WO2009066075A2 - Diagnostic markers of wound infection - Google Patents
Diagnostic markers of wound infection Download PDFInfo
- Publication number
- WO2009066075A2 WO2009066075A2 PCT/GB2008/003893 GB2008003893W WO2009066075A2 WO 2009066075 A2 WO2009066075 A2 WO 2009066075A2 GB 2008003893 W GB2008003893 W GB 2008003893W WO 2009066075 A2 WO2009066075 A2 WO 2009066075A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- wound
- procalcitonin
- cytokine
- infection
- level
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/575—Hormones
- G01N2333/5753—Calcitonin gene related peptide
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/26—Infectious diseases, e.g. generalised sepsis
Definitions
- the present invention relates to monitoring patients for the onset or development of wound infection, by detecting the presence and/or level of a marker associated with sub-clinical or clinical infection in a wound.
- the marker may be a precursor to a hormone associated with sub-clinical or clinical infection in a wound, especially in wound fluid or maybe a cytokine.
- Wound healing is a complex dynamic process that results in the restoration of anatomic continuity and function; an ideally healed wound is one that has returned to normal anatomic structure, function and appearance.
- a diagnosis of infection is based on the presence of local pain, heat, swelling, discharge and redness, although many clinical indicators, such as inflammation and discharge have a low predictive value of infection in wounds. Diagnosis of infection is commonly confirmed by microbiological analysis of wound samples which may take several days to complete. Delay in diagnosis of infection can delay the administration of antimicrobial therapy and may increase the risk of developing sepsis. Conversely a rapid diagnosis of the absence of infection reduces the inappropriate use of antibiotic therapy.
- cytokines are markers for bacterial inflammation.
- procalcitonin is the peptide precursor of calcitonin. The release of procalcitonin from tissues is induced directly via the presence of bacteria.
- the present inventors have made the surprising discovery that certain cytokines are indicative of wound infection and that the level of cytokine is correlated to the level of infection in the wound. Further the present inventors have discovered that certain cytokines are found in the wound fluid of sub-clinically or clinically infected wounds.
- the present invention relates to markers to predict or confirm patients for wound infection.
- the marker is preferably a cytokine and may be a precursor to a hormone.
- the cytokine may be detected by the use of an assay.
- a first aspect of the present invention provides a method of diagnosis or prediction of infection of a mammalian wound, said method comprising the step of detecting the presence of a cytokine selected from the group comprising procalcitonin, amino procalcitonin (N-ProCT) , eotaxin, granulocyte macrophage colony stimulating factor (GM-CSF) , interleukins IB monocyte chemotactic protein-1 (MCP-I) , macrophage inflammatory protein-1 alpha (MIP-Ia), regulated upon activation normal
- a cytokine selected from the group comprising procalcitonin, amino procalcitonin (N-ProCT) , eotaxin, granulocyte macrophage colony stimulating factor (GM-CSF) , interleukins IB monocyte chemotactic protein-1 (MCP-I) , macrophage inflammatory protein-1 alpha (MIP-Ia), regulated upon activation normal
- RANTES T expressed and secreted
- the present invention provides a method of diagnosis or prediction of infection of a mammalian wound, said method comprising the step of detecting the presence of at least one of the markers selected from the group comprising procalcitonin, amino procalcitonin (N-ProCT) , eotaxin, granulocyte macrophage colony stimulating factor (GM-CSF) , interleukins IB monocyte chemotactic protein-1 (MCP-I) , macrophage inflammatory protein-1 alpha (MIP-Ia) , regulated upon activation normal T expressed and secreted (RANTES) in a sample of wound fluid taken from said wound.
- the markers selected from the group comprising procalcitonin, amino procalcitonin (N-ProCT) , eotaxin, granulocyte macrophage colony stimulating factor (GM-CSF) , interleukins IB monocyte chemotactic protein-1 (MCP-I) , macrophage inflammatory protein-1 alpha (MIP-Ia)
- the finding that at least one of the markers is present in a wound indicates that local infection is evident.
