WO2009121212A1

WO2009121212A1 - A detect system and method of the microoranism cell forms

Info

Publication number: WO2009121212A1
Application number: PCT/CN2008/000689
Authority: WO
Inventors: 孔兵; 杨宏伟; 范顺杰; 贺伯特·格里布; 卓越; 库特·贝腾豪森
Original assignee: 西门子公司
Priority date: 2008-04-03
Filing date: 2008-04-03
Publication date: 2009-10-08
Also published as: WO2009121212A8

Abstract

A detect system of the microorganism cell forms, including: a sample pushing element, streaming chamber, a optical detect element and a result creating element. The sample pushing element pushes the microorganism sample and the buffer into the streaming chamber. The buffer coats the microorganism sample in the streaming chamber, thus creating the sample sheath flow, making the microorganism cell in the sample flow through the optical micropath of the streaming chamber one by one. The optical detect element irradiates the microorganism cell one by one, and sends the light signal to the result creating element. The result creating element receives the light signal, decides the microorganism cell form by the light signal. And, a detect method of the microorganism cell forms. This subject can increase the accuracy of the microorganism cell form detection.

Description

Detection system and method for microbial cell morphology

Technical field

The present invention relates to microbial detection technology, and more particularly to a detection system and method for microbial cell morphology.

Background technique

In the process of cultivation or fermentation of microorganisms, single-cell microorganisms usually undergo several stages of changes. As shown in Fig. 1, Fig. 1 shows a schematic diagram of changes in various stages of microorganisms. Microorganisms first undergo an adaptation phase in which microorganisms adapt to the new environment and therefore grow slowly, at which point the number of cells grows slowly, even as the number remains constant, and the volume increases. This is followed by an exponential growth phase in which the microbe grows rapidly and splits into two small cells by one cell, after which each small cell grows into a large cell and then splits into two new small cells, the process repeats. The number of cells continues to increase. After a certain period of time, the microorganisms enter a stable phase in which the cells stop dividing and the number of cells no longer increases, but the cells may continue to metabolize. Finally, the microbe enters the death phase, in which water is lost through the cell membrane and causes the cells to shrink and eventually die.

In order to make better use of microbial fermentation to obtain an ideal fermentation product, it is necessary to control the cultivation and fermentation of microorganisms, for example, when to decide when to terminate the fermentation process, when to control the amount of microorganisms, and when to separate. Microorganisms, etc. The control process relies heavily on the measurement of processing parameters, especially microbial state parameters. Among the microbial status parameters, the number and morphology of microorganisms are the two most important parameters. In the prior art, the detection of the number and morphology of microorganisms is mainly based on the detection of a microscope. Since most microbial cells are translucent, the cells need to be chemically or physically colored prior to measurement to enhance contrast under the microscope. However, in this method, since the colored microorganisms usually have died, which is somewhat different from the living microorganisms in form or structure, the accuracy of detecting the microbial cell morphology is not high enough. In addition, it is difficult to obtain an accurate morphological detection result by manually identifying the shape by a microscope.

Summary of the invention In one aspect, the invention provides a detection system for microbial cell morphology, and another aspect provides: a method for detecting microbial cell morphology in order to improve the detection accuracy of microbial cell morphology.

The system for detecting the morphology of a microbial cell provided by the present invention comprises: a liquid propelling unit, a flow chamber, an optical measuring unit, and a result generating unit, wherein

a liquid propulsion unit for pushing the microbial sample solution and buffer into the flow chamber;

a flow chamber for encapsulating the buffer sample with the microbial sample fluid, forming a sheath flow intermediate the sample stream, and passing the microbial cells in the sample stream one by one through the optical measurement microchannel of the flow chamber; an optical measurement unit for Illuminating the optical signal of the micro-channel passing through the flow channel optical measurement microchannel, and transmitting the extracted optical signal to the result generating unit;

The result generating unit is configured to receive the optical signal, and determine a morphology of the microbial cell based on the optical signal.

