WO2009063476A1 - A crystalline form of atorvastatin hemi magnesium salt and a process thereof - Google Patents
A crystalline form of atorvastatin hemi magnesium salt and a process thereof Download PDFInfo
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- WO2009063476A1 WO2009063476A1 PCT/IN2007/000623 IN2007000623W WO2009063476A1 WO 2009063476 A1 WO2009063476 A1 WO 2009063476A1 IN 2007000623 W IN2007000623 W IN 2007000623W WO 2009063476 A1 WO2009063476 A1 WO 2009063476A1
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D207/00—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D207/02—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
- C07D207/30—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members
- C07D207/34—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
Definitions
- the present invention relates to the preparation of new polymorphs of Atorvastatin hemi Magnesium salt designated as Form B4, B5 and B6 as well as methods of preparation thereof.
- the said compound is useful as inhibitor of the enzyme HMG-CoA reductase and thus useful as a hypolipidemic and hypocholesterolemic agent.
- Atorvastatin is a member of statins.
- Statin drugs are currently the most therapeutically effective drugs available for reducing low density lipoprotein particle concentration in the blood stream of patients at risk for cardiovascular disease.
- a high level of low density lipoprotein in the bloodstream has been linked to the formation of coronary lesions which obstruct the flow of blood and can rupture and promote thrombosis.
- Reducing low density lipoprotein levels has been shown to reduce the risk of clinical events in patients with cardiovascular disease and patients who are free of cardiovascular disease but who have hypercholesterolemia.
- the crystalline and amorphous forms have different properties due to the unique arrangement of molecules in the crystal lattice varying density of packing, and/or by varying hydrogen-bond network. Accordingly, individual crystalline and amorphous forms may be thought of as distinct solids having distinct advantageous and/or disadvantageous and/ or physical properties compared to other polymorphic forms.
- a single molecule, like the atorvastatin or the salt complex may give rise to a variety of solids having distinct physical properties like melting point, X-ray diffraction pattern, IR and NMR spectrum. The differences in the physical properties of polymorphs result from the orientation and intermolecular interactions of adjacent molecules in the bulk solid.
- the present invention relates to crystalline forms B4, B5 and B6 of atorvastatin magnesium, the processes for their preparation and isolation, pharmaceutical compositions which include the forms B4, B5 or B6, and a pharmaceutically acceptable carrier, and to a method of administering a therapeutic amount of the pharmaceutical composition for the treatment of hyperlipidemia and hypercholesterolemia
- the present invention provides for new polymorphic forms of atorvastatin magnesium, i.e. crystalline forms B4, B5 and B6, characterized by a X-ray powder diffraction pattern.
- the present invention provides new processes for preparation of atorvastatin magnesium forms B4, B5 and B6.
- the invention provides pharmaceutical compositions and dosage forms comprising atorvastatin magnesium forms B4 or B5 or B6.
- a still further embodiment of the present invention is a method of treating hyperlipidemia or hypercholesteremia with a pharmaceutical composition containing a therapeutically effective amount of atorvastatin magnesium crystalline forms B4, B5 and B6.
- the principal object of the present invention is to prepare new crystalline forms of atorvastatin hemi magnesium salt.
- Another object of the present invention is to develop a process for the preparation new crystalline forms of atorvastatin hemi magnesium salt.
- Still other object of the present invention is to develop a pharmaceutical composition
- a pharmaceutical composition comprising atorvastatin magnesium form B4, B5 or B6 optionally along with pharmaceutically acceptable excipient.
- the present invention relates to a crystalline form of atorvastatin hemi magnesium salt characterized by X-ray diffraction pattern comprising 2 theta values 3.49, 4.45, 5.32, 5.58, 6.99, 7.96, 8.30, 9.12, 11.96, 19.01 and 19.80 ⁇ 0.2; a crystalline form of atorvastatin hemi magnesium salt characterized by X-ray diffraction pattern comprising 2 theta values 3.45, 5.19, 6.06, 6.87, 7.64, 8.38, 10.29, 1 1.22, 1 1.70, 12.11, 15.22, 18.1 1, 22.30, 22.84, 24.15and 27.73 ⁇ 0.2; a crystalline form of atorvastatin hemi magnesium salt characterized by X-ray diffraction pattern comprising 2 theta values 3.59, 4.62, 5.34, 9.39 and 1 1.0 ⁇ 0.2; a process for preparation of crystalline atorvastatin hemi magnesium salt, wherein said process comprising steps of:
- Fig.l Characteristic powder X-ray powder diffraction pattern of Atorvastatin magnesium Crystalline form B4.
- Fig. 2 Characteristic powder diffraction pattern of Atorvastatin magnesium crystalline form B5.
- Fig. 3 Characteristic powder diffraction pattern of Atorvastatin magnesium crystalline form B6.
- the present invention is in relation to a crystalline form of atorvastatin hemi magnesium salt characterized by X-ray diffraction pattern comprising 2 theta values 3.49, 4.45, 5.32, 5.58, 6.99, 7.96, 8.30, 9.12, 11.96, 19.01 and 19.80 ⁇ 0.2
- the present invention is in relation to a crystalline form of atorvastatin hemi magnesium salt characterized by X-ray diffraction pattern comprising 2 theta values
- the present invention is in relation to a crystalline form of atorvastatin hemi magnesium salt characterized by X-ray diffraction pattern comprising 2 theta values
- the crystalline form is B5. In still another embodiment of the present invention wherein the crystalline form is B6.
