WO2009120049A2

WO2009120049A2 - Multichannel strip for a biosensor

Info

Publication number: WO2009120049A2
Application number: PCT/KR2009/001612
Authority: WO
Inventors: 정봉현; 유정민; 박혜정; 김상규
Original assignee: 한국생명공학연구원
Priority date: 2008-03-28
Filing date: 2009-03-30
Publication date: 2009-10-01
Also published as: KR20090009947U; KR200448186Y1; WO2009120049A3

Abstract

The invention relates to a multichannel strip for a biosensor, comprising: a lower substrate provided with a plurality of reaction portions, each having a defined region; and an upper substrate which comes in contact with the top of each of the plurality of reaction portions and is provided with a plurality of sample inlets and air outlets. The multichannel strip may be effectively used for the assay of various samples and for the diagnosis of all kinds of diseases or the like because it can analyze a number of materials concurrently, has a simple configuration, is easy to produce and can receive a small amount of sample without any pretreatment.

Description

Multichannel Strips for Biosensors

The present invention relates to a multi-channel strip for a biosensor, and more particularly, to analyze a plurality of in vivo materials at once, including a plurality of reaction sections and a plurality of sample introduction sections in one strip. The present invention relates to a multichannel strip for a biosensor.

Biosensors can be described as "a system that uses biological elements or mimics biological elements when obtaining information from an object to be converted into useful signals that can be recognized, such as color, fluorescence, and electrical signals." Therefore, biosensor is a future fusion technology that requires science-wide technologies such as electronics, chemistry, biology, materials engineering, and enzyme engineering.

The main fields of use for biosensors are medical, environmental, food, industrial, military, and experimental research. Among them, medical is the most widely used field, and most of the market size is mainly biosensor for glucose measurement.

Biosensors have a small amount of required sample, high accuracy of measurement, low influence of surrounding environment, easy measurement and real time measurement. In addition, it is a product that is in the early stage of market entry in terms of market, and has a variety of fields of use and is expected to have high growth potential.

The recent research and development trend is focused on the development of biosensors and disposable biosensors using a minimum amount of samples, and a lot of researches on the technical aspects of biosensor manufacturing to achieve this purpose have been conducted. .

Looking at the structural aspects of the biosensor, examples of the sample introduction portion of the conventional biosensor can be largely classified into | -shaped and-shaped.

The sample introduction of the shaped structure has a structure in which a hole is formed at the end of the straight sample introduction passage to form a capillary tube. However, the sample introduction portion of the | -shaped structure has a disadvantage in that the amount of the injected sample may change due to the phenomenon that the end portion of the passage is not completely filled or overflows depending on the viscosity of the sample injected through the sample introduction passage. In addition, it can be used only to analyze a single feature, and when a single biosensor strip is provided with multiple channels, the samples are mixed, which makes it difficult to measure each channel.

On the other hand, the sample introduction portion of the? -Shaped structure has a structure that forms a straight passage from the side of the sensor to the opposite side. Such ㅡ shaped sample introduction part has the inconvenience of introducing the sample from the side, and it is difficult to use in strips for biosensors having multiple channels like the ㅣ shaped sample introduction part.

In addition, in the case of the strip for conventional biosensors, since a separate air discharge tunnel is not directly connected to the outside of the strip from the reaction section, the analysis time takes a lot of time because the sample is introduced into the reaction section and the rate of diffusion is slow. .

Accordingly, the present inventors have studied in view of the above point, and the lower substrate having a plurality of reaction portions separated from each other and the upper substrate abuts with a portion of the upper portion of each of the plurality of reaction portions, the upper substrate having a sample introduction portion It is possible to simultaneously analyze a plurality of materials through a plurality of reaction units, the structure is simple, easy to manufacture, it is possible to manufacture a multi-channel strip for biosensors that can introduce a small amount of sample without pretreatment process. It was confirmed that the present invention was completed.

The multi-channel strip for the biosensor of the present invention can analyze a plurality of materials at the same time in a plurality of reaction units, the structure is simple, easy to manufacture, and a small amount of samples can be introduced without pretreatment, and various samples It can be used efficiently for the analysis and diagnosis of various diseases.

