than imparting a sense of maturity. Therefore, it was necessary to heat the sulfur-containing compound and the sugar at a high temperature of 70 to ˜180. There was also a restriction of no fat.
また上述した特許文献 2も、 熟成感の付与ではなく、 良好なミートフレーバー様 風味を有する調味料を得ることが目的であった。 そのため、 5 '—ヌクレオチド含 有酵母エキス、 グル夕チオン含有酵母エキス、 単糖類、 デキストリン、 食塩をすベ て含有させ加熱反応させる必要があった。  Further, Patent Document 2 described above was not intended to give a sense of maturity but to obtain a seasoning having a good meat flavor-like flavor. Therefore, it was necessary to heat-react with 5′-nucleotide-containing yeast extract, glutathione-containing yeast extract, monosaccharide, dextrin, and salt.
また上述した特許文献 3、 特許文献 4、 特許文献 5については、 いずれもかえし の熟成感を得るのが目的であり、 醤油類を使用することに特徴がある。 そのため醤 油由来の味や風味が望まれる場合は良いが、 飲食品の熟成感のみを目的として醤油 由来の味や風味が不要である場合には、 食品の味や風味のバランスが崩れるため、 汎用的な使用は困難であるという課題が存在した。  In addition, Patent Document 3, Patent Document 4, and Patent Document 5 described above are all intended to obtain a ripening feeling, and are characterized by the use of soy sauce. Therefore, if the taste and flavor derived from soy sauce are desired, but the taste and flavor derived from soy sauce are not necessary for the purpose of aging the food and drink, the balance of the taste and flavor of the food is lost. There was a problem that general-purpose use was difficult.
上記背景下において、 本発明は、 本来長時間かけて作成しなければならなかった 食品の熟成感を簡便かつ汎用的に付与できる調味料を提供することを目的とする。 本発明者らは、 上記課題を解決するために鋭意検討を行った結果、 本発明を完成 した。 本発明は以下の各発明を包含する。  In view of the above background, an object of the present invention is to provide a seasoning that can impart a ripening feeling of food, which originally had to be created over a long period of time, in a simple and versatile manner. The inventors of the present invention have completed the present invention as a result of intensive studies to solve the above problems. The present invention includes the following inventions.
( 1 ) 醤油類を除くアミノ酸源および またはべプチド源と糖源を酸化反応させる 工程を含む調味料の製造方法において、 酸化反応が酸素を含む気体の給気工程およ び または撹拌工程を含み、 かつ酸化反応条件が 2 Ot:以上 50で以下、 0. 5時 間以上 48時間以下である事を特徴とする調味料の製造方法。 (1) Oxidation reaction of amino acid source and / or peptide source and sugar source excluding soy sauce In the seasoning manufacturing method including the steps, the oxidation reaction includes a gas supply step of oxygen-containing gas and / or a stirring step, and the oxidation reaction conditions are 2 Ot: 50 or more and 0.5 hours or more 48 A method for producing a seasoning characterized by being less than time.
(2) アミノ酸源および またはペプチド源が、 脱色およびノまたは脱臭工程を経 て製造された酵母エキスまたはたん白加水分解物である上記発明 (1) 記載の調味 料の製造方法。  (2) The method for producing a seasoning according to the above-mentioned invention (1), wherein the amino acid source and / or peptide source is a yeast extract or a protein hydrolyzate produced through a decolorization and no- or deodorization step.
(3)酸素供給条件が、調味料 100 gあたり空気として給気量 10m l /分以上、 300m l Z分以下、 給気時間 1時間以上 48時間以内である上記発明 (1) 記載 の調味料の製造方法。  (3) The seasoning according to the above invention (1), wherein the oxygen supply conditions are air supply amount of 10 ml / min or more, 300 ml / min or less, air supply time of 1 hour or more and 48 hours or less as air per 100 g of seasoning Manufacturing method.
(4) 酸素供給条件が、 空気と接触する状態での撹拌下で、 調味料 l k gあたり撹 拌動力 0. 1 W以上 200W以下である上記発明 (1) 記載の調味料の製造方法 (4) The method for producing a seasoning according to the above invention (1), wherein the oxygen supply condition is stirring power in a state of contact with air and the stirring power is 0.1 W or more and 200 W or less per lkg of seasoning
(5) アミノ酸源および またはペプチド源に対して、 糖源との混合前に、 50で 以上 1 00 以下、 0. 5時間以上 24時間以下の予備加熱を行うことを特徴とす る上記発明 (1) ないし (4) 記載の調味料の製造方法。 (5) The above-mentioned invention characterized in that the amino acid source and / or peptide source is preheated at 50 to 100 hours, 0.5 hours to 24 hours before mixing with the sugar source. 1) to (4) A method for producing the seasoning according to the above.
(6)上記発明(1)ないし (5)のいずれか 1項記載の方法で製造される調味料。 (6) A seasoning produced by the method according to any one of the inventions (1) to (5).
(7) 上記発明 (1) ないし (5) のいずれか 1項記載の方法で製造される調味料 を飲食品に対して 0. 01 %以上 99 %以下となるように混合することを特徴とす る飲食品。 (7) The present invention is characterized in that the seasoning produced by the method according to any one of (1) to (5) is mixed so as to be from 0.01% to 99% with respect to the food and drink. Food and drink.
(8) 上記発明 (1) ないし (5) のいずれか 1項記載の方法で製造される熟成感 付与剤。  (8) A ripening sensation imparting agent produced by the method according to any one of (1) to (5) above.
なお本発明は、 これらの各構成の任意の組み合わせや、 本発明の表現を組成物、 装置などの間で置きかえたものも含む。  Note that the present invention includes an arbitrary combination of these components, and an expression in which the expression of the present invention is replaced between a composition and a device.
本発明によれば、 飲食品に対して高力価で良質な熟成感を付与できる調味料を提 供することが出来る。 発明を実施するための最良の形態  ADVANTAGE OF THE INVENTION According to this invention, the seasoning which can provide a high-quality and high-quality ripening feeling with respect to food-drinks can be provided. BEST MODE FOR CARRYING OUT THE INVENTION
本発明における熟成感とは、 風味、 呈味を中心とした概念であるコク味に関する ものであり、 コク味の中でも、 まとまり、 ひろがり、 厚みが強まったものを熟成感 があると定義した。 ここで本発明においてまとまりとは、 味にカドがなく整ってお り、 まろやかな状態を指し、 本発明においてひろがりとは、 口腔内に風味、 呈味が ひろがる状態を指し、 本発明において厚みとは、 味のベースとしてしつかりとした 味を感じる状態のことを指す。 The aging feeling in the present invention relates to the rich taste, which is a concept centered on flavor and taste, and among the rich tastes, those with a solid, broad and thick thickness are defined as having an aging feeling. Here, in the present invention, the unity means that the taste is not ridiculous and is in order. In the present invention, the term “spread” refers to a state in which the flavor and taste spread in the oral cavity, and the term “thickness” in the present invention refers to a state in which a tasty taste is felt as a base of taste. Point to.
本発明におけるアミノ酸源やペプチド源の原料は、 飲食品に使用できる窒素源で あれば特に限定はなくアミノ酸やペプチドでも目的は達せられる。 しかし、 酵母ェ キス、発酵調味料やアミノ酸液などのたん白加水分解物、畜肉エキス、魚介エキス、 魚醤などが、 工業的な製造を考えると安価で大量に入手しやすい点で好ましい。 ここで酵母エキスとは、 パン酵母エキス、 ビール酵母エキス、 トルラ酵母エキス など挙げられ、 食用に適するものであれば特に制限はない。 酵母エキスの抽出法に 関しても、 自己消化法、 酵素分解法、 熱水分解法などが挙げられるが、 特にこれら に限定されない。  The raw material of the amino acid source and peptide source in the present invention is not particularly limited as long as it is a nitrogen source that can be used in foods and drinks, and the object can be achieved even with amino acids and peptides. However, yeast hydrolysates, protein hydrolysates such as fermented seasonings and amino acid solutions, livestock meat extracts, seafood extracts, fish sauces, etc. are preferred from the viewpoint of being inexpensive and readily available in large quantities. Here, the yeast extract includes baker's yeast extract, beer yeast extract, torula yeast extract and the like, and is not particularly limited as long as it is suitable for food. Examples of yeast extract extraction methods include, but are not limited to, autolysis, enzymatic decomposition, and hydrothermal decomposition.
