WO2009000266A1 - Dispositif optique - Google Patents

Dispositif optique Download PDF

Info

Publication number
WO2009000266A1
WO2009000266A1 PCT/DK2008/000230 DK2008000230W WO2009000266A1 WO 2009000266 A1 WO2009000266 A1 WO 2009000266A1 DK 2008000230 W DK2008000230 W DK 2008000230W WO 2009000266 A1 WO2009000266 A1 WO 2009000266A1
Authority
WO
WIPO (PCT)
Prior art keywords
tube
probe
fluid
sensor
opaque
Prior art date
Application number
PCT/DK2008/000230
Other languages
English (en)
Inventor
Holger Dirac
Kasper Paasch
Original Assignee
Diramo A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diramo A/S filed Critical Diramo A/S
Publication of WO2009000266A1 publication Critical patent/WO2009000266A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0325—Cells for testing reactions, e.g. containing reagents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0346—Capillary cells; Microcells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"

Definitions

  • the invention relates to a device for analyzing a medium where the analyzing is based on optical detection.
  • the invention relates to a device comprising an optical sensor, a probe for collecting a sample of the medium, and a tube forming a conduit between the probe and the optical sensor.
  • Analyzing tools which are based on optical detection, e.g. spectroscopy tools, are based on a study of the optical properties of the medium, e.g. the intensity of emitted light. Such tools and processes are used, e.g. for measuring the concentration of species in the medium in chemistry.
  • signal intensity is the intensity of the electromagnetic radiation which is measured.
  • tools of the kind to which the invention relates comprises sensing means capable of optically detecting characteristics of a medium, a probe by which a sample of the medium is collected, and a tube for transporting sample between probe and the sensing means.
  • the probe comprises a membrane which is open to the permeation of species of interest, which e.g. can be arranged subcutaneously or intravenously, and along which a perfusion fluid is circulated.
  • the perfusion fluid sample transports substances from the membrane to an analysing means which optically detects characteristics (e.g. absorption and emission characteristics) of the sample, and by processing of the data concentrations of various elements in the sample can be determined - possibly by using a blank sample as a reference.
  • the tube connecting the probe placed (e.g. in-situ/in-vivo) in the medium and the analysing means can unfortunately transmit and even collect ambient light.
  • the tube material and/or the sample inside the tube may act as an optical waveguide to transfer unwanted light to the analysing means and the optical detector. This will influence the measurement and limit detection range and accuracy.
  • the object of this invention is to prevent unwanted light from entering the analysing means and optical detector in devices that are intended to measure e.g. in-situ/in-vivo and thus are connected to the medium under analysis.
  • the invention in a first aspect provides a device wherein the tube is at least partly comprised by an opaque or absorbing material.
  • the tube wall thus is opaque or absorbing, electromagnetic radiation, e.g. light from an ambient space, is at least partly prevented from entering into the conduit where it could possibly reach the analysing means, and the risk of erroneous measurements is reduced.
  • electromagnetic radiation e.g. light from an ambient space
  • opaque or absorbing wall should be understood as a wall which essentially prevents electromagnetic radiation at any desired wave length, e.g. in the wave length of 200-1100 nm, or even more particularly visible light, from passing through the wall.
  • a pigment could be added to a base material from which the tube is made.
  • the tube could be made from a relatively dark- coloured material, e.g. from a black material, or dark blue or dark gray material.
  • coloured means that the tube has a colour which prevents or reduces propagation of light through the tube and which may further prevent or reduce entrance of light across the wall of the tube.
  • the device according to the present invention may e.g. be adapted for electromagnetic radiation within the above mentioned range of 200-1100 nm.
  • the device may, however, be adapted for any range of wavelength.
  • optical sensor is hereby defined a sensor which is sensitive to electromagnetic radiation, in particular within 200-1100 nm and which forms part of the analysing means.
  • the optical sensor is typically housed in a housing which in addition may comprise a power supply, a pump, a reservoir for a sampling fluid, various filters and a processing unit for analyzing the signal from the sensor.
  • the tube could be made from any material, typically a polymer material.
  • the tube could have at least one inner conduit or lumen for conducting the sample in question.
  • the conduit may have a size in the range of 0.1 - 0.3 mm, such as 0.2 mm. and the tube may have an outer diameter in the range of 0.4-0.6 mm, such as 0.5 mm, i.e. the tube may have a wall thickness e.g. in the size of 0.15 mm.
  • the tube may form two conduits each having a cross-section of 0.17-0.19 mm, such as 0.18 mm.
  • One of the conduits feeding a fluid to a membrane in the probe and one of the conduits conducting the fluid from the membrane back to the sensor.
  • the tube can be placed inside second tube, which substantially prevents light from entering the first tube.
  • the plastic material may be coloured with a pigment during the manufacturing of the tube so that the tube is coloured throughout its wall.
  • the tube is provided with an inner surface or an outer surface being coated with a non-transparent material, e.g. with a metallic coating, or it could be painted with a dark and preferably non-reflective colour, e.g. black.
  • a non-transparent material e.g. with a metallic coating