- the marker is detected typically by using an assay that generates a colour change in a test strip.
- the intensity of colour generated is directly proportional to the marker concentration in the sample.
- a diagnostic kit such as BRAHMS PCT-Q may be used to detect the marker.
- procalcitonin a baseline level is less than 0.05ng/ml in a normal healthy subject. After the onset of infection the level of procalcitonin can rise up to 1000 times the initial level within 2 to 3 hours of onset of infection and then continue to increase over the following 24 hours.
- a sample of wound fluid containing procalcitonin in the region of >0.5 and ⁇ 2ng/ml indicates that bacterial infection is highly likely.
- the test strip is accompanied by a chart of reference colours where the intensity of the colour generated on the test strip correlates with the level of marker present.
- the assay has reference bands at ⁇ _0.5ng/ml, 0.5ng/ml, _>_2ng/ml and j>_10ng/ml.
- a sample of wound fluid containing procalcitonin in the region of > 2ng/ml indicates an infected wound and j ⁇ lOng/ml indicates a highly infected wound.
- the test strip will preferably have a positive test band that shows that the test is complete to avoid doubt where no marker is detected.
- Suitable methods include the use of diagnostic equipment such as VIDAS BRAHMS PCT, KRYPTOR and BRAHMS PCT sensitive LIA which use antibodies specific to the marker which bind and produce a fluorescent or luminescent signal proportional to the level of marker present.
- diagnostic equipment such as VIDAS BRAHMS PCT, KRYPTOR and BRAHMS PCT sensitive LIA which use antibodies specific to the marker which bind and produce a fluorescent or luminescent signal proportional to the level of marker present.
- the results of these tests are typically analysed by diagnostic software.
- the analysis of a sample from the wound is carried out by taking a sample of the patients' wound fluid. The fluid is centrifuged and then applied to the test and measured either semi- quantitatively or quantitatively using a rapid kit or laboratory based equipment.
- the presence and severity of the infection can be estimated and the course of treatment decided based on this.
- the success of treatments can also be monitored by repeating the test for example every 12 hours.
- wound fluid refers to any wound exudate or other fluid (substantially not including blood) that is present at the surface of the wound or that is removed from the wound surface by aspiration, absorption or washing.
- wound fluid does not refer to blood or tissue plasma remote from the wound site.
- the invention comprises a diagnostic device for use in diagnosis or prediction of infection of a mammalian wound by measuring the level of a cytokine selected from the group comprising procalcitonin, amino procalcitonin (N-ProCT) , eotaxin, granulocyte macrophage colony stimulating factor (GM-CSF) , interleukins IB monocyte chemotactic protein-1 (MCP-I) , macrophage inflammatory protein- 1 alpha (MIP-Ia) , regulated upon activation normal T expressed and secreted (RANTES) by measuring the presence and level of the cytokine in the wound fluid, wherein the device comprises a binding partner for the cytokine.
- a cytokine selected from the group comprising procalcitonin, amino procalcitonin (N-ProCT) , eotaxin, granulocyte macrophage colony stimulating factor (GM-CSF) , interleukins IB monocyte chemotactic protein-1 (MCP-
- the devices of the present invention can be in the form of a wound dressing comprising the binding partner for the cytokine which on absorption of fluid from the wound form a complex to give a colour change indicative of the presence and level of the cytokine in the wound fluid.
- the devices of the present invention can be in the form of a test kit comprising a pipette, a test strip and a colour scale or a dip stick for dipping in the wound and a colour scale.
- the device is used to determine the presence and level of cytokine present in the wound fluid. According to the level detected a suitable diagnosis can be made and an appropriate treatment given.
- a sample of wound fluid was taken from a wound located on the lower hind limb of a horse.
- the wound fluid was tested using BRAHMS PCT Q kit which comprises a pipette, a lateral flow test strip and a colour scale and can indicate the presence and level of procalcitonin in the wound fluid.