たん白加水分解物も食用に適するものであれば特に制限はないが、 特有の風味が 弱くアミノ酸源やべプチド源を豊富に含むため発酵調味料やアミノ酸液がより好ま しい。 本発明では発酵調味料とは、 大豆や小麦等のたんぱく質と液体麹、 固体麹や 分解酵素とを混合し、 たん白加水分解させたものを指す。 本発明においては、 窒素 源が醤油類であっても熟成感を付与することは出来るが、 醤油特有の味や風味も存 在するため、 熟成感のみを向上させたいときには好ましくない。  The protein hydrolyzate is not particularly limited as long as it is suitable for edible use, but a fermented seasoning or an amino acid solution is more preferable because it has a unique flavor and is rich in amino acid sources and peptide sources. In the present invention, the fermented seasoning refers to a protein hydrolyzed by mixing protein such as soybean or wheat and liquid koji, solid koji, or degrading enzyme. In the present invention, a ripening feeling can be imparted even if the nitrogen source is soy sauce, but there is also a taste and flavor peculiar to soy sauce, which is not preferable when it is desired to improve only the aging feeling.
ここで醤油類とは、 本醸造、 混合醸造、 混合のいずかの製法で製造されているも のであり、 種類としては濃口醤油、 淡口醤油、 たまり醤油、 再仕込み醤油、 白醤油 の 5種類について、 日本農林規格 (J A S ) において定義されている。  Here, soy sauces are manufactured by any of the following methods: main brewing, mixed brewing, and mixing. Is defined in the Japanese Agricultural and Forestry Standard (JAS).
本発明において、 熟成感以外の異味、 異風味を抑える原料として特に以下の発酵 調味料が挙げられる。  In the present invention, the following fermented seasonings are particularly mentioned as raw materials for suppressing the taste and taste other than aging.
好ましい発酵調味料の製法例 1として、 (1 ) 脱脂大豆を含む原料にたん白質を 加水分解する能力を有する微生物を接種して固体麹を作成する工程、 (2 ) 得られ た固体麹に特定濃度の食塩となるように仕込液を加えて諸味を作成し、 たん白質を 加水分解させる工程を含み、たん白質の加水分解が阻害されない程度の食塩濃度で、 5〜4 5でで 4 0〜 1 4 4時間諸味を作用させることを特徴とする調味料の製造法 が挙げられる。 この製法で作成した発酵調味料をアミノ酸源および/またはべプチ ド源とすると、 異風味も異味の発生もしにくく、 特に熟成感のカ価も強くバランス も良くなり、 非常に好ましい。 Examples of production method 1 of a preferred fermented seasoning include: (1) A step of inoculating a raw material containing defatted soybeans with microorganisms capable of hydrolyzing protein to produce solid koji, (2) Specific to the obtained solid koji It includes a step of adding mash to make the concentration of sodium chloride, hydrolyzing the protein, and a salt concentration that does not inhibit the hydrolysis of the protein. 1 4 A method for producing a seasoning characterized by allowing moromi to act for 4 hours. When the fermented seasoning prepared by this method is used as an amino acid source and / or peptide source, it is less likely to have a different flavor or taste, and the aging value is particularly well balanced. Is also very good.
好ましい発酵調味料の例 2として、 グル夕ミナ一ゼ活性が増強された麹菌、 酵母 などの微生物培養物を、 たん白分解酵素の存在下、 無塩もしくは食塩濃度 3 % (重 量 容量) 以下の条件下で小麦グルテン等のたん白質に作用させることを特徴とす る調味料の製造法が挙げられる。 この製法で作成した発酵調味料をアミノ酸源およ びノまたはペプチド源とすると、 異風味も異味の発生もしにくく、 特に熟成感のカ 価も強くバランスも良くなり、 非常に好ましい。  Examples of preferred fermented seasonings include microbial cultures such as koji molds and yeasts with enhanced glutaminase activity, in the presence of protein degrading enzymes, salt-free or salt concentration of 3% (by volume) or less And a method for producing a seasoning characterized by acting on proteins such as wheat gluten under the above conditions. If the fermented seasoning prepared by this method is used as an amino acid source and a peptide source, it is difficult to produce a different flavor or taste, and the aging feeling is particularly strong and well balanced, which is very preferable.
好ましい発酵調味料の例 3として、 脱脂大豆を主原料に、 麹菌を用いて固体麹を 作成し、 調味液を製造する工程において、 製麹時にバクテリオシン産生乳酸菌培養 液又はその上精を添加して製麹し、 次に得られた麹を麹重量の 1 . 8〜3 . 0倍量 (重量) の食塩濃度 1 3〜 1 7重量%の食塩水と混合し諸味を形成した後に、 諸味 を 3 2〜 4 0で、 1 1〜1 9日間加水分解することを特徴とする調味料の製造法が 挙げられる。 この製法で作成した発酵調味料をアミノ酸源および Zまたはペプチド 源とすると、 異風味も異味の発生もしにくく、 特に熟成感のカ価も強くバランスも 良くなり、 非常に好ましい。 ,  As an example of a preferred fermented seasoning 3, in the process of producing solid seasoning using koji mold using defatted soybean as the main raw material and producing the seasoning liquid, bacteriocin-producing lactic acid bacteria culture solution or its essence is added at the time of koji making Next, the koji obtained was mixed with 1.8 to 3.0 times (by weight) the salt weight of a salt concentration of 13 to 17% by weight of salt solution to form moromi. 3 to 40 to 100, and a method for producing a seasoning characterized by hydrolysis for 11 to 19 days. When the fermented seasoning prepared by this method is used as an amino acid source and a Z or peptide source, it is very preferable because it is less likely to have a different flavor or taste, and particularly has a strong aging value and good balance. ,
アミノ酸液は、 大豆や小麦などの各種たんぱく質を塩酸などの酸存在下でたんぱ く加水分解させたものである。  The amino acid solution is obtained by hydrolyzing various proteins such as soybean and wheat in the presence of an acid such as hydrochloric acid.
本発明における糖源は、 飲食品に使用できるものであれば特に限定はない。 糖源 の例としては、 スクロース、 グルコース、 フルクト一ス、 キシロース、 異性化糖等 が挙げることができ、 その中でもスクロース、 グルコースを使用するとより熟成感 が強まるため好ましい。 糖源の使用割合は、 目的の調味料が得られる限りは特別の 制限はないが、 例えば、 アミノ酸源やペプチド源 1重量部に対して、 糖源 0 . 0 1 重量部以上 1 0重量部以下とすることができる。  The sugar source in the present invention is not particularly limited as long as it can be used for food and drink. Examples of the sugar source include sucrose, glucose, fructose, xylose, isomerized sugar, etc. Among them, the use of sucrose and glucose is preferable because the ripening feeling becomes stronger. The ratio of the sugar source used is not particularly limited as long as the desired seasoning can be obtained. For example, the sugar source is not less than 0.01 part by weight and not more than 10 parts by weight with respect to 1 part by weight of the amino acid source or peptide source. It can be as follows.
糖源の割合がこれ以下だと、 熟成感の力価が弱い点で好ましくない。 If the ratio of the sugar source is less than this, it is not preferable because the potency of aging is weak.
糖源の割合がこれ以上だと、 コゲ臭などの異臭 (風味) や苦味などの異味が発現す る点で好ましくない。 If the ratio of the sugar source is more than this, it is not preferable in that a bad smell (flavor) such as koge odor or a bitter taste is developed.
