WO2009053870A3 - Device and method for the monitoring of the movement of cells - Google Patents

Device and method for the monitoring of the movement of cells Download PDF

Info

Publication number
WO2009053870A3
WO2009053870A3 PCT/IB2008/054195 IB2008054195W WO2009053870A3 WO 2009053870 A3 WO2009053870 A3 WO 2009053870A3 IB 2008054195 W IB2008054195 W IB 2008054195W WO 2009053870 A3 WO2009053870 A3 WO 2009053870A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
stimulant
movement
detection
electrical
Prior art date
Application number
PCT/IB2008/054195
Other languages
French (fr)
Other versions
WO2009053870A2 (en
Inventor
Stefano Cattaneo
Murray F Gillies
Simone I E Vulto
Hoog Thomas J De
Dennis Merkle
Mark T Johnson
Original Assignee
Koninkl Philips Electronics Nv
Stefano Cattaneo
Murray F Gillies
Simone I E Vulto
Hoog Thomas J De
Dennis Merkle
Mark T Johnson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninkl Philips Electronics Nv, Stefano Cattaneo, Murray F Gillies, Simone I E Vulto, Hoog Thomas J De, Dennis Merkle, Mark T Johnson filed Critical Koninkl Philips Electronics Nv
Publication of WO2009053870A2 publication Critical patent/WO2009053870A2/en
Publication of WO2009053870A3 publication Critical patent/WO2009053870A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/028—Non-uniform field separators using travelling electric fields, i.e. travelling wave dielectrophoresis [TWD]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Genetics & Genomics (AREA)
  • Electrochemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention relates to a device for the monitoring of very small movements of cells, in particular when undergoing chemotaxis. The invention makes use of various ways of electrical detection, like capacitance or impedance measurements. In this way the detection of the movement of cells can be done in situ, which accelerates the chemotaxis assay and increases the accuracy of the measurements. The device according to the present invention particularly comprises a stimulant gradient chamber (1) for providing a concentration gradient of a stimulant, said stimulant gradient chamber (1) having a cell inlet (12) for the inlet of cells (C), - an electrical sensor (2) placed in a measurement area for measuring an electrical parameter which changes in response to a movement of cells (C) in the surrounding of said sensor (2) and for generating a detection signal, and a signal evaluation unit (3) for evaluating said detection signal.
PCT/IB2008/054195 2007-10-22 2008-10-13 Device and method for the monitoring of the movement of cells WO2009053870A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07119012 2007-10-22
EP07119012.8 2007-10-22

Publications (2)

Publication Number Publication Date
WO2009053870A2 WO2009053870A2 (en) 2009-04-30
WO2009053870A3 true WO2009053870A3 (en) 2009-06-11

Family

ID=40418912

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2008/054195 WO2009053870A2 (en) 2007-10-22 2008-10-13 Device and method for the monitoring of the movement of cells

Country Status (1)

Country Link
WO (1) WO2009053870A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201312035D0 (en) * 2013-07-04 2013-08-21 Cytomos Ltd Biological sensing apparatus
US10444045B2 (en) 2018-03-08 2019-10-15 International Business Machines Corporation 2D nanoparticle motion sensing methods and structures
US10422672B1 (en) 2018-03-08 2019-09-24 International Business Machines Corporation 2D nanoparticle motion sensing methods and structures
US10900884B2 (en) 2018-03-08 2021-01-26 International Business Machines Corporation 2D nanoparticle motion sensing methods and structures

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000073799A1 (en) * 1999-06-01 2000-12-07 Caliper Technologies Corp. Microscale assays and microfluidic devices for transporter, gradient induced, and binding activities
WO2002042766A2 (en) * 2000-10-26 2002-05-30 University Of Connecticut A system and method for investigating the effect of chemical and other factors on cell movement
US20040142411A1 (en) * 2000-11-08 2004-07-22 Kirk Gregory L. Biological assays using gradients formed in microfluidic systems
WO2005085852A1 (en) * 2004-03-03 2005-09-15 Humboldt-Universität Zu Berlin Method and device for measuring a physical and/or a chemical property of a biological particle in a microsystem
WO2006025858A2 (en) * 2004-02-17 2006-03-09 Yale University Microfabricated cellular traps based on three-dimensional micro-scale flow geometries