1 is a three-dimensional view of a multichannel strip for a biosensor.

2 is a diagram of a multichannel strip for a biosensor.

1: bottom substrate

2: upper substrate

3: cover

4: hydrophobic adhesive film

21: division of reaction part

22, 23: sample inlet or air outlet

3 is a diagram of a multi-channel strip for a biosensor, characterized in that the tip of the sample introduction section extends orthogonally so as to open toward the lower substrate. The sample inlet 24 refers to the upper end of the sample introduction section.

10: lower substrate

20: upper substrate

24: sample inlet

40: hydrophobic adhesive film

In one aspect, the present invention,

(a) a lower substrate having a plurality of reaction parts separated from one another; And

(b) an upper substrate in contact with an upper portion of each of the plurality of reaction portions, the upper substrate having a plurality of sample introduction portions and an air discharge portion;

It provides a multi-channel strip for a biosensor comprising a.

Hereinafter, with reference to the accompanying drawings, the present invention will be described in detail.

The lower substrate 1 having a plurality of reaction parts in which the regions of (a) of the present invention are separated from each other includes at least one, preferably 2 to 100, more preferably 2 to 10 electrodes. And reacting with the analyte to form on the electrode.

The substrate may include a working electrode, a reference electrode, and / or a counter electrode, similarly to a biosensor for measuring analytes in a sample.

The working electrode can be fabricated using carbon, graphite, platinum treated carbon, silver, gold, palladium or platinum components. For example, the working electrode can be printed on the lower substrate using an ink consisting of carbon or platinum treated carbon, or an ink comprising palladium. Alternatively, the working electrode may be formed on the lower substrate by vacuum deposition using gold.

In addition, the lower substrate of (a) may include a hydrophobic adhesive film 4 on the top in addition to the plurality of reaction parts. It is necessary to impart insulation to the lower substrate, and preferably can be prepared by selecting from the group consisting of polyester, poly vinyl chloride, polycarbonate, and mixtures thereof.

For example, the reaction unit may generate a reaction such as the following reaction formula. The reduced electron transfer mediator generated by the reaction between the substrate and the enzyme oxidizes the electron transfer mediator in the reduced state by applying a constant voltage to the working electrode based on the reference electrode or the auxiliary electrode. The analyte in the sample can be quantified by measuring.

Substrate + enzyme (oxidation state) + electron transfer mediator (oxidation state) ===> Reactant + enzyme (oxidation state) + electron transfer mediator (reduction state)

In the present invention, various in vivo materials may be used as analytes, and examples thereof include enzymes and metabolites in vivo. The enzymes include oxidoreductases such as glucose oxidase, lactate oxidase, cholesterol oxidase and alcohol oxidase; Glucose dehydrogenase, GOT (glutamate oxaloacetate transmianse) and GPT (glutamate pyruvate transmianse), such as transferases and hydrolases, and the like, the metabolites include cholesterol, lactate, creatinine, alcohol, blood sugar and the like. One preferred example is the use of GOT and GPT simultaneously.

The amount of sample that can be used in the strip according to the invention is small, preferably 1 to 10 μl may be used.

In contact with the upper portion of each of the plurality of reaction portions of the above (b) of the present invention, provided with a plurality, preferably 2 to 100, more preferably 2 to 10 sample introduction portions (22, 23) The upper substrate 2 protects the electrode and the reaction section is divided 21.

A plurality of samples may be introduced through the plurality of sample introduction parts, and the reaction parts may be completely separated by the upper substrate 2, and thus, the plurality of samples may not be mixed, thereby enabling accurate analysis.

In addition, the

air outlets

22 and 23 are provided so that when the sample is introduced into the reaction unit, the air filled in the reaction unit through the air discharge unit is introduced into the reaction unit while the sample introduced through the sample inlet enters the reaction unit. As it is discharged to the sample suction speed to the reaction unit can be further improved. That is, the strip of the present invention has the advantage of applying a rapid suction speed and a constant amount of sample by sample injection using a simple capillary phenomenon without using an absorbent pad.

