WO2009031671A1 - ｐ５３の発現促進方法およびそれに用いるｐ５３発現促進剤 - Google Patents

ｐ５３の発現促進方法およびそれに用いるｐ５３発現促進剤 Download PDF

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Publication number
WO2009031671A1
WO2009031671A1 PCT/JP2008/066127 JP2008066127W WO2009031671A1 WO 2009031671 A1 WO2009031671 A1 WO 2009031671A1 JP 2008066127 W JP2008066127 W JP 2008066127W WO 2009031671 A1 WO2009031671 A1 WO 2009031671A1
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WO
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Prior art keywords
expression
gene
transcribed
exon
vegf
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PCT/JP2008/066127
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English (en)
French (fr)
Inventor
Shigetada Kondo
Kazuhito Rokutan
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The University Of Tokushima
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Publication date
Application filed by The University Of Tokushima filed Critical The University Of Tokushima
Priority to US12/676,443 priority Critical patent/US20100197765A1/en
Priority to CN200880106106.2A priority patent/CN101795714B/zh
Priority to EP08829249A priority patent/EP2198891A4/en
Priority to JP2009531301A priority patent/JP5273740B2/ja
Priority to CA2697737A priority patent/CA2697737A1/en
Publication of WO2009031671A1 publication Critical patent/WO2009031671A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1136—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against growth factors, growth regulators, cytokines, lymphokines or hormones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/337—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having four-membered rings, e.g. taxol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7105—Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/711—Natural deoxyribonucleic acids, i.e. containing only 2'-deoxyriboses attached to adenine, guanine, cytosine or thymine and having 3'-5' phosphodiester links
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Genetics & Genomics (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Epidemiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Endocrinology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　ｐ５３ｍＲＮＡの発現を促進する方法およびそれに用いる発現促進剤を提供する。 　ｖｅｇｆ－Ａ遺伝子のエキソン１から転写されるＲＮＡと、ｐ５３遺伝子から転写されるｍＲＮＡとの結合を阻害することにより、ｐ５３遺伝子の発現を促進する。前記結合の阻害は、例えば、ｖｅｇｆ－Ａ遺伝子のエキソン１から転写されたＲＮＡの分解、ｖｅｇｆ－Ａ遺伝子のエキソン１からのＲＮＡの転写阻害、または、ｖｅｇｆ－Ａ遺伝子のエキソン１から転写されたＲＮＡに、ｐ５３遺伝子から転写されたｍＲＮＡとは別の核酸を結合させることで実現できる。
PCT/JP2008/066127 2007-09-07 2008-09-05 ｐ５３の発現促進方法およびそれに用いるｐ５３発現促進剤 WO2009031671A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US12/676,443 US20100197765A1 (en) 2007-09-07 2008-09-05 Method for promoting the expression of p53, and p53 expression promoter for use in the method
CN200880106106.2A CN101795714B (zh) 2007-09-07 2008-09-05 p53的表达促进方法和该方法中使用的p53表达促进剂
EP08829249A EP2198891A4 (en) 2007-09-07 2008-09-05 METHOD OF PROMOTING EXPRESSION OF P53 AND P53 EXPRESSION PROMOTERS FOR USE IN THE METHOD
JP2009531301A JP5273740B2 (ja) 2007-09-07 2008-09-05 ｐ５３の発現促進方法およびそれに用いるｐ５３発現促進剤
CA2697737A CA2697737A1 (en) 2007-09-07 2008-09-05 Method for promoting expression of p53, and p53 expression promoter for use in the method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007233316 2007-09-07
JP2007-233316 2007-09-07

Publications (1)

Publication Number Publication Date
WO2009031671A1 true WO2009031671A1 (ja) 2009-03-12

Family

ID=40428983

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/066127 WO2009031671A1 (ja) 2007-09-07 2008-09-05 ｐ５３の発現促進方法およびそれに用いるｐ５３発現促進剤

Country Status (6)

Country Link
US (1) US20100197765A1 (ja)
EP (1) EP2198891A4 (ja)
JP (1) JP5273740B2 (ja)
CN (1) CN101795714B (ja)
CA (1) CA2697737A1 (ja)
WO (1) WO2009031671A1 (ja)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1252308A2 (en) * 2000-01-19 2002-10-30 Parkash S. Gill Pharmaceutical compositions and methods of treatment based on vegf antisense oligonucleotides
US20050148530A1 (en) * 2002-02-20 2005-07-07 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US20050054596A1 (en) * 2001-11-30 2005-03-10 Mcswiggen James RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
FR2832154B1 (fr) * 2001-11-09 2007-03-16 Centre Nat Rech Scient Oligonucleotides inhibiteurs et leur utilisation pour reprimer specifiquement un gene
US8426194B2 (en) * 2003-01-21 2013-04-23 Ptc Therapeutics, Inc. Methods and agents for screening for compounds capable of modulating VEGF expression
CN100352922C (zh) * 2003-08-07 2007-12-05 徐根兴 抑制人VEGF基因的表达的siRNA和表达载体及其在制药中的应用
EP2899278A1 (en) * 2004-03-12 2015-07-29 Alnylam Pharmaceuticals Inc. iRNA agents targeting VEGF
US20060134661A1 (en) * 2004-10-12 2006-06-22 Richard Essner Treatment of cancer and compositions
MX2007012766A (es) * 2005-04-12 2008-10-01 Intradigm Corp Composición y métodos de terapéuticos de arni para el tratamiento de cáncer y otras enfermedades de neovascularización.
CN100371444C (zh) * 2005-12-12 2008-02-27 清华大学深圳研究生院 一种抑制VEGF表达的siRNA及其应用

Non-Patent Citations (23)

* Cited by examiner, † Cited by third party
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BIROCCIO, A. ET AL.: "The future of antisense therapy: combination with anticancer treatments", ONCOGENE, vol. 22, no. 42, 2003, pages 6579 - 88, XP008118435 *
BORNES, S. ET AL., J. BIOL. CHEM., vol. 279, 2004, pages 18717 - 18726
BUNZ, F. ET AL., J. CLIN. INVEST., vol. 104, 1999, pages 263 - 269
HASSAN, I. ET AL.: "Antisense p53 decreases production of VEGF in follicular thyroid cancer cells", ENDOCRINE, vol. 29, no. 3, 2006, pages 409 - 12, XP008132342 *
HIRASHIMA; INOUE: "Idenshi no Fukusei to Hatsugen", 1993, JAPANESE BIOCHEMICAL SOCIETY, article "Shin Seikagaku Jikken Koza 2", pages: 319 - 347
HUEZ, 1. ET AL., MOL. CELL. BIOL., vol. 18, 1998, pages 6178 - 6190
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Also Published As

Publication number Publication date
CA2697737A1 (en) 2009-03-12
CN101795714A (zh) 2010-08-04
CN101795714B (zh) 2014-05-14
EP2198891A4 (en) 2011-12-21
JPWO2009031671A1 (ja) 2010-12-16
EP2198891A1 (en) 2010-06-23
JP5273740B2 (ja) 2013-08-28
US20100197765A1 (en) 2010-08-05

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