WO2009103978A3 - Biological materials and uses thereof - Google Patents

Biological materials and uses thereof Download PDF

Info

Publication number
WO2009103978A3
WO2009103978A3 PCT/GB2009/000455 GB2009000455W WO2009103978A3 WO 2009103978 A3 WO2009103978 A3 WO 2009103978A3 GB 2009000455 W GB2009000455 W GB 2009000455W WO 2009103978 A3 WO2009103978 A3 WO 2009103978A3
Authority
WO
WIPO (PCT)
Prior art keywords
spermatozoan
transgenic
nucleic acid
acid molecule
viral vector
Prior art date
Application number
PCT/GB2009/000455
Other languages
French (fr)
Other versions
WO2009103978A2 (en
Inventor
Anil Chandrashekran
Robert Winston
Carol Winifred Readhead
Original Assignee
Atazoa Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Atazoa Limited filed Critical Atazoa Limited
Publication of WO2009103978A2 publication Critical patent/WO2009103978A2/en
Publication of WO2009103978A3 publication Critical patent/WO2009103978A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

Abstract

The present invention relates to a method for incorporating a nucleic acid molecule of interest into a mature spermatozoan so as to obtain a transgenic spermatozoan comprising exposing a mature spermatozoan of a vertebrate to a pseudotyped viral vector comprising the nucleic acid molecule of interest under conditions which allow for the introduction of said pseudotyped viral vector into the spermatozoan. Transgenic spermatozoa obtained by the method may be used to obtain transgenic offspring, for example, to correct genetic disorders or provide therapeutic proteins.
PCT/GB2009/000455 2008-02-19 2009-02-19 Biological materials and uses thereof WO2009103978A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0802980A GB0802980D0 (en) 2008-02-19 2008-02-19 Biological materials and uses thereof
GB0802980.3 2008-02-19

Publications (2)

Publication Number Publication Date
WO2009103978A2 WO2009103978A2 (en) 2009-08-27
WO2009103978A3 true WO2009103978A3 (en) 2009-12-10

Family

ID=39271906

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2009/000455 WO2009103978A2 (en) 2008-02-19 2009-02-19 Biological materials and uses thereof

Country Status (2)

Country Link
GB (1) GB0802980D0 (en)
WO (1) WO2009103978A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108835049B (en) * 2018-08-11 2021-04-06 辽宁省农业科学院 Method for breeding self-identification male and female geese

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000069257A2 (en) * 1999-05-13 2000-11-23 Cedars-Sinai Medical Center Genetic modification of male germ cells for generation of transgenic species and genetic therapies
WO2003056022A2 (en) * 2001-12-21 2003-07-10 Oxford Biomedica (Uk) Limited Method for producing a transgenic organism using a lentiviral expression vector such as eiav

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000069257A2 (en) * 1999-05-13 2000-11-23 Cedars-Sinai Medical Center Genetic modification of male germ cells for generation of transgenic species and genetic therapies
WO2003056022A2 (en) * 2001-12-21 2003-07-10 Oxford Biomedica (Uk) Limited Method for producing a transgenic organism using a lentiviral expression vector such as eiav

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KALINA J ET AL: "Retrovirus-mediated in vitro gene transfer into chicken male germ line cells", REPRODUCTION, BIOSCIENTIFICA LTD, GB, vol. 134, no. 3, 1 September 2007 (2007-09-01), pages 445 - 453, XP008086094, ISSN: 1470-1626 *
NAGANO M ET AL: "Retrovirus-mediated gene delivery into male germ line stem cells", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 475, no. 1, 9 June 2000 (2000-06-09), pages 7 - 10, XP004337222, ISSN: 0014-5793 *
SAKAI ET AL: "In vitro male germ cell cultures of zebrafish", METHODS : A COMPANION TO METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC., NEW YORK, NY, US, vol. 39, no. 3, 1 July 2006 (2006-07-01), pages 239 - 245, XP024908461, ISSN: 1046-2023, [retrieved on 20060701] *

Also Published As

Publication number Publication date
GB0802980D0 (en) 2008-03-26
WO2009103978A2 (en) 2009-08-27

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