WO2009035554A3 - Superior structure stability and selectivity of hairpin nucleic acid probes with an l-dna stem - Google Patents
Superior structure stability and selectivity of hairpin nucleic acid probes with an l-dna stem Download PDFInfo
- Publication number
- WO2009035554A3 WO2009035554A3 PCT/US2008/010466 US2008010466W WO2009035554A3 WO 2009035554 A3 WO2009035554 A3 WO 2009035554A3 US 2008010466 W US2008010466 W US 2008010466W WO 2009035554 A3 WO2009035554 A3 WO 2009035554A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- dna
- selectivity
- acid probes
- stem
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Hairpin-shaped nucleic acid probes capable of forming stable hybrids with target nucleic acid sequences are provided. In particular, an oligonucleotide probe for hybridization analysis is provided, wherein the probe comprises a non-natural enantiomeric DNA (L-DNA) that forms the stem and natural DNA (D-DNA) that forms the loop in the hairpin. The invention thereby provides stable probes that experience fewer stem invasions and demonstrate sensitive and selective hybridization to target nucleic acid sequences. The subject probes are useful for a variety of biological and biotechnological applications.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US96789407P | 2007-09-07 | 2007-09-07 | |
US60/967,894 | 2007-09-07 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009035554A2 WO2009035554A2 (en) | 2009-03-19 |
WO2009035554A3 true WO2009035554A3 (en) | 2009-05-14 |
Family
ID=40452748
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2008/010466 WO2009035554A2 (en) | 2007-09-07 | 2008-09-08 | Superior structure stability and selectivity of hairpin nucleic acid probes with an l-dna stem |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2009035554A2 (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB201021873D0 (en) * | 2010-12-23 | 2011-02-02 | Mologen Ag | DNA expression construct |
GB201021867D0 (en) * | 2010-12-23 | 2011-02-02 | Mologen Ag | Non-coding immunomodulatory DNA construct |
LU92821B1 (en) | 2015-09-09 | 2017-03-20 | Mologen Ag | Combination comprising immunostimulatory oligonucleotides |
GB2542425A (en) | 2015-09-21 | 2017-03-22 | Mologen Ag | Means for the treatment of HIV |
CN116377136B (en) * | 2023-03-14 | 2023-09-15 | 广东海洋大学 | Fluorescent biosensor for rapidly detecting novel crown Omicron strain based on AIE material and application thereof |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020142309A1 (en) * | 2000-07-14 | 2002-10-03 | Nanibhushan Dattagupta | Nucleic acid hairpin probes and uses thereof |
-
2008
- 2008-09-08 WO PCT/US2008/010466 patent/WO2009035554A2/en active Application Filing
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020142309A1 (en) * | 2000-07-14 | 2002-10-03 | Nanibhushan Dattagupta | Nucleic acid hairpin probes and uses thereof |
Non-Patent Citations (3)
Title |
---|
BRENT C. SATTERFIELD ET AL.: "Tentacle probes: eliminating false positives without sacrificing sensitivity.", NUCLEIC ACIDS RESEARCH, vol. 35, no. 10, 21 May 2007 (2007-05-21), pages E77 * |
CAROLINE CREY-DESBIOLLES ET AL.: "Molecular beacons with a homo-DNA stem: improving target selectivity", NUCLEIC ACIDS RESEARCH, vol. 33, no. 8, 5 May 2005 (2005-05-05), pages E77 * |
YOUNGMI KIM ET AL.: "Superior structure stability and selectivity of hairpin nucleic acid probes with an L-DNA stem.", NUCLEIC ACIDS RESEARCH, vol. 35, no. 21, 24 October 2007 (2007-10-24), pages 7279 - 7287 * |
Also Published As
Publication number | Publication date |
---|---|
WO2009035554A2 (en) | 2009-03-19 |
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