WO2009001135A3 - Combinatorial libraries and related methods - Google Patents

Combinatorial libraries and related methods Download PDF

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Publication number
WO2009001135A3
WO2009001135A3 PCT/GB2008/050495 GB2008050495W WO2009001135A3 WO 2009001135 A3 WO2009001135 A3 WO 2009001135A3 GB 2008050495 W GB2008050495 W GB 2008050495W WO 2009001135 A3 WO2009001135 A3 WO 2009001135A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecular constructs
library
different
tag elements
elements
Prior art date
Application number
PCT/GB2008/050495
Other languages
French (fr)
Other versions
WO2009001135A2 (en
Inventor
Steven Nicholas Kilenyi
Original Assignee
Cythera Technologies Ltd
Steven Nicholas Kilenyi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cythera Technologies Ltd, Steven Nicholas Kilenyi filed Critical Cythera Technologies Ltd
Publication of WO2009001135A2 publication Critical patent/WO2009001135A2/en
Publication of WO2009001135A3 publication Critical patent/WO2009001135A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

There is disclosed a method of synthesizing a library of molecular constructs, comprising a plurality of rounds of synthesis, wherein during at least two of the plurality of rounds of synthesis, tag elements from a group of tag elements are combinatorially attached to molecular constructs to form tags, each of which comprises a plurality of said tag elements, to form a library of more diverse molecular constructs such that, after completion of the said plurality of rounds of synthesis, different molecular constructs within the resulting library have different tags attached thereto, the method further comprising the step of separating molecular constructs from a mixture of the resulting molecular constructs using a plurality of specific binding elements each of which specifically binds a different tag element from the group of different tag elements, or a specific combination of different tag elements from the group of different tag elements, to form a combinatorial library of molecular constructs. There is also disclosed a method of screening a library of molecular constructs in which molecular constructs are separated with spatially separate specific binding regions and then remain spatially separated during screening.
PCT/GB2008/050495 2007-06-25 2008-06-25 Combinatorial libraries and related methods WO2009001135A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0712245A GB0712245D0 (en) 2007-06-25 2007-06-25 Combinatorial libraries and related methods
GB0712245.0 2007-06-25

Publications (2)

Publication Number Publication Date
WO2009001135A2 WO2009001135A2 (en) 2008-12-31
WO2009001135A3 true WO2009001135A3 (en) 2009-03-05

Family

ID=38352842

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2008/050495 WO2009001135A2 (en) 2007-06-25 2008-06-25 Combinatorial libraries and related methods

Country Status (2)

Country Link
GB (1) GB0712245D0 (en)
WO (1) WO2009001135A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2336315T3 (en) * 2005-12-01 2017-11-06 Nuevolution As Method for enzymatic coding by efficient synthesis of large libraries
CN107849737B (en) * 2015-07-03 2020-06-26 湖南中晟全肽生化有限公司 Peptide library construction method and related vector
IL266197B2 (en) * 2016-10-24 2024-03-01 Geneinfosec Inc Concealing information present within nucleic acids

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5723598A (en) * 1992-03-30 1998-03-03 The Scripps Research Institute Encoded combinatorial chemical libraries
WO2000023458A1 (en) * 1998-10-19 2000-04-27 The Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library chemistry
WO2002099078A2 (en) * 2001-06-05 2002-12-12 Irm Llc Functional proteomic profiling
WO2004001042A2 (en) * 2002-06-20 2003-12-31 Nuevolution A/S Microarrays displaying encoded molecules
WO2004074501A2 (en) * 2003-02-21 2004-09-02 Nuevolution A/S A method for obtaining structural information about an encoded molecule
WO2006053571A2 (en) * 2004-11-22 2006-05-26 Peter Birk Rasmussen Template directed split and mix systhesis of small molecule libraries

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5723598A (en) * 1992-03-30 1998-03-03 The Scripps Research Institute Encoded combinatorial chemical libraries
US6060596A (en) * 1992-03-30 2000-05-09 The Scripps Research Institute Encoded combinatorial chemical libraries
WO2000023458A1 (en) * 1998-10-19 2000-04-27 The Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library chemistry
WO2002099078A2 (en) * 2001-06-05 2002-12-12 Irm Llc Functional proteomic profiling
WO2004001042A2 (en) * 2002-06-20 2003-12-31 Nuevolution A/S Microarrays displaying encoded molecules
WO2004074501A2 (en) * 2003-02-21 2004-09-02 Nuevolution A/S A method for obtaining structural information about an encoded molecule
WO2006053571A2 (en) * 2004-11-22 2006-05-26 Peter Birk Rasmussen Template directed split and mix systhesis of small molecule libraries

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ANONYMOUS: "Experimental details of library syntheses, DNA microarray and hybridization conditions. PNA code and AA used in the libraries", ELECTRONIC SUPPLEMENTARY INFORMATION FOR CHEMICAL COMMUNICATIONS, pages 1 - 9, XP002506608, Retrieved from the Internet <URL:http://www.rsc.org/suppdata/cc/b4/b415847d/> *
DÍAZ-MOCHÓN JUAN JOSÉ ET AL: "Combinatorial libraries - from solution to 2D microarrays.", CHEMICAL COMMUNICATIONS (CAMBRIDGE, ENGLAND) 21 MAR 2005, no. 11, 21 March 2005 (2005-03-21), pages 1384 - 1386, XP002506607, ISSN: 1359-7345 *

Also Published As

Publication number Publication date
GB0712245D0 (en) 2007-08-01
WO2009001135A2 (en) 2008-12-31

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