WO2009035554A3 - Superior structure stability and selectivity of hairpin nucleic acid probes with an l-dna stem - Google Patents

Superior structure stability and selectivity of hairpin nucleic acid probes with an l-dna stem Download PDF

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Publication number
WO2009035554A3
WO2009035554A3 PCT/US2008/010466 US2008010466W WO2009035554A3 WO 2009035554 A3 WO2009035554 A3 WO 2009035554A3 US 2008010466 W US2008010466 W US 2008010466W WO 2009035554 A3 WO2009035554 A3 WO 2009035554A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
dna
selectivity
acid probes
stem
Prior art date
Application number
PCT/US2008/010466
Other languages
French (fr)
Other versions
WO2009035554A2 (en
Inventor
Weihong Tan
Youngmi Kim
Chaoyong James Yang
Original Assignee
Univ Florida
Weihong Tan
Youngmi Kim
Chaoyong James Yang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Florida, Weihong Tan, Youngmi Kim, Chaoyong James Yang filed Critical Univ Florida
Publication of WO2009035554A2 publication Critical patent/WO2009035554A2/en
Publication of WO2009035554A3 publication Critical patent/WO2009035554A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Hairpin-shaped nucleic acid probes capable of forming stable hybrids with target nucleic acid sequences are provided. In particular, an oligonucleotide probe for hybridization analysis is provided, wherein the probe comprises a non-natural enantiomeric DNA (L-DNA) that forms the stem and natural DNA (D-DNA) that forms the loop in the hairpin. The invention thereby provides stable probes that experience fewer stem invasions and demonstrate sensitive and selective hybridization to target nucleic acid sequences. The subject probes are useful for a variety of biological and biotechnological applications.
PCT/US2008/010466 2007-09-07 2008-09-08 Superior structure stability and selectivity of hairpin nucleic acid probes with an l-dna stem WO2009035554A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US96789407P 2007-09-07 2007-09-07
US60/967,894 2007-09-07

Publications (2)

Publication Number Publication Date
WO2009035554A2 WO2009035554A2 (en) 2009-03-19
WO2009035554A3 true WO2009035554A3 (en) 2009-05-14

Family

ID=40452748

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/010466 WO2009035554A2 (en) 2007-09-07 2008-09-08 Superior structure stability and selectivity of hairpin nucleic acid probes with an l-dna stem

Country Status (1)

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WO (1) WO2009035554A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201021873D0 (en) * 2010-12-23 2011-02-02 Mologen Ag DNA expression construct
GB201021867D0 (en) * 2010-12-23 2011-02-02 Mologen Ag Non-coding immunomodulatory DNA construct
LU92821B1 (en) 2015-09-09 2017-03-20 Mologen Ag Combination comprising immunostimulatory oligonucleotides
GB2542425A (en) 2015-09-21 2017-03-22 Mologen Ag Means for the treatment of HIV
CN116377136B (en) * 2023-03-14 2023-09-15 广东海洋大学 Fluorescent biosensor for rapidly detecting novel crown Omicron strain based on AIE material and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020142309A1 (en) * 2000-07-14 2002-10-03 Nanibhushan Dattagupta Nucleic acid hairpin probes and uses thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020142309A1 (en) * 2000-07-14 2002-10-03 Nanibhushan Dattagupta Nucleic acid hairpin probes and uses thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BRENT C. SATTERFIELD ET AL.: "Tentacle probes: eliminating false positives without sacrificing sensitivity.", NUCLEIC ACIDS RESEARCH, vol. 35, no. 10, 21 May 2007 (2007-05-21), pages E77 *
CAROLINE CREY-DESBIOLLES ET AL.: "Molecular beacons with a homo-DNA stem: improving target selectivity", NUCLEIC ACIDS RESEARCH, vol. 33, no. 8, 5 May 2005 (2005-05-05), pages E77 *
YOUNGMI KIM ET AL.: "Superior structure stability and selectivity of hairpin nucleic acid probes with an L-DNA stem.", NUCLEIC ACIDS RESEARCH, vol. 35, no. 21, 24 October 2007 (2007-10-24), pages 7279 - 7287 *

Also Published As

Publication number Publication date
WO2009035554A2 (en) 2009-03-19

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