本発明においては、 アミノ酸源および Zまたはペプチド源と糖源を酸化反応させ ることも重要である。 酸化反応に特に限定はないが、 例えばアミノ酸源やペプチド 源と糖を調理釜などの温度調節可能な装置に投入後、 これら原料に対して空気など 酸素を含む気体を給気する、 もしくは撹拌することにより酸化反応をさせることが 出来る。 ここで言う給気とは、 エアホース等を用いて直接混合液体へ気体を吹き込 む方法や、 密閉された容器内のへッドスペースへ気体を吹き込む方法などが挙げら れる。 気体の給気に関しては、 調味料 1 0 0 gあたり空気として給気量 1 0以上 3 0 0 m l /分、 給気時間 1時間以上 4 8時間以内が好ましく、 調味料 1 0 0 gあた り空気として給気量 3 0以上 1 2 0 m 1 /分、 給気時間 2時間以上 1 2時間以下が より好ましい。 この場合、 空気中に酸素が 2 1 %含まれるとして計算している。 ま た撹拌により酸化反応を行うとは、 例えば、 ホモジナイザー、 ホモミキサ、 デイス パミキサ等により強く撹拌し、 気体を調味液中へ抱き込むように撹拌することを言 う。 攪拌動力に関しては、 調味料 1 k gあたり、 0 . 1 W以上 2 0 0 W以下が好ま しく、 調味料.1 k gあたり、 0 . 3 W以上 5 0 W以下がより好ましい。 給気や攪拌 が弱い場合には、 十分な熟成感が得られない。 また強すぎる場合にはェグ味等の異 味や、 異風味の発現が見られ、 また一部のコク味のみが強まりバランスが悪くなる ため好ましくない。 In the present invention, it is also important to oxidize the amino acid source and Z or peptide source with the sugar source. There is no particular limitation on the oxidation reaction. For example, after the amino acid source, peptide source, and sugar are introduced into a temperature-controllable device such as a cooking pot, air is added to these raw materials. Oxidation reaction can be performed by supplying a gas containing oxygen or stirring. The air supply mentioned here includes a method of directly injecting gas into the mixed liquid using an air hose or the like, and a method of injecting gas into the head space in a sealed container. Regarding gas supply, the amount of air supplied as air per 100 g of seasoning is preferably 10 to 30 ml / min, air supply time is preferably 1 hour to 48 hours, and seasoning 100 g More preferably, the air supply amount is 30 to 120 m / min, and the air supply time is 2 to 12 hours. In this case, it is calculated assuming that 21% oxygen is contained in the air. In addition, performing the oxidation reaction by stirring means, for example, stirring vigorously with a homogenizer, a homomixer, a disperser mixer, etc., so as to embed the gas in the seasoning liquid. The stirring power is preferably 0.1 W or more and 20 W or less per kg of seasoning, and more preferably 0.3 W or more and 50 W or less per kg of seasoning. If the air supply and agitation are weak, sufficient sensation cannot be obtained. On the other hand, if it is too strong, an unusual taste such as an egg taste or an unusual flavor is observed, and only a portion of the rich taste becomes stronger and the balance becomes unfavorable.
本発明における酸化反応において、温度と時間の条件は、 2 0で以上 5 0で以下、 0 . 5時間以上 4 8時間以下が好ましく、 2 0 X:以上 5 0で以下、 3時間以上 2 4 時間以下がより好ましい。 酸化反応において温度が 2 0で以下の場合、 熟成感のカ 価が弱いと共に製造に時間を要し工業的にコストのかかる問題が生じる。 また酸化 反応において温度が 6 O :以上の場合、 コゲ臭 (風味) などやエダ味、 苦味などの 異味が発現するため好ましくない。 またアミノ酸源やペプチド源と糖源で酸化反応 をする前に、 酵母エキス、 たんぱく加水分解物などのアミノ酸源、 ペプチド源のみ を予備加熱することで熟成感がより強まるため好ましい。 予備加熱条件は、 5 0 °C 以上 l O O t:以下、 0 . 1時間以上 2 4時間以下が好ましく、 5 0で以上 9 0で以 下、 0 . 5時間以上 6時間以下がより好ましい。 予備加熱条件がこれより低温や短 時間の場合、 熟成感のカ価が弱いと共に製造に時間を要し工業的にコストのかかる 問題が生じる。 また予備加熱条件がこれより高温や長時間の場合、 コゲゃムレ臭な どの異臭 (風味) ゃェグ味、 苦味などの異味が発現するため好ましくない。  In the oxidation reaction of the present invention, the temperature and time conditions are 20 or more and 50 or less, preferably 0.5 hours or more and 48 or less hours, and 20 X: more than 50 or less and 3 hours or more 2 4 Less than the time is more preferable. When the temperature is 20 or less in the oxidation reaction, the aging feeling is weak and the production takes time, resulting in industrially costly problems. In addition, when the temperature is 6 O: or higher in the oxidation reaction, unpleasant tastes such as burnt odor (flavor), eda, and bitterness are manifested. In addition, it is preferable to preheat only an amino acid source such as yeast extract or protein hydrolyzate or a peptide source before the oxidation reaction with an amino acid source or a peptide source and a sugar source, since the aging feeling becomes stronger. The preheating conditions are 50 ° C. or more and 1 O O t: or less, preferably 0.1 hours or more and 24 hours or less, more preferably 50 or more and 90 or less, and more preferably 0.5 hours or more and 6 hours or less. When the preheating conditions are lower than this and for a short time, the aging feeling is weak and the production takes time, resulting in industrially costly problems. In addition, when the preheating conditions are higher than this or for a long time, an unpleasant odor (flavor) such as kogemure odor, an unpleasant taste such as a yag taste, or a bitter taste is unfavorable.
本発明において熟成感に寄与する成分は、 酸化反応を行うことや、 アミノ酸源や ペプチド源のみを予備加熱しておくことにより、 増加することが見出された。 本発明における、 脱色工程、 脱臭工程とは、 活性炭、 イオン交換などの樹脂、 ァ ルミナなどと接触処理する方法、 水蒸気蒸留や膜処理する方法、 またはその他の任 意の方法が挙げられる。 In the present invention, it has been found that the components contributing to the ripening feeling increase by conducting an oxidation reaction or by preheating only the amino acid source and the peptide source. In the present invention, the decoloring step and the deodorizing step include a method of contact treatment with activated carbon, a resin such as ion exchange, alumina, etc., a method of steam distillation or membrane treatment, or any other method.
本発明で得られる調味料は、 つゆ、 たれ、 ソース、 スープ、 発酵食品などの飲食 品に幅広く用いることができ、 特につゆ、 たれなどに用いると長時間熟成しなけれ ば付与できないコク味 (熟成感) が非常に高品質に再現できる点で好ましい。 本発 明で得られる調味料の飲食品への添加割合に特に限定は無いが、 一般に飲食品に対' して調味料が 0 . 0 1 %以上 9 9 %以下となるように混合することができる。 好ま しくは飲食品に対して調味料が 0 . 0 5 %以上 2 0 %以下となるように混合するこ とができ、 さらに好ましくは飲食品に対して調味料が 0 . 1 %以上 5 %以下となる ように混合することができる。 調味料の割合が低すぎると効果が得られにくくなり 好ましくない。 調味料の割合が高すぎても熟成感の点では問題ないが、 通常はそれ 程添加しなくても十分な効果が得られる。  The seasoning obtained in the present invention can be widely used in foods and drinks such as soup, sauce, sauce, soup, fermented food, etc. Especially when used in soup, sauce, etc., it has a rich taste that cannot be imparted unless it is aged for a long time. Is preferable in that it can be reproduced with very high quality. The ratio of the seasoning obtained in the present invention to the food or drink is not particularly limited, but generally it is mixed so that the seasoning is between 0.01% and 99% with respect to the food and drink. Can do. Preferably, it can be mixed so that the seasoning is 0.05% or more and 20% or less with respect to the food or drink, more preferably 0.1% or more and 5% or less with respect to the food or drink. Can be mixed so that: If the proportion of the seasoning is too low, it is difficult to obtain the effect, which is not preferable. If the proportion of seasoning is too high, there is no problem in terms of aging, but usually sufficient effects can be obtained even if it is not added so much.
また本発明では、 飲食品に使用する熟成感付与剤も得ることが出来る。 ここで熟 成感付与剤の形態に特に限定はないが、 粉末、 液体、 ペースト等が保存性と使いや すさの点で好ましい。  Moreover, in this invention, the ripening feeling imparting agent used for food-drinks can also be obtained. Here, the form of the ripening sensation imparting agent is not particularly limited, but powders, liquids, pastes, and the like are preferable in terms of storage stability and ease of use.
(実施例)  (Example)
以下、 本発明について実施例でさらに説明するが、 本発明の技術範囲はこれら実 施例によって制限されるものではない。  Hereinafter, the present invention will be further described with reference to examples. However, the technical scope of the present invention is not limited by these examples.