Preferably, the optical measuring unit may include: a beam emitting unit, a second lens unit, a beam splitting unit, a first aperture penetrating unit, and a second diaphragm penetrating unit, wherein

The beam emitting unit is configured to emit an illuminating beam that is irradiated onto the microbial cells passing through the optical measuring microchannel of the flow chamber;

The second lens unit is configured to collect and collimate the scattered light generated by the microbial cells after being irradiated, and send the obtained parallel beam to the beam splitting unit;

The beam splitting unit is configured to separate the parallel beam into two parts, and send the separated two parts of the beam to the first aperture penetrating unit and the second aperture penetrating unit respectively; the pupil formed by the first set radius, Obtaining an optical signal reflecting a first set scattering angle of the microbial volume, and transmitting the optical signal to the result generating unit (240); and forming a second set radius to obtain a second set scattering angle reflecting the morphological complexity of the microbial cell The optical signal is sent to the result generating unit.

Preferably, the optical measuring unit further includes: a first lens unit, a third lens unit, and a fourth lens unit, wherein

a first lens unit for compressing an illumination beam from the beam emission unit, and irradiating the obtained compressed beam onto a microbial cell through which the flow chamber optical measurement microchannel passes; The third lens unit is configured to collect the optical signal of the first set scattering angle obtained by the first aperture transmitting unit, and then send the optical signal to the result generating unit;

The fourth lens unit is configured to collect the optical signal of the second set scattering angle obtained by the second pupil penetrating unit, and then send the signal to the result generating unit.

The result generating unit may include: a first photoelectric conversion unit, a second photoelectric conversion unit, and a scatter plot drawing unit, where

The first photoelectric conversion unit is configured to receive the optical signal of the first set scattering angle, convert the optical signal of the first set scattering angle into a first digital signal, and send the first digital signal to the first digital signal Point map drawing unit;

The second photoelectric conversion unit is configured to receive the optical signal of the second set scattering angle, convert the optical signal of the second set scattering angle into a second digital signal, and send the second digital signal to the second Point map drawing unit;

The scatter plot drawing unit is configured to generate a two-dimensional scattergram composed of the first digital signal and the second digital signal according to the received first digital signal and the second digital signal, and determine according to the scatter plot The morphology of microbial cells.

Preferably, the result generating unit may further include:

And a counting unit, configured to count pulses of the first digital signal or the second digital signal, and determine the number of microbial cells according to the counting result.

Wherein each of the first lens unit, the second lens unit, the third lens unit, and the fourth lens unit is: a single lens or a lens group.

Wherein, the lens is a spherical lens or a non-spherical lens.

Preferably, the first set scattering angle is an angle of 1 to 5 degrees or 2 to 5 degrees; and the second set scattering angle is an angle of 10 to 20 degrees or 80 to 100 degrees.

The method for detecting the morphology of a microbial cell provided by the invention comprises:

Pushing the microbial sample solution and buffer into the flow cell;

Circulating the microbial sample solution with the buffer, forming a sheath flow in the middle of the sample stream, and passing the microbial cells in the microbial sample stream one by one through the optical measurement microchannel of the flow chamber; Optically measuring microchannels of microbial cells for irradiation, and Determining the morphology of the microbial cells based on the optical signal

Preferably,

The optical signals of the cell morphology include:

Collecting and collimating the scattered light generated by the microbial cells after being irradiated to form a parallel beam;

Separating the parallel beam into a two-part beam, and transmitting part of the light of a part of the beam to the aperture formed according to the first set radius, to obtain an optical signal reflecting the first set scattering angle of the microbial volume, and the other part of the beam Part of the light passes through the aperture formed according to the second set radius, and an optical signal of a second set scattering angle reflecting the complexity of the morphology of the microbial cells is obtained.

Preferably, the determining the morphology of the microbial cell according to the optical signal comprises:

Converting the optical signal of the first set scattering angle into a first digital signal, and converting the optical signal of the second set scattering angle into a second digital signal;

And generating, according to the first digital signal and the second digital signal, a two-dimensional scattergram composed of the first digital signal and the second digital signal, and determining a morphology of the microbial cell according to the scattergram.

Preferably, the method further comprises: counting pulses of said first digital signal or said second digital signal, and determining the number of microbial cells based on said counting result.

The first set scattering angle is an angle of 1 to 5 degrees or 2 to 5 degrees; and the second set scattering angle is an angle of 10 to 20 degrees or 80 to 100 degrees.

It can be seen from the above scheme that in the present invention, the microbial sample liquid and the buffer solution for encapsulating the microbial sample liquid are pushed into the flow chamber, and the buffer solution is wrapped with the microbial sample liquid to form a sheath flow in the middle of the sample flow. The microbial cells in the sample stream are passed through the optical measurement microchannels of the flow cell one by one, and then each microbial cell that passes through the optical measurement microchannel is irradiated, and the morphology of the microbial cells is determined from the light signal. It can be seen that there is no need to color the microbial cells, and the microorganisms are not killed when the microbial cells are colored, but the microbial cells of the living body are directly detected, and the artificial detection is not required, thereby improving the detection accuracy of the microbial cell morphology. .