- the test consisted of the application of 200 ⁇ l of wound fluid onto the test area of the strip.
- the formation of an antibody-procalcitonin complex sandwich on the test strip generated a visible red band on the strip, the intensity of which was used to determine the level of procalcitonin using a semi-quantitative colour scale.
- the band indicated that the wound fluid contained procalcitonin in the region of >0.5 and ⁇ 2ng/ml.
- the level of procalcitonin indicated that bacterial infection was highly likely and therefore local and systemic antimicrobial therapy was administered.
- Sterile Saline was prepared at a concentration of 0.9% with distilled water and autoclaved at 121oC.
- Normal Equine Serum Sterile equine serum was obtained from Sigma- Aldrich Ltd (Poole, Dorset) . Lot: 26H4612; Expiry date: 07/2013. The serum was aliquoted, aseptically and frozen at -20 oC until required. Methods Wound exudate collection: Wound exudates were collected during dressing changes and centrifuged for 30 minutes at 2000 rpm in order to remove any blood cells. One set of wound exudates were evaluated immediately. The second set of wound exudates were collected and immediately frozen at -20oC. Frozen samples were thawed for 10 minutes at 37oC and gently vortexed for 30 seconds before applying to the PCT kit.
- PCT analysis was carried out using the BRAHMS PCT-Q kit. This is a semi-quantitative test and takes approximately 30 minutes to obtain a result. Its reading range is between 0.5 ng/ml and 10 ng/ml, and it can be performed in the laboratory or clinic. Six drops of wound exudate were pipetted into the concave cavity of the kit ( ⁇ 200 ⁇ l) and incubated at room temperature for 30 minutes. After which, the PCT concentration was determined by comparing the colour intensity of the test band with the colour blocks of the reference card supplied with the kit. If no control band appears on the card the test is considered to be invalid.
- Table 1 Fresh wound exudate PCT concentrations in eleven horses with chronic or acute wounds were evaluated (Table 1) . Table 1 also includes information on the breed, age, sex, type of wound and signs of infection. The centrifuged exudate was tested immediately after being removed from the wound.
- Wound fluid samples were collected from human chronic wounds and assessed for evidence of procalcitonin using the PCT-Q kit (BRAHMS, Germany) . In most cases, wound fluid samples were diluted (1 :5 or 1 : 10) because the samples were too viscous to measure using the PCT-Q lateral flow system. In one patient (016) , a leg ulcer of 3 years duration was considered to be clinically infected. There was also evidence of biofilm and exudate level, was heavy. Procalcitonin detection using the PCT-Q kit indicated a level of > 0.5 ng/ml. A second patient (018) had a leg ulcer of 4 years duration, with heavy exudate level and evidence of biofilm. Procalcitonin detection using the PCT-Q kit indicated a level of 0.5 ng/ml.
- a third patient (019) had a pressure ulcer of 5 years duration, with heavy exudate level and evidence of biofilm.
- Procalcitonin detection using the PCT-Q kit was negative (i.e. ⁇ 0.5ng/ml) .
- a negative control sample using horse serum produced a negative result in the PCT-Q kit (i.e. ⁇ 0.5nh/ml) .
