WO2009044336A3 - Method for producing diterpenes - Google Patents

Method for producing diterpenes Download PDF

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Publication number
WO2009044336A3
WO2009044336A3 PCT/IB2008/053973 IB2008053973W WO2009044336A3 WO 2009044336 A3 WO2009044336 A3 WO 2009044336A3 IB 2008053973 W IB2008053973 W IB 2008053973W WO 2009044336 A3 WO2009044336 A3 WO 2009044336A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
present
polypeptide
diterpene
provides
Prior art date
Application number
PCT/IB2008/053973
Other languages
French (fr)
Other versions
WO2009044336A2 (en
WO2009044336A9 (en
Inventor
Michel Schalk
Original Assignee
Firmenich & Cie
Michel Schalk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Firmenich & Cie, Michel Schalk filed Critical Firmenich & Cie
Publication of WO2009044336A2 publication Critical patent/WO2009044336A2/en
Publication of WO2009044336A3 publication Critical patent/WO2009044336A3/en
Publication of WO2009044336A9 publication Critical patent/WO2009044336A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/07—Diphosphoric monoester hydrolases (3.1.7)
    • C12Y301/07004—Sclareol cyclase (3.1.7.4)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides a method of producing diterpene compounds, said method comprising contacting a particular polypeptide having a diterpene synthase activity with the diterpene precursor geranylgeranyl pyrophosphate (GGPP). In particular, said method may be carried out in vitro or in vivo to produce labdenediol diphosphate, labdenediol and/or sclareol, which are very useful compounds in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of the polypeptide used in the method. A nucleic acid derived from Salvia sclarea and encoding the polypeptide of the invention, an expression vector containing said nucleic acid, as well as a non-human organism or a cell transformed to harbor the same nucleic acid, are also part of the present invention.
PCT/IB2008/053973 2007-10-05 2008-09-30 Method for producing diterpenes WO2009044336A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07117986 2007-10-05
EP07117986.5 2007-10-05

Publications (3)

Publication Number Publication Date
WO2009044336A2 WO2009044336A2 (en) 2009-04-09
WO2009044336A3 true WO2009044336A3 (en) 2009-05-28
WO2009044336A9 WO2009044336A9 (en) 2009-07-09

Family

ID=39166977

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2008/053973 WO2009044336A2 (en) 2007-10-05 2008-09-30 Method for producing diterpenes

Country Status (1)

Country Link
WO (1) WO2009044336A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN2012DN00403A (en) 2009-06-29 2015-08-21 Univ Western Australia
CN104053770A (en) 2011-10-19 2014-09-17 凯金公司 Methods for producing cinnamolide and/or drimendiol
US8889381B2 (en) 2011-11-21 2014-11-18 University Of British Columbia Diterpene synthases and method for producing diterpenoids
WO2013110191A1 (en) 2012-01-23 2013-08-01 The University Of British Columbia Abc trepenoid transporters and methods of using the same
WO2014022434A1 (en) 2012-07-30 2014-02-06 Allylix, Inc. Sclareol and labdenediol diphosphate synthase polypeptides encoding nucleic acid molecules and uses thereof
EP2886647A1 (en) * 2013-12-20 2015-06-24 Technical University of Denmark Polypeptides with diterpene synthase activity
WO2015091943A1 (en) * 2013-12-20 2015-06-25 Technical Unversity Of Denmark Stereo-specific synthesis of (13r)-manoyl oxide
EP3099802A1 (en) 2014-01-31 2016-12-07 The University of Copenhagen Biosynthesis of forskolin and related compounds
US10208326B2 (en) 2014-11-13 2019-02-19 Evolva Sa Methods and materials for biosynthesis of manoyl oxide
WO2016166243A1 (en) * 2015-04-15 2016-10-20 Evolva Sa Biosynthesis of acetylated 13r-mo and related compounds
US10982232B2 (en) 2016-07-27 2021-04-20 The Regents Of The University Of California Diterpene synthases and their use for production of diterpenes
ES2899121T3 (en) * 2016-12-22 2022-03-10 Firmenich & Cie Hand oil production
CN115074375B (en) * 2022-03-09 2024-04-05 中国医学科学院药用植物研究所 Salvia 2-ketoglutarate-dependent dioxygenase gene and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040072323A1 (en) * 2001-01-05 2004-04-15 Matsuda Seiichi P.T. Diterpene-producing unicellular organism

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040072323A1 (en) * 2001-01-05 2004-04-15 Matsuda Seiichi P.T. Diterpene-producing unicellular organism

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BANTHORPE D V ET AL: "ACCUMULATION OF THE ANTI-FUNGAL DITERPENE SCLAREOL BY CELL CULTURES OF SALVIA SCLAREA AND NICOTIANA GLUTINOSA", PHYTOCHEMISTRY, PERGAMON PRESS, GB, vol. 29, no. 7, January 1990 (1990-01-01), pages 2145 - 2148, XP000671796, ISSN: 0031-9422 *
CYR ANTHONY ET AL: "A modular approach for facile biosynthesis of labdane-related diterpenes.", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 129, no. 21, 30 May 2007 (2007-05-30), pages 6684 - 6685, XP002473660, ISSN: 0002-7863 *
DATABASE EBI [online] 1 May 2000 (2000-05-01), IMAI R: "Tomato cDNA for copalyl diphosphate synthase", XP002473661, Database accession no. Q9ST35 *
DATABASE EBI [online] 5 October 1999 (1999-10-05), IMAI R: "Tomato cDNA for copalyl diphosphate synthase", XP002473664, Database accession no. AB015675 *

Also Published As

Publication number Publication date
WO2009044336A2 (en) 2009-04-09
WO2009044336A9 (en) 2009-07-09

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