WO2009093022A3 - Cell re-programming - Google Patents

Cell re-programming Download PDF

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Publication number
WO2009093022A3
WO2009093022A3 PCT/GB2009/000164 GB2009000164W WO2009093022A3 WO 2009093022 A3 WO2009093022 A3 WO 2009093022A3 GB 2009000164 W GB2009000164 W GB 2009000164W WO 2009093022 A3 WO2009093022 A3 WO 2009093022A3
Authority
WO
WIPO (PCT)
Prior art keywords
programming
cell
stem cell
cell phenotype
cancer
Prior art date
Application number
PCT/GB2009/000164
Other languages
French (fr)
Other versions
WO2009093022A2 (en
Inventor
Jie Na
Peter Andrews
Original Assignee
University Of Sheffield
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Sheffield filed Critical University Of Sheffield
Publication of WO2009093022A2 publication Critical patent/WO2009093022A2/en
Publication of WO2009093022A3 publication Critical patent/WO2009093022A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0695—Stem cells; Progenitor cells; Precursor cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/30—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from cancer cells, e.g. reversion of tumour cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Transplantation (AREA)
  • Oncology (AREA)

Abstract

We disclose methods to re-programme differentiated somatic cells to a stem cell phenotype and also the de-differentiation of cancer cells to a cancer stem cell phenotype.
PCT/GB2009/000164 2008-01-23 2009-01-22 Cell re-programming WO2009093022A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0801215A GB0801215D0 (en) 2008-01-23 2008-01-23 Cell re-programming
GB0801215.5 2008-01-23

Publications (2)

Publication Number Publication Date
WO2009093022A2 WO2009093022A2 (en) 2009-07-30
WO2009093022A3 true WO2009093022A3 (en) 2009-11-12

Family

ID=39166223

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2009/000164 WO2009093022A2 (en) 2008-01-23 2009-01-22 Cell re-programming

Country Status (2)

Country Link
GB (1) GB0801215D0 (en)
WO (1) WO2009093022A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9012219B2 (en) 2005-08-23 2015-04-21 The Trustees Of The University Of Pennsylvania RNA preparations comprising purified modified RNA for reprogramming cells
EP4332227A1 (en) 2005-08-23 2024-03-06 The Trustees of the University of Pennsylvania Rna containing modified nucleosides and methods of use thereof
EP2881461A1 (en) * 2008-11-21 2015-06-10 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Reprogramming cells toward a pluripotent state
EP2474610B1 (en) 2009-08-31 2016-08-03 Osaka University Method for efficient production of induced pluripotent stem cells utilizing cells derived from gingival fibroblasts
AU2015215938B2 (en) * 2009-12-07 2018-01-18 The Trustees Of The University Of Pennsylvania RNA preparations comprising purified modified RNA for reprogramming cells
KR102171849B1 (en) * 2009-12-07 2020-10-30 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 Rna preparations comprising purified modified rna for reprogramming cells
WO2011130624A2 (en) * 2010-04-16 2011-10-20 Immune Disease Institute, Inc. Sustained polypeptide expression from synthetic, modified rnas and uses thereof
WO2011148983A1 (en) * 2010-05-25 2011-12-01 独立行政法人国立がん研究センター Induced malignant stem cells or pre-induction cancer stem cells capable of self-replication outside of an organism, production method for same, and practical application for same
US9228204B2 (en) 2011-02-14 2016-01-05 University Of Utah Research Foundation Constructs for making induced pluripotent stem cells
TW201335372A (en) * 2011-11-30 2013-09-01 Nat Cancer Ct Induced malignant stem cell
SI3421601T1 (en) 2011-12-30 2020-03-31 Cellscript, Llc Making and using in vitro-synthesized ssrna for introducing into mammalian cells to induce a biological or biochemical effect
WO2013177133A2 (en) 2012-05-21 2013-11-28 The Regents Of The Univerisity Of California Generation of human ips cells by a synthetic self- replicative rna