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NZ585858A NZ585858A (en) | 2007-11-20 | 2008-11-20 | Diagnostic markers of wound infection comprising measurement of procalcitonin or N-ProCT |
US12/743,633 US10739352B2 (en) | 2007-11-20 | 2008-11-20 | Diagnosis and treatment of wound infection with procalcitonin as diagnostic marker |
EP08852268A EP2225564A2 (en) | 2007-11-20 | 2008-11-20 | Diagnostic markers of wound infection |
JP2010534539A JP2011503631A (en) | 2007-11-20 | 2008-11-20 | Diagnostic marker for wound infection |
AU2008327712A AU2008327712B2 (en) | 2007-11-20 | 2008-11-20 | Diagnostic markers of wound infection |
CA2706080A CA2706080C (en) | 2007-11-20 | 2008-11-20 | Diagnostic markers of wound infection |
MX2010005577A MX2010005577A (en) | 2007-11-20 | 2008-11-20 | Diagnostic markers of wound infection. |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0722729.1A GB0722729D0 (en) | 2007-11-20 | 2007-11-20 | Diagnostic markers of wound infection |
GB0722729.1 | 2007-11-20 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009066075A2 true WO2009066075A2 (en) | 2009-05-28 |
WO2009066075A3 WO2009066075A3 (en) | 2009-09-03 |
Family
ID=38896592
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB2008/003893 WO2009066075A2 (en) | 2007-11-20 | 2008-11-20 | Diagnostic markers of wound infection |
Country Status (9)
Country | Link |
---|---|
US (1) | US10739352B2 (en) |
EP (1) | EP2225564A2 (en) |
JP (1) | JP2011503631A (en) |
AU (1) | AU2008327712B2 (en) |
CA (1) | CA2706080C (en) |
GB (1) | GB0722729D0 (en) |
MX (1) | MX2010005577A (en) |
NZ (1) | NZ585858A (en) |
WO (1) | WO2009066075A2 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102081101A (en) * | 2009-10-13 | 2011-06-01 | B.R.A.H.M.S有限公司 | Procalcitonin for the diagnosis of bacterial infections and guidance of antibiotic treatment in patients with acute stroke or transient ischemic attack |
CN102667486A (en) * | 2009-11-25 | 2012-09-12 | 霍洛吉克股份有限公司 | Detection of intraamniotic infection |
CN104122401A (en) * | 2014-07-24 | 2014-10-29 | 深圳职业技术学院 | Test paper for procalcitonin and C reactive protein as well as preparation method and detection method thereof |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP4368156A2 (en) | 2017-11-09 | 2024-05-15 | ConvaTec Technologies Inc. | Ostomy monitoring system and method |
USD893514S1 (en) | 2018-11-08 | 2020-08-18 | 11 Health And Technologies Limited | Display screen or portion thereof with graphical user interface |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004086043A1 (en) * | 2003-03-26 | 2004-10-07 | Johnson & Johnson Medical Limited | Prediction and detection of wound infection |
GB2430031A (en) * | 2005-09-07 | 2007-03-14 | Ethicon Inc | Diagnostic markers of wound infection |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2430031A1 (en) * | 1974-06-22 | 1976-01-15 | Walter Dr Schade | Mounting for reciprocating church bells - roller carriage to accommodate horizontal forces by further roller mass |
DE19600875C1 (en) | 1996-01-12 | 1997-06-26 | Brahms Diagnostica Gmbh | Diagnostic procedure for determining the etiology of inflammatory processes |
GB2340235A (en) | 1998-08-05 | 2000-02-16 | Johnson & Johnson Medical Ltd | Monitoring bacterial contamination of a wound involving assay of adenosine triphosphate |
CA2370509A1 (en) | 1999-04-26 | 2000-11-02 | The Procter & Gamble Company | A blood detection composition |
US6426227B1 (en) | 1999-08-31 | 2002-07-30 | Common Sense Ltd. | Method for analyzing secreted bodily fluids |
EP1110970B1 (en) * | 1999-12-22 | 2008-05-14 | Dade Behring Marburg GmbH | Anti-Procalcitonin antibodies, their production and use |
KR100348351B1 (en) | 2000-05-24 | 2002-08-09 | 주식회사 바이오디지트 | Electrochemical membrane strip biosensor |