- the melting point is in the range of 147°C to 168°C.
- the present invention is in relation to a process for preparation of crystalline atorvastatin hemi magnesium salt, wherein said process comprising steps of: treating amorphous Atorvastatin hemi magnesium salt in water or a mixture of organic solvents and water; optionally heating the mixture with stirring followed by isolating the crystalline form of amorphous atorvastatin hemi magnesium salt by filtration; and drying to obtain crystalline atorvastatin hemi magnesium salt.
- the solvent used is selected from a group comprising water or a mixture of organic solvents and water.
- the organic solvent is selected from a group comprising hydroxylic, non-hydroxylic solvent or mixture thereof.
- the organic solvent is selected from a group comprising acetone, methanol, ethanol, ethyl acetate, isopropyl alcohol, THF, dichloromethane, t-butanol, iso-butanol, carbon tetrachloride, 1,4- dioxan, n-butanol, di-isopropyl ether, di-ethyl ether and mixtures thereof.
- the mixture is heated to a temperature of about 40 0 C.
- the present invention is in relation to a crystalline atorvastatin hemi magnesium salt as claimed in any of the preceding claims having a particle size ranging from 1 micron to 150 microns.
- the present invention is in relation to a pharmaceutical composition
- a pharmaceutical composition comprising crystalline atorvastatin magnesium form selected from a group comprising B4, B5, B6 and combinations thereof optionally along with pharmaceutically acceptable excipients.
- the excipients are selected from a group comprising granulating agents, binding agents, lubricating agents, disintegrating agents, sweetening agents, coloring agents, flavoring agents, coating agents, plasticizers, preservatives, suspending agents, emulsifying agents and spheronization agents.
- composition is formulated into dosage forms selected from a group comprising tablet, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsion in hard or soft gel capsules, syrups, elixirs, phyotceuticals, neutraceuticals and food stuffs.
- the present invention provides for new crystalline forms of atorvastatin hemi magnesium salt B4, B5 and B6 characterized by a X-ray powder diffraction pattern as depicted in Fig 1, 2 & 3 respectively.
- the present invention provides new processes for the preparation of atorvastatin hemi magnesium salt forms B4, B5 and B6, According to yet another aspect the invention provides pharmaceutical compositions and dosage forms comprising atorvastatin magnesium forms B4, B5 or B6.
- a still further embodiment of the present invention relates to a method of treating hyperlipidemia or hypercholesteremia with a pharmaceutical composition containing a therapeutically effective amount of atorvastatin magnesium crystalline forms B4, B5 and B6.
- Particle size of the crystalline forms atorvastatin hemi magnesium salt is about from 1 to 150 microns.
- the present invention provides polymorphic forms of atorvastatin hemi magnesium salt, the present invention provides atorvastatin hemi magnesium salt in two new polymorphic forms denominated as crystalline forms" B4", " B5", and "B6".
- This invention is related to crystalline forms B4, B5 and B6 of atorvastatin hemi magnesium salt having the following chemical structure:
- the present invention is further directed to the processes for the production and isolation of forms of B4 , B5 or B6, to pharmaceutical compositions which include the crystalline forms B4 or B5, and a pharmaceutically acceptable carrier, and to a method of administering a therapeutic amount of the pharmaceutical composition for the treatment of hyperlipidemia and hypercholesterolemia.
- the B4, B5 and B6 forms of atorvastatin magnesium are useful as inhibitors of the enzyme HMG-CoA reductase, and therefore, are useful for treating hyperlipidemia and hypercholesterolemia.
- the B4, B5 and B6 forms are characterized by their distinctive X-ray powder diffractograms as depicted in Fig 1, 2 & 3 respectively.
- the present invention also provides for a method for the preparation of crystalline forms B4 and B5 of atorvastatin hemi magnesium salt.
- the method comprises exposing atorvastatin to different solvents and temperature conditions, which yield crystalline forms B4, B5 and B6.
- Crystalline atorvastatin hemi magnesium salt form B4, B5 and B6 may be prepared under proper conditions. In particular, they can be prepared/ isolated by crystallization from aqueous, water-miscible, non- aqueous or non-polar solvents at a suitable temperature. Suitable solvents comprise water, acetone, methanol, ethanol, ethyl acetate, isopropyl alcohol, THF, dichloromethane, t-butanol, iso-butanol, carbon tetrachloride, 1,4-dioxan, n-butanol, di-isopropyl ether or di-ethyl ether.
- amorphous form of atorvastatin hemi magnesium salt is treated with water or a mixture of two or more suitable solvents or mixture of a solvent and water under a suitable temperature range and the mixture can be then filtered and dried, preferably under vacuum, to obtain crystalline atorvastatin hemi magnesium salt.
- the used solvents can be removed by various drying methods such as vacuum drying, rapid solvent evaporation , spray drying, freeze drying, solvent precipitation, roller drying and the like.
