WO2009073821A3 - Biopolymer-nucleic acid conjugation - Google Patents

Biopolymer-nucleic acid conjugation Download PDF

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Publication number
WO2009073821A3
WO2009073821A3 PCT/US2008/085596 US2008085596W WO2009073821A3 WO 2009073821 A3 WO2009073821 A3 WO 2009073821A3 US 2008085596 W US2008085596 W US 2008085596W WO 2009073821 A3 WO2009073821 A3 WO 2009073821A3
Authority
WO
WIPO (PCT)
Prior art keywords
biopolymer
nucleic acid
acid conjugation
conjugation
oligonucleotide
Prior art date
Application number
PCT/US2008/085596
Other languages
French (fr)
Other versions
WO2009073821A2 (en
Inventor
Paul Hatala
Ryan Boomer
Markus Kurz
Original Assignee
Archemix Corp.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Archemix Corp. filed Critical Archemix Corp.
Publication of WO2009073821A2 publication Critical patent/WO2009073821A2/en
Publication of WO2009073821A3 publication Critical patent/WO2009073821A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

Abstract

A method for conjugation of biopolymer e.g., polyalkylene glycol (PAG) to an oligonucleotide comprising combining an activated-biopolymer and an oligonucleotide in an aqueous solution at a pH at or greater than 8.5 and adding DMSO to avoid PEG side reactions and degradation.
PCT/US2008/085596 2007-12-04 2008-12-04 Biopolymer-nucleic acid conjugation WO2009073821A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US538207P 2007-12-04 2007-12-04
US61/005,382 2007-12-04

Publications (2)

Publication Number Publication Date
WO2009073821A2 WO2009073821A2 (en) 2009-06-11
WO2009073821A3 true WO2009073821A3 (en) 2009-08-27

Family

ID=40718503

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2008/085594 WO2009073820A2 (en) 2007-12-04 2008-12-04 Biopolymer-nucleic acid conjugation
PCT/US2008/085596 WO2009073821A2 (en) 2007-12-04 2008-12-04 Biopolymer-nucleic acid conjugation

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2008/085594 WO2009073820A2 (en) 2007-12-04 2008-12-04 Biopolymer-nucleic acid conjugation

Country Status (1)

Country Link
WO (2) WO2009073820A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060003326A1 (en) * 2001-10-26 2006-01-05 Christian Lange Modified l-nucleic acid
US20070048248A1 (en) * 2004-02-12 2007-03-01 Claude Benedict Aptamer therapeutics useful in the treatment of complement-related disorders

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002365360A1 (en) * 2001-11-28 2003-06-10 Biopolymed Inc. Biologically active non-antigenic copolymer and conjugates thereof and methods for producing the same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060003326A1 (en) * 2001-10-26 2006-01-05 Christian Lange Modified l-nucleic acid
US20070048248A1 (en) * 2004-02-12 2007-03-01 Claude Benedict Aptamer therapeutics useful in the treatment of complement-related disorders

Also Published As

Publication number Publication date
WO2009073821A2 (en) 2009-06-11
WO2009073820A3 (en) 2009-09-24
WO2009073820A2 (en) 2009-06-11

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