Further, in the present invention, the number of microbial cells can be determined according to the number of times of receiving the optical signal, so that the morphology and quantity of the microbial cells can be simultaneously obtained, and the optical signal generated after each irradiation of one cell is counted. , therefore the accuracy of detecting the number of microbial cells Got an improvement.

Moreover, since it is not necessary to color the microbial cells, the complexity of the detection is reduced, and the time taken for coloring the microbial cells is omitted, thereby reducing the detection time.

DRAWINGS

The above-described and other features and advantages of the present invention will become more apparent to those skilled in the <RTIgt

Figure 1 is a schematic diagram showing changes in various stages of microorganisms;

2 is an exemplary structural diagram of a microbial cell morphology detecting system according to an embodiment of the present invention; FIG. 3 is a diameter and a small scattering angle of a microbe corresponding spherical model obtained by using the MIE scattering principle according to an embodiment of the present invention; Schematic diagram of the relationship between the strength of the signal (Least angle scatter signal, LAS);

4(a) to 4(f) are schematic diagrams of scatter plots at different stages in an embodiment of the present invention;

Figure 5 is a schematic structural view of an optical measuring unit in the system shown in Figure 2;

6 is a schematic structural view of a pupil penetrating unit in the optical measuring unit shown in FIG. 5; FIG. 7 is a schematic structural view of a result generating unit in the system shown in FIG.

Figure 8 is an exemplary flow chart of a method for detecting the morphology of a microbial cell in an embodiment of the present invention.

detailed description

In the present invention, scattering is generated when light is irradiated onto the microbial cells, and scattered light generated by different cell morphology is also different. Therefore, in the embodiment of the present invention, the microbial cells can be irradiated with light, and An optical signal reflecting the morphology of the microbial cells is extracted from the generated scattered light, and the morphology of the microbial cells is determined based on the optical signal.

In order to perform statistical analysis on the morphology of each microbial cell, the microbial cells in the microbial sample can be irradiated one by one. In order to illuminate the microbial cells in the microbial sample one by one, the microbial sample can be passed through a special flow chamber. The flow chamber includes a microbial sample fluid inlet, a buffer (used as a sheath fluid for encapsulating the microbial sample), and a waste liquid outlet. In the middle of the flow chamber is a section of optical measuring microchannels that transmit light. Using fluid dynamics sheath flow technology, when the microbial sample liquid enters the flow chamber, it passes through the flow chamber under the buffer of the buffer, and makes the biological cells only one by one The microchannels are optically measured so that the light can illuminate the microbial cells passing through the microchannels one by one.

Further, based on the irradiation result, the number of microbial cells can be counted, and the number of microbial cells counted in a unit sample or per unit time can be calculated to determine the concentration of the microbial cells.

The present invention will be further described in detail below with reference to the accompanying drawings.

Fig. 2 is a view showing an exemplary configuration of a detection system of a microbial cell morphology in an embodiment of the present invention. As shown in FIG. 2, the system includes: a liquid propulsion unit 210, a flow chamber 220, an optical measurement unit 230, and a result generation unit 240.

The liquid propelling unit 210 is configured to push the microbial sample solution and the buffer for encapsulating the microbial sample solution into the flow chamber 220. In the specific implementation, it can be realized by a stepper motor driven syringe or by controlling a constant pressure system.

After entering the flow cell 220, the sample fluid is surrounded by a buffer to form a laminar flow (also called sheath flow) in the middle of the sample stream. The sample stream is further compressed as it passes through the optical measurement of the flow cell 220 such that the microbial cells in the sample stream pass one-by-one through the optical measurement microchannel of the flow cell 220, followed by the waste fluid consisting of the buffer and the microbial sample fluid from the flow cell Flowing out. Among them, the channel can be circular, or square or rectangular. For example, for a square channel, the side length can be taken from 0.1 mm to 0.2 mm.

The optical measuring unit 230 is configured to illuminate an optical signal of the microcell morphology that passes through the optical channel microchannels one by one, and transmits the extracted optical signals to the result generating unit 240.

