WO2009001135A3 - Combinatorial libraries and related methods - Google Patents
Combinatorial libraries and related methods Download PDFInfo
- Publication number
- WO2009001135A3 WO2009001135A3 PCT/GB2008/050495 GB2008050495W WO2009001135A3 WO 2009001135 A3 WO2009001135 A3 WO 2009001135A3 GB 2008050495 W GB2008050495 W GB 2008050495W WO 2009001135 A3 WO2009001135 A3 WO 2009001135A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- molecular constructs
- library
- different
- tag elements
- elements
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Urology & Nephrology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
There is disclosed a method of synthesizing a library of molecular constructs, comprising a plurality of rounds of synthesis, wherein during at least two of the plurality of rounds of synthesis, tag elements from a group of tag elements are combinatorially attached to molecular constructs to form tags, each of which comprises a plurality of said tag elements, to form a library of more diverse molecular constructs such that, after completion of the said plurality of rounds of synthesis, different molecular constructs within the resulting library have different tags attached thereto, the method further comprising the step of separating molecular constructs from a mixture of the resulting molecular constructs using a plurality of specific binding elements each of which specifically binds a different tag element from the group of different tag elements, or a specific combination of different tag elements from the group of different tag elements, to form a combinatorial library of molecular constructs. There is also disclosed a method of screening a library of molecular constructs in which molecular constructs are separated with spatially separate specific binding regions and then remain spatially separated during screening.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0712245.0 | 2007-06-25 | ||
GB0712245A GB0712245D0 (en) | 2007-06-25 | 2007-06-25 | Combinatorial libraries and related methods |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009001135A2 WO2009001135A2 (en) | 2008-12-31 |
WO2009001135A3 true WO2009001135A3 (en) | 2009-03-05 |
Family
ID=38352842
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB2008/050495 WO2009001135A2 (en) | 2007-06-25 | 2008-06-25 | Combinatorial libraries and related methods |
Country Status (2)
Country | Link |
---|---|
GB (1) | GB0712245D0 (en) |
WO (1) | WO2009001135A2 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE602006018648D1 (en) * | 2005-12-01 | 2011-01-13 | Nuevolution As | ENZYMMETRING CODING METHODS FOR EFFICIENT SYNTHESIS OF LARGE LIBRARIES |
CN107849737B (en) * | 2015-07-03 | 2020-06-26 | 湖南中晟全肽生化有限公司 | Peptide library construction method and related vector |
CA3041645C (en) | 2016-10-24 | 2021-11-02 | Geneinfosec, Inc. | Concealing information present within nucleic acids |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5723598A (en) * | 1992-03-30 | 1998-03-03 | The Scripps Research Institute | Encoded combinatorial chemical libraries |
WO2000023458A1 (en) * | 1998-10-19 | 2000-04-27 | The Board Of Trustees Of The Leland Stanford Junior University | Dna-templated combinatorial library chemistry |
WO2002099078A2 (en) * | 2001-06-05 | 2002-12-12 | Irm Llc | Functional proteomic profiling |
WO2004001042A2 (en) * | 2002-06-20 | 2003-12-31 | Nuevolution A/S | Microarrays displaying encoded molecules |
WO2004074501A2 (en) * | 2003-02-21 | 2004-09-02 | Nuevolution A/S | A method for obtaining structural information about an encoded molecule |
WO2006053571A2 (en) * | 2004-11-22 | 2006-05-26 | Peter Birk Rasmussen | Template directed split and mix systhesis of small molecule libraries |
-
2007
- 2007-06-25 GB GB0712245A patent/GB0712245D0/en not_active Ceased
-
2008
- 2008-06-25 WO PCT/GB2008/050495 patent/WO2009001135A2/en active Application Filing
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5723598A (en) * | 1992-03-30 | 1998-03-03 | The Scripps Research Institute | Encoded combinatorial chemical libraries |
US6060596A (en) * | 1992-03-30 | 2000-05-09 | The Scripps Research Institute | Encoded combinatorial chemical libraries |
WO2000023458A1 (en) * | 1998-10-19 | 2000-04-27 | The Board Of Trustees Of The Leland Stanford Junior University | Dna-templated combinatorial library chemistry |
WO2002099078A2 (en) * | 2001-06-05 | 2002-12-12 | Irm Llc | Functional proteomic profiling |
WO2004001042A2 (en) * | 2002-06-20 | 2003-12-31 | Nuevolution A/S | Microarrays displaying encoded molecules |
WO2004074501A2 (en) * | 2003-02-21 | 2004-09-02 | Nuevolution A/S | A method for obtaining structural information about an encoded molecule |
WO2006053571A2 (en) * | 2004-11-22 | 2006-05-26 | Peter Birk Rasmussen | Template directed split and mix systhesis of small molecule libraries |
Non-Patent Citations (2)
Title |
---|
ANONYMOUS: "Experimental details of library syntheses, DNA microarray and hybridization conditions. PNA code and AA used in the libraries", ELECTRONIC SUPPLEMENTARY INFORMATION FOR CHEMICAL COMMUNICATIONS, pages 1 - 9, XP002506608, Retrieved from the Internet <URL:http://www.rsc.org/suppdata/cc/b4/b415847d/> * |
DÍAZ-MOCHÓN JUAN JOSÉ ET AL: "Combinatorial libraries - from solution to 2D microarrays.", CHEMICAL COMMUNICATIONS (CAMBRIDGE, ENGLAND) 21 MAR 2005, no. 11, 21 March 2005 (2005-03-21), pages 1384 - 1386, XP002506607, ISSN: 1359-7345 * |
Also Published As
Publication number | Publication date |
---|---|
GB0712245D0 (en) | 2007-08-01 |
WO2009001135A2 (en) | 2008-12-31 |
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