WO2009019012A3 - Immobilisation of chelating groups for immobilised metal ion chromatography (imac) - Google Patents

Immobilisation of chelating groups for immobilised metal ion chromatography (imac) Download PDF

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Publication number
WO2009019012A3
WO2009019012A3 PCT/EP2008/006485 EP2008006485W WO2009019012A3 WO 2009019012 A3 WO2009019012 A3 WO 2009019012A3 EP 2008006485 W EP2008006485 W EP 2008006485W WO 2009019012 A3 WO2009019012 A3 WO 2009019012A3
Authority
WO
WIPO (PCT)
Prior art keywords
immobilisation
imac
metal ion
ion chromatography
chelating groups
Prior art date
Application number
PCT/EP2008/006485
Other languages
French (fr)
Other versions
WO2009019012A2 (en
Inventor
Dirk Goerlich
Steffen Frey
Original Assignee
Max Planck Gesellschaft
Dirk Goerlich
Steffen Frey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=39870668&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2009019012(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from EP07015389A external-priority patent/EP2022561A1/en
Priority to EP08785398.2A priority Critical patent/EP2183049B2/en
Priority to JP2010519377A priority patent/JP5629208B2/en
Priority to US12/672,345 priority patent/US9073971B2/en
Priority to CN2008801057565A priority patent/CN101808731B/en
Application filed by Max Planck Gesellschaft, Dirk Goerlich, Steffen Frey filed Critical Max Planck Gesellschaft
Priority to ES08785398T priority patent/ES2524786T5/en
Priority to EP14173856.7A priority patent/EP2826555B1/en
Priority to CA2695586A priority patent/CA2695586C/en
Publication of WO2009019012A2 publication Critical patent/WO2009019012A2/en
Publication of WO2009019012A3 publication Critical patent/WO2009019012A3/en
Priority to HK10109243.4A priority patent/HK1142843A1/en
Priority to US14/723,770 priority patent/US9675960B2/en
Priority to US15/491,048 priority patent/US10538552B2/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
    • B01J20/10—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate
    • B01J20/103—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate comprising silica
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/24—Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
    • B01J20/28009—Magnetic properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/289—Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3251—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3265—Non-macromolecular compounds with an organic functional group containing a metal, e.g. a metal affinity ligand
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J45/00—Ion-exchange in which a complex or a chelate is formed; Use of material as complex or chelate forming ion-exchangers; Treatment of material for improving the complex or chelate forming ion-exchange properties
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/52—Sorbents specially adapted for preparative chromatography

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention refers to a method for binding a polycarboxylic acid to a solid phase. Further, the invention refers to a solid phase having a polycarboxylic acid immobilized thereto and methods of using the solid phase, e.g. for purifying His-tagged recombinant polypeptides.
PCT/EP2008/006485 2007-08-06 2008-08-06 Immobilisation of chelating groups for immobilised metal ion chromatography (imac) WO2009019012A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
CA2695586A CA2695586C (en) 2007-08-06 2008-08-06 Immobilisation of chelating groups for immobilised metal ion chromatography (imac)
EP14173856.7A EP2826555B1 (en) 2007-08-06 2008-08-06 Use of immobilized chelator for purification of recombinant polypeptides by immobilized metal ion chromatography
JP2010519377A JP5629208B2 (en) 2007-08-06 2008-08-06 Immobilization of chelating groups for immobilized metal ion chromatography (IMAC)
US12/672,345 US9073971B2 (en) 2007-08-06 2008-08-06 Immobilization of chelating groups for immobilized metal ion chromatography (IMAC)
CN2008801057565A CN101808731B (en) 2007-08-06 2008-08-06 Immobilisation of chelating groups for immobilised metal ion chromatography (IMAC)
EP08785398.2A EP2183049B2 (en) 2007-08-06 2008-08-06 Use of immobilized chelator for purification of recombinant proteins by immobilized metal ion chromatography and method of purifying recombinant proteins
ES08785398T ES2524786T5 (en) 2007-08-06 2008-08-06 Use of immobilized chelators for purification of recombinant proteins by immobilized metal ion chromatography and recombinant protein purification method
HK10109243.4A HK1142843A1 (en) 2007-08-06 2010-09-28 Immobilisation of chelating groups for immobilised metal ion chromatography(imac)
US14/723,770 US9675960B2 (en) 2007-08-06 2015-05-28 Immobilization of chelating groups for immobilized metal ion chromatography (IMAC)
US15/491,048 US10538552B2 (en) 2007-08-06 2017-04-19 Immobilisation of chelating groups for immobilised metal ion chromatography (IMAC)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US95414407P 2007-08-06 2007-08-06
EP07015389.5 2007-08-06
US60/954,144 2007-08-06
EP07015389A EP2022561A1 (en) 2007-08-06 2007-08-06 Immobilisation of chelating groups for immobilised metal ion chromatography (IMAC)
EP08012686.5 2008-07-14
EP08012686 2008-07-14

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/672,345 A-371-Of-International US9073971B2 (en) 2007-08-06 2008-08-06 Immobilization of chelating groups for immobilized metal ion chromatography (IMAC)
US14/723,770 Division US9675960B2 (en) 2007-08-06 2015-05-28 Immobilization of chelating groups for immobilized metal ion chromatography (IMAC)