- the present invention contemplates temperature variations during heating the mixture. Heating may be carried out at temperatures ranging from 4O 0 C to 100 0 C. According to a preferred embodiment, the heating temperature preferably ranges from 40 0 C to 70 0 C.
- Amorphous form of atorvastatin hemi magnesium salt can be prepared from ATV-I boronate (formula II) by following the methods disclosed in WO 2007/063551.
- the polymorph B4 may be obtained by stirring amorphous Atorvastatin hemi magnesium salt in a mixture of water and acetone followed by the separation of solids and drying. In the same manner stirring amorphous Atorvastatin hemi magnesium salt in water at reflection will produce crystalline form B5.
- the method of making crystalline Atorvastatin hemi magnesium salt having at least one of the defining characteristics exemplified in the foregoing description include steps of: treating amorphous atorvastatin hemi magnesium salt in water or a mixture of organic solvents and water, optionally heating the mixture with stirring, isolating the crystalline form of amorphous atorvastatin hemi magnesium salt by filtration and subsequent drying.
- the polymorphs exemplified in invention may be formulated in the form of tablets, pills, powder mixtures, capsules, injectables, solutions, suspensions, suppositories, emulsions, dispersions, food premix, and in other suitable forms. It may also be manufactured in the form of sterile solid compositions, for example, freeze dried and, if desired, combined with other pharmaceutically acceptable excipients, carriers, diluents or adjuvants. According to a preferred aspect of the invention the particle size of crystalline atorvastatin hemi magnesium salt is about from 1 to 150 microns.
- Example-1 preparation of amorphous atorvastatin hemi magnesium salt (disclosed in WO 2007/063551)
- Amorphous atorvastatin hemi magnesium salt was obtained by adding ATV-I boronate (formula II) to a mixture of water and methanol followed by the hydrolysis in the presence of NaOH solution at reflux temperature for about 4 h. The mass was cooled and washed with methyl-tert-butyl ether. The aqueous layer was subjected to vacuum at about 30 0 C to obtain the solids and stirred at room temperature. The sodium salt obtained was filtered and dissolved in a mixture of ethyl acetate and water. The pH of the mass was adjusted to 8-8.5 using HCl solutions followed by the addition of magnesium acetate. The mass was heated to 40-45° C under stirring.
- the mass was cooled to room temperature and the organic layer was separated.
- the organic layer was washed with water and concentrated to syrup under vacuum followed by stripping off with methanol and concentrated to syrup.
- the syrup obtained was dried under vacuum at 40-50° C to obtain amorphous Atorvastatin hemi magnesium salt.
- Amorphous atorvastatin hemi magnesium salt (2 g) was suspended in a mixture of acetone and water (1:1 mixture, 30 mL) and the resulting mass was stirred at room temperature for 65 h.
- the solids were filtered and dried under vacuum at 50-60° C for 12 h to obtained crystalline atorvastatin hemi magnesium salt (1.5 g).
- the crystals were characterized by a X-Ray diffraction pattern as depicted in Fig.l defining Form B4 having melting point of 155 °C.
- Amorphous atorvastatin hemi magnesium salt (2 g) was suspended in water (30 mL) and resulting mass was stirred at reflux temperature for 48 h. The solids were filtered and dried under vacuum at 50-60° C for 12 h to obtain crystalline atorvastatin hemi magnesium salt (1.4 g). The crystals were characterized by a X-Ray diffraction pattern as depicted in Fig.2 defining Form B5 having melting point of 159 °C.
- Atorvastatin magnesium amorphous (2 g, obtained above) was suspended in a mixture of ethanol and water (1 : 1 mixture, 30 mL) and resulting mass was stirred at 50-60° C for about 72 h. The solids were filtered and dried under vacuum at 50-60° C for 12 h to obtain Atorvastatrn magnesium crystalline (1.2 g). The crystals were characterized by a X-Ray diffraction pattern as depicted in Fig.l defining Form B4 having melting point of 155 0 C.
- Example-5 Example-5:
- Atorvastatin magnesium amorphous (2 g, obtained above) was suspended in a mixture of methanol and water (1 :1 mixture, 40 mL) and resulting mass was stirred at 50-60° C for about 72 h. The solids were filtered and dried under vacuum at 50-60° C for 12 h to obtain Atorvastatin magnesium crystalline (1.4 g). The crystals were characterized by a X-Ray diffraction pattern as depicted in Fig.3 defining Form B6 having melting point of 147 °C.
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Abstract
The present invention provides crystalline forms B4 and B5 of atorvastatin hemi magnesium salt. In addition, it also provides a process for preparation of crystalline forms B4 and B5 of atorvastatin hemi magnesium salt.
Description
A CRYSTALLINE FORM OF ATORVASTATEV HEMI MAGNESIUM SALT
AND A PROCESS THEREOF
FIELD OF THE INVENTION The present invention relates to the preparation of new polymorphs of Atorvastatin hemi Magnesium salt designated as Form B4, B5 and B6 as well as methods of preparation thereof. The said compound is useful as inhibitor of the enzyme HMG-CoA reductase and thus useful as a hypolipidemic and hypocholesterolemic agent.