US7465555B2 (en) * | 2002-04-02 | 2008-12-16 | Becton, Dickinson And Company | Early detection of sepsis |
DE102004041659A1 (en) * | 2004-08-27 | 2006-03-02 | Institut Virion/Serion Gmbh | Test device for the in vitro diagnosis of multi-analyte tests and their use |
-
2007
- 2007-11-20 GB GBGB0722729.1A patent/GB0722729D0/en not_active Ceased
-
2008
- 2008-11-20 EP EP08852268A patent/EP2225564A2/en not_active Withdrawn
- 2008-11-20 MX MX2010005577A patent/MX2010005577A/en active IP Right Grant
- 2008-11-20 CA CA2706080A patent/CA2706080C/en active Active
- 2008-11-20 JP JP2010534539A patent/JP2011503631A/en active Pending
- 2008-11-20 US US12/743,633 patent/US10739352B2/en active Active
- 2008-11-20 AU AU2008327712A patent/AU2008327712B2/en active Active
- 2008-11-20 NZ NZ585858A patent/NZ585858A/en not_active IP Right Cessation
- 2008-11-20 WO PCT/GB2008/003893 patent/WO2009066075A2/en active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004086043A1 (en) * | 2003-03-26 | 2004-10-07 | Johnson & Johnson Medical Limited | Prediction and detection of wound infection |
GB2430031A (en) * | 2005-09-07 | 2007-03-14 | Ethicon Inc | Diagnostic markers of wound infection |
Non-Patent Citations (7)
Title |
---|
DATABASE CA [Online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; JIANG, QIAN ET AL: "The detection and significance of serum eotaxin in children with respiratory adenovirus infection" XP002528856 retrieved from STN Database accession no. 145:311772 & ZHONGGUO QUANKE YIXUE , 8(10), 802-803 CODEN: ZQYHAK; ISSN: 1007-9572, 2005, * |
ELLMARK, PETER ET AL: "Identification of protein expression signatures associated with Helicobacter pylori infection and gastric adenocarcinoma using recombinant antibody microarrays" MOLECULAR AND CELLULAR PROTEOMICS , 5(9), 1638-1646 CODEN: MCPOBS; ISSN: 1535-9476, 2006, XP002528853 * |
FIVENSON DAVID P ET AL: "Chemokine and inflammatory cytokine changes during chronic wound healing" WOUND REPAIR AND REGENERATION, vol. 5, no. 4, October 1997 (1997-10), pages 310-322, XP002509882 ISSN: 1067-1927 * |
FORSBERG JA AND ELSTER E: "Cytokine expression correlates with wound dehiscence in wartime extremity injuries" JOURNAL OF SURGICAL RESEARCH, vol. 137, no. 2, 25 January 2007 (2007-01-25), pages 208-209, XP002528854 * |
FORSBERG JONATHAN AGNER ET AL: "Correlation of procalcitonin and cytokine expression with dehiscence of wartime extremity wounds" JOURNAL OF BONE AND JOINT SURGERY, JOURNAL OF BONE AND JOINT SURGERY. BOSTON, US, vol. 90, no. 3, 1 March 2008 (2008-03-01), pages 580-588, XP009110613 ISSN: 0021-9355 * |
PAPADOPOULOS N G ET AL: "Rhinovirus infection up-regulates eotaxin and eotaxin-2 expression in bronchial epithelial cells" CLINICAL AND EXPERIMENTAL ALLERGY, vol. 31, no. 7, July 2001 (2001-07), pages 1060-1066, XP002528855 ISSN: 0954-7894 * |
SCHNEIDER HANS-GERHARD ET AL: "Procalcitonin for the clinical laboratory: a review" PATHOLOGY, vol. 39, no. 4, August 2007 (2007-08), pages 383-390, XP002509883 ISSN: 0031-3025 * |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102081101A (en) * | 2009-10-13 | 2011-06-01 | B.R.A.H.M.S有限公司 | Procalcitonin for the diagnosis of bacterial infections and guidance of antibiotic treatment in patients with acute stroke or transient ischemic attack |
CN102667486A (en) * | 2009-11-25 | 2012-09-12 | 霍洛吉克股份有限公司 | Detection of intraamniotic infection |
CN102667486B (en) * | 2009-11-25 | 2016-03-09 | 霍洛吉克股份有限公司 | The detection of IAI |
US10215760B2 (en) | 2009-11-25 | 2019-02-26 | Hologic, Inc. | Detection of intraamniotic and/or infection |
CN104122401A (en) * | 2014-07-24 | 2014-10-29 | 深圳职业技术学院 | Test paper for procalcitonin and C reactive protein as well as preparation method and detection method thereof |
Also Published As
Publication number | Publication date |
