WO2009093813A3 - Methods for electrically detecting interactions between biomolecules using scanning tunneling microscope - Google Patents

Methods for electrically detecting interactions between biomolecules using scanning tunneling microscope Download PDF

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Publication number
WO2009093813A3
WO2009093813A3 PCT/KR2008/007655 KR2008007655W WO2009093813A3 WO 2009093813 A3 WO2009093813 A3 WO 2009093813A3 KR 2008007655 W KR2008007655 W KR 2008007655W WO 2009093813 A3 WO2009093813 A3 WO 2009093813A3
Authority
WO
WIPO (PCT)
Prior art keywords
biomolecule
biomolecules
present
stm
interaction
Prior art date
Application number
PCT/KR2008/007655
Other languages
French (fr)
Other versions
WO2009093813A2 (en
Inventor
Jeong-Woo Choi
Byung-Keun Oh
Woochang Lee
Da-Yeon Kang
Original Assignee
Industry-University Cooperation Foundation Sogang University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Industry-University Cooperation Foundation Sogang University filed Critical Industry-University Cooperation Foundation Sogang University
Publication of WO2009093813A2 publication Critical patent/WO2009093813A2/en
Publication of WO2009093813A3 publication Critical patent/WO2009093813A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
    • G01Q60/00—Particular types of SPM [Scanning Probe Microscopy] or microscopes; Essential components thereof
    • G01Q60/10—STM [Scanning Tunnelling Microscopy] or apparatus therefor, e.g. STM probes
    • G01Q60/14—STP [Scanning Tunnelling Potentiometry]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
    • G01Q60/00—Particular types of SPM [Scanning Probe Microscopy] or microscopes; Essential components thereof
    • G01Q60/10—STM [Scanning Tunnelling Microscopy] or apparatus therefor, e.g. STM probes
    • G01Q60/12—STS [Scanning Tunnelling Spectroscopy]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/26—Electron or ion microscopes; Electron or ion diffraction tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y35/00—Methods or apparatus for measurement or analysis of nanostructures

Abstract

The present invention relates to a method for electrically detecting an interaction between biomolecules using a scanning tunneling microscope (STM), comprising the steps of: (a) immobilizing a first biomolecule on a metal substrate; (b) applying a second biomolecule to the metal substrate to induce the interaction between the first biomolecule and the second biomolecule; and (c) scanning the metal substrate using STM to measure an electric signal, whereby the occurrence of the interaction between the first and the second biomolecules is determined. The present STM-based electrical detection method enables to detect analytes having much lower concentrations {e.g., 100 fg/mL). In addition, the method of the present invention allows multiple detections in a single measurement system {e.g., single protein chip). On the other hand, the present invention permits to successfully analyze a nano-size array of which detection is impossible in a conventional method based on the fluorescence and allows ultrahigh sensitivity by signal enhancement through silver staining of Au nanoparticles.
PCT/KR2008/007655 2007-12-28 2008-12-24 Methods for electrically detecting interactions between biomolecules using scanning tunneling microscope WO2009093813A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2007-0140445 2007-12-28
KR1020070140445A KR100981987B1 (en) 2007-12-28 2007-12-28 Methods for Electrically Detecting Interactions between Biomolecules Using Scanning Tunneling Microscope

Publications (2)

Publication Number Publication Date
WO2009093813A2 WO2009093813A2 (en) 2009-07-30
WO2009093813A3 true WO2009093813A3 (en) 2009-10-08

Family

ID=40901524

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2008/007655 WO2009093813A2 (en) 2007-12-28 2008-12-24 Methods for electrically detecting interactions between biomolecules using scanning tunneling microscope

Country Status (2)

Country Link
KR (1) KR100981987B1 (en)
WO (1) WO2009093813A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101394200B1 (en) 2012-08-22 2014-05-14 주식회사 서린바이오사이언스 Silver nanocluster probe, method for detecting target polynucleotide using the same, and method for designing silver nanocluster probe

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030134273A1 (en) * 2001-07-17 2003-07-17 Eric Henderson Combined molecular binding detection through force microscopy and mass spectrometry
US20060035234A1 (en) * 2003-01-02 2006-02-16 Eric Henderson Method and apparatus for molecular analysis in small sample volumes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030134273A1 (en) * 2001-07-17 2003-07-17 Eric Henderson Combined molecular binding detection through force microscopy and mass spectrometry
US20060035234A1 (en) * 2003-01-02 2006-02-16 Eric Henderson Method and apparatus for molecular analysis in small sample volumes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SAUTHIER, M. L. ET AL.: "Nanoparticle Layers Assembled through DNA Hybridization: Characterization and Optimization", LANGMUIR, vol. 18, no. 5, 2002, pages 1825 - 1830 *
WANG, J. ET AL.: "Silver-Enhanced Imaging of DNA Hybridization at DNA Microarrays with Scanning Electrochemical Microscopy", LANGMUIR, vol. 18, 2002, pages 6653 - 6658 *

Also Published As

Publication number Publication date
KR20090072356A (en) 2009-07-02
WO2009093813A2 (en) 2009-07-30
KR100981987B1 (en) 2010-09-17

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