  • the tube may comprise an intermediate layer between the inner and outer surface of the tube, the intermediate layer being a metallic layer or a layer which reflects or absorbs electromagnetic radiation, or the tube may simply contain a sufficient amount of metallic components or other components which reflect or absorb electromagnetic radiation.
  • the tube may be bio-compatible or coated with a bio-compatible coating.
  • the wall may in particular have a very high optical absorbance.
  • an inner surface of the tube may have a non-reflective surface.
  • a is an absorption length measured in 1 /length, e.g. 1/mm.
  • the absorption length is defined by the wall thickness of the tube.
  • the absorption length is preferably significantly below 1 mm "1 .
  • the devices for which this invention could be applicable could be e.g. a spectrophotometer, a bio or chemo luminance device, a fluorescent spectrometer, etc.
  • the opaque or absorbing wall may constitute a portion of the tube, or it may constitute the entire tube. In particular, it may constitute a portion very near to the optical sensor.
  • the probe comprises a membrane, and the sampling takes place by conducting a sampling fluid from a reservoir via an additional tube to the membrane.
  • the sampling fluid absorbs a substance to be analyzed, and the substance is transported with the sampling fluid back to the optical sensor via the tube.
  • Both tubes may be opaque or absorbing, and both tubes may be formed as a single tube having two or more conduits - sometimes referred to as a multilumen tube.
  • the device may comprise at least one tube for conducting at least one reagent which is mixed with the fluid sample.
  • the tubes for conducting the reagents may have an opaque or absorbing wall. Any other tubes in connection with the analysing means may also have an opaque or absorbing wall.
  • the probe may contain the membrane in a housing which in general has properties towards electromagnetic radiation being similar to those of the tube, i.e. reflective and/or absorbing.
  • the invention provides a method of collecting a fluid sample, the method comprising: providing a sensor and a probe, the sensor and probe being in fluid communication via a tube, providing a tube being opaque or absorbing, inserting the tube into a fluid medium and transporting a sample of the fluid medium through the tube to the sensor.
  • the invention provides a tube forming a fluid conduit and comprises coupling means for attaching the tube between a probe and a sensor, characterized in that the tube comprises an opaque or absorbing wall.
  • Fig. 1 illustrates a device according to the invention
  • Fig. 2 illustrates schematically an embodiment of an analysis device
  • Fig. 3 illustrates entrance of light into the tube
  • Fig. 4 illustrates an arm of a human being with a probe for a device according to the invention.
  • the device comprises a probe 1 with a membrane 2 which is immersed in a medium 3 which is to be analyzed, such as a fluid like liquid, gas or animal tissue or body fluids.
  • a first tube 4 conducts a perfusion fluid from a reservoir, typically arranged in a fixed workstation 5 to an inner portion of the membrane 2.
  • the perfusion fluid collects substances of interest, and by use of a pressure difference, the fluid is conducted in the second tube 6 to an analysis system 7.
  • the perfusion fluid is simply a saline solution.
  • the analysis system comprises an optical sensor 8, e.g. in the form of a single chip sensor.
  • the fixed workstation 5 typically contains a pump, a reservoir containing reagents, a power supply, a monitor, a computer etc.
  • some of the mentioned facilities can be comprised in the analysis system 7.
  • Fig. 2 illustrates schematically an embodiment of an analysis system 7 comprising a microfluid canal 11 and an optical sensor 8.
  • the substance enriched sample fluid is conducted to the canal 11 via the second tube 6, where different reagents are added.
  • the reagents are provided via additional tubes 9, 10.
  • the number of additional tubes depends on the number of separate reagents which are necessary.
  • the tubes 9, 10 are connected to the reagent reservoir in the fixed workstation 5.
  • the reactions obtained thereby will provide florescence or luminescence and a light will 15 thereby be emitted with an intensity which depends on the concentration of the substances in question.
  • the emitted light is visualized by the lines 12.
  • the light (the photons) will be detected by the optical sensor 8 which typically will be in communication with a computer structure contained in the workstation 5 or contained in the analysis system 7.
  • Fig. 3 illustrates that light 12 may enter the second tube 6 and thereby enter the sample fluid in the canal 11. Such light will be measurable by the optical sensor 8 which thereby provides a false measurement.
  • the light could enter the tube, e.g. at the end face 13 and at the opening 14 into the tube, but are, given the small dimensions of the tube, most likely to enter through the side of the tube.
  • the light may propagate to the location of the optical sensor and thereby provide false measurements. If, however, the tube is coated, or generally made from a material which reflects or absorbs the light, propagation of light across the wall can be limited or prevented, and the measurements may become more exact.
  • the arrows illustrate how the perfusion fluid circulates at the membrane from the first tube 4 towards the second tube 6 and during the passage through the membrane collects the substances through which they diffuse.
  • the membrane is illustrated by the dotted line.
  • the second tube 6 is black, or at leas very dark coloured in order thereby to ensure that light which may enter the tube is dampened or eliminated completely.
  • Fig. 4 illustrates the probe 2 inserted via a catheter 15 into a body of a human being, where the media under investigation is human tissue or bodily fluids such as blood, where it is not possible to a calibration to some standard situation, since the person or patient under investigation may be active and moving around. Therefore