(実施例 1 :アミノ酸源および またはペプチド源の違いによる調味料のめんつゆ への添加効果の違い)  (Example 1: Difference in the effect of seasonings on noodle soup due to differences in amino acid source and / or peptide source)
1 . アミノ酸源および またはべプチド源の選定  1. Selection of amino acid source and / or peptide source
アミノ酸源および/またはペプチド源として、 以下を用いた。  The following were used as amino acid sources and / or peptide sources.
'醤油 (濃口醤油、 ヒゲタ醤油株式会社製、 全窒素: T一 N = l . 6 % )  'Soy Sauce (Dark Mouth Soy Sauce, Higeta Soy Sauce Co., Ltd., Total Nitrogen: T 1 N = l. 6%)
·アミノ酸液 (淡口 「味液」 、 味の素株式会社製)  · Amino acid solution (Tanguchi “Taste Solution” manufactured by Ajinomoto Co., Inc.)
•酵母エキス (酵母エキス N O . 1、 味の素株式会社製)  • Yeast extract (yeast extract N O .1, manufactured by Ajinomoto Co., Inc.)
•発酵調味料 ( 「コウジベース」 、 味の素株式会社製)  • Fermented seasoning (“Koji Base”, manufactured by Ajinomoto Co., Inc.)
•脱色脱臭発酵調味料 ( 「コウジベース」 に対して 1 0 0重量部に対して、 活性炭 ( 「ホクエツ B A」 、 味の素ファインテクノ株式会社製) を 2重量部添加し、 2 4 、 24時間攪拌後、 活性炭をろ過除去したもの。 吸光度 545 nmの値で、 元の 発酵調味料が 0. 7であったのに対して、 本脱色脱臭発酵調味料は 0. 2まで低下 していた。 ) • Decolorizing and deodorizing fermented seasoning (100 parts by weight for “Koji Base” and 2 parts by weight of activated carbon (“Hokuetsu BA” manufactured by Ajinomoto Fine Techno Co., Ltd.) After stirring for 24 hours, activated carbon is filtered off. The value of absorbance at 545 nm was 0.7 for the original fermented seasoning, whereas this decolored deodorized fermented seasoning had decreased to 0.2. )
2. 酸化反応させた調味液の製造  2. Manufacture of seasoning liquid that has undergone oxidation reaction
30 0m l ビーカー内で、 各種アミノ酸源および またはペプチド源 1 8 0m 1 (全窒素: T一 N量が 2. 9 gとなるように各アミノ酸源および またはべプチド 源と水を調整。 実施例で用いた醤油 1 8 Om 1は T一 N量 2. 9 gなので水の調整 無し) 、 グラニュー糖 32 g、 みりん 1 8 g (宝酒造社株式会社製、 「夕カラ本み りん純米」 ) を混合し、 80でで 1 0分間加熱し調味液を作成した。 その後本調味 液を 3 に調整し、 本調味液に対してビーカ一容器底面からエアコンプレッサか らシリコンチューブ (外径 6mm、 内径 4mm) 、 セラミックエアストーン (外径 1 7mmX長 2 Omm) を用いて本調味液 1 00 g当たり 3 Om 1 Z分の空気供給 を 30でで 24時間実施した。  Each amino acid source and / or peptide source and water were adjusted in a 300 ml beaker so that various amino acid sources and / or peptide sources 1 80 m 1 (total nitrogen: T 1 N amount was 2.9 g. Soy sauce used in 1 8 Om 1 is T 1 N amount 2.9 g, so there is no water adjustment), granulated sugar 32 g, mirin 18 g (manufactured by Takara Shuzo Co., Ltd., “Yukara Motomi Rin Junmai”) Were mixed and heated at 80 for 10 minutes to prepare a seasoning liquid. Then, adjust the seasoning liquid to 3 and use a silicon tube (outer diameter 6 mm, inner diameter 4 mm) and ceramic air stone (outer diameter 17 mm x length 2 Omm) from the bottom of the beaker container. Then, air supply of 3 Om 1 Z per 100 g of the seasoning liquid was performed at 30 for 24 hours.
3. 酸化反応させた調味液の官能評価  3. Sensory evaluation of the seasoning liquid after oxidation
各種アミノ酸源およびノまたはペプチド源を原料とし酸化反応させた前述の調味 液をめんつゆ ( 「にんべんつゆの素」 株式会社にんべん社製) に 1 %上乗せ添加し 官能評価を行った。 官能評価は、 コク味 (まとまり、 ひろがり、 厚み) 、 熟成感に ついて、 調味液を添加しないめんつゆをコントロール (0点) として、 0 :無い、 1 :やや強い、 2 :強い、 3 :非常に強い、 として、 n= 1 0で行った。  The above-mentioned seasoning liquid, which was oxidized using various amino acid sources and peptide or peptide sources as raw materials, was added to mentsuyu (“Nenbentsu Yunomoto” manufactured by Nenben Co., Ltd.) at 1% for sensory evaluation. The sensory evaluation is as follows: Mokumi (solid, spread, thickness), aging, and noodle soup without seasoning as a control (0 points): 0: None, 1: Slightly strong, 2: Strong, 3: Very Strong, as n = 1 0.
また、 醤油 (濃口醤油、 ヒゲ夕醤油株式会社製) 1 8 Om lに対し、 グラニュー 糖 32 g、みりん 1 8 gを混合し、 8 0°Cで 1 0分間加熱し調味液を作成したあと、 かえしとして通気性のあるぺットボトルに 24T:で保管した。 0日保管(保管直後)、 1 4日保管したものについても、 一般的なかえしの熟成感付与効果を見る為に、 参 考として評価した。 結果を表 1に示す。 表 1 Also, after making soy sauce (Daiguchi Soy Sauce, Higeyu Soy Sauce Co., Ltd.) 1 8 Oml, granulated sugar 32 g and mirin 18 g are mixed and heated at 80 ° C for 10 minutes to create a seasoning liquid Stored in a breathable pet bottle for 24T: The samples stored for 0 days (immediately after storage) and for 14 days were also evaluated as a reference in order to see the general ripening effect of the maple. The results are shown in Table 1. table 1
アミノ酸源および またはペプチド源の違いによる調味料のめんつゆへの添加 効果の違い  Addition of seasoning to noodle soup due to differences in amino acid source and / or peptide source
Figure imgf000010_0001
表 1の官能評価結果から、 特にアミノ酸液、 酵母エキス、 発酵調味料を用いた場 合に、 よりコク味力価が強く、 異風味ゃ異味の発現も抑えられ、 必要な熟成感が得 られることが示された。 更に活性炭処理を行った脱色脱臭発酵調味料を使用した系 については、 コク味力価が強く熟成感も強くなることが示された。
Figure imgf000010_0001
From the sensory evaluation results in Table 1, especially when amino acid solutions, yeast extracts, and fermented seasonings are used, the kokumi titer is stronger, and the expression of off-flavors can be suppressed if the taste is different, resulting in the necessary aging feeling. It was shown that. Furthermore, the system using the decolorized deodorized fermented seasoning that had been treated with activated carbon was shown to have a strong kokumi titer and a strong aging feeling.
また上記官能評価は、 各種アミノ酸源および またはべプチド源を原料とし酸化 反応させた前述の調味液をめんつゆに 1 %上乗せ添加し官能評価を行ったが、 めん つゆのかわりにデミグラスソースに 1 %上乗せ添加した際の官能評価や、 調味料を 水で 5倍希釈した際の官能評価についても実施を行い、 同様の傾向が見られること が判明した。  In addition, the sensory evaluation was performed by adding 1% of the above-mentioned seasoning liquid, which was oxidized using various amino acid sources and / or peptide sources, to the noodle soup, but added 1% to the demiglace sauce instead of the noodle soup. The sensory evaluation when added and the sensory evaluation when the seasoning was diluted 5 times with water were also conducted, and it was found that the same tendency was observed.
