WO2009041409A1 - クレアチニンアミドヒドロラーゼ改変体 - Google Patents

クレアチニンアミドヒドロラーゼ改変体 Download PDF

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Publication number
WO2009041409A1
WO2009041409A1 PCT/JP2008/067145 JP2008067145W WO2009041409A1 WO 2009041409 A1 WO2009041409 A1 WO 2009041409A1 JP 2008067145 W JP2008067145 W JP 2008067145W WO 2009041409 A1 WO2009041409 A1 WO 2009041409A1
Authority
WO
WIPO (PCT)
Prior art keywords
amino acid
acid residue
creatinine amidohydrolase
residue located
amidohydrolase
Prior art date
Application number
PCT/JP2008/067145
Other languages
English (en)
French (fr)
Inventor
Rie Hirao
Original Assignee
Toyo Boseki Kabushiki Kaisha
Kitabayashi, Masao
Nishiya, Yoshiaki
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2007250878A external-priority patent/JP2009077684A/ja
Priority claimed from JP2007250876A external-priority patent/JP2009077682A/ja
Priority claimed from JP2007250877A external-priority patent/JP2009077683A/ja
Priority claimed from JP2007250874A external-priority patent/JP5130479B2/ja
Priority claimed from JP2007250875A external-priority patent/JP5130480B2/ja
Application filed by Toyo Boseki Kabushiki Kaisha, Kitabayashi, Masao, Nishiya, Yoshiaki filed Critical Toyo Boseki Kabushiki Kaisha
Priority to EP08833630.0A priority Critical patent/EP2202304B1/en
Publication of WO2009041409A1 publication Critical patent/WO2009041409A1/ja

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

【課題】公知のクレアチニンアミドヒドロラーゼの欠点を克服し、よりキレート剤に対する耐性が向上した、あるいは、より比活性が向上したクレアチニンアミドヒドロラーゼを提供すること。 【解決手段】シュードモナス・プチダ由来クレアチニンアミドヒドロラーゼのアミノ酸配列の１７５位、１２４位、７８位、１２２位のうちのいずれか１つ以上、あるいはそれと同等の位置のアミノ酸を他のアミノ酸に置換することを特徴とするクレアチニンアミドヒドロラーゼのキレート剤耐性を向上させる方法、もしくは、４３位、あるいはそれと同等の位置のアミノ酸を他のアミノ酸に置換することを特徴とするクレアチニンアミドヒドロラーゼの比活性を向上させる方法。
PCT/JP2008/067145 2007-09-27 2008-09-24 クレアチニンアミドヒドロラーゼ改変体 WO2009041409A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP08833630.0A EP2202304B1 (en) 2007-09-27 2008-09-24 Modified creatinine amidohydrolase

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
JP2007-250875 2007-09-27
JP2007250878A JP2009077684A (ja) 2007-09-27 2007-09-27 キレート剤耐性が向上したクレアチニンアミドヒドロラーゼ改変体
JP2007-250878 2007-09-27
JP2007250876A JP2009077682A (ja) 2007-09-27 2007-09-27 キレート剤耐性が向上したクレアチニンアミドヒドロラーゼ改変体
JP2007250877A JP2009077683A (ja) 2007-09-27 2007-09-27 キレート剤耐性が向上したクレアチニンアミドヒドロラーゼ改変体
JP2007250874A JP5130479B2 (ja) 2007-09-27 2007-09-27 クレアチニンアミドヒドロラーゼの比活性を向上させる方法
JP2007-250877 2007-09-27
JP2007250875A JP5130480B2 (ja) 2007-09-27 2007-09-27 キレート剤耐性が向上したクレアチニンアミドヒドロラーゼ改変体
JP2007-250876 2007-09-27
JP2007-250874 2007-09-27

Publications (1)

Publication Number Publication Date
WO2009041409A1 true WO2009041409A1 (ja) 2009-04-02

Family

ID=40511294

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/067145 WO2009041409A1 (ja) 2007-09-27 2008-09-24 クレアチニンアミドヒドロラーゼ改変体

Country Status (2)

Country Link
EP (1) EP2202304B1 (ja)
WO (1) WO2009041409A1 (ja)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102253041B (zh) * 2011-06-20 2013-02-20 董理 一种检测肌酐的试剂盒

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS51115989A (en) 1975-04-05 1976-10-13 Noda Sangyo Kagaku Kenkyusho Process for preparing creatinine amide hydrolase
JP2527035B2 (ja) 1989-06-16 1996-08-21 東洋紡績株式会社 クレアチニン・アミドヒドロラ―ゼをコ―ドする遺伝子を含有するｄｎａ断片、該ｄｎａ断片を有する組換えベクタ―及び該組換えベクタ―を有する形質転換体、並びにクレアチニン・アミドヒドロラ―ゼの製造方法
JPH09154574A (ja) 1995-12-01 1997-06-17 Toyobo Co Ltd 新規なクレアチニンアミドヒドロラーゼ
JP2006303451A (ja) 2005-03-23 2006-11-02 Renesas Technology Corp 半導体装置及び半導体装置の製造方法
JP2008136477A (ja) * 2006-04-25 2008-06-19 Toyobo Co Ltd 基質との親和性が向上したクレアチニンアミドヒドロラーゼ改変体、および、クレアチニン測定用試薬組成物

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS51115989A (en) 1975-04-05 1976-10-13 Noda Sangyo Kagaku Kenkyusho Process for preparing creatinine amide hydrolase
JP2527035B2 (ja) 1989-06-16 1996-08-21 東洋紡績株式会社 クレアチニン・アミドヒドロラ―ゼをコ―ドする遺伝子を含有するｄｎａ断片、該ｄｎａ断片を有する組換えベクタ―及び該組換えベクタ―を有する形質転換体、並びにクレアチニン・アミドヒドロラ―ゼの製造方法
JPH09154574A (ja) 1995-12-01 1997-06-17 Toyobo Co Ltd 新規なクレアチニンアミドヒドロラーゼ
JP2006303451A (ja) 2005-03-23 2006-11-02 Renesas Technology Corp 半導体装置及び半導体装置の製造方法
JP2008136477A (ja) * 2006-04-25 2008-06-19 Toyobo Co Ltd 基質との親和性が向上したクレアチニンアミドヒドロラーゼ改変体、および、クレアチニン測定用試薬組成物

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BEUTH, B. ET AL.: "Crystal structure of creatininase from Pseudomonas putida: a novel fold and a case of convergent evolution.", J MOL BIOL., vol. 332, 2003, pages 287 - 301, XP002605326 *
BIOSCI. BIOTECH. BIOCHEM., vol. 59, no. 7, 1995, pages 1331 - 1332
CHEMICAL AND PHARMACEUTICAL BULLETIN, vol. 34, no. 1, 1986, pages 269 - 274
JOURNAL OF BIOCHEMISTRY, vol. 86, 1979, pages 1109 - 1117
JOURNAL OF MOLECULAR BIOLOGY, vol. 332, 2004, pages 287 - 301
JOURNAL OF MOLECULAR BIOLOGY, vol. 337, 2004, pages 399 - 416
SCIENCE, vol. 214, 1981, pages 1205
See also references of EP2202304A4 *

Also Published As

Publication number Publication date
EP2202304A4 (en) 2010-11-24
EP2202304A1 (en) 2010-06-30
EP2202304B1 (en) 2016-08-17

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