WO2009117138A3 - Counterstaining method for dual-stain in situ hybridization - Google Patents

Counterstaining method for dual-stain in situ hybridization Download PDF

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Publication number
WO2009117138A3
WO2009117138A3 PCT/US2009/001758 US2009001758W WO2009117138A3 WO 2009117138 A3 WO2009117138 A3 WO 2009117138A3 US 2009001758 W US2009001758 W US 2009001758W WO 2009117138 A3 WO2009117138 A3 WO 2009117138A3
Authority
WO
WIPO (PCT)
Prior art keywords
situ hybridization
stain
dual
counterstaining
counterstaining method
Prior art date
Application number
PCT/US2009/001758
Other languages
French (fr)
Other versions
WO2009117138A2 (en
Inventor
Marc E. Key
Original Assignee
Spring Bioscience Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spring Bioscience Corporation filed Critical Spring Bioscience Corporation
Publication of WO2009117138A2 publication Critical patent/WO2009117138A2/en
Publication of WO2009117138A3 publication Critical patent/WO2009117138A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The disclosure relates to methods of practicing immunohistochemistry, and in situ hybridization analysis.
PCT/US2009/001758 2008-03-21 2009-03-19 Counterstaining method for dual-stain in situ hybridization WO2009117138A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US7032808P 2008-03-21 2008-03-21
US7043908P 2008-03-21 2008-03-21
US7029308P 2008-03-21 2008-03-21
US61/070,328 2008-03-21
US61/070,293 2008-03-21
US61/070,439 2008-03-21

Publications (2)

Publication Number Publication Date
WO2009117138A2 WO2009117138A2 (en) 2009-09-24
WO2009117138A3 true WO2009117138A3 (en) 2010-01-07

Family

ID=41091452

Family Applications (3)

Application Number Title Priority Date Filing Date
PCT/US2009/001760 WO2009117140A2 (en) 2008-03-21 2009-03-19 Method of performing high density multiplex staining for in situ hybridization
PCT/US2009/001758 WO2009117138A2 (en) 2008-03-21 2009-03-19 Counterstaining method for dual-stain in situ hybridization
PCT/US2009/001759 WO2009117139A2 (en) 2008-03-21 2009-03-19 Dual method for performing in situ hybridization

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2009/001760 WO2009117140A2 (en) 2008-03-21 2009-03-19 Method of performing high density multiplex staining for in situ hybridization

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US2009/001759 WO2009117139A2 (en) 2008-03-21 2009-03-19 Dual method for performing in situ hybridization

Country Status (1)

Country Link
WO (3) WO2009117140A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10041950B2 (en) 2012-03-27 2018-08-07 Ventana Medical Systems, Inc. Signaling conjugates and methods of use
US9970847B2 (en) * 2012-03-30 2018-05-15 Konica Minolta, Inc. Method for staining tissue
AU2016248882B2 (en) * 2015-04-13 2020-04-30 Ventana Medical Systems, Inc. Thermochemical-based antibody inactivation methods and systems
KR20180110094A (en) 2016-02-08 2018-10-08 루나포어 테크놀로지스 에스에이 Sample Cycle Multiplexing and Real-Time Imaging Method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6066459A (en) * 1993-08-18 2000-05-23 Applied Spectral Imaging Ltd. Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding
US6573043B1 (en) * 1998-10-07 2003-06-03 Genentech, Inc. Tissue analysis and kits therefor

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6066459A (en) * 1993-08-18 2000-05-23 Applied Spectral Imaging Ltd. Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding
US6573043B1 (en) * 1998-10-07 2003-06-03 Genentech, Inc. Tissue analysis and kits therefor

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
D. K. CHELVANAYAGAM ET AL.: "Toluidine blue-O is a Nissl bright-field counterstain for lipophilic fluorescent tracers Di-ASP, DiI and DiO.", IN J. OF NEUROSCIENCE METHODS., vol. 72, 1997, pages 49 - 55 *
K. BINK ET AL.: "TO-PRO-3 is an optimal fluorescent dye for nuclear counterstaining in dual- colour FISH on paraffin sections.", HISTOCHEM. CELL BIOL., vol. 115, 2001, pages 293 - 299 *

Also Published As

Publication number Publication date
WO2009117139A3 (en) 2009-11-19
WO2009117139A2 (en) 2009-09-24
WO2009117138A2 (en) 2009-09-24
WO2009117140A2 (en) 2009-09-24
WO2009117140A3 (en) 2009-11-12

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