(実施例 2 :温度と時間の酸化反応条件の違いによる調味料のめんつゆへの添加効 果の違い)  (Example 2: Difference in the effect of seasoning on the noodle soup depending on the temperature and time oxidation reaction conditions)
1 . 温度と時間の酸化反応条件の異なる調味液の製造 3 0 0 0 m l ビーカー内で、 発酵調味料 ( 「コウジベース」 、 味の素株式会社製) 1 5 5 0 g、 グラニュー糖 3 0 0 g、 水 1 5 0 gを混合し、 8 で 1 0分間加熱 し調味液を作成した。 その後本調味液を 3 0でに調整し、 本調味液に対してビーカ —容器底面からエアコンプレッサからシリコンチューブ(外径 6 mm、内径 4 mm)、 セラミックエアストーン (外径 1 7 mm X長 2 0 mm) を用いて本調味液 1 0 0 g 当たり 3 0 m 1 /分の空気供給を、 温度条件が 2 0〜 7 0で、 時間条件が 1 ~ 2 4 時間の範囲で実施した。 1. Manufacture of seasonings with different temperature and time oxidation conditions In a 300 ml beaker, mix fermented seasoning ("Koji Base", Ajinomoto Co., Inc.) 1 5 5 0 g, granulated sugar 3 0 0 g, water 1 5 0 g, 8 to 10 minutes A seasoning liquid was prepared by heating. After that, adjust the seasoning liquid to 30 and against the seasoning liquid from the beaker — from the bottom of the container, from the air compressor to the silicon tube (outer diameter 6 mm, inner diameter 4 mm), ceramic air stone (outer diameter 17 mm x long) 20 mm) was used to supply air at a rate of 30 m 1 / min per 100 g of the seasoning liquid at a temperature condition of 20 to 70 and a time condition of 1 to 24 hours.
2 . 酸化反応させた調味液の官能評価 2. Sensory evaluation of the seasoned liquid after oxidation
温度条件と時間を変更させて酸化反応させた前述 調味液をめんつゆ ( 「にんべ んつゆの素」 株式会社にんべん社製) に 1 %上乗せ添加し官能評価を行った。 官能 評価は、 コク味 (まとまり、 ひろがり、 厚み) 、 熟成感について、 調味液を添加し ないめんつゆをコントロール (0点) として、 0 :無い、 1 :やや強い、 2 :強い、 3 :非常に強い、 として、 n = 1 0で行った。  The above-mentioned seasoning liquid, which was oxidized by changing the temperature conditions and time, was added to Mentsuyu (“Nenbenyu Yunomoto” manufactured by Nimben Co., Ltd.) at 1% for sensory evaluation. The sensory evaluation is as follows: for the richness (coarseness, spread, thickness), aging, and the control of the noodle soup with no seasoning liquid added (0 points), 0: none, 1: slightly strong, 2: strong, 3: very Strong, performed as n = 1 0.
結果を表 2に示す。 The results are shown in Table 2.
表 2 Table 2
温度と時間の酸化反応条件の違いによる調味料のめんつゆへの添加効果の違い  Differences in the effect of seasoning on the noodle soup due to differences in temperature and time oxidation conditions
Figure imgf000011_0001
表 2の官能評価結果から、 酸化反応の温度帯は 2 0で〜 5 0 で、 加熱時間は 3 時間〜 2 4時間で、 異風味がせず、 熟成感が強まることが示された。 短時間で力価 の高い熟成感を得るには 4 0でで 4時間前後が好ましく、 異風味もしない高品質な 調味料が得られた。
Figure imgf000011_0001
The sensory evaluation results in Table 2 showed that the temperature range of the oxidation reaction was 20 to 50, and the heating time was 3 to 24 hours. In order to obtain a matured sensation with a high titer in a short time, 40 is preferably around 4 hours, and it has a high quality with no unusual flavor. A seasoning was obtained.
また上記官能評価は、 酸化反応条件を変更した前述の調味液をめんつゆに 1 %上 乗せ添加し官能評価を行ったが、 めんつゆのかわりにデミグラスソースに 1 %上乗 せ添加した際の官能評価や、 調味料を水で 5倍希釈した際の官能評価についても実 施を行い、 同様の傾向が見られることが判明した。  In addition, the sensory evaluation was performed by adding 1% of the above-mentioned seasoning liquid, which had been changed in the oxidation reaction conditions, to the noodle soup, but the sensory evaluation when adding 1% to the demiglace sauce instead of the noodle soup A sensory evaluation was also conducted when the seasoning was diluted 5 times with water, and it was found that the same tendency was observed.
(実施例 3 :酸素量と時間の酸化反応条件の違いによる調味料のめんつゆへの添加 効果の違い)  (Example 3: Difference in the effect of addition of seasoning to noodle soup due to differences in the amount of oxygen and time of oxidation reaction)
1. 酸素量と時間の酸化反応条件の異なる調味液の製造  1. Production of seasoning liquids with different oxygen content and time oxidation reaction conditions
3000m lビーカー内で、 発酵調味料 ( 「コウジベース」 、 味の素株式会社製) 1 550 g、 グラニュー糖 300 g、 水 150 gを混合し、 80でで 10分間加熱 し調味液を作成した。 その後本調味液を 3 Ot:に調整し、 本調味液に対してビーカ 一容器底面からエアコンプレッサからシリコンチューブ(外径 6 mm、内径 4 mm)、 セラミックエアストーン (外径 17mmX長 20mm) を用いて温度 30 t:で本調 味液 1 00 g当たりの空気供給量が 0〜 400 m 1 /分、 時間条件が 1〜 1 2時間 の範囲で実施した。  In a 3000 ml beaker, 1550 g of fermented seasoning (“Koji Base” manufactured by Ajinomoto Co., Inc.), 300 g of granulated sugar and 150 g of water were mixed and heated at 80 for 10 minutes to prepare a seasoning liquid. After that, adjust the seasoning liquid to 3 Ot: From the bottom of the beaker container, add a silicon tube (outer diameter 6 mm, inner diameter 4 mm) and ceramic air stone (outer diameter 17 mm x length 20 mm) to the seasoning liquid. It was carried out at a temperature of 30 t: in an amount of 0 to 400 m 1 / min per 100 g of the seasoning liquid and a time of 1 to 12 hours.
2. 酸化反応させた調味液の官能評価  2. Sensory evaluation of the seasoning liquid after oxidation reaction
空気供給条件と時間を変更させて酸化反応させた前述の調味液をめんつゆ ( 「に んべんつゆの素」 株式会社にんべん社製) に 1 %上乗せ添加し官能評価を行った。 官能評価は、 コク味 (まとまり、 ひろがり、 厚み) 、 熟成感について、 調味液を添 加しないめんつゆをコントロール (0点) として、 0 :無い、 1 :やや強い、 2 : 強い、 3 :非常に強い、 として、 n= 10で行った。  The above-mentioned seasoning liquid, which was oxidized by changing the air supply conditions and time, was added to noodle soup (“Nimbetsu Yunomoto” manufactured by Ninben Co., Ltd.) for 1%, and sensory evaluation was performed. The sensory evaluation is as follows: for the rich taste (unified, spread, thickness), aging, and noodle soup with no seasoning added as a control (0 points), 0: none, 1: slightly strong, 2: strong, 3: very As strong, we went with n = 10.
結果を表 3に示す。 The results are shown in Table 3.
表 3 Table 3
空気供給量と時間の酸化反応条件の違いによる調味料のめんつゆへの添加効果 の違い  Difference in the effect of seasoning on the noodle soup due to the difference in the amount of air supply and time of the oxidation reaction
Figure imgf000013_0001
表 3の官能評価結果から、 酸化反応の空気供給量は調味液 1 0 0 gあたり 1 0 ~ 3 0 0 m l Z分で効果があり、 3 0〜: 1 2 O m 1 分がより好ましいことが示され た。 酸化反応の時間は 1時間〜 4 8時間で効果があり、 2時間〜 1 2時間で、 熟成 感がより強まることが示された。
Figure imgf000013_0001
From the sensory evaluation results in Table 3, the air supply amount of the oxidation reaction is effective at 10 to 300 ml Z min per 100 g of seasoning liquid, and from 3 0 to: 1 2 O m 1 min is more preferable It has been shown. It was shown that the oxidation reaction time was effective from 1 hour to 48 hours, and that from 2 hours to 12 hours, the aging feeling became stronger.
また上記官能評価は、 酸化反応条件を変更した前述の調味液をめんつゆに 1 %上 乗せ添加し官能評価を行ったが、 めんつゆのかわりにデミグラスソースに 1 %上乗 せ添加した際の官能評価や、 調味料を水で 5倍希釈した際の官能評価についても実 施を行い、 同様の傾向が見られることが判明した。 .  In addition, the sensory evaluation was performed by adding 1% of the above-mentioned seasoning liquid, which had been changed in the oxidation reaction conditions, to the noodle soup, but the sensory evaluation when adding 1% to the demiglace sauce instead of the noodle soup A sensory evaluation was also conducted when the seasoning was diluted 5 times with water, and it was found that the same tendency was observed. .