The result generation unit 240 is configured to receive the optical signal, and determine the morphology of the microbial cells based on the optical signal.

Further, the result generation unit 240 may also determine the number of microbial cells based on the number of pulses receiving the optical signal.

In the specific implementation, the optical measurement unit 230 and the result generation unit 240 may have different structures and specific implementation forms according to different needs and different optical signals reflecting the morphology of the microbial cells.

In the embodiment of the present invention, the microbial cell is described as a ball according to the principle of MIE scattering. The body model, and uses a spheroid model to describe the scattering pattern of microbial cells, and then determine the morphology of the microbial cells. Through a large number of experimental simulations and data calculations, it is found that the Least Angle scatter signal (LAS), for example, the scattering angle is about 1 degree (.) to 5. Between, or 2. To 5. The scattering signal between them is sensitive to the diameter of the sphere model. As shown in Fig. 3, Fig. 3 is a schematic diagram showing the relationship between the diameter of the sphere model and the LAS intensity. It can be seen that the larger the diameter of the sphere model, the greater the strength of the LAS. Therefore, the volume morphology of the microbial cells can be indirectly expressed by the LAS intensity, that is, whether the microbial cells grow up.

In addition, a large number of experimental simulations and data calculations have also found that the Middle Angle scatter signal (MAS), for example, has a scattering angle of about 10° to 20. Between, or at 80. To 100. The scattered signal between them is sensitive to the complexity of cell morphology. Among them, the more complex the morphology of the cells, the greater the intensity of MAS. Therefore, the complexity of the microbial cell morphology can be indirectly expressed by the MAS intensity, that is, whether the microbial cell has a split or atrophic morphology or the like.

Therefore, a two-dimensional scattergram consisting of LAS and MAS intensity values can be drawn based on the LAS intensity and MAS intensity of each microbial cell in the current sample. The current morphology of the microorganism can be determined based on the intensity of the scattered light at different angles of the microorganisms in the scatter plot.

Figure 4(a) to Figure 4(f) show a schematic diagram of the scatter plots at different stages. In these figures, the MAS intensity is plotted on the abscissa, and in the illustration, the LAS intensity is the ordinate and the LAS intensity is the ordinate. As shown in Fig. 4(a) to Fig. 4(b), in the adaptation phase, the cell volume increases, causing the scatter plot to move upward, that is, the LAS intensity value becomes larger. As shown in Fig. 4(c), in the initial stage of the exponential growth phase, some cells begin to divide, causing the complexity of the cell morphology to increase accordingly, so the scatter plot moves to the right, that is, the MAS intensity value becomes larger. As shown in Fig. 4(d), as the exponential growth phase continues, various cells are present in the sample, including small cells, large cells, and cells during division, and correspondingly, a complex scatter plot of the distribution area appears. As shown in Figure 4(e), during the stabilization phase, most cells maintain a relatively fixed size and morphology, reforming a simple scatter plot of the distribution. As shown in Fig. 4(f), in the death phase, most of the dead cells only have a shrinking outer shell, and some living cells also begin to shrink a lot, that is, the distribution area shown in Fig. 4(f) moves to the lower left corner. Scatter plot.

As shown in FIG. 5, FIG. 5 is a schematic structural diagram of an optical measuring unit 230 according to an embodiment of the present invention. In order to make the correspondence clear, FIG. 5 also shows a schematic structural view of the flow chamber 220. As shown in FIG. 5, the optical measuring unit 230 includes a beam emitting unit 231, a first lens unit 232, a second lens unit 233, a beam splitting unit 234, a first pupil penetrating unit 235, and a second diaphragm penetrating unit 236. The third lens unit 237 and the fourth lens unit 238. The beam emitting unit 231 is configured to emit an illuminating beam, and the beam may be a laser beam or other beams.

The first lens unit 232 is for compressing the illumination beam and illuminating the resulting compressed beam onto the microbial cells through which the flow chamber 220 optically measures the microchannel. The energy of the illuminating beam can be enhanced by compression to increase the sensitivity of the detection.

The second lens unit 233 collects and collimates the scattered light generated by the compressed beam passing through the microbial cells, and transmits the obtained parallel beam to the beam splitting unit 234.

The beam splitting unit 234 is for separating the parallel beam into two parts, and transmits the separated two parts of the beam to the first pupil penetrating unit 235 and the second pupil penetrating unit 236, respectively. Wherein, when the scattered light beam is separated into two parts, the prism can be used for separation. An optical signal reflecting the first set scattering angle of the microbial volume is obtained by the aperture formed by the first set radius, and the optical signal of the first set scattering angle is transmitted to the third lens unit 237.