---|---|
AU2008327712A1 (en) | 2009-05-28 |
AU2008327712B2 (en) | 2014-11-06 |
US20110039342A1 (en) | 2011-02-17 |
WO2009066075A3 (en) | 2009-09-03 |
US10739352B2 (en) | 2020-08-11 |
NZ585858A (en) | 2012-10-26 |
GB0722729D0 (en) | 2007-12-27 |
JP2011503631A (en) | 2011-01-27 |
MX2010005577A (en) | 2010-06-02 |
CA2706080C (en) | 2020-07-07 |
EP2225564A2 (en) | 2010-09-08 |
CA2706080A1 (en) | 2009-05-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US8012698B2 (en) | Diagnostic markers of wound infection | |
Burgess et al. | Expression of connective tissue growth factor in asthmatic airway smooth muscle cells | |
CA2706080C (en) | Diagnostic markers of wound infection | |
Trøstrup et al. | S100A8/A9 is an important host defence mediator in neuropathic foot ulcers in patients with type 2 diabetes mellitus | |
GB2381452A (en) | Method for predicting or diagnosing infection in a wound | |
Altay et al. | Does treatment affect the levels of serum interleukin-6, interleukin-8 and procalcitonin in diabetic foot infection? A pilot study | |
Saleh et al. | Inflammation biomarkers and correlation to wound status after full-thickness skin grafting | |
CN109844529B (en) | Method for identifying non-healing skin wounds and for monitoring skin wound healing | |
Cerveró-Ferragut et al. | Quantitative analysis of blood cells and inflammatory factors in wounds | |
Xu et al. | Constitutive release of cytokines by human oral keratinocytes in an organotypic culture | |
JP2020517955A (en) | Method and device for determining the efficacy of statins for the treatment of inflammatory diseases in individual patients | |
WO2004086043A1 (en) | Prediction and detection of wound infection | |
WO2009122188A2 (en) | Wound infection monitoring method | |
Kumar et al. | Dynamic alteration in the vaginal secretory proteome across the early and mid-trimesters of pregnancy | |
RU2744145C1 (en) | Method for prediction of sepsis in severely injured from burn injury | |
CN118068003A (en) | Application of drug intervention target for treating and/or relieving traumatic inflammation | |
GB2430031A (en) | Diagnostic markers of wound infection | |
KR20110128941A (en) | Method of invasiveness control and use of adiponectin | |
KR20240037200A (en) | Scar diagnostic reagents and their applications | |
JP2021117164A (en) | Method of evaluating skin inflammatory conditions | |
CN117288961A (en) | Application of CRISP3 protein in preparation of biomarker for early warning of sepsis | |
WO2018223001A1 (en) | Predictive factors for timing of wound closure | |
Widgerow et al. | Burn wound fluid: an important diagnostic source | |
CN117783533A (en) | Application of blood biological marker as uremia calcification defense disease diagnosis and curative effect detection marker | |
Judy et al. | Wound-Dermatological Management/Issues: 3442: ATROPHIE BLANCHE: SPECIFIC DISEASE OR PHYSICAL FINDING? |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 08852268 Country of ref document: EP Kind code of ref document: A2 |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2706080 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 12743633 Country of ref document: US |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2008327712 Country of ref document: AU Ref document number: 2010534539 Country of ref document: JP Ref document number: MX/A/2010/005577 Country of ref document: MX |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
WWE | Wipo information: entry into national phase |
Ref document number: 585858 Country of ref document: NZ |
|
ENP | Entry into the national phase |
Ref document number: 2008327712 Country of ref document: AU Date of ref document: 20081120 Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2008852268 Country of ref document: EP |