(実施例 4 :撹拌による酸化反応条件の違いによる調味料のめんつゆへの添加効果 の違い) 1 . 撹拌により酸化反応条件の異なる調味液の製造 (Example 4: Difference in the effect of seasoning on the noodle soup due to differences in the oxidation reaction conditions due to stirring) 1. Production of seasoning liquid with different oxidation reaction conditions by stirring
3 0 0 0 m l ビーカ一内で、 発酵調味料 ( 「コウジべ一ス」 、 味の素株式会社製) 1 5 5 0 g、 グラニュー糖 3 0 0 g、 水 1 5 0 gを混合し、 8 0でで 1 0分間加熱 し調味液を作成した。 その後本調味液を 3 0でに調整し、 本調味液に対してホモデ イスパ (T Kロポミクス、 特殊機化工業社製) を用いて温度 3 O t:で、 撹拌回転数 が 0〜 5 0 0 0 r p mで、 撹拌時間が 1〜 5時間までの範囲で撹拌した。  3 0 0 0 ml In a beaker, fermented seasoning (“Kojibesu” manufactured by Ajinomoto Co., Inc.) 1 5 5 0 g, granulated sugar 3 0 0 g, water 1 5 0 g The seasoning liquid was prepared by heating for 10 minutes. Then, the seasoning liquid was adjusted to 30, and the homogen spa (TK Ropomix, manufactured by Tokushu Kika Kogyo Co., Ltd.) was used for the seasoning liquid at a temperature of 3 O t: and the stirring speed was 0 to 5 0 0 Stirring was performed at 0 rpm with a stirring time ranging from 1 to 5 hours.
2 . 酸化反応させた調味液の官能評価  2. Sensory evaluation of the seasoned liquid after oxidation
撹拌の回転条件と時間を変更させて酸化反応させた前述の調味液をめんつゆ(「に んべんつゆの素」 株式会社にんべん社製) に 1 %上乗せ添加し官能評価を行った。 官能評価は、 コク味 (まとまり、 ひろがり、 厚み) 、 熟成感について、 調味液を添 加しないめんつゆをコントロール (0点) として、 0 :無い、 1 :やや強い、 2 : 強い、 3 :非常に強い、 として、 n = 1 0で行った。  The above-mentioned seasoning liquid, which was oxidized by changing the rotation conditions and time of stirring, was added to Mentsuyu (“Nenbentsu Yunomoto” manufactured by Nimben Co., Ltd.) at 1% for sensory evaluation. The sensory evaluation is as follows: for the body taste (coarseness, spread, thickness), aging, and the control of noodle soup without adding seasoning liquid (0 points), 0: none, 1: slightly strong, 2: strong, 3: very Strong, performed as n = 1 0.
結果を表 4に示す。 表 4  The results are shown in Table 4. Table 4
撹拌による酸化反応条件の違いによる調味料のめんつゆへの添加効果の違い  Difference in the effect of seasoning on the noodle soup due to differences in the oxidation reaction conditions due to stirring
Figure imgf000014_0001
3500, 3. 0 2. 2 3. 0 2. 7 56 x 3hour
Figure imgf000014_0001
3500, 3. 0 2. 2 3. 0 2. 7 56 x 3hour
5000rpm 2. 3 1. 5 2. 5 2. 2 ェグ味あり 56 x 3hour 表 4の官能評価結果から、 空気の供給法としてホモミキサ、 ディスパミキサなど の攪拌装置を使用することができることが確認された。 攪拌の回転数は 1 5 0 0〜 3 5 0 0 r p mで、 攪拌時間は 1時間〜 5時間で熟成感が強まることが示された。 さらに攪拌時間は 3時間〜 5時間で熟成感がより強まることが示された。 また回転 数が高すぎる場合や長時間撹拌した場合は、 エダ味などの異味の発生が見られ好ま しくない結果が見られた。  5000rpm 2. 3 1. 5 2. 5 2. 2 Eg taste 56 x 3hour The sensory evaluation results in Table 4 confirm that a stirring device such as a homomixer or disperser can be used as the air supply method. It was. It was shown that the maturation feeling was enhanced when the number of rotations of stirring was 1500 to 3500 rpm and the stirring time was 1 to 5 hours. Furthermore, it was shown that the aging feeling became stronger when the stirring time was 3 hours to 5 hours. In addition, when the number of rotations was too high, or when stirring for a long time, unpleasant results such as eda taste were observed, which was not preferable.
また上記官能評価は、 酸化反応条件を変更した前述の調味液をめんつゆに 1 %上 乗せ添加し官能評価を行ったが、 めんつゆのかわりにデミグラスソースに 1 %上乗 せ添加した際の官能評価や、 調味料を水で 5倍希釈した際の官能評価についても.実 施を行い、 同様の傾向が見られることが判明した。  In addition, the sensory evaluation was performed by adding 1% of the above-mentioned seasoning liquid, which had been changed in the oxidation reaction conditions, to the noodle soup, but the sensory evaluation when adding 1% to the demiglace sauce instead of the noodle soup The sensory evaluation when the seasoning was diluted 5 times with water was also carried out, and it was found that the same tendency was observed.
(実施例 5 :糖源の違いによる調味料のめんつゆへの添加効果の違い)  (Example 5: Difference in the effect of seasoning on the noodle soup depending on the sugar source)
1 . 糖源の異なる調味液の製造  1. Production of seasonings with different sugar sources
3 0 0 m l ビーカ一内で、 発酵調味料 ( 「コウジベース」 、 味の素株式会社製) 1 8 0 m lに対し、 各種糖源 (スクロース 3 5 g、 グルコース 2 3 g、 フルク卜一 ス 2 0 g、 キシロース 1 5 g ) を混合し、 8 0でで 1 0分間加熱し調味液を作成し た。 ここで糖源の添加量が異なるのは、 糖源が全てほぼ等モル数になるように調整 しているためである。 その後本調味液を 3 に調整し、 本調味液に対してビーカ 一容器底面からエアコンプレッサからシリコンチューブ(外径 6 mm、内径 4 mm)、 セラミックエアストーン (外径 1 7 mm X長 2 O mm) を用いて本調味液 1 0 0 g 当たり 3 O m 1 分の空気供給を、 温度 3 0でで、 1 2時間実施した。  In a 300 ml beaker, fermented seasoning (“Koji Base”, Ajinomoto Co., Inc.) 1 80 ml, various sugar sources (sucrose 35 g, glucose 23 g, fructose 20 g and xylose (15 g) were mixed and heated at 80 for 10 minutes to prepare a seasoning liquid. The amount of sugar source added here is different because all sugar sources are adjusted to have an equimolar number. Then adjust the seasoning liquid to 3, and from the bottom of the beaker container to the seasoning liquid, from the air compressor to the silicon tube (outer diameter 6 mm, inner diameter 4 mm), ceramic air stone (outer diameter 17 mm x length 2 O mm) was used, and an air supply of 3 O m 1 minute per 100 g of the seasoning liquid was performed at a temperature of 30 for 12 hours.
2 . 調味液の官能評価  2. Sensory evaluation of seasoning liquid
糖源を変更した調味液をめんつゆ (「にんべんつゆの素」株式会社にんべん社製) に 1 %上乗せ添加し官能評価を行った。官能評価は、 コク味(まとまり、ひろがり、 厚み) 、 熟成感について、 調味液を添加しないめんつゆをコントロール (0点) と して、 0 :無い、 1 :やや強い、 2 :強い、 3 :非常に強い、 として、 n = 1 0で 行った。 The seasoning liquid with a different sugar source was added to noodle soup ("Nenbentsu Yunomoto" manufactured by Ninben Co., Ltd.) at 1% for sensory evaluation. The sensory evaluation consists of controlling the noodle soup without adding seasoning liquid (0 points) for the richness (coarseness, spread, thickness) and aging. 0: None, 1: Slightly strong, 2: Strong, 3: Very strong, n = 10
結果を表 5に示す。  The results are shown in Table 5.
