WO2009044156A3 - Methods of identifying modulators of an ehd polypeptide - Google Patents

Methods of identifying modulators of an ehd polypeptide Download PDF

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Publication number
WO2009044156A3
WO2009044156A3 PCT/GB2008/003355 GB2008003355W WO2009044156A3 WO 2009044156 A3 WO2009044156 A3 WO 2009044156A3 GB 2008003355 W GB2008003355 W GB 2008003355W WO 2009044156 A3 WO2009044156 A3 WO 2009044156A3
Authority
WO
WIPO (PCT)
Prior art keywords
ehd
polypeptide
methods
modulator
samples
Prior art date
Application number
PCT/GB2008/003355
Other languages
French (fr)
Other versions
WO2009044156A2 (en
Inventor
Harvey Mcmahon
Gary Doherty
Richard Lundmark
Oli Daumke
Original Assignee
Medical Res Council
Harvey Mcmahon
Gary Doherty
Richard Lundmark
Oli Daumke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Res Council, Harvey Mcmahon, Gary Doherty, Richard Lundmark, Oli Daumke filed Critical Medical Res Council
Priority to EP08806500A priority Critical patent/EP2205756A2/en
Priority to US12/680,347 priority patent/US20120214173A1/en
Publication of WO2009044156A2 publication Critical patent/WO2009044156A2/en
Publication of WO2009044156A3 publication Critical patent/WO2009044156A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a method of identifying a modulator of an EHD family polypeptide, said method comprising (i) providing a first and second sample of an EHD polypeptide; (ii) contacting said first sample with a candidate modulator; (iii) contacting said first and second samples with ATP; and (iv) monitoring ATP hydrolysis in said first and second samples, wherein a difference between the ATP hydrolysis in said first and second samples identifies said candidate modulator as a modulator of an EHD family polypeptide. The invention also relates to methods of modelling molecules and to various EHD mutant polypeptides.
PCT/GB2008/003355 2007-10-02 2008-10-01 Methods of identifying modulators of an ehd polypeptide WO2009044156A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP08806500A EP2205756A2 (en) 2007-10-03 2008-10-01 Methods of identifying modulators of an ehd polypeptide
US12/680,347 US20120214173A1 (en) 2007-10-02 2008-10-01 Methods of Identifying Modulators of an Ehd Polypeptide

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0719252A GB2453341B (en) 2007-10-03 2007-10-03 Methods of identifying modulators of an EHD family polypeptide
GB0719252.9 2007-10-03

Publications (2)

Publication Number Publication Date
WO2009044156A2 WO2009044156A2 (en) 2009-04-09
WO2009044156A3 true WO2009044156A3 (en) 2009-07-23

Family

ID=38739010

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2008/003355 WO2009044156A2 (en) 2007-10-02 2008-10-01 Methods of identifying modulators of an ehd polypeptide

Country Status (3)

Country Link
EP (1) EP2205756A2 (en)
GB (1) GB2453341B (en)
WO (1) WO2009044156A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102787122B (en) * 2012-06-21 2013-06-19 南京农业大学 Gene OsEHD4 for controlling paddy rice heading stage and mutant and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020115069A1 (en) * 1997-02-21 2002-08-22 Mia Horowitz Eh domain containing genes and proteins
WO2003091390A2 (en) * 2001-11-14 2003-11-06 Digital Gene Technologies, Inc. Regulation of gene expression by neuromodulators and discovery of a novel ngf-induced protein termed pincher
WO2007079581A1 (en) * 2006-01-09 2007-07-19 University Of Manitoba Prohibitin attenuates insulin-stimulated glucose and fatty acid oxidation in adipose tissue by inhibition of pyruvate carobxylase

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020115069A1 (en) * 1997-02-21 2002-08-22 Mia Horowitz Eh domain containing genes and proteins
WO2003091390A2 (en) * 2001-11-14 2003-11-06 Digital Gene Technologies, Inc. Regulation of gene expression by neuromodulators and discovery of a novel ngf-induced protein termed pincher
WO2007079581A1 (en) * 2006-01-09 2007-07-19 University Of Manitoba Prohibitin attenuates insulin-stimulated glucose and fatty acid oxidation in adipose tissue by inhibition of pyruvate carobxylase

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
DAUMKE OLIVER ET AL: "Architectural and mechanistic insights into an EHD ATPase involved in membrane remodelling", NATURE (LONDON), vol. 449, no. 7164, 3 October 2007 (2007-10-03), pages 923, XP002529189, ISSN: 0028-0836 *
GEORGE M ET AL: "Shared as well as distinct roles of EHD proteins revealed by biochemical and functional comparisons in mammalian cells and C. elegans", BMC CELL BIOLOGY 2007 GB, vol. 8, 18 January 2007 (2007-01-18), XP002513318, ISSN: 1471-2121 *
GUILHERME ADILSON ET AL: "Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated GLUT4 recycling in 3T3-L1 adipocytes.", THE JOURNAL OF BIOLOGICAL CHEMISTRY 17 SEP 2004, vol. 279, no. 38, 17 September 2004 (2004-09-17), pages 40062 - 40075, XP002513317, ISSN: 0021-9258 *
KIEKEN FABIEN ET AL: "EH domain of EHD1", JOURNAL OF BIOMOLECULAR NMR, vol. 39, no. 4, 26 September 2007 (2007-09-26), pages 323 - 329, XP019553160, ISSN: 0925-2738 *
LEACH A: "Molecular Modelling, Principles and Applications; The use of molecular modelling and chemoinformatics to discover and design new molecules", 1 January 2001, MOLECULAR MODELLING: PRINCIPLES AND APPLICATIONS, XX, XX, PAGE(S) 640 - 726, XP002444241 *
LEE D -W ET AL: "ATP binding regulates oligomerization and endosome association of RME-1 family proteins", JOURNAL OF BIOLOGICAL CHEMISTRY 20050429 US, vol. 280, no. 17, 29 April 2005 (2005-04-29), pages 17213 - 17220, XP002513315, ISSN: 0021-9258 *
MONTESINOS ET AL: "Recycling and EH domain proteins at the synapse", BRAIN RESEARCH REVIEWS, ELSEVIER, vol. 49, no. 2, 1 September 2005 (2005-09-01), pages 416 - 428, XP005029083, ISSN: 0165-0173 *
NASLAVSKY N ET AL: "C-terminal EH-domain-containing proteins: Consensus for a role in endocytic trafficking, EH?", JOURNAL OF CELL SCIENCE 20050915 GB, vol. 118, no. 18, 15 September 2005 (2005-09-15), pages 4093 - 4101, XP002513319, ISSN: 0021-9533 *
PARK S Y ET AL: "EHD2 interacts with the insulin-responsive glucose transporter (GLUT4) in rat adipocytes and may participate in insulin-induced GLUT4 recruitment", BIOCHEMISTRY 20040615 US, vol. 43, no. 23, 15 June 2004 (2004-06-15), pages 7552 - 7562, XP002513316, ISSN: 0006-2960 *

Also Published As

Publication number Publication date
GB2453341B (en) 2010-08-25
GB2453341A (en) 2009-04-08
EP2205756A2 (en) 2010-07-14
GB0719252D0 (en) 2007-11-14
WO2009044156A2 (en) 2009-04-09

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