WO2009035770A3 - Cholera toxin subunit b conjugated quantum dots for live cell labelling - Google Patents

Cholera toxin subunit b conjugated quantum dots for live cell labelling Download PDF

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Publication number
WO2009035770A3
WO2009035770A3 PCT/US2008/070872 US2008070872W WO2009035770A3 WO 2009035770 A3 WO2009035770 A3 WO 2009035770A3 US 2008070872 W US2008070872 W US 2008070872W WO 2009035770 A3 WO2009035770 A3 WO 2009035770A3
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WO
WIPO (PCT)
Prior art keywords
labelling
conjugates
quantum dots
cholera toxin
live cell
Prior art date
Application number
PCT/US2008/070872
Other languages
French (fr)
Other versions
WO2009035770A2 (en
Inventor
Byron Ballou
Marcel P Bruchez
Subhasish K Chakraborty
James A J Fitzpatrick
Susan Andreko
Justin C Crowley
Original Assignee
Univ Carnegie Mellon
Byron Ballou
Marcel P Bruchez
Subhasish K Chakraborty
James A J Fitzpatrick
Susan Andreko
Justin C Crowley
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Carnegie Mellon, Byron Ballou, Marcel P Bruchez, Subhasish K Chakraborty, James A J Fitzpatrick, Susan Andreko, Justin C Crowley filed Critical Univ Carnegie Mellon
Publication of WO2009035770A2 publication Critical patent/WO2009035770A2/en
Publication of WO2009035770A3 publication Critical patent/WO2009035770A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/28—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Vibrionaceae (F)

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Nanotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Materials Engineering (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medical Informatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Conjugates were developed for internal labelling and tracking of live cells having cell surface gangliosides. The conjugate is comprised of at least one, and preferably up to three Vibrio cholerae toxin subunits B, preferably in the pentameric form, attached to a quantum dot. The conjugates may be used as luminescent labels in a reagent system for labelling live cells. The conjugates are internalized into small vesicles dispersed throughout the cytoplasm. A method of labelling and method of detecting cells having cell surface gangliosides are described. The conjugates are particularly useful for the general labelling of mammalian cells.
PCT/US2008/070872 2007-07-23 2008-07-23 Cholera toxin subunit b conjugated quantum dots for live cell labelling WO2009035770A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US96163507P 2007-07-23 2007-07-23
US60/961,635 2007-07-23

Publications (2)

Publication Number Publication Date
WO2009035770A2 WO2009035770A2 (en) 2009-03-19
WO2009035770A3 true WO2009035770A3 (en) 2009-05-14

Family

ID=40382083

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/070872 WO2009035770A2 (en) 2007-07-23 2008-07-23 Cholera toxin subunit b conjugated quantum dots for live cell labelling

Country Status (1)

Country Link
WO (1) WO2009035770A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2403400T3 (en) 2009-03-04 2017-01-30 Sunstar Suisse Sa Apparatus for detecting bruxism
KR20140106550A (en) * 2011-11-30 2014-09-03 에이전시 포 사이언스, 테크놀로지 앤드 리서치 GM1 ganglioside to Annexin V microparticle polypeptide ratio for biological monitoring
CN109293754A (en) * 2018-09-29 2019-02-01 郑州大学第附属医院 Novel red fluorescence nano Neural tracer

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
CHAKRABORTY SUBHASISH K ET AL: "Cholera toxin B conjugated quantum dots for live cell labeling", NANO LETTERS, ACS, WASHINGTON, DC, US, vol. 7, no. 9, 31 July 2007 (2007-07-31), pages 2618 - 2626, XP009102072, ISSN: 1530-6984 *
DERFUS A M ET AL: "Intracellular delivery of quantum dots for live cell labeling and organelle tracking", ADVANCED MATERIALS 20040617 WILEY-VCH VERLAG DE, vol. 16, no. 12, 17 June 2004 (2004-06-17), pages 961 - 966, XP002518316 *
FUJINAGA YUKAKO ET AL: "Gangliosides that associate with lipid rafts mediate transport of cholera and related toxins from the plasma membrane to endoplasmic reticulm.", MOLECULAR BIOLOGY OF THE CELL DEC 2003, vol. 14, no. 12, December 2003 (2003-12-01), pages 4783 - 4793, XP002518312, ISSN: 1059-1524 *
HOTZ CHARLES Z ED - ROSENTHAL SANDRA J ET AL: "Applications of quantum dots in biology: an overview", NANOBIOTECHNOLOGY PROTOCOLS, HUMANA PRESS, UNITED STATES, vol. 303, 1 January 2005 (2005-01-01), pages 1 - 17, XP008103243, ISBN: 978-1-58829-276-6 *
JAISWAL J K ET AL: "USE OF QUANTUM DOTS FOR LIVE CELL IMAGING", NATURE METHODS, NATURE PUBLISHING GROUP, GB, vol. 1, no. 1, 1 October 2004 (2004-10-01), pages 73 - 78, XP008062140, ISSN: 1548-7091 *
JYOTI K JAISWAL AND SANFORD M SIMON: "Optical Monitoring of Single Cells Using Quantum Dots", METHODS IN MOLECULAR BIOLOGY, vol. 374, January 2007 (2007-01-01), pages 93 - 104, XP008103244 *
LENCER W I ET AL: "The intracellular voyage of cholera toxin: going retro", TRENDS IN BIOCHEMICAL SCIENCES, ELSEVIER, HAYWARDS, GB, vol. 28, no. 12, 1 December 2003 (2003-12-01), pages 639 - 645, XP004476602, ISSN: 0968-0004 *
MICHALET X ET AL: "QUANTUM DOTS FOR LIVE CELLS IN VIVO IMAGING AND DIAGNOSTICS", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, WASHINGTON, DC, vol. 307, no. 5709, 28 January 2005 (2005-01-28), pages 538 - 544, XP001222940, ISSN: 0036-8075 *
SUGIMOTO Y ET AL: "Accumulation of cholera toxin and GM1 ganglioside in the early endosome of Niemann-Pick C1-deficient cells.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 23 OCT 2001, vol. 98, no. 22, 23 October 2001 (2001-10-23), pages 12391 - 12396, XP002518311, ISSN: 0027-8424 *
VAN TILBORG GERALDA A F ET AL: "Annexin A5-conjugated quantum dots with a paramagnetic lipidic coating for the multimodal detection of apoptotic cells.", BIOCONJUGATE CHEMISTRY 2006 JUL-AUG, vol. 17, no. 4, July 2006 (2006-07-01), pages 865 - 868, XP002518313, ISSN: 1043-1802 *

Also Published As

Publication number Publication date
WO2009035770A2 (en) 2009-03-19

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