表 5  Table 5
糖源の違いによる調味料のめんつゆへの添加効果の違い  Differences in the effects of seasonings on noodle soup due to differences in sugar sources
Figure imgf000016_0001
表 5の官能評価結果から、 糖源としては、 スクロースやグルコースを使用した場 合に、 異風味、 異味がなく、 より好ましい熟成感が強まることが示された。
Figure imgf000016_0001
From the sensory evaluation results in Table 5, it was shown that when sucrose or glucose is used as the sugar source, there is no off-flavor or off-flavor, and a more preferable aging feeling is enhanced.
また上記官能評価は、 酸化反応条件を変更した前述の調味液をめんつゆに 1 %上 乗せ添加し官能評価を行ったが、 めんつゆのかわりにデミグラスソースに 1 %上乗 せ添加した際の官能評価や、 調味料を水で 5倍希釈した際の官能評価についても実 施を行い、 同様の傾向が見られることが判明した。  In addition, the sensory evaluation was performed by adding 1% of the above-mentioned seasoning liquid, which had been changed in the oxidation reaction conditions, to the noodle soup, but the sensory evaluation when adding 1% to the demiglace sauce instead of the noodle soup A sensory evaluation was also conducted when the seasoning was diluted 5 times with water, and it was found that the same tendency was observed.
(実施例 6 :予備加熱条件の違いによる調味料のめんつゆへの添加効果の違い) 1 . 予備加熱条件の異なる調味液の製造  (Example 6: Difference in the effect of adding seasonings to noodle soup due to differences in preheating conditions) 1. Production of seasonings with different preheating conditions
3 0 0 0 m lビーカー内で、 発酵調味料 ( 「コウジベース」 、 味の素株式会社製) 1 5 5 0 gを予備加熱した。 予備加熱条件は、 温度が 5 0〜 9 0 °C、 時間が 0 . 5 〜 6時間の範囲で実施した。 その後予備加熱した発酵調味料と、 グラニュー糖 3 0 0 g、 水 1 5 0 gを混合し、 8 0でで 1 0分間加熱し調味液を作成した。 その後本 調味液を 4 0でに調整し、 本調味液に対してビーカ一容器底面からエアコンプレ:ソ サからシリコンチューブ(外径 6 mm、 内径 4 mm) 、 セラミックエアストーン(外 径 1 7 mm X長 2 0 mm) を用いて本調味液 1 0 0 g当たり 3 0 m 1 /分の空気供 給を、 温度 4 0 °C、 4時間の条件で実施した。 2 . 調味液の官能評価 In a 300 ml beaker, fermented seasoning ("Koji Base" manufactured by Ajinomoto Co., Inc.) 1 5 5 0 g was preheated. The preheating conditions were a temperature of 50 to 90 ° C. and a time of 0.5 to 6 hours. Thereafter, the pre-heated fermented seasoning was mixed with 30 g of granulated sugar and 1550 g of water, and heated at 80 to 10 minutes to prepare a seasoning liquid. Then adjust the seasoning liquid to 40, and adjust the air conditioning pressure from the bottom of the beaker container to the seasoning liquid: from the soda to the silicon tube (outer diameter 6 mm, inner diameter 4 mm), ceramic air stone (outer diameter 17 mm). The air was supplied at 30 m 1 / min per 100 g of the seasoning liquid at a temperature of 40 ° C. for 4 hours. 2. Sensory evaluation of seasoning liquid
予備加熱を行ってから酸化反応させた前述の調味液をめんつゆ ( 「にんべんつゆ の素」株式会社にんべん社製)に 1 %上乗せ添加し官能評価を行った。官能評価は、 コク味 (まとまり、 ひろがり、 厚み) 、 熟成感について、 調味液を添加しないめん つゆをコントロール (0点) として、 0 :無い、 1 :やや強い、 2 :強い、 3 :非 常に強い、 として、 n = 1 0で行った。  The above-mentioned seasoning liquid, which had been preheated and then subjected to an oxidation reaction, was added to Mentsuyu (“Nenbentsuyu no Moto” manufactured by Nimbeng Co., Ltd.) at 1% for sensory evaluation. The sensory evaluation is as follows: for the richness (coarseness, spread, thickness), aging, and noodle soup without seasoning as a control (0 points): 0: None, 1: Slightly strong, 2: Strong, 3: Very Strong, performed as n = 1 0.
結果を表 6に示す。  The results are shown in Table 6.
表 6  Table 6
予備加熱条件の違いによる調味料のめんつゆへの添加効果の違い  Difference in the effect of seasoning on noodle soup due to differences in preheating conditions
Figure imgf000017_0001
表 6の官能評価結果から、 アミノ酸源および/またはペプチド源の予備加熱の温 度帯は 7 O t:〜 8 O t:で、 加熱時間は 1時間〜 2時間で熟成感が最も強まることが 示された。
Figure imgf000017_0001
From the sensory evaluation results in Table 6, the preheating temperature of the amino acid source and / or peptide source is 7 Ot: ~ 8 O t: and the heating time is 1 hour to 2 hours. Indicated.
また上記官能評価は、 酸化反応条件を変更した前述の調味液をめんつゆに 1 %上 乗せ添加し官能評価を行ったが、 めんつゆのかわりにデミグラスソースに 1 %上乗 せ添加した際の官能評価や、 調味料を水で 5倍希釈した際の官能評価についても実 施を行い、 同様の傾向が見られることが判明した。 In addition, the sensory evaluation is 1% higher than the above-mentioned seasoning liquid with modified oxidation reaction conditions. Sensory evaluation was carried out by adding it, but sensory evaluation when 1% was added to demiglace sauce instead of noodle soup and sensory evaluation when the seasoning was diluted 5 times with water were also carried out. It turned out that the tendency of was seen.
(実施例 7 :本願発明の調味料の飲食品への添加効果)  (Example 7: Effect of adding seasoning of the present invention to food and drink)
実施例 6において予備加熱条件が 70t:、 2時間である調味料を「本発明品 6 (7 0 X 2) 」 とした。市販のそばつゆ ( 「ざるそばつゆ」 キッコ一マン株式会社製) 、 デミグラスソース ( 「デミグラスソース」 ハインツ日本株式会社製) 、 すき焼きの たれ ( 「すき焼きのたれ」 ェバラ食品工業社製) 、 焼き肉のたれ ( 「黄金のたれ中 辛」 ェバラ食品工業社製) に、 本発明品 6 (70 X 2) をそれぞれ 0. 2 g~3 g たれ 100 gとなるように混合したときの官能評価を行った。 官能評価は、 コク 味 (まとまり、 ひろがり、 厚み) 、 熟成感について、 調味液を添加しない各種市販 品をコントロール (0点) として、 0 :無い、 1 :やや強い、 2 :強い、 3 :非常 に強い、 として、 n= 1 0で行った。  In Example 6, the seasoning having a preheating condition of 70 t: 2 hours was designated as “Invention product 6 (70 × 2)”. Commercial soba soup ("Zaru soba soup" manufactured by Kikkoman Co., Ltd.), Demigrass sauce ("Demigrass sauce" manufactured by Heinz Japan Co., Ltd.), Sukiyaki sauce ("Sukiyaki sauce" by Ebara Food Industry Co., Ltd.), Grilled meat sauce ("Golden" Sensory evaluation was performed when the product 6 (70 X 2) of the present invention was mixed to 0.2 g to 3 g and 100 g of sauce, respectively. The sensory evaluation is as follows: For the body taste (coarseness, spread, thickness) and aging, various commercially available products without seasoning liquid are used as controls (0 points). 0: None, 1: Slightly strong, 2: Strong, 3: Very good N = 1 0.
結果を表 7から表 10に示す。 The results are shown in Table 7 to Table 10.