BACKGROUND AND PRIOR ART OF THE INVENTION
Atorvastatin is a member of statins. Statin drugs are currently the most therapeutically effective drugs available for reducing low density lipoprotein particle concentration in the blood stream of patients at risk for cardiovascular disease. A high level of low density lipoprotein in the bloodstream has been linked to the formation of coronary lesions which obstruct the flow of blood and can rupture and promote thrombosis. Reducing low density lipoprotein levels has been shown to reduce the risk of clinical events in patients with cardiovascular disease and patients who are free of cardiovascular disease but who have hypercholesterolemia.
The crystalline and amorphous forms have different properties due to the unique arrangement of molecules in the crystal lattice varying density of packing, and/or by varying hydrogen-bond network. Accordingly, individual crystalline and amorphous forms may be thought of as distinct solids having distinct advantageous and/or disadvantageous and/ or physical properties compared to other polymorphic forms. A single molecule, like the atorvastatin or the salt complex may give rise to a variety of solids having distinct physical properties like melting point, X-ray diffraction pattern, IR and NMR spectrum. The differences in the physical properties of polymorphs result from the orientation and intermolecular interactions of adjacent molecules in the bulk solid. One of the most important physical properties of pharmaceutical polymorphs is their solubility in aqueous solution, particularly their solubility in the gastric juices of a patient. On the other hand, where the effectiveness of a drug correlates with peak bloodstream levels of the drug, a more rapidly dissolving form is likely to exhibit increased effectiveness over a comparable amount of a more slowly dissolving form.
The present invention relates to crystalline forms B4, B5 and B6 of atorvastatin magnesium, the processes for their preparation and isolation, pharmaceutical compositions which include the forms B4, B5 or B6, and a pharmaceutically acceptable carrier, and to a method of administering a therapeutic amount of the pharmaceutical composition for the treatment of hyperlipidemia and hypercholesterolemia
The present invention provides for new polymorphic forms of atorvastatin magnesium, i.e. crystalline forms B4, B5 and B6, characterized by a X-ray powder diffraction pattern.
In another aspect, the present invention provides new processes for preparation of atorvastatin magnesium forms B4, B5 and B6. In another aspect, the invention provides pharmaceutical compositions and dosage forms comprising atorvastatin magnesium forms B4 or B5 or B6.
A still further embodiment of the present invention is a method of treating hyperlipidemia or hypercholesteremia with a pharmaceutical composition containing a therapeutically effective amount of atorvastatin magnesium crystalline forms B4, B5 and B6.
Many patents are disclosed atorvastatin, formulation of atorvastatin and polymorphs as well as processes and key intermediates. These include US 4,681,893; US 5,273,995; US 5,003,080; US 5,097,045; US 5,103,024; US 5,124,482; US 5,149,837; US 5,155,251; US 5,969,156; US 6,087,511; US 6,121,461 which are herein incorporated by reference.
Additionally, a number of published International Patent Applications have disclosed crystalline forms of atorvastatin, as well as processes for preparing amorphous atorvastatin and polymorphs of atorvastatin magnesium WO 2006/021969 and WO 2006/1 17761 ; WO 2007/057755; WO 2007/099552; WO 2007/1 18873.
Preparation of amorphous Atorvastatin hemi magnesium salt is described in detail in WO 2007/063551, the content of which is incorporated herein by reference in its entirety.
OBJECTS OF THE PRESENT INVENTION
The principal object of the present invention is to prepare new crystalline forms of atorvastatin hemi magnesium salt.
Another object of the present invention is to develop a process for the preparation new crystalline forms of atorvastatin hemi magnesium salt.
Still other object of the present invention is to develop a pharmaceutical composition comprising atorvastatin magnesium form B4, B5 or B6 optionally along with pharmaceutically acceptable excipient.
STATEMENT OF THE INVENTION
Accordingly the present invention relates to a crystalline form of atorvastatin hemi magnesium salt characterized by X-ray diffraction pattern comprising 2 theta values 3.49, 4.45, 5.32, 5.58, 6.99, 7.96, 8.30, 9.12, 11.96, 19.01 and 19.80 ± 0.2; a crystalline form of atorvastatin hemi magnesium salt characterized by X-ray diffraction pattern comprising 2 theta values 3.45, 5.19, 6.06, 6.87, 7.64, 8.38, 10.29, 1 1.22, 1 1.70, 12.11, 15.22, 18.1 1, 22.30, 22.84, 24.15and 27.73± 0.2; a crystalline form of atorvastatin hemi magnesium salt characterized by X-ray diffraction pattern comprising 2 theta values 3.59, 4.62, 5.34, 9.39 and 1 1.0 ± 0.2; a process for preparation of crystalline atorvastatin hemi magnesium salt, wherein said process comprising steps of: treating amorphous Atorvastatin hemi magnesium salt in water or a mixture of organic solvents and water, optionally heating the mixture with stirring followed by isolating the crystalline form of amorphous atorvastatin hemi magnesium salt by filtration; and drying to obtain crystalline atorvastatin hemi magnesium salt; a crystalline atorvastatin hemi magnesium salt as claimed in any of the preceding claims having a particle size ranging from 1 micron to 150 microns; and a pharmaceutical composition comprising crystalline atorvastatin magnesium form selected from a group comprising B4, B5, B6 and combinations thereof optionally along with pharmaceutically acceptable excipients.