The third lens unit 237 is configured to aggregate the optical signals of the first set scattering angle and transmit the signals to the result generating unit 240. According to the aperture formed by the second set radius, an optical signal of a second set scattering angle reflecting the complexity of the microbial cell morphology is obtained, and the optical signal of the second set scattering angle is transmitted to the fourth lens unit 238.

The fourth lens unit 238 is configured to aggregate the optical signals of the second set scattering angle and transmit the signals to the result generating unit 240.

In this embodiment, the optical signal of the first set scattering angle may be LAS, and the optical signal of the second set scattering angle may be MAS; or the optical signal of the first set scattering angle may be MAS, the second set scattering The angle of the light signal can be LAS.

The structure of the first aperture penetrating unit 235 and the second diaphragm penetrating unit 236 may be as shown in FIG. 6, where η is the inner radius of the aperture, r ₂ is the outer radius of the aperture, and only between 1", and ₂ The aperture portion is light transmissive, and the other portions (the gray portion in the figure) are non-transmissive. The values of ^ and ^ can be determined according to the scattering angle of the optical signal to be obtained, for example, assuming the first set scattering angle The optical signal is LAS, and for the first aperture penetrating unit 235 that generates the LAS, it is assumed that the focal length of the third lens unit 237 is 30 mm, and the angular range of the LAS is 1. To 5°, then the calculation of "and" Formula can So:

r, = 30 · tan (l .) «0.52mm, r 2 = 30 · tan (5 °)« 2.62mm. In addition, the first lens unit 232, the second lens unit 233, the third lens unit 237, and the fourth lens unit 238 may select a single lens or a lens group according to the situation, and the lens may be a spherical lens or a non-spherical shape. lens.

The result of the embodiment of the present invention is that the optical signal reflecting the morphology of the microbial cell is an optical signal reflecting a first set scattering angle of the microbial volume and an optical signal of a second set scattering angle reflecting the complexity of the morphology of the microbial cell. The internal structure of the generating unit 240 can be as shown in FIG. 7. FIG. 7 is a schematic structural diagram of the result generating unit 240 according to the embodiment of the present invention. As shown in the solid line portion of Fig. 7, the result generating unit 240 includes: a first photoelectric conversion unit 241, a second photoelectric conversion unit 242, and a scattergram drawing unit 243.

The first photoelectric conversion unit 241 is configured to receive the optical signal of the first set scattering angle, convert the optical signal of the first set scattering angle into a first digital signal, and convert the first digital signal It is sent to the scatter plot drawing unit 243.

The second photoelectric conversion unit 242 is configured to receive the optical signal of the second set scattering angle, convert the optical signal of the second set scattering angle into a second digital signal, and send the second digital signal to A scatter plot drawing unit 243.

The scatter plot drawing unit 243 is configured to generate, according to the received first digital signal and the second digital signal, a two-dimensional scattergram composed of the first digital signal and the second digital signal, according to the scatter plot, Determine the morphology of the microbial cells.

When the first photoelectric conversion unit 241 and the second photoelectric conversion unit 242 are specifically implemented, the optical signal may be first converted into an analog electrical signal, such as a voltage signal, and then the analog electrical signal is subjected to A/D conversion and then converted. It is a digital signal, and thereafter, the digital signal is sent to the scatter plot drawing unit 243.

Further, as shown by the dotted line in FIG. 7, the result generating unit 240 may further include: a counting unit 244, configured to count pulses of the first digital signal or the second digital signal, according to the counting As a result, the number of microbial cells is determined.

The detection system of the microbial cell morphology in the embodiment of the present invention has been described in detail above, and the method for detecting the morphology of the microbial cells in the embodiment of the present invention will be described in detail below.

FIG. 8 is an exemplary flowchart of a method for detecting a morphology of a microbial cell according to an embodiment of the present invention. As shown in FIG. 8, the flow includes the following steps: In step 801, the microbial sample solution and the buffer for wrapping the biological sample solution are pushed into the flow chamber.

In specific implementation, the syringe can be driven by a stepper motor, or the microbial sample fluid and the buffer used to wrap the microbial sample fluid can be pushed into the flow chamber by controlling the constant pressure system.