表 7 Table 7
コント ールが市販そばつゆの場合の、 本発明品の添加効果  Effect of adding the product of the present invention when the soba soup is on the market
Figure imgf000018_0001
Figure imgf000018_0001
(※:カドとは、 風味や味をバラバラに感じることであり、 特有の風味や味を強 く感じてしまうことにより、 好ましくない風味 ·味となることを意味する) 表 8 (*: Kado means that the flavor and taste are felt apart, and that it makes the flavor and taste unfavorable by making the flavor and taste unique.) Table 8
コントロールが市販デミグラスソースの場合の、 本発明品の添加効果
Figure imgf000019_0001
3.0 2.9 2.2 3.0 2.5 醤油力ド抑えるので好ましい 表 7から表 10の官能評価結果から、 本発明品は各種食品において、 好ましくな いカドを抑えながら、 まとまり、 ひろがり、 厚みといったコク味をバランスよく付 与することにより、 好ましい熟成感を付与することが示された。
Effect of adding the product of the present invention when the control is a commercial demiglace sauce
Figure imgf000019_0001
3.0 2.9 2.2 3.0 2.5 It is preferable because it suppresses the soy sauce strength. From the sensory evaluation results in Table 7 to Table 10, the product of the present invention has a good balance of cohesiveness, spread, and thickness in various foods while suppressing undesirable cadence. It has been shown that a preferable aging feeling is imparted by giving.
(実施例 8 :各種濃度の本願発明調味料の飲食品への添加効果)  (Example 8: Effect of adding various concentrations of the present invention seasoning to food and drink)
実施例 6において予備加熱条件が 7 O , 2時間である調味料を「本発明品 6 (7 0 X 2) 」 とした。 市販のそばつゆ (ストレートタイプ 「そばつゆ」 ャマキ株式会 社製) に、 本発明品 6 (70 X 2) をそれぞれ 0. 0 1 g〜2 0 gZつゆ 1 0 0 g となるように添加したときの官能評価を行った (食塩分と水分は添加量が一番多い ものに合わせてそれぞれ調整した) 。 官能評価は、 コク味 (まとまり、 ひろがり、 厚み) 、 熟成感について、 調味液を添加しないそばつゆをコントロール (0点) と して、 0 :無い、 1 :やや強い、 2 :強い、 3 :非常に強い、 として、 n= 1 0で 行った。  In Example 6, the seasoning in which the preheating conditions were 7 O and 2 hours was defined as “Invention product 6 (70 × 2)”. Functionality when the product 6 (70 X 2) of the present invention 6 (70 X 2) is added to a commercially available soba soup (straight type “Soba soup” by Yamaki Co., Ltd.) so that it becomes 0.0 g to 20 g Z soup Evaluation was performed (salt content and water content were adjusted to the one with the highest amount added). The sensory evaluation is as follows: for richness (coarseness, spread, thickness) and aging, control soba soup without adding seasoning liquid (0 points), 0: none, 1: slightly strong, 2: strong, 3: very N = 1 0.
結果を表 1 1に示す。 The results are shown in Table 11.
表 1 1 Table 1 1
コントロールが市販そばつゆの場合の、 本発明品の各種濃度での添加効果  Effect of addition of various concentrations of the product of the present invention when the control is commercially available soba soup
Figure imgf000020_0001
1 2. 1 2. 2 2. 1 2. 1 まろやか、 濃厚感あり、 醤油力ド*塩力ド抑え好 ましい
Figure imgf000020_0001
1 2. 1 2. 2 2. 1 2. 1 Mild, rich, soy sauce *
5 2. 3 2. 2 2. 3 2. 3 まろやか、 濃厚感強い、 醤油力ド*塩力ド抑え好 ましい  5 2. 3 2. 2 2. 3 2. 3 Mild, strong and strong, soy sauce *
10 2. 7 2. 1 2. 8 2. 4 まろやか、 濃厚感強い、 醤油力ド*塩力ド抑え好 ましい  10 2. 7 2. 1 2. 8 2. 4 Mild, strong, soy sauce *
20 2. 8 2. 0 2. 9 2. 5 まろやか、 濃厚感強い、 醤油力ド'塩力ド抑え好 ましい  20 2. 8 2. 0 2. 9 2. 5 Mild, strong, strong soy sauce
(※ :カドとは、 風味や味をバラバラに感じることであり、 特有の風味や味を強  (*: Kado means that the flavor and taste are felt apart, and the unique flavor and taste are strong.
く感じてしまうことにより、 好ましくない風味 ·味となることを意味する) 表 1 1の官能評価結果から、 本発明品は食品において、 各種濃度で効果があるこ とが示された。 The result of sensory evaluation shown in Table 11 shows that the product of the present invention is effective at various concentrations in foods.
また好ましくは飲食品に対して調味料が 0 . 0 5 %以上 2 0 %以下となるように混 In addition, it is preferable that the seasoning is mixed with the food and drink so that the concentration is 0.05% to 20%.
合 ることができ、 さらに好ましくは飲食品に対して調味料が 0 . 1 %以上 5 %以 More preferably, the seasoning is 0.1% or more and 5% or less for food and drink.
下となるように混合することができることが示された。 産業上の利用可能性 ' It was shown that they can be mixed so that they are below. Industrial applicability ''
本発明によれば、 飲食品に高力価で良質な熟成感を付与できる調味料を提供する ことができる。  ADVANTAGE OF THE INVENTION According to this invention, the seasoning which can provide a high-quality and high quality aging feeling to food-drinks can be provided.

Claims

請 求 の 範 囲 The scope of the claims
1. 醤油類を除くアミノ酸源および またはペプチド源と糖源を酸化反応させる 工程を含む調味料の製造方法において、 酸化反応が酸素を含む気体の給気工程およ び/または撹拌工程を含み、 かつ酸化反応条件が 20で以上 5 Ot:以下、 0. 5時 間以上 48時間以下である事を特徴とする調味料の製造方法。 1. In a method for producing a seasoning comprising a step of oxidizing a amino acid source and / or a peptide source and a sugar source excluding soy sauce, the oxidation reaction includes a step of supplying a gas containing oxygen and / or a step of stirring, The method for producing a seasoning is characterized in that the oxidation reaction condition is 20 or more and 5 Ot: or less, and 0.5 hour or more and 48 hours or less.
2. アミノ酸源および またはペプチド源が、 脱色および Zまたは脱臭工程を経 て製造された酵母エキスまたはたん白加水分解物である請求項 1記載の調味料の製 造方法。  2. The method for producing a seasoning according to claim 1, wherein the amino acid source and / or peptide source is a yeast extract or a protein hydrolyzate produced through decolorization and Z or deodorization steps.
3. 酸素供給条件が、調味料 100 gあたり空気として給気量 10m 1 分以上、 300ml Z分以下、 給気時間 1時間以上 48時間以内である請求項 1記載の調味 料の製造方法。 3. The method for producing a seasoning according to claim 1, wherein the oxygen supply conditions are an air supply amount of 10 m 1 min or more, 300 ml Z min or less, and an air supply time of 1 hour to 48 hours per 100 g of seasoning.
4. 酸素供給条件が、 空気と接触する状態での撹拌下で、 調味料 l kgあたり撹 拌動力 0. 1W以上 200W以下である請求項 1記載の調味料の製造方法。  4. The method for producing a seasoning according to claim 1, wherein the oxygen supply condition is stirring power of 0.1 W to 200 W per kg of seasoning under stirring in contact with air.
5. アミノ酸源および Zまたはペプチド源に対して、 糖源との混合前に、 50 : 以上 100で以下、 0. 5時間以上 24時間以下の予備加熱を行うことを特徴とす る請求項 1ないし 4記載の調味料の製造方法。 5. The amino acid source and the Z or peptide source are preheated at a ratio of 50: 100 or less and 0.5 hours or more and 24 hours or less before mixing with the sugar source. Or 4. A method for producing the seasoning according to 4.
6. 請求項 1ないし請求項 5のいずれか 1項記載の方法で製造される調味料。 6. A seasoning produced by the method according to any one of claims 1 to 5.
7. 請求項 1ないし請求項 5のいずれか 1項記載の方法で製造される調味料を飲 食品に対して 0. 01 %以上 99 %以下となるように混合することを特徴とする飲 口 7. A mouthpiece characterized by mixing the seasoning produced by the method according to any one of claims 1 to 5 so as to be 0.01% or more and 99% or less of the food and drink.
8. 請求項 1ないし請求項 5のいずれか 1項記載の方法で製造される熟成感付与 剤。  8. A ripening sensation agent produced by the method according to any one of claims 1 to 5.
PCT/JP2009/056923 2008-03-31 2009-03-27 Method for production of flavoring material for imparting aged flavor WO2009123298A1 (en)

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JP2017023018A (en) * 2015-07-17 2017-02-02 味の素株式会社 Method of manufacturing seasoning

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JPWO2009123298A1 (en) 2011-07-28

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