WO2009121021A1 - Puces dégradables pour empêcher leur réutilisation - Google Patents

Puces dégradables pour empêcher leur réutilisation Download PDF

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Publication number
WO2009121021A1
WO2009121021A1 PCT/US2009/038669 US2009038669W WO2009121021A1 WO 2009121021 A1 WO2009121021 A1 WO 2009121021A1 US 2009038669 W US2009038669 W US 2009038669W WO 2009121021 A1 WO2009121021 A1 WO 2009121021A1
Authority
WO
WIPO (PCT)
Prior art keywords
probes
array
composition
nucleotide analog
nucleotide
Prior art date
Application number
PCT/US2009/038669
Other languages
English (en)
Inventor
Peter Welch
Original Assignee
Life Technologies Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corporation filed Critical Life Technologies Corporation
Publication of WO2009121021A1 publication Critical patent/WO2009121021A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Definitions

  • the present disclosure relates generally to nucleic acid arrays, and more specifically to nucleic acid arrays that can be treated to prevent reuse.
  • the present disclosure provides methods for degrading arrays.
  • the methods involve treating an array with a degradative agent, where one or more polynucleotide probes of the array include a nucleotide analog that is susceptible to chemical modification by the degradative agent.
  • the array may be a microarray and/or a spatially- addressable array.
  • the nucleotide analog may be a base-modified nucleotide (e.g., deoxyuridine monophosphate (dUMP) or deoxyuridine triphosphate (dUTP)).
  • the degradative agent may be a glycosylase (e.g., uracil DNA glycosylase).
  • One embodiment provided is a method of preparing an array in which the probes contain one or more nucleotide analogs, which upon modification prevent or minimize the ability to reuse the array.
  • the probes on the array are exposed to an buffer solution that is designed to facilitate the removal or degradation of the probes from the array.
  • the buffer solution may contain, for example, a degradative agent that interacts with and/or modifies at least some, and preferably substantially all, of the nucleotide analogs present in the probes.
  • uracil DNA glycosylase refers to any enzyme that catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. It will be appreciated that once sufficient degradation of the probes has been achieved, the probes can then be degraded from the array to prevent reuse of the array. In certain embodiments, the chemically modified probes are degraded and/or removed from the array using heat or protease treatment, or other methods well know to those of skill in the art. As discussed above, the degrading conditions are sufficiently stringent to remove substantially all or at least some portion of the probes from the array.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des puces pouvant être traitées pour empêcher leur réutilisation. Lesdites puces comprennent des sondes qui contiennent des analogues de nucléotide sensibles à un agent de dégradation. Après analyse, ces puces peuvent être traitées avec l’agent de dégradation, de manière à les rendre inadaptées pour une réutilisation. Par conséquent, la présente invention concerne des compositions et des procédés pour dégrader des sondes sur puce, de manière à empêcher la réutilisation de puce.
PCT/US2009/038669 2008-03-28 2009-03-27 Puces dégradables pour empêcher leur réutilisation WO2009121021A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4058208P 2008-03-28 2008-03-28
US61/040,582 2008-03-28

Publications (1)

Publication Number Publication Date
WO2009121021A1 true WO2009121021A1 (fr) 2009-10-01

Family

ID=40668171

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/038669 WO2009121021A1 (fr) 2008-03-28 2009-03-27 Puces dégradables pour empêcher leur réutilisation

Country Status (1)

Country Link
WO (1) WO2009121021A1 (fr)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4876187A (en) * 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
EP0624643A2 (fr) * 1993-05-11 1994-11-17 Becton, Dickinson and Company Décontamination de réactions d'amplification d'acides nucléiques
EP0742287A2 (fr) * 1995-05-10 1996-11-13 McGall, Glenn H. Sondes d'acide nucleique modifiés
WO1997041254A1 (fr) * 1996-04-30 1997-11-06 Ulf Landegren Detection amelioree d'acides nucleiques specifiques
WO1999007889A1 (fr) * 1997-08-05 1999-02-18 Ambion, Inc. Procedes et compositions de stripage d'acides nucleiques
WO2003083137A2 (fr) * 2002-03-26 2003-10-09 Amersham Biosciences Uk Limited Sondes immobilisees
WO2007030806A2 (fr) * 2005-09-09 2007-03-15 Combimatrix Corporation Procede de lavage d'un microreseau en vue d'une reutilisation de celui-ci
CA2564464A1 (fr) * 2005-10-13 2007-04-13 F. Hoffmann-La Roche Ag Epreuve multiple d'amplification d'acides nucleiques en phase solide

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4876187A (en) * 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
EP0624643A2 (fr) * 1993-05-11 1994-11-17 Becton, Dickinson and Company Décontamination de réactions d'amplification d'acides nucléiques
EP0742287A2 (fr) * 1995-05-10 1996-11-13 McGall, Glenn H. Sondes d'acide nucleique modifiés
WO1997041254A1 (fr) * 1996-04-30 1997-11-06 Ulf Landegren Detection amelioree d'acides nucleiques specifiques
WO1999007889A1 (fr) * 1997-08-05 1999-02-18 Ambion, Inc. Procedes et compositions de stripage d'acides nucleiques
WO2003083137A2 (fr) * 2002-03-26 2003-10-09 Amersham Biosciences Uk Limited Sondes immobilisees
WO2007030806A2 (fr) * 2005-09-09 2007-03-15 Combimatrix Corporation Procede de lavage d'un microreseau en vue d'une reutilisation de celui-ci
CA2564464A1 (fr) * 2005-10-13 2007-04-13 F. Hoffmann-La Roche Ag Epreuve multiple d'amplification d'acides nucleiques en phase solide

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DAHL FREDRIK ET AL: "Multigene amplification and massively parallel sequencing for cancer mutation discovery.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 29 MAY 2007, vol. 104, no. 22, 29 May 2007 (2007-05-29), pages 9387 - 9392, XP002530544, ISSN: 0027-8424 *

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