WO2009079308A3 - Composition and method of use of sheep fab phage display library - Google Patents

Composition and method of use of sheep fab phage display library Download PDF

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Publication number
WO2009079308A3
WO2009079308A3 PCT/US2008/086289 US2008086289W WO2009079308A3 WO 2009079308 A3 WO2009079308 A3 WO 2009079308A3 US 2008086289 W US2008086289 W US 2008086289W WO 2009079308 A3 WO2009079308 A3 WO 2009079308A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
phage display
methods
composition
display library
Prior art date
Application number
PCT/US2008/086289
Other languages
French (fr)
Other versions
WO2009079308A2 (en
Inventor
Keting Chu
Randy Yang
Jianfu Jeffrey Wang
Original Assignee
Digitab Inc
Keting Chu
Randy Yang
Jianfu Jeffrey Wang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Digitab Inc, Keting Chu, Randy Yang, Jianfu Jeffrey Wang filed Critical Digitab Inc
Publication of WO2009079308A2 publication Critical patent/WO2009079308A2/en
Publication of WO2009079308A3 publication Critical patent/WO2009079308A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

Abstract

The present invention discloses methods of producing sheep monoclonal antibodies by expanding mRNA. One method involves the construction of sheep monoclonal Fab antibodies by phage display. The present invention also discloses antibodies produced by the methods disclosed herein. Other methods of the present invention involve using the antibodies produced by the methods of the present invention to treat patients, perform diagnostic procedures, and/or conduct research.
PCT/US2008/086289 2007-12-14 2008-12-10 Composition and method of use of sheep fab phage display library WO2009079308A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1387207P 2007-12-14 2007-12-14
US61/013,872 2007-12-14

Publications (2)

Publication Number Publication Date
WO2009079308A2 WO2009079308A2 (en) 2009-06-25
WO2009079308A3 true WO2009079308A3 (en) 2009-08-13

Family

ID=40796096

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/086289 WO2009079308A2 (en) 2007-12-14 2008-12-10 Composition and method of use of sheep fab phage display library

Country Status (1)

Country Link
WO (1) WO2009079308A2 (en)

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BERGER, S. ET AL.: "'Autoreactive antibodies are present in sheep with Johne's disease and cross-react with Mycobacterium avium subsp. paratuberculosis antigens.'", MICROBES INFECTION., vol. 9, no. 8, 7 April 2007 (2007-04-07), pages 963 - 970, XP022120614, DOI: doi:10.1016/j.micinf.2007.03.016 *
LI, Y. ET AL.: "Sheep monoclonal antibody fragments generated using a phage display system.", JOURNAL OF IMMUNOLOGICAL METHODS., vol. 236, no. 1-2, 6 March 2000 (2000-03-06), pages 133 - 146, XP004190753, DOI: doi:10.1016/S0022-1759(99)00227-6 *
SHAW, I. ET AL.: "Development of a high-affinity anti-domoic acid sheep scFv and its use in detection of the toxin in shellfish.", ANALYTICAL CHEMISTRY, vol. 80, no. 9, 3 April 2008 (2008-04-03), pages 3205 - 3212 *
WHITE, G. P. ET AL.: "A single-chain variable region immunoglobulin library from the abomasal lymph node of sheep infected with the gastrointestinal nematode parasite Haemonchus contortus.", VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY., vol. 78, 2001, pages 117 - 129 *

Also Published As

Publication number Publication date
WO2009079308A2 (en) 2009-06-25

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