WO2009004142A8 - Procede de fixation sur un microsysteme de composes a liaisons peptidiques, tels que des proteines, et microsysteme incorporant ces composes - Google Patents

Procede de fixation sur un microsysteme de composes a liaisons peptidiques, tels que des proteines, et microsysteme incorporant ces composes Download PDF

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Publication number
WO2009004142A8
WO2009004142A8 PCT/FR2008/000704 FR2008000704W WO2009004142A8 WO 2009004142 A8 WO2009004142 A8 WO 2009004142A8 FR 2008000704 W FR2008000704 W FR 2008000704W WO 2009004142 A8 WO2009004142 A8 WO 2009004142A8
Authority
WO
WIPO (PCT)
Prior art keywords
microsystem
compounds
fixing
proteins
incorporating
Prior art date
Application number
PCT/FR2008/000704
Other languages
English (en)
Other versions
WO2009004142A2 (fr
WO2009004142A3 (fr
Inventor
Frederic Revol-Cavalier
Marc Block
Herve Guillou
Original Assignee
Commissariat Energie Atomique
Centre Nat Rech Scient
Univ Joseph Fourier
Frederic Revol-Cavalier
Marc Block
Herve Guillou
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commissariat Energie Atomique, Centre Nat Rech Scient, Univ Joseph Fourier, Frederic Revol-Cavalier, Marc Block, Herve Guillou filed Critical Commissariat Energie Atomique
Publication of WO2009004142A2 publication Critical patent/WO2009004142A2/fr
Publication of WO2009004142A8 publication Critical patent/WO2009004142A8/fr
Publication of WO2009004142A3 publication Critical patent/WO2009004142A3/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00653—Making arrays on substantially continuous surfaces the compounds being bound to electrodes embedded in or on the solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00709—Type of synthesis
    • B01J2219/00713—Electrochemical synthesis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00725—Peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un procédé de fixation d'un ou plusieurs composé(s) à liaisons peptidiques, tels que des protéines, sur un microsystème de type comportant un réseau d'électrodes recouvrant un substrat, et un tel microsystème incorporant ces composés. Le procédé de fixation selon l'invention se rapporte à un microsystème (1) comportant un réseau d'électrodes recouvrant un substrat, et il comprend la séquence d'étapes suivantes: a) un dépôt électrolytique sur l'une au moins de ces électrodes d'une couche polymérique à base d'au moins un polymère conducteur électronique hydrophobe, via une mise en contact de la ou des électrode(s) avec un électrolyte (7) comprenant un monomère à polymériser, puis b) une mise en contact de la couche polymérique hydrophobe ainsi obtenue avec un milieu peptidique contenant le(s) dit(s) composé(s) à fixer, pour immobiliser ce(s) dernier(s) sur ledit réseau essentiellement par des liaisons hydrophobes.
PCT/FR2008/000704 2007-05-25 2008-05-22 Procede de fixation sur un microsysteme de composes a liaisons peptidiques, tels que des proteines, et microsysteme incorporant ces composes WO2009004142A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0703722 2007-05-25
FR0703722A FR2916367B1 (fr) 2007-05-25 2007-05-25 Procede de fixation sur un microsysteme de composes a liaisons peptidiques, tels que des proteines, et microsysteme incorporant ces composes.

Publications (3)

Publication Number Publication Date
WO2009004142A2 WO2009004142A2 (fr) 2009-01-08
WO2009004142A8 true WO2009004142A8 (fr) 2009-03-12
WO2009004142A3 WO2009004142A3 (fr) 2009-04-30

Family

ID=38904636

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2008/000704 WO2009004142A2 (fr) 2007-05-25 2008-05-22 Procede de fixation sur un microsysteme de composes a liaisons peptidiques, tels que des proteines, et microsysteme incorporant ces composes

Country Status (2)

Country Link
FR (1) FR2916367B1 (fr)
WO (1) WO2009004142A2 (fr)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5156810A (en) * 1989-06-15 1992-10-20 Biocircuits Corporation Biosensors employing electrical, optical and mechanical signals
CN1192097C (zh) * 1995-03-10 2005-03-09 梅索磅秤技术有限公司 多阵列、多特异性的电化学发光检验
FR2781886B1 (fr) * 1998-07-31 2001-02-16 Commissariat Energie Atomique Micro-systeme a multiple points d'analyse chimique ou biologique
FR2784466B1 (fr) * 1998-10-13 2002-10-18 Commissariat Energie Atomique Micro-systeme a multiple points d'analyse chimique ou biologique mettant en oeuvre un couplage entre les sondes et un substrat
EP1520037A4 (fr) * 2002-02-27 2006-06-07 Miragene Inc Composition chimique a substrat amelioree pour l'immobilisation d'une proteine sur un support rigide
EP1616939A1 (fr) * 2003-03-24 2006-01-18 National Institute for Environmental Studies Milieu de culture cellulaire et preparation solidifiee de proteine ou de peptide d'adhesion cellulaire

Also Published As

Publication number Publication date
WO2009004142A2 (fr) 2009-01-08
FR2916367A1 (fr) 2008-11-28
WO2009004142A3 (fr) 2009-04-30
FR2916367B1 (fr) 2009-07-31

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