WO2009002480A3 - Acyl transferase having altered substrate specificity - Google Patents

Acyl transferase having altered substrate specificity Download PDF

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Publication number
WO2009002480A3
WO2009002480A3 PCT/US2008/007807 US2008007807W WO2009002480A3 WO 2009002480 A3 WO2009002480 A3 WO 2009002480A3 US 2008007807 W US2008007807 W US 2008007807W WO 2009002480 A3 WO2009002480 A3 WO 2009002480A3
Authority
WO
WIPO (PCT)
Prior art keywords
substrate specificity
acyl transferase
altered substrate
perhydrolysis
present
Prior art date
Application number
PCT/US2008/007807
Other languages
French (fr)
Other versions
WO2009002480A2 (en
WO2009002480A9 (en
Inventor
Richard R Bott
Marguerite A Cervin
Original Assignee
Danisco Us Inc Genencor Div
Richard R Bott
Marguerite A Cervin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc Genencor Div, Richard R Bott, Marguerite A Cervin filed Critical Danisco Us Inc Genencor Div
Publication of WO2009002480A2 publication Critical patent/WO2009002480A2/en
Publication of WO2009002480A3 publication Critical patent/WO2009002480A3/en
Publication of WO2009002480A9 publication Critical patent/WO2009002480A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides methods for engineering the M. smegmatis acyltransferase to create compositions comprising at least one enzyme suitable for use in enzymatic aqueous acylation and/or perhydrolysis. The present invention further provides compositions comprising at least one perhydrolase enzyme suitable for use in enzymatic aqueous acylation and/or perhydrolysis.
PCT/US2008/007807 2007-06-26 2008-06-23 Acyl transferase having altered substrate specificity WO2009002480A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US93741707P 2007-06-26 2007-06-26
US60/937,417 2007-06-26

Publications (3)

Publication Number Publication Date
WO2009002480A2 WO2009002480A2 (en) 2008-12-31
WO2009002480A3 true WO2009002480A3 (en) 2009-02-19
WO2009002480A9 WO2009002480A9 (en) 2009-04-09

Family

ID=39950146

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/007807 WO2009002480A2 (en) 2007-06-26 2008-06-23 Acyl transferase having altered substrate specificity

Country Status (1)

Country Link
WO (1) WO2009002480A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4309137B2 (en) 2001-05-18 2009-08-05 ダニスコ エイ／エス Preparation method of dough using enzyme
BRPI0513438A2 (en) 2004-07-16 2011-01-04 Danisco enzymatic method for oil degumming
GB0807161D0 (en) * 2008-04-18 2008-05-21 Danisco Process
US9175234B2 (en) 2008-12-23 2015-11-03 REG Life Sciences, LLC Methods and compositions related to thioesterase enzymes
BR112013000108B1 (en) 2010-07-22 2021-05-11 Unilever Ip Holdings B.V. detergent composition, its uses, and process for cleaning a substrate
US9670513B2 (en) 2011-01-21 2017-06-06 Novozymes A/S Production of fatty acid alkyl esters
MX366939B (en) 2012-04-02 2019-07-31 Reg Life Sciences Llc Improved production of fatty acid derivatives.
EP3359679A1 (en) 2015-10-09 2018-08-15 Novozymes A/S Enzymatic or non-enzymatic biodiesel polishing process
WO2017216382A1 (en) 2016-06-16 2017-12-21 Novozymes A/S Reduction of phospholipids in phospholipid-containing oil material
CN117580956A (en) 2021-05-04 2024-02-20 诺维信公司 Enzymatic treatment of feedstock for Hydrotreated Vegetable Oil (HVO) production
CN115806952B (en) * 2022-09-14 2024-05-03 福建师范大学 Mycobacterium smegmatis acyltransferase mutant capable of being efficiently coupled with glucose oxidase and preparation method and application thereof
CN116855470B (en) * 2023-07-10 2024-06-11 态创生物科技(广州)有限公司 Acyltransferase MsAcT variant, fusion protein, immobilization method and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005056782A2 (en) * 2003-12-03 2005-06-23 Genencor International, Inc. Perhydrolase
WO2007133263A2 (en) * 2005-12-09 2007-11-22 Genencor International, Inc. Acyl transferase useful for decontamination
WO2008019069A2 (en) * 2006-08-03 2008-02-14 Danisco Us, Inc., Genencor Division Enzymatic aqueous acylation
WO2008063400A1 (en) * 2006-11-09 2008-05-29 Danisco Us, Inc., Genencor Division Enzyme for the production of long chain peracid

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005056782A2 (en) * 2003-12-03 2005-06-23 Genencor International, Inc. Perhydrolase
WO2007133263A2 (en) * 2005-12-09 2007-11-22 Genencor International, Inc. Acyl transferase useful for decontamination
WO2008019069A2 (en) * 2006-08-03 2008-02-14 Danisco Us, Inc., Genencor Division Enzymatic aqueous acylation
WO2008063400A1 (en) * 2006-11-09 2008-05-29 Danisco Us, Inc., Genencor Division Enzyme for the production of long chain peracid

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BERNHARDT P ET AL: "MOLECULAR BASIS OF PERHYDROLASE ACTIVITY IN SERINE HYDROLASES", ANGEWANDTE CHEMIE. INTERNATIONAL EDITION, VCH VERLAG, WEINHEIM, DE, vol. 44, no. 18, 29 April 2005 (2005-04-29), pages 2742 - 2746, XP008047813, ISSN: 0570-0833 *
DATABASE Geneseq [online] 24 July 2008 (2008-07-24), "Mycobacterium smegmatis arylesterase SEQ ID 1.", retrieved from EBI accession no. GSP:AQX98615 Database accession no. AQX98615 *
DATABASE Geneseq [online] 24 July 2008 (2008-07-24), "Mycobacterium smegmatis mutant perhydrolase (L12G,F154G).", retrieved from EBI accession no. GSP:ARW66032 Database accession no. ARW66032 *
DATABASE Geneseq [online] 25 August 2005 (2005-08-25), "M. gilvum perhydrolase homolog 14B.", XP002505225, retrieved from EBI accession no. GSP:AEA43714 Database accession no. AEA43714 *
DATABASE Geneseq [online] 25 August 2005 (2005-08-25), "M. parafortuitum perhydrolase homolog 20A.", XP002505226, retrieved from EBI accession no. GSP:AEA43716 Database accession no. AEA43716 *
DATABASE Geneseq [online] 25 August 2005 (2005-08-25), "Perhydrolase homolog SEQ ID NO 695.", retrieved from EBI accession no. GSP:AEA43769 Database accession no. AEA43769 *
MATHEWS IRIMPAN ET AL: "Structure of a novel enzyme that catalyzes acyl transfer to alcohols in aqueous conditions", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, EASTON, PA.; US, vol. 46, no. 31, 1 August 2007 (2007-08-01), pages 8969 - 8979, XP002465298, ISSN: 0006-2960 *

Also Published As

Publication number Publication date
WO2009002480A2 (en) 2008-12-31
WO2009002480A9 (en) 2009-04-09

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