Publications (2)

Publication Number Publication Date
WO2009019012A2 WO2009019012A2 (en) 2009-02-12
WO2009019012A3 true WO2009019012A3 (en) 2009-05-07

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/006485 WO2009019012A2 (en) 2007-08-06 2008-08-06 Immobilisation of chelating groups for immobilised metal ion chromatography (imac)

Country Status (8)

Country Link
US (3) US9073971B2 (en)
EP (2) EP2183049B2 (en)
JP (2) JP5629208B2 (en)
CN (2) CN103586092B (en)
CA (2) CA2934502C (en)
ES (2) ES2832541T3 (en)
HK (2) HK1142843A1 (en)
WO (1) WO2009019012A2 (en)

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CN102161757B (en) * 2011-03-08 2015-07-01 上海树脂厂有限公司 Special resin for vanadium extraction and preparation method thereof
CN103157440B (en) * 2011-12-19 2016-07-06 华东理工大学 The material of heavy metal and method in a kind of adsorption aqueous solution
HU230585B1 (en) * 2012-05-30 2017-01-30 Budapesti Műszaki és Gazdaságtudományi Egyetem Carriers complexed with lanthanide metal ions capable for peptid absorption and isolation
CN104525131A (en) * 2015-01-05 2015-04-22 福州大学 CDTA modified magnetic nano-adsorbent and preparation method and application thereof
ITUA20163876A1 (en) * 2016-05-27 2017-11-27 Univ Del Salento BIORECECTOR FOR METALLIC IONS.
CN106000326B (en) * 2016-06-14 2018-11-02 常州天地人和生物科技有限公司 A kind of super metals tolerant chelates the preparation and application of affine filler
GB201616758D0 (en) * 2016-10-03 2016-11-16 Ge Healthcare Bio Sciences Ab Novel chromatography media
CN107652352B (en) * 2017-10-31 2021-02-26 苏州博进生物技术有限公司 Affinity chromatography medium for purifying histidine-tagged protein
CN109280204A (en) * 2018-08-22 2019-01-29 泉州三欣新材料科技有限公司 A kind of agarose aquogel microballoon and its preparation method and application
EP3887033A1 (en) 2018-11-28 2021-10-06 Cube Biotech GmbH Solid-phase chelator material, method for producing thereof and use thereof for the purification of proteins
CN109663384B (en) * 2018-12-27 2021-05-11 苏州赛分科技有限公司 IMAC (IMAC) filler based on NTA (nitrilotriacetic acid), preparation method and application thereof
CN113646634A (en) * 2019-03-29 2021-11-12 豪夫迈·罗氏有限公司 Transition metal chelate beads
CN109967035A (en) * 2019-04-30 2019-07-05 湘潭大学 A kind of EGTA modified magnetic carbon adsorbent and preparation method thereof and its application method
CN112156499A (en) * 2020-09-22 2021-01-01 上海碧云天生物技术有限公司 Metal chelating chromatography filler and preparation method thereof
CN112812030B (en) * 2020-12-31 2023-05-16 三棵树(上海)新材料研究有限公司 Synthesis method of branched polyamide epoxy curing agent based on ethylenediamine tetraacetic acid
CN113351191B (en) * 2021-05-10 2023-12-01 翌圣生物科技（上海）有限公司 Novel IMAC chromatographic medium of multidentate ligand and preparation method thereof
CN114797804B (en) * 2022-03-29 2023-08-04 翌圣生物科技(上海)股份有限公司 NTA chromatographic medium with long connecting arm and preparation method thereof
CN117946306A (en) * 2024-03-26 2024-04-30 江苏海普功能材料有限公司 Diketoxime resin, preparation method thereof and nickel removal method of lithium battery recovery liquid

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CA2934502A1 (en) 2009-02-12
JP5629208B2 (en) 2014-11-19
JP6063907B2 (en) 2017-01-18
ES2524786T5 (en) 2019-06-19
EP2826555A3 (en) 2016-01-27
EP2183049B2 (en) 2019-03-13
EP2183049A2 (en) 2010-05-12
US20110071274A1 (en) 2011-03-24
CN103586092B (en) 2016-06-08
HK1142843A1 (en) 2010-12-17
JP2015028026A (en) 2015-02-12
CA2934502C (en) 2020-03-24
US10538552B2 (en) 2020-01-21
CA2695586A1 (en) 2009-02-12
JP2010535725A (en) 2010-11-25
US9675960B2 (en) 2017-06-13
CN101808731B (en) 2013-10-23
EP2826555B1 (en) 2020-09-23
US9073971B2 (en) 2015-07-07
EP2826555A2 (en) 2015-01-21
US20150283536A1 (en) 2015-10-08
EP2183049B1 (en) 2014-09-17
CA2695586C (en) 2016-09-06
US20170218013A1 (en) 2017-08-03
ES2832541T3 (en) 2021-06-10
HK1190978A1 (en) 2014-07-18
WO2009019012A2 (en) 2009-02-12
CN101808731A (en) 2010-08-18
CN103586092A (en) 2014-02-19
ES2524786T3 (en) 2014-12-12

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