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000073799A1 (en) * 1999-06-01 2000-12-07 Caliper Technologies Corp. Microscale assays and microfluidic devices for transporter, gradient induced, and binding activities
WO2002042766A2 (en) * 2000-10-26 2002-05-30 University Of Connecticut A system and method for investigating the effect of chemical and other factors on cell movement
US20040142411A1 (en) * 2000-11-08 2004-07-22 Kirk Gregory L. Biological assays using gradients formed in microfluidic systems
WO2006025858A2 (en) * 2004-02-17 2006-03-09 Yale University Microfabricated cellular traps based on three-dimensional micro-scale flow geometries
WO2005085852A1 (en) * 2004-03-03 2005-09-15 Humboldt-Universität Zu Berlin Method and device for measuring a physical and/or a chemical property of a biological particle in a microsystem

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
EMERSON D ET AL: "A diffusion gradient chamber for studying microbial behavior and separating microorganisms", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 60, no. 4, 1 April 1994 (1994-04-01), pages 1269 - 1278, XP002361173, ISSN: 0099-2240 *
ESTRELA P ET AL: "Electrical detection of biomolecular interactions with metal-insulator-semiconductor diodes", BIOSENSORS & BIOELECTRONICS, vol. 20, no. 8, 15 February 2005 (2005-02-15), pages 1580 - 1586, XP004697377, ISSN: 0956-5663 *
PETEU SERBAN F ET AL: "In situ mapping of community-level cellular response with catalytic microbiosensors", BIOSENSORS AND BIOELECTRONICS, vol. 13, no. 11, 15 November 1998 (1998-11-15), pages 1197 - 1203, XP002520484, ISSN: 0956-5663 *

Also Published As

Publication number Publication date
WO2009053870A2 (en) 2009-04-30

Similar Documents

Publication Publication Date Title
DE60335950D1 (en) HYBRID MICROOF SENSORS
DE602006009637D1 (en) INSPECTION SYSTEM AND DEVICE
DE10253154A8 (en) Measuring device for determining an analyte in a liquid sample
ATE462350T1 (en) METHOD AND DEVICE FOR CONTINUOUS MONITORING THE CONCENTRATION OF AN ANALYTE
WO2009031761A4 (en) Biosensor and readout meter
WO2012054758A3 (en) Apparatus and method for measuring binding kinetics with a resonating sensor
EP1742045A4 (en) Method for measuring blood components and biosensor and measuring instrument for use therein
ATE532063T1 (en) ELECTROCHEMICAL BIOSENSOR
EP2382556A4 (en) Method and device for early signal attenuation detection using blood glucose measurements
ATE472110T1 (en) SENSOR DEVICE AND METHOD FOR DETECTING MAGNETIC PARTICLES
ATE514955T1 (en) METHOD AND APPARATUS FOR EVALUATION OF THE DEGREE OF SURFACE CONTAMINATION OF A MEDIUM/HIGH VOLTAGE OUTDOOR INSULATOR
ATE551947T1 (en) BLOOD SUGAR SENSOR
ATE383567T1 (en) DEVICE, SENSOR ARRANGEMENT AND METHOD FOR CAPACITIVE POSITION DETECTION OF A TARGET OBJECT
WO2007094805A3 (en) Method for electrocatalytic protein detection
CN205607982U (en) Meat quality detection device based on smell and impedance
MY148063A (en) A method of determining the effect of a spill on a marine environment
WO2009053870A3 (en) Device and method for the monitoring of the movement of cells
WO2007059433A3 (en) Sheet conductance/resistance measurement system
TW200700750A (en) Methods and apparatus for non-contact testing and diagnosing of open connections on non-probed nodes
DE50205440D1 (en) METHOD AND DEVICE FOR DETECTING METHYL ISOTHYOCYANATE IN AIR TESTS
MX2021015167A (en) Interrogation of capillary-limited sensors.
CN102520134B (en) Environmental probe capable of in-situ testing pH value of soil body
WO2010076556A3 (en) A conductivity measurement cell
WO2006087697A3 (en) Testing device for examining fluid specimens particularly urine
WO2009111163A3 (en) System and method for detecting local mechanical stress in integrated devices

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08807969

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 08807969

Country of ref document: EP

Kind code of ref document: A2