BRIEF DESCRIPTION OF THE ACCOMPANYING DRAWINGS
Fig.l: Characteristic powder X-ray powder diffraction pattern of Atorvastatin magnesium
Crystalline form B4.
Fig. 2: Characteristic powder diffraction pattern of Atorvastatin magnesium crystalline form B5.
Fig. 3: Characteristic powder diffraction pattern of Atorvastatin magnesium crystalline form B6.
DETAILED DESCRIPTION OF THE INVENTION
The present invention is in relation to a crystalline form of atorvastatin hemi magnesium salt characterized by X-ray diffraction pattern comprising 2 theta values 3.49, 4.45, 5.32, 5.58, 6.99, 7.96, 8.30, 9.12, 11.96, 19.01 and 19.80 ± 0.2
The present invention is in relation to a crystalline form of atorvastatin hemi magnesium salt characterized by X-ray diffraction pattern comprising 2 theta values
3.45, 5.19, 6.06, 6.87, 7.64, 8.38, 10.29, 11.22, 11.70, 12.1 1, 15.22, 18.1 1, 22.30,
22.84, 24.15and 27.73± 0.2 The present invention is in relation to a crystalline form of atorvastatin hemi magnesium salt characterized by X-ray diffraction pattern comprising 2 theta values
3.59, 4.62, 5.34, 9.39 and 11.0 ± 0.2.
In another embodiment of the present invention wherein the crystalline form is B4.
In yet another embodiment of the present invention wherein the crystalline form is B5. In still another embodiment of the present invention wherein the crystalline form is B6.
In still another embodiment of the present invention wherein the melting point is in the range of 147°C to 168°C.
The present invention is in relation to a process for preparation of crystalline atorvastatin hemi magnesium salt, wherein said process comprising steps of: treating amorphous Atorvastatin hemi magnesium salt in water or a mixture of organic solvents and water; optionally heating the mixture with stirring followed by isolating the crystalline form of amorphous atorvastatin hemi magnesium salt by filtration; and drying to obtain crystalline atorvastatin hemi magnesium salt.
In another embodiment of the present invention wherein the solvent used is selected from a group comprising water or a mixture of organic solvents and water.
In yet another embodiment of the present invention wherein the organic solvent is selected from a group comprising hydroxylic, non-hydroxylic solvent or mixture thereof.
In still another embodiment of the present invention wherein the organic solvent is selected from a group comprising acetone, methanol, ethanol, ethyl acetate, isopropyl alcohol, THF, dichloromethane, t-butanol, iso-butanol, carbon tetrachloride, 1,4- dioxan, n-butanol, di-isopropyl ether, di-ethyl ether and mixtures thereof. In still another embodiment of the present invention wherein the mixture is heated to a temperature of about 400C.
In still another embodiment of the present invention wherein the crystalline form of atorvastatin magnesium is B4. In still another embodiment of the present invention wherein the crystalline form of atorvastatin magnesium is B5.
In still another embodiment of the present invention wherein the crystalline form of atorvastatin magnesium is B6.
The present invention is in relation to a crystalline atorvastatin hemi magnesium salt as claimed in any of the preceding claims having a particle size ranging from 1 micron to 150 microns.
The present invention is in relation to a pharmaceutical composition comprising crystalline atorvastatin magnesium form selected from a group comprising B4, B5, B6 and combinations thereof optionally along with pharmaceutically acceptable excipients. In another embodiment of the present invention wherein the excipients are selected from a group comprising granulating agents, binding agents, lubricating agents, disintegrating agents, sweetening agents, coloring agents, flavoring agents, coating agents, plasticizers, preservatives, suspending agents, emulsifying agents and spheronization agents. In still another embodiment of the present invention wherein said composition is formulated into dosage forms selected from a group comprising tablet, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsion in hard or soft gel capsules, syrups, elixirs, phyotceuticals, neutraceuticals and food stuffs. The present invention provides for new crystalline forms of atorvastatin hemi magnesium salt B4, B5 and B6 characterized by a X-ray powder diffraction pattern as depicted in Fig 1, 2 & 3 respectively.
In another aspect, the present invention provides new processes for the preparation of atorvastatin hemi magnesium salt forms B4, B5 and B6,
According to yet another aspect the invention provides pharmaceutical compositions and dosage forms comprising atorvastatin magnesium forms B4, B5 or B6.
A still further embodiment of the present invention relates to a method of treating hyperlipidemia or hypercholesteremia with a pharmaceutical composition containing a therapeutically effective amount of atorvastatin magnesium crystalline forms B4, B5 and B6.
Particle size of the crystalline forms atorvastatin hemi magnesium salt is about from 1 to 150 microns.
The present invention provides polymorphic forms of atorvastatin hemi magnesium salt, the present invention provides atorvastatin hemi magnesium salt in two new polymorphic forms denominated as crystalline forms" B4", " B5", and "B6".