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070087437A1 (en) * 2005-10-14 2007-04-19 Jifan Hu Methods for rejuvenating cells in vitro and in vivo
WO2008097926A2 (en) * 2007-02-02 2008-08-14 Yale University Transient transfection with rna
WO2009032456A2 (en) * 2007-08-01 2009-03-12 Primegen Biotech Llc Non-viral delivery of transcription factors that reprogram human somatic cells into a stem cell-like state
WO2009062157A1 (en) * 2007-11-09 2009-05-14 Michigan State University Epigenetic modification of cell phenotype, fate and/or function by rna transfer
EP2072618A1 (en) * 2007-12-14 2009-06-24 Johannes Gutenberg-Universität Mainz Use of RNA for reprogramming somatic cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070087437A1 (en) * 2005-10-14 2007-04-19 Jifan Hu Methods for rejuvenating cells in vitro and in vivo
WO2008097926A2 (en) * 2007-02-02 2008-08-14 Yale University Transient transfection with rna
WO2009032456A2 (en) * 2007-08-01 2009-03-12 Primegen Biotech Llc Non-viral delivery of transcription factors that reprogram human somatic cells into a stem cell-like state
WO2009062157A1 (en) * 2007-11-09 2009-05-14 Michigan State University Epigenetic modification of cell phenotype, fate and/or function by rna transfer
EP2072618A1 (en) * 2007-12-14 2009-06-24 Johannes Gutenberg-Universität Mainz Use of RNA for reprogramming somatic cells

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
OKITA KEISUKE ET AL: "Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, WASHINGTON, DC, vol. 322, no. 5903, 7 November 2008 (2008-11-07), pages 949 - 953, XP002531346, ISSN: 0036-8075 *
PARK IN-HYUN ET AL: "Reprogramming of human somatic cells to pluripotency with defined factors", NATURE, NATURE PUBLISHING GROUP, LONDON, UK, vol. 451, no. 7175, 23 December 2007 (2007-12-23), pages 141 - 147, XP002475655, ISSN: 0028-0836 *
PONSAERTS P ET AL: "Messenger RNA electroporation is highly efficient in mouse embryonic stem cells: successful FLPe- and Cre-mediated recombination", GENE THERAPY, MACMILLAN PRESS LTD., BASINGSTOKE, GB, vol. 11, no. 21, 1 November 2004 (2004-11-01), pages 1606 - 1610, XP002483809, ISSN: 0969-7128 *
PONSAERTS P ET AL: "Modulation of cellular behavior by exogenous messenger RNA.", LEUKEMIA : OFFICIAL JOURNAL OF THE LEUKEMIA SOCIETY OF AMERICA, LEUKEMIA RESEARCH FUND, U.K MAY 2006, vol. 20, no. 5, May 2006 (2006-05-01), pages 767 - 769, XP002544196, ISSN: 0887-6924 *
RABINOVICH PETER M ET AL: "Synthetic messenger RNA as a tool for gene therapy", HUMAN GENE THERAPY, MARY ANN LIEBERT, NEW YORK ,NY, US, vol. 17, no. 10, 1 October 2006 (2006-10-01), pages 1027 - 1035, XP002489890, ISSN: 1043-0342 *
RATAJCZAK J ET AL: "Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery", LEUKEMIA, MACMILLAN PRESS LTD, US, vol. 20, no. 5, 1 May 2006 (2006-05-01), pages 847 - 856, XP002531347, ISSN: 0887-6924 *
SMITS E ET AL: "RNA-based gene transfer for adult stem cells and T cells", LEUKEMIA, MACMILLAN PRESS LTD, US, vol. 18, no. 11, 1 November 2004 (2004-11-01), pages 1898 - 1902, XP002392460, ISSN: 0887-6924 *
STADTFELD MATTHIAS ET AL: "Induced Pluripotent Stem Cells Generated Without Viral Integration", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, WASHINGTON, DC, vol. 322, no. 5903, 7 November 2008 (2008-11-07), pages 945 - 949, XP002531345, ISSN: 0036-8075 *
TAKAHASHI K ET AL: "Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 126, no. 4, 25 August 2006 (2006-08-25), pages 663 - 676, XP003013968, ISSN: 0092-8674 *
WERNIG MARIUS ET AL: "In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state", NATURE, NATURE PUBLISHING GROUP, LONDON, UK, vol. 448, no. 7151, 1 July 2007 (2007-07-01), pages 318 - 325, XP002468993, ISSN: 0028-0836 *
XIONG-ZHI WU: "Origin of Cancer Stem Cells: The Role of Self-Renewal and Differentiation", ANNALS OF SURGICAL ONCOLOGY, SPRINGER-VERLAG, NE, vol. 15, no. 2, 28 November 2007 (2007-11-28), pages 407 - 414, XP019568672, ISSN: 1534-4681 *

Also Published As

Publication number Publication date
WO2009093022A2 (en) 2009-07-30
GB0801215D0 (en) 2008-02-27

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