The sample introduction part may be located in both the transverse direction 22 and the longitudinal direction 23, and when manufactured thinner and longer, the injection efficiency is better. If one direction is used as the sample introduction part, the other direction is used as the air outlet part. Therefore, the sample introduction portion and the air discharge portion of the present invention "b" or "a" shape, or the bent portion in the shape is 90 ° or less, preferably 30 ° to 90 °, more preferably 60 ° to 90 It has a shape consisting of an angle of °.

In addition, the sample introduction portion of the present invention can be orthogonally extended such that its tip is open toward the lower substrate 10, as shown in FIG. At this time, the sample inlet 24, which is a portion into which the sample is injected into the upper end portion of the sample introduction unit, is present in the upper substrate 20.

The upper substrate may preferably be prepared from the group consisting of polyesters, polyvinylchlorides, polycarbonates and mixtures thereof.

Additionally, in the present invention, in addition to the above (a) and (b) it may include a cover (3). The cover protects the sample inlet from the outside to prevent evaporation or loss of each substrate fixed to the reaction unit and the introduced sample, thereby preventing their concentration change. This makes it possible to further improve the accuracy of the analysis.

The cover can be made of any material commonly used, and preferably made of polyester, polyvinylchloride, polycarbonate.

The multi-channel strip for biosensor of the present invention is capable of analyzing a plurality of materials at the same time, has a simple structure, is easy to manufacture, and a small amount of samples can be introduced without pretreatment. It can be used efficiently for the diagnosis of

Claims

(a) a lower substrate having a plurality of reaction parts separated from one another; And

(b) an upper substrate in contact with a portion of an upper portion of each of the plurality of reaction portions, the upper substrate having a plurality of sample introduction portions and an air discharge portion;

Multi-channel strip for biosensor comprising a.
The multi-channel strip for biosensor according to claim 1, further comprising a cover in addition to the substrates of (a) and (b).
The multi-channel strip for biosensor according to claim 1, wherein the sample introduction part and the air discharge part of (b) have a "b" or "a" shape.
The multi-channel strip for biosensor of claim 3, wherein the “b” or “a” shaped bends have an angle of 90 ° or less.
The multi-channel strip for biosensor according to claim 1, wherein the sample introduction portion of (b) extends perpendicularly so that its tip is opened toward the lower substrate.
The multi-channel strip for biosensor according to claim 1 or 5, wherein the lower substrate of (a) includes a hydrophobic adhesive film on the top in addition to the plurality of reaction parts.
The biosensor of claim 6, wherein the hydrophobic adhesive film is selected from the group consisting of polyester, polyvinyl chloride, polycarbonate, and mixtures thereof. For multichannel strips.
The multi-channel strip for biosensor according to claim 1 or 5, wherein the plurality of reaction parts are 2 to 100.
The multi-channel strip for biosensor according to claim 8, wherein the plurality of reaction parts is 2 to 10.
The multichannel strip for biosensor according to claim 1 or 5, wherein the analyte of the multisensor strip for biosensor is an in vivo material.
The multi-channel strip for biosensor according to claim 10, wherein the substance in vivo is an enzyme or metabolite in vivo.
12. The multichannel strip for biosensor according to claim 11, wherein the enzyme is selected from the group comprising oxidoreductase, transferase and hydrolase.
12. The multi-channel strip for biosensor according to claim 11, wherein the metabolite is selected from the group consisting of cholesterol, lactate, creatinine, alcohol and blood sugar.
11. The multichannel strip of biosensor according to claim 10, wherein said in vivo materials are GOT and GPT.
6. The multi-channel strip for biosensor according to claim 1 or 5, wherein the upper substrate of (b) is selected from the group consisting of polyester, polyvinyl chloride, polycarbonate, and mixtures thereof.
The multi-channel strip for biosensor according to claim 1 or 5, wherein the sample introduction part (b) is 2 to 100.
The method according to claim 16, wherein the sample introduction of (b) Multichannel strip for biosensor, characterized in that 2 to 10.
The multi-channel strip for biosensor according to claim 1 or 5, wherein the amount of the sample injected through the sample introduction part of (b) is 1 to 10 µl.