WO2009025290A1 - 神経毒素中和抗体価の定量方法 - Google Patents

神経毒素中和抗体価の定量方法 Download PDF

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Publication number
WO2009025290A1
WO2009025290A1 PCT/JP2008/064813 JP2008064813W WO2009025290A1 WO 2009025290 A1 WO2009025290 A1 WO 2009025290A1 JP 2008064813 W JP2008064813 W JP 2008064813W WO 2009025290 A1 WO2009025290 A1 WO 2009025290A1
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WO
WIPO (PCT)
Prior art keywords
neurotoxin
neutralizing antibody
titer
quantifying
antibody against
Prior art date
Application number
PCT/JP2008/064813
Other languages
English (en)
French (fr)
Inventor
Yasushi Torii
Tetsuhiro Harakawa
Yoshitaka Goto
Akihiro Ginnaga
Ryuji Kaji
Shunji Kozaki
Motohide Takahashi
Original Assignee
Juridical Foundation The Chemo-Sero-Therapeutic Research Institute
Japan As Represented By Director General Of National Institute Of Infectious Diseases
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Juridical Foundation The Chemo-Sero-Therapeutic Research Institute, Japan As Represented By Director General Of National Institute Of Infectious Diseases filed Critical Juridical Foundation The Chemo-Sero-Therapeutic Research Institute
Priority to US12/673,980 priority Critical patent/US20110105940A1/en
Priority to EP08827849.4A priority patent/EP2189790B1/en
Priority to JP2009529042A priority patent/JPWO2009025290A1/ja
Priority to ES08827849.4T priority patent/ES2585365T3/es
Publication of WO2009025290A1 publication Critical patent/WO2009025290A1/ja

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/24—Detecting, measuring or recording bioelectric or biomagnetic signals of the body or parts thereof
    • A61B5/316—Modalities, i.e. specific diagnostic methods
    • A61B5/389—Electromyography [EMG]
    • A61B5/395—Details of stimulation, e.g. nerve stimulation to elicit EMG response
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/24—Detecting, measuring or recording bioelectric or biomagnetic signals of the body or parts thereof
    • A61B5/316—Modalities, i.e. specific diagnostic methods
    • A61B5/389—Electromyography [EMG]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00—Immunoassays for the detection of microorganisms
    • G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

　神経毒素に対する中和抗体の力価の定量方法を開示する。（ａ）一定量の神経毒素を含む標準試料と当該神経毒素に対する中和抗体を含有する被験試料を混合し、（ｂ）（ａ）の混合物を非ヒト哺乳動物の筋肉に投与し、（ｃ）当該非ヒト哺乳動物に電気刺激をかけ、（ｄ）電気刺激による当該哺乳動物の筋肉収縮に伴う複合筋活動電位（CMAP）を筋電計で測定し、ついで（ｅ）操作ステップ（ｄ）で測定して得られたCMAP振幅データから、未中和の神経毒素による振幅の低下の程度を解析することにより被験試料中に含有される中和抗体の力価を定量化することから構成されることを特徴とする神経毒素に対する中和抗体の力価の定量方法。
PCT/JP2008/064813 2007-08-20 2008-08-20 神経毒素中和抗体価の定量方法 WO2009025290A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US12/673,980 US20110105940A1 (en) 2007-08-20 2008-08-20 Method for quantification of titer of neutralizing antibody to neurotoxin
EP08827849.4A EP2189790B1 (en) 2007-08-20 2008-08-20 Method for quantification of titer of neurotoxin-neutralizing antibody
JP2009529042A JPWO2009025290A1 (ja) 2007-08-20 2008-08-20 神経毒素中和抗体価の定量方法
ES08827849.4T ES2585365T3 (es) 2007-08-20 2008-08-20 Método para la cuantificación del título de un anticuerpo neutralizante de neurotoxinas

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-213889 2007-08-20
JP2007213889 2007-08-20

Publications (1)

Publication Number Publication Date
WO2009025290A1 true WO2009025290A1 (ja) 2009-02-26

Family

ID=40378189

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/064813 WO2009025290A1 (ja) 2007-08-20 2008-08-20 神経毒素中和抗体価の定量方法

Country Status (5)

Country Link
US (1) US20110105940A1 (ja)
EP (1) EP2189790B1 (ja)
JP (1) JPWO2009025290A1 (ja)
ES (1) ES2585365T3 (ja)
WO (1) WO2009025290A1 (ja)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007125604A1 (ja) 2006-04-28 2007-11-08 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute 神経毒素の定量方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007125604A1 (ja) 2006-04-28 2007-11-08 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute 神経毒素の定量方法

Non-Patent Citations (15)

* Cited by examiner, † Cited by third party
Title
DRESSLER ET AL., EUR. NEUROL., vol. 47, 2002, pages 118 - 121
DRESSLER ET AL., EUR. NEUROL., vol. 48, 2002, pages 26 - 29
DUANE ET AL., NEUROLOGY, vol. 48, no. 2, 1997, pages A399
GOSCHEL ET AL., EXP. NEUROL., vol. 147, 1997, pages 96 - 102
HALL, Y.H.J. ET AL.: "Novel application of an in vitro technique to the detection and quantification of botulinum neurotoxin antibodies", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 288, no. 1-2, 2004, pages 55 - 60, XP008131631 *
HALLIS ET AL., J. CLIN. MICROBIOL., vol. 34, 1996, pages 1934 - 1938
HATHEWAY ET AL., J. INFECT. DIS., vol. 150, 1984, pages 407 - 412
MOTOHIDE TAKAHASHI ET AL.: "Fukugokin Katsudo Den'i ni yoru Botulinus Shinkei Dokuso no Kinshikan Kassei no Teiryoka", JAPANESE JOURNAL OF CLINICAL NEUROPHYSIOLOGY, vol. 34, no. 5, 2006, pages 413 *
OSAMU MIMURA ET AL.: "Botulinus ryoho no genjo to shorai no tenbo", BRAIN 21, vol. 5, no. 4, 2002, pages 416 - 419 *
PEARCE, L.B. ET AL.: "Measurement of Botulinum toxin activity", TOXICOL.APPL.PHARMACOL., vol. 128, no. 1, September 1994 (1994-09-01), pages 69 - 77, XP024880303 *
RYUJ I KAJ I ET AL.: "Dystonia and botulinum therapy", SHINDAN-TO-CHIRYOSHA, 2005
SAKAGUCHI, G.; OHISHI, I.; KOZAKI, S.: "BIOCHEMICAL ASPECTS of botulism: Purification and oral toxicities of Clostridium botulinum progenitor toxins", 1981, ACADEMIC PRESS, pages: 21 - 34
See also references of EP2189790A4
SESAARDIC ET AL., MOV. DISORD., vol. 19, no. 8, 2004, pages S85 - S91
SHERIDAN ET AL., J. APPL. TOXICOL., vol. 19, 1999, pages S29 - S33

Also Published As

Publication number Publication date
EP2189790A1 (en) 2010-05-26
US20110105940A1 (en) 2011-05-05
EP2189790B1 (en) 2016-07-20
EP2189790A4 (en) 2010-09-01
JPWO2009025290A1 (ja) 2010-11-25
ES2585365T3 (es) 2016-10-05

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WO2009025290A1 (ja) 神経毒素中和抗体価の定量方法

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