WO2009051226A1 - Method for production of stable mutant protein - Google Patents

Method for production of stable mutant protein Download PDF

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Publication number
WO2009051226A1
WO2009051226A1 PCT/JP2008/068861 JP2008068861W WO2009051226A1 WO 2009051226 A1 WO2009051226 A1 WO 2009051226A1 JP 2008068861 W JP2008068861 W JP 2008068861W WO 2009051226 A1 WO2009051226 A1 WO 2009051226A1
Authority
WO
WIPO (PCT)
Prior art keywords
amino acid
protein
acid residue
mutation
target site
Prior art date
Application number
PCT/JP2008/068861
Other languages
French (fr)
Japanese (ja)
Inventor
Shinya Honda
Yoshito Sawada
Hiroyuki Matsumaru
Original Assignee
National Institute Of Advanced Industrial Science And Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Institute Of Advanced Industrial Science And Technology filed Critical National Institute Of Advanced Industrial Science And Technology
Publication of WO2009051226A1 publication Critical patent/WO2009051226A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Disclosed is a generally applicable method for improving the stability of a protein molecule, including thermal stability, chemical stability against a modifying agent and resistance against a protease, compared with the intrinsic stability of the protein molecule, without deteriorating the original functions of the protein molecule. The method is for producing a useful mutant form of a protein, and comprises the steps of: selecting, as a target site for mutation, an amino acid residue located on a partial segment whose three-dimensional structure is stabilized by the interaction among adjacent amino acid residues located on the amino acid sequence for the protein, by using a three-dimensional structure coordinate data from the protein; selecting, as other amino acid residue by which the target site for mutation is to be substituted, an amino acid residue having high frequency of occurrence in a conformation that is the same as or similar to the conformation of the partial segment; and substituting the target site for mutation by the selected amino acid residue.
PCT/JP2008/068861 2007-10-19 2008-10-17 Method for production of stable mutant protein WO2009051226A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007272442A JP5382675B2 (en) 2007-10-19 2007-10-19 Method for producing stable mutant protein
JP2007-272442 2007-10-19

Publications (1)

Publication Number Publication Date
WO2009051226A1 true WO2009051226A1 (en) 2009-04-23

Family

ID=40567487

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/068861 WO2009051226A1 (en) 2007-10-19 2008-10-17 Method for production of stable mutant protein

Country Status (2)

Country Link
JP (1) JP5382675B2 (en)
WO (1) WO2009051226A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140221613A1 (en) 2011-08-04 2014-08-07 Daicel Corporation Novel modified protein comprising tandem-type multimer of mutant extracellular domain of protein g
WO2014021240A1 (en) 2012-08-03 2014-02-06 独立行政法人産業技術総合研究所 Scavenger containing protein formed from tandem-type multimer of mutant extracellular domain of protein g
JPWO2015050153A1 (en) 2013-10-04 2017-03-09 国立研究開発法人産業技術総合研究所 A protein comprising a plurality of domains having an affinity for a protein having an Fc portion of immunoglobulin G (IgG) and linked by a linker.

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004272867A (en) * 2003-03-10 2004-09-30 Pro Device:Kk Software for secondary-structure-stabilized design of protein

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5312901A (en) * 1986-02-14 1994-05-17 Pharmacia Lkb Biotechnology Ab Cloned streptococcal genes encoding protein G and their use to construct recombinant microorganisms to produce protein G
DE69733467T2 (en) * 1996-01-25 2006-03-23 Kaneka Corp. ADSORBTIONSVORRICHTUNG AND ABSORBENS FOR IMMUNOBLOBINE AND THEIR COMPLEXES
JP3964087B2 (en) * 1999-12-24 2007-08-22 株式会社カネカ Method and apparatus for calculating optimized solution of multiple mutant protein amino acid sequence, and storage medium storing program for executing processing of this method
JP2005293296A (en) * 2004-03-31 2005-10-20 Shionogi & Co Ltd Method for evaluating interaction of sugar with protein

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004272867A (en) * 2003-03-10 2004-09-30 Pro Device:Kk Software for secondary-structure-stabilized design of protein

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FAHNESTOCK S.R. ET AL.: "Gene for an immunoglobulin-binding protein from a group G streptococcus.", J. BACTERIOL., vol. 167, no. 3, 1986, pages 870 - 880 *
HONDA S. ET AL.: "10 residue folded peptide designed by segment statistics.", STRUCTURE, vol. 12, no. 8, 2004, pages 1507 - 1518 *
HONDA S. ET AL.: "Structural diversity of protein segments follows a power-law distribution.", BIOPHYS. J., vol. 91, 2006, pages 1213 - 1223 *

Also Published As

Publication number Publication date
JP2009095322A (en) 2009-05-07
JP5382675B2 (en) 2014-01-08

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