Step 802, encapsulating the buffer with the biological sample liquid, forming a sheath flow with a sample flow in the middle, and passing the microbial cells in the microbial sample flow one by one through the optical measurement microchannel of the flow chamber

In this embodiment, the design of the flow cell can be consistent with the design of the flow cell in the system of Figure 2, and will not be described in detail herein.

Step 803, irradiating the microbial cells of the optical measuring microchannels one by one through the flow chamber, the light signal.

Similarly, in the embodiment of the method, the light signal reflecting the morphology of the microbial cell may be an optical signal reflecting a first set scattering angle of the microbial volume and a second set scattering angle reflecting a microbial cell morphology complexity. In this step, extracting the light signal reflecting the morphology of the microbial cells from the scattered light generated after the microbial cells are irradiated may include: collecting and collimating the scattered light generated by the microbial cells after being irradiated to form a parallel beam. Separating the parallel beam into two partial beams, and transmitting part of the light of a part of the beam to the pupil formed according to the first set radius, to obtain an optical signal reflecting the first set scattering angle of the microbial volume, and the other part of the beam Part of the light passes through the aperture formed according to the second set radius, and an optical signal of a second set scattering angle reflecting the complexity of the morphology of the microbial cells is obtained.

This step can be carried out by using the light detecting unit shown in FIG. 5 when it is specifically implemented.

Step 804, determining the morphology of the microbial cells according to the light signal reflecting the morphology of the microbial cells.

In the case where the optical signal reflecting the morphology of the microbial cell is an optical signal reflecting a first set scattering angle of the microbial volume and a second set scattering angle reflecting the complexity of the microbial cell morphology, in this step, The optical signal of the first set scattering angle is converted into a first digital signal, and the optical signal of the second set scattering angle is converted into a second digital signal. Then, a two-dimensional scattergram is generated according to the first pulse signal and the second pulse signal identified by the first digital signal and the second digital signal, and the morphology of the microbial cells is determined according to the scattergram. Further, in this embodiment, the number of microbial cells may also be determined according to the number of pulses receiving the optical signal. For the case where the optical signal reflecting the morphology of the microbial cell is an optical signal reflecting a first set scattering angle of the microbial volume and an optical signal of a second set scattering angle reflecting the complexity of the microbial cell morphology, the first The digital signal or the pulse of the second digital signal is counted, and based on the counting result, the number of microbial cells is determined.

In addition, the first set scattering angle in the embodiment may be an angle within a small angle scattering range, and the second set scattering angle may be an angle in the medium angle scattering range. Alternatively, the first set scattering angle may be an angle within a medium angle scattering range, and the second set scattering angle may be an angle within a small angular scattering range. Among them, the small angle scattering range is 1° to 5°, or 2° to 5°; the medium angle scattering range is 10° to 20°, or 80° to 100°.

The above description is only the preferred embodiment of the present invention and is not intended to limit the scope of the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and scope of the present invention are intended to be included within the scope of the present invention.

Claims

Rights request

A detection system for microbial cell morphology, characterized in that the system comprises: a liquid propulsion unit (210), a flow chamber (220), an optical measurement unit (230), and a result generation unit (240), wherein

a liquid propulsion unit (210) for pushing the microbial sample solution and buffer into the flow chamber (220);

a flow chamber (220) for encapsulating the buffer solution with the microbial sample fluid, forming a sheath flow intermediate the sample stream, and optically measuring the microbial cells in the sample stream one by one through the flow chamber (220) aisle;

The optical measuring unit (230) is configured to illuminate the microbial cells passing through the optical measuring microchannels of the flow chamber (220) one by one, and extract an optical signal reflecting the morphology of the microbial cells from the scattered light generated by the microbial cells after being irradiated , sending the extracted optical signal to the result generating unit (240);

The result generating unit (240) is configured to receive the optical signal, and determine a morphology of the micro-fine packet according to the optical signal.

