WO2009030720A3 - Process for producing a recombinant protein - Google Patents

Process for producing a recombinant protein Download PDF

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Publication number
WO2009030720A3
WO2009030720A3 PCT/EP2008/061672 EP2008061672W WO2009030720A3 WO 2009030720 A3 WO2009030720 A3 WO 2009030720A3 EP 2008061672 W EP2008061672 W EP 2008061672W WO 2009030720 A3 WO2009030720 A3 WO 2009030720A3
Authority
WO
WIPO (PCT)
Prior art keywords
producing
recombinant protein
transferrin
insulin
synergistic
Prior art date
Application number
PCT/EP2008/061672
Other languages
French (fr)
Other versions
WO2009030720A2 (en
Inventor
Larissa Chirkova
Catherine Anne Yandell
Original Assignee
Novozymes Biopharma Dk As
Larissa Chirkova
Catherine Anne Yandell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Biopharma Dk As, Larissa Chirkova, Catherine Anne Yandell filed Critical Novozymes Biopharma Dk As
Publication of WO2009030720A2 publication Critical patent/WO2009030720A2/en
Publication of WO2009030720A3 publication Critical patent/WO2009030720A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2839—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily
    • C07K16/2845—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily against integrin beta2-subunit-containing molecules, e.g. CD11, CD18
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Abstract

The addition of an insulin-like factor and transferrin to serum-free media is synergistic in increasing the expression of recombinant proteins in mammalian cells.
PCT/EP2008/061672 2007-09-06 2008-09-04 Process for producing a recombinant protein WO2009030720A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07115833.1 2007-09-06
EP07115833 2007-09-06

Publications (2)

Publication Number Publication Date
WO2009030720A2 WO2009030720A2 (en) 2009-03-12
WO2009030720A3 true WO2009030720A3 (en) 2009-04-30

Family

ID=40329101

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/061672 WO2009030720A2 (en) 2007-09-06 2008-09-04 Process for producing a recombinant protein

Country Status (1)

Country Link
WO (1) WO2009030720A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010115634A1 (en) * 2009-04-09 2010-10-14 Cellca Gmbh Method for improved single cell cloning
US9238080B2 (en) 2010-05-21 2016-01-19 Merrimack Pharmaceuticals, Inc. Bi-specific fusion proteins
WO2012023085A1 (en) * 2010-08-20 2012-02-23 Wyeth Llc Cell culture of growth factor-free adapted cells
US9675671B2 (en) * 2014-01-12 2017-06-13 Igf Oncology, Llc Fusion proteins containing insulin-like growth factor-1 and epidermal growth factor and variants thereof and uses thereof
EP3355907B1 (en) 2015-10-02 2021-01-20 Silver Creek Pharmaceuticals, Inc. Bi-specific therapeutic proteins for tissue repair

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0325190A2 (en) * 1988-01-18 1989-07-26 Roche Diagnostics GmbH Pentosansulfate medium
WO2005070120A2 (en) * 2004-01-09 2005-08-04 Serologicals Investment Company, Inc. Cell culture media
WO2007068053A1 (en) * 2005-12-15 2007-06-21 Gropep Limited Recombinant protein production
WO2008009641A1 (en) * 2006-07-17 2008-01-24 Novozymes A/S Cell culture media

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0325190A2 (en) * 1988-01-18 1989-07-26 Roche Diagnostics GmbH Pentosansulfate medium
WO2005070120A2 (en) * 2004-01-09 2005-08-04 Serologicals Investment Company, Inc. Cell culture media
WO2007068053A1 (en) * 2005-12-15 2007-06-21 Gropep Limited Recombinant protein production
WO2008009641A1 (en) * 2006-07-17 2008-01-24 Novozymes A/S Cell culture media

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHUN CHUNG ET AL: "Application of factorial design to accelerate identification of CHO growth factor requirements.", BIOTECHNOLOGY PROGRESS, vol. 19, no. 1, January 2003 (2003-01-01), pages 52 - 57, XP002514491, ISSN: 8756-7938 *
KEENAN JOANNE ET AL: "Evaluation of recombinant human transferrin (DeltaFerrin (TM)) as an iron chelator in serum-free media for mammalian cell culture", CYTOTECHNOLOGY, vol. 51, no. 1, 2006, pages 29 - 37, XP002514493, ISSN: 0920-9069 *
P. CONEN: "Development of cost effective media formulation for rCHO cell lines by eliminating or reducing serum", DIPLOMA THESIS, April 2006 (2006-04-01), XP002514492, Retrieved from the Internet <URL:http://opus.haw-hamburg.de/volltexte/2007/188/pdf/bio_y_187.pdf> *
S. MORTELLARO AND M. DEVINE: "Advances in animal-free manufacturing of Biopharmaceuticals", 2 May 2007 (2007-05-02), pages 1 - 8, XP002514494, Retrieved from the Internet <URL:http://biopharminternational.findpharma.com/biopharm/Article/Advances-in-Animal-Free-Manufacturing-of-Biopharma/ArticleStandard/Article/detail/423195> [retrieved on 20090211] *

Also Published As

Publication number Publication date
WO2009030720A2 (en) 2009-03-12

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