WO2009014340A3 - Process for differentiating embryonic stem cells using porous membrane - Google Patents

Process for differentiating embryonic stem cells using porous membrane Download PDF

Info

Publication number
WO2009014340A3
WO2009014340A3 PCT/KR2008/004159 KR2008004159W WO2009014340A3 WO 2009014340 A3 WO2009014340 A3 WO 2009014340A3 KR 2008004159 W KR2008004159 W KR 2008004159W WO 2009014340 A3 WO2009014340 A3 WO 2009014340A3
Authority
WO
WIPO (PCT)
Prior art keywords
embryonic stem
stem cells
porous membrane
cells
differentiating embryonic
Prior art date
Application number
PCT/KR2008/004159
Other languages
French (fr)
Other versions
WO2009014340A2 (en
Inventor
Soo-Hong Lee
Seong-Eun Ahn
Dae-Hee Lee
Original Assignee
College Medicine Pochon Cha Univ Ind Acad Coop Found
Soo-Hong Lee
Seong-Eun Ahn
Dae-Hee Lee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by College Medicine Pochon Cha Univ Ind Acad Coop Found, Soo-Hong Lee, Seong-Eun Ahn, Dae-Hee Lee filed Critical College Medicine Pochon Cha Univ Ind Acad Coop Found
Publication of WO2009014340A2 publication Critical patent/WO2009014340A2/en
Publication of WO2009014340A3 publication Critical patent/WO2009014340A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/03—Coculture with; Conditioned medium produced by non-embryonic pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1394—Bone marrow stromal cells; whole marrow
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Abstract

The present invention provides a process for differentiating embryonic stem cells by co-culturing embryonic stem cells or an embryoid body derived from embryonic stem cells in a differentiation culture medium comprising feeder cells, wherein the culture medium comprises a porous membrane having the feeder cells attached to a bottom of the porous membrane, and the embryonic stem cells or embryoid body are co-cultured on the porous membrane; and a process for recovering cells differentiated from embryonic stem cells using the same.
PCT/KR2008/004159 2007-07-24 2008-07-16 Process for differentiating embryonic stem cells using porous membrane WO2009014340A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020070073932A KR20090010646A (en) 2007-07-24 2007-07-24 Process for differentiating embryonic stem cells using porous membrane
KR10-2007-0073932 2007-07-24

Publications (2)

Publication Number Publication Date
WO2009014340A2 WO2009014340A2 (en) 2009-01-29
WO2009014340A3 true WO2009014340A3 (en) 2009-03-19

Family

ID=40281958

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2008/004159 WO2009014340A2 (en) 2007-07-24 2008-07-16 Process for differentiating embryonic stem cells using porous membrane

Country Status (2)

Country Link
KR (1) KR20090010646A (en)
WO (1) WO2009014340A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20110048718A (en) * 2009-11-03 2011-05-12 차의과학대학교 산학협력단 A method for culturing human embryonic stem cells or induced pluripotent stem cells
US20230051601A1 (en) * 2019-12-16 2023-02-16 Kangstem Biotech Co., Ltd. Method for producing stem cell with enhanced efficacy

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050233448A1 (en) * 2003-06-18 2005-10-20 Oh Steve K W Materials and methods to produce stem cells
US20080003676A1 (en) * 2006-06-26 2008-01-03 Millipore Corporation Growth of embryonic stem cells
KR20080016000A (en) * 2006-08-17 2008-02-21 박현숙 Method for co-culture of stem cells and feeder cells using a polymer membrane

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050233448A1 (en) * 2003-06-18 2005-10-20 Oh Steve K W Materials and methods to produce stem cells
US20080003676A1 (en) * 2006-06-26 2008-01-03 Millipore Corporation Growth of embryonic stem cells
KR20080016000A (en) * 2006-08-17 2008-02-21 박현숙 Method for co-culture of stem cells and feeder cells using a polymer membrane

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KIM,S.ET AL.: "A novel culture technique for human embryonic stem cells using porous membranes", STEM CELLS (FIRST PUBLISHED ONLINE IN STEM CELLS EXPRESS), vol. 25, no. 10, 12 July 2007 (2007-07-12), pages 2601 - 2609 *

Also Published As

Publication number Publication date
WO2009014340A2 (en) 2009-01-29
KR20090010646A (en) 2009-01-30

Similar Documents

Publication Publication Date Title
WO2007026353A3 (en) Media for culturing stem cells
WO2008129563A3 (en) Human mesenchymal stem cells and preparation thereof
EP2896690A3 (en) Use of RNA for reprogramming somatic cells
GB2449772A (en) Large scale production of stem cells
NZ567334A (en) Production of oligodendrocytes from placenta-derived stem cells
WO2007146123A3 (en) Placental niche and use thereof to culture stem cells
WO2010129294A3 (en) Small molecules supporting pluripotent cell growth and methods thereof
WO2011056416A3 (en) Cardiomyocyte production
WO2010033906A3 (en) Efficient induction of pluripotent stem cells using small molecule compounds
WO2006070370A3 (en) Stem cells culture systems
WO2010048417A3 (en) Perfusion bioreactors, cell culture systems, and methods for production of cells and cell-derived products
GB2454386A (en) Method for stem cell culture and cells derived therefrom
EP4223769A3 (en) Nuclear reprogramming factor
WO2009061442A8 (en) Method to produce induced pluripotent stem (ips) cells form non-embryonic human cells
NZ592622A (en) Stem cell aggregate suspension compositions and methods for differentiation thereof
WO2007143117A3 (en) Differentiation of primate pluripotent cells to hepatocyte-lineage cells
WO2007127698A3 (en) Adipose derived adult stem cells in hepatic regeneration
IL177493A (en) Use of a serum-free cell culture medium for the production of il-18bp
WO2010129441A3 (en) Method for separating stem cells from their more differentiated progeny using microfluidic devices
WO2008156708A3 (en) Methods and compositions for enhanced differentiation from embryonic stem cells
WO2010020876A3 (en) Cell culture method to form aggregates
WO2006004728A3 (en) Cell culture medium comprising transition metals or trace elements
WO2011116922A3 (en) Thermoreactive substrate with microgels, method for its preparation, and culture method for biological cells
WO2006130504A3 (en) Methods for identifying ligands for stem cells and cells derived therefrom
WO2007022348A3 (en) Amplification of cell polulations from embryonic stem cells

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08778816

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 08778816

Country of ref document: EP

Kind code of ref document: A2