2. The system according to claim 1, wherein the optical measuring unit (230) comprises: a beam emitting unit (231), a second lens unit (233), a beam splitting unit (234), a first aperture a penetrating unit (235) and a second aperture penetrating unit (236), wherein

The beam emitting unit (231) is configured to emit an illuminating beam that is irradiated onto the microbial cells passing through the optical measuring microchannel of the flow chamber (220);

The second lens unit (233) is configured to collect and collimate the scattered light generated by the microbial cells after being irradiated, and send the obtained parallel light beam to the beam splitting unit (234);

The beam splitting unit (234) is configured to separate the parallel beam into two parts, and separately send the separated two parts of the beam to the first aperture penetrating unit (235) and the second aperture penetrating unit (236);

The first pupil penetrating unit (235) is configured to pass a part of the received light beam through an aperture formed by the first set radius in the body, to obtain an optical signal reflecting the first set scattering angle of the microbial volume, Sending to the result generating unit (240);

a second aperture penetrating unit (236) for passing a portion of the received light beam through itself In the aperture formed according to the second set radius, an optical signal of a set scattering angle reflecting the complexity of the microbial cell morphology is obtained and sent to the result generating unit (240). .

3. The system according to claim 2, wherein the optical measuring unit (230) further comprises: a first lens unit (232), a third lens unit (237), and a fourth lens unit

( 238 ), where

a first lens unit (232) for compressing an illumination beam from the beam emitting unit (231), and irradiating the resulting compressed beam onto a microbial cell passing through an optical measurement microchannel of the flow chamber (220) ;

The third lens unit (237) is configured to collect the optical signal of the first set scattering angle obtained by the first aperture penetrating unit (235), and then send the signal to the result generating unit (240);

The fourth lens unit (238) is configured to collect the second set scattering angle optical signal obtained by the second aperture penetrating unit (236), and then transmit the optical signal to the result generating unit (240).

4. The system according to claim 3, wherein the result generating unit (240) comprises: a first photoelectric conversion unit (241), a second photoelectric conversion unit (242), and a scatter plot drawing unit (243) ), among them,

The first photoelectric conversion unit (241) is configured to receive the optical signal of the first set scattering angle, convert the optical signal of the first set scattering angle into a first digital signal, and convert the first digital signal Sended to a scatter plot drawing unit (243);

The second photoelectric conversion unit (242) is configured to receive the optical signal of the second set scattering angle, convert the optical signal of the second set scattering angle into a second digital signal, and convert the second digital signal Send to the scatter plot drawing unit (243);

a scatter plot drawing unit (243) for generating a two-dimensional scattergram composed of the first digital signal and the second digital signal according to the received first digital signal and the second digital signal, according to the scatter Figure, to determine the morphology of microbial cells.

The system according to claim 4, wherein the result generating unit (240) further comprises:

The counting unit (244) is configured to count pulses of the first digital signal or the second digital signal, and determine the number of microbial cells according to the counting result.

The system according to any one of claims 3 to 5, wherein the first lens unit (232), the second lens unit (233), the third lens unit (237), and the fourth lens Single Each lens element in element (238) is: a single lens or group of lenses.

7. The system of claim 6 wherein the lens is a spherical lens or an aspheric lens.

The system according to any one of claims 2 or 5, wherein the first set scattering angle is an angle of 1 to 5 degrees or 2 to 5 degrees; the second set scattering The angle is an angle of 10 to 20 degrees or 80 to 100 degrees.

9. A method for detecting a morphology of a biological cell, the method comprising: pushing a microbial sample solution and a buffer into a flow chamber;

Circulating the microbial sample solution with the buffer, forming a sheath flow in the middle of the sample stream, and passing the microbial cells in the microbial sample stream one by one through the optical measuring microchannel of the flow chamber;

Irradiating the microbial cells of the optical measuring microchannels one by one through the flow chamber, and from

Based on the light signal, the morphology of the microbial cells is determined.

10. The method according to claim 9, wherein extracting the optical signal reflecting the morphology of the microbial cell from the scattered light generated after the microbial cell is irradiated comprises:

Separating the parallel beam into a two-part beam, and transmitting a part of the light of a part of the beam to a pupil formed according to the first set radius to obtain an optical signal reflecting a first set scattering angle of the microbial volume, and another part of the beam Part of the light passes through the aperture formed according to the second set radius, and an optical signal of a second set scattering angle reflecting the complexity of the morphology of the microbial cells is obtained.

11. The method according to claim 10, wherein the determining the morphology of the microbial cell based on the optical signal comprises:

12. The method of claim 11, wherein the method further comprises: counting pulses of the first digital signal or the second digital signal, according to the counting knot If so, determine the number of microbial cells.

The method according to any one of claims 9 to 12, wherein the first set scattering angle is an angle of 1 to 5 degrees or 2 to 5 degrees; the second set scattering The angle is an angle of 10 to 20 degrees or 80 to 100 degrees.