The forms B4, B5 and B6 exhibit different physical characteristics which are evident from their X-ray powder diffraction patterns. While the invention will be described in conjunction with these specific embodiments, it will be understood that it is not intended to limit the invention to such specific embodiments. On the contrary, it is intended to cover alternatives, modifications, and equivalents as may be included within the spirit and scope of the invention as defined by the claims. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may be practiced without some or all of these specific details. In other instance a well known process operations have not been described in detail, in order not to obscure the present invention.
This invention is related to crystalline forms B4, B5 and B6 of atorvastatin hemi magnesium salt having the following chemical structure:
Formula I
The present invention is further directed to the processes for the production and isolation of forms of B4 , B5 or B6, to pharmaceutical compositions which include the crystalline forms B4 or B5, and a pharmaceutically acceptable carrier, and to a method of administering a therapeutic amount of the pharmaceutical composition for the treatment of hyperlipidemia and hypercholesterolemia. The B4, B5 and B6 forms of atorvastatin magnesium are useful as inhibitors of the enzyme HMG-CoA reductase, and therefore, are useful for treating hyperlipidemia and hypercholesterolemia. The B4, B5 and B6 forms are characterized by their distinctive X-ray powder diffractograms as depicted in Fig 1, 2 & 3 respectively. The present invention also provides for a method for the preparation of crystalline forms B4 and B5 of atorvastatin hemi magnesium salt. The method comprises exposing atorvastatin to different solvents and temperature conditions, which yield crystalline forms B4, B5 and B6.
Crystalline atorvastatin hemi magnesium salt form B4, B5 and B6 may be prepared under proper conditions. In particular, they can be prepared/ isolated by crystallization from aqueous, water-miscible, non- aqueous or non-polar solvents at a suitable temperature. Suitable solvents comprise water, acetone, methanol, ethanol, ethyl acetate, isopropyl alcohol, THF, dichloromethane, t-butanol, iso-butanol, carbon tetrachloride, 1,4-dioxan, n-butanol, di-isopropyl ether or di-ethyl ether. In one embodiment, amorphous form of atorvastatin hemi magnesium salt is treated with water or a mixture of two or more suitable solvents or mixture of a solvent and water under a suitable temperature range and the mixture can be then filtered and dried, preferably under vacuum, to obtain crystalline atorvastatin hemi magnesium salt.
As may be appreciated and cognized by a skilled artisan, the used solvents can be removed by various drying methods such as vacuum drying, rapid solvent evaporation , spray drying, freeze drying, solvent precipitation, roller drying and the like.
The present invention contemplates temperature variations during heating the mixture. Heating may be carried out at temperatures ranging from 4O0C to 1000C. According to a preferred embodiment, the heating temperature preferably ranges from 400C to 700C.
Amorphous form of atorvastatin hemi magnesium salt can be prepared from ATV-I boronate (formula II) by following the methods disclosed in WO 2007/063551.
The polymorph B4 may be obtained by stirring amorphous Atorvastatin hemi magnesium salt in a mixture of water and acetone followed by the separation of solids and drying. In the same manner stirring amorphous Atorvastatin hemi magnesium salt in water at reflection will produce crystalline form B5.
In accordance to the present invention, the method of making crystalline Atorvastatin hemi magnesium salt having at least one of the defining characteristics exemplified in the foregoing description include steps of: treating amorphous atorvastatin hemi magnesium salt in water or a mixture of organic solvents and water, optionally heating the mixture with stirring, isolating the crystalline form of amorphous atorvastatin hemi magnesium salt by filtration and subsequent drying.
According to yet another aspect, the polymorphs exemplified in invention may be formulated in the form of tablets, pills, powder mixtures, capsules, injectables, solutions, suspensions, suppositories, emulsions, dispersions, food premix, and in other suitable forms. It may also be manufactured in the form of sterile solid compositions, for example, freeze dried and, if desired, combined with other pharmaceutically acceptable excipients, carriers, diluents or adjuvants. According to a preferred aspect of the invention the particle size of crystalline atorvastatin hemi magnesium salt is about from 1 to 150 microns.
The technology of the instant Application is further elaborated with the help of following examples. However, the examples should not be construed to limit the scope of the invention.
Example-1: preparation of amorphous atorvastatin hemi magnesium salt (disclosed in WO 2007/063551)
Amorphous atorvastatin hemi magnesium salt was obtained by adding ATV-I boronate (formula II) to a mixture of water and methanol followed by the hydrolysis in the presence of NaOH solution at reflux temperature for about 4 h. The mass was cooled and washed with methyl-tert-butyl ether. The aqueous layer was subjected to vacuum at about 30 0C to obtain the solids and stirred at room temperature. The sodium salt obtained was filtered and dissolved in a mixture of ethyl acetate and water. The pH of the mass was adjusted to 8-8.5 using HCl solutions followed by the addition of magnesium acetate. The mass was heated to 40-45° C under stirring. The mass was cooled to room temperature and the organic layer was separated. The organic layer was washed with water and concentrated to syrup under vacuum followed by stripping off with methanol and concentrated to syrup. The syrup obtained was dried under vacuum at 40-50° C to obtain amorphous Atorvastatin hemi magnesium salt.
Example-2:
Amorphous atorvastatin hemi magnesium salt (2 g) was suspended in a mixture of acetone and water (1:1 mixture, 30 mL) and the resulting mass was stirred at room temperature for 65 h. The solids were filtered and dried under vacuum at 50-60° C for 12 h to obtained crystalline atorvastatin hemi magnesium salt (1.5 g). The crystals were characterized by a X-Ray diffraction pattern as depicted in Fig.l defining Form B4 having melting point of 155 °C.
Example-3:
Amorphous atorvastatin hemi magnesium salt (2 g) was suspended in water (30 mL) and resulting mass was stirred at reflux temperature for 48 h. The solids were filtered and dried under vacuum at 50-60° C for 12 h to obtain crystalline atorvastatin hemi magnesium salt (1.4 g). The crystals were characterized by a X-Ray diffraction pattern as depicted in Fig.2 defining Form B5 having melting point of 159 °C.
Example-4:
Atorvastatin magnesium amorphous (2 g, obtained above) was suspended in a mixture of ethanol and water (1 : 1 mixture, 30 mL) and resulting mass was stirred at 50-60° C
for about 72 h. The solids were filtered and dried under vacuum at 50-60° C for 12 h to obtain Atorvastatrn magnesium crystalline (1.2 g). The crystals were characterized by a X-Ray diffraction pattern as depicted in Fig.l defining Form B4 having melting point of 155 0C. Example-5:
Atorvastatin magnesium amorphous (2 g, obtained above) was suspended in a mixture of methanol and water (1 :1 mixture, 40 mL) and resulting mass was stirred at 50-60° C for about 72 h. The solids were filtered and dried under vacuum at 50-60° C for 12 h to obtain Atorvastatin magnesium crystalline (1.4 g). The crystals were characterized by a X-Ray diffraction pattern as depicted in Fig.3 defining Form B6 having melting point of 147 °C.
Claims
We Claim:
I . A crystalline form of atorvastatin hemi magnesium salt characterized by X-ray diffraction pattern comprising 2 theta values 3.49, 4.45, 5.32, 5.58, 6.99, 7.96, 8.30, 9.12, 11.96, 19.01 and 19.80 ± 0.2 2. A crystalline form of atorvastatin hemi magnesium salt characterized by X-ray diffraction pattern comprising 2 theta values 3.45, 5.19, 6.06, 6.87, 7.64, 8.38, 10.29, 11.22, 1 1.70, 12.11, 15.22, 18.1 1, 22.30, 22.84, 24.15and 27.73± 0.
2 ,
3. A crystalline form of atorvastatin hemi magnesium salt characterized by X-ray diffraction pattern comprising 2 theta values 3.59, 4.62, 5.34, 9.39 and 11.0 ± 0.2.
4. The crystalline form as claimed in claim 1, wherein the crystalline form is B4.
5. The crystalline form as claimed in claim 2, wherein the crystalline form is B5.
6. The crystalline form as claimed in claim 3, wherein the crystalline form is B6.
7. The crystalline form of atorvastatin magnesium as claimed in claims 1 to 6, wherein the melting point is in the range of 147°C to 168°C.
8. A process for preparation of crystalline atorvastatin hemi magnesium salt, wherein said process comprising steps of: a. treating amorphous Atorvastatin hemi magnesium salt in water or a mixture of organic solvents and water, b. optionally heating the mixture with stirring followed by isolating the crystalline form of amorphous atorvastatin hemi magnesium salt by filtration; and c. drying to obtain crystalline atorvastatin hemi magnesium salt.
9. The process as claimed in claim 8, wherein the solvent used is selected from a group comprising water or a mixture of organic solvents and water.
10. The process as claimed in claim 8, wherein the organic solvent is selected from a group comprising hydroxylic, non-hydroxylic solvent or mixture thereof.
I I. The process as claimed in claim 8, wherein the organic solvent is selected from a group comprising acetone, methanol, ethanol, ethyl acetate, isopropyl alcohol, THF, dichloromethane, t-butanol, iso-butanol, carbon tetrachloride, 1,4-dioxan, n-butanol, di-isopropyl ether, di-ethyl ether and mixtures thereof.
12. The process as claimed in claim 8, wherein the mixture is heated to a temperature of about 400C.
13. The process as claimed in claim 8, wherein the crystalline form of atorvastatin magnesium is B4.
14. The process as claimed in claim 8, wherein the crystalline form of atorvastatin magnesium is B5.
15. The process as claimed in claim 8, wherein the crystalline form of atorvastatin magnesium is B6.
16. A crystalline atorvastatin hemi magnesium salt as claimed in any of the preceding claims having a particle size ranging from 1 micron to 150 microns.
17. A pharmaceutical composition comprising crystalline atorvastatin magnesium form selected from a group comprising B4, B5, B6 and combinations thereof optionally along with pharmaceutically acceptable excipients.
18. The pharmaceutical composition as claimed in claim 17, wherein the excipients are selected from a group comprising granulating agents, binding agents, lubricating agents, disintegrating agents, sweetening agents, coloring agents, flavoring agents, coating agents, plasticizers, preservatives, suspending agents, emulsifying agents and spheronization agents.
19. The pharmaceutical composition as claimed in claim 17, wherein said composition is formulated into dosage forms selected from a group comprising tablet, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsion in hard or soft gel capsules, syrups, elixirs, phyotceuticals, neutraceuticals and food stuffs.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN2668CH2007 | 2007-11-16 | ||
| IN2668/CHE/2007 | 2007-11-16 |
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| WO2009063476A1 true WO2009063476A1 (en) | 2009-05-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/IN2007/000623 WO2009063476A1 (en) | 2007-11-16 | 2007-12-31 | A crystalline form of atorvastatin hemi magnesium salt and a process thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2130819A3 (en) * | 2008-04-10 | 2009-12-23 | Ranbaxy Laboratories Limited | Crystalline forms of atorvastatin magnesium |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5273995A (en) * | 1989-07-21 | 1993-12-28 | Warner-Lambert Company | [R-(R*R*)]-2-(4-fluorophenyl)-β,δ-dihydroxy-5-(1-methylethyl-3-phenyl-4-[(phenylamino) carbonyl]- 1H-pyrrole-1-heptanoic acid, its lactone form and salts thereof |
| WO2006021969A1 (en) * | 2004-08-27 | 2006-03-02 | Biocon Limited | Process for atorvastatin calcium amorphous |
| WO2006117761A2 (en) * | 2005-05-03 | 2006-11-09 | Ranbaxy Laboratories Limited | Magnesium salts of hmg-coa reductase inhibitors |
| WO2007057755A1 (en) * | 2005-11-21 | 2007-05-24 | Warner-Lambert Company Llc | Novel forms of [r-(r*,r*)]-2-(4-fluorophenyl)-b,b-dihydroxy-5-(1-methylethyl)-3-phenyl-4-[(phenylamino)carbonyl]-1h-pyrrole-1-hept anoic acid magnesium |
| WO2007063551A1 (en) * | 2005-11-29 | 2007-06-07 | Biocon Limited | POLYMORPHS OF [R-(R*, R*)]-2-(4-FLUOROPHENYL)-β,δ-DIHYDROXY-5-(l-METHYLETHYL)-3-PHENYL-4-[(PHENYLAMINO)CARBONYL]-lH-PYRROLE-l-HEPTANOIC ACID MAGNESIUM SALT (2: 1) |
| WO2007099552A2 (en) * | 2006-03-02 | 2007-09-07 | Matrix Labaratories Ltd | Novel crystalline form of atovastatin hemi-magnesium |
| WO2007118873A2 (en) * | 2006-04-14 | 2007-10-25 | Krka, Tovarna Zdravil, D.D., Novo Mesto | Polymorphs of atorvastatin sodium and magnesium salts |
| WO2007132472A1 (en) * | 2006-05-11 | 2007-11-22 | Biocon Limited | A crystalline form b4 of atorvastatin magnesium and a process thereof |
-
2007
- 2007-12-31 WO PCT/IN2007/000623 patent/WO2009063476A1/en active Application Filing
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5273995A (en) * | 1989-07-21 | 1993-12-28 | Warner-Lambert Company | [R-(R*R*)]-2-(4-fluorophenyl)-β,δ-dihydroxy-5-(1-methylethyl-3-phenyl-4-[(phenylamino) carbonyl]- 1H-pyrrole-1-heptanoic acid, its lactone form and salts thereof |
| WO2006021969A1 (en) * | 2004-08-27 | 2006-03-02 | Biocon Limited | Process for atorvastatin calcium amorphous |
| WO2006117761A2 (en) * | 2005-05-03 | 2006-11-09 | Ranbaxy Laboratories Limited | Magnesium salts of hmg-coa reductase inhibitors |
| WO2007057755A1 (en) * | 2005-11-21 | 2007-05-24 | Warner-Lambert Company Llc | Novel forms of [r-(r*,r*)]-2-(4-fluorophenyl)-b,b-dihydroxy-5-(1-methylethyl)-3-phenyl-4-[(phenylamino)carbonyl]-1h-pyrrole-1-hept anoic acid magnesium |
| WO2007063551A1 (en) * | 2005-11-29 | 2007-06-07 | Biocon Limited | POLYMORPHS OF [R-(R*, R*)]-2-(4-FLUOROPHENYL)-β,δ-DIHYDROXY-5-(l-METHYLETHYL)-3-PHENYL-4-[(PHENYLAMINO)CARBONYL]-lH-PYRROLE-l-HEPTANOIC ACID MAGNESIUM SALT (2: 1) |
| WO2007099552A2 (en) * | 2006-03-02 | 2007-09-07 | Matrix Labaratories Ltd | Novel crystalline form of atovastatin hemi-magnesium |
| WO2007118873A2 (en) * | 2006-04-14 | 2007-10-25 | Krka, Tovarna Zdravil, D.D., Novo Mesto | Polymorphs of atorvastatin sodium and magnesium salts |
| WO2007132472A1 (en) * | 2006-05-11 | 2007-11-22 | Biocon Limited | A crystalline form b4 of atorvastatin magnesium and a process thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2130819A3 (en) * | 2008-04-10 | 2009-12-23 | Ranbaxy Laboratories Limited | Crystalline forms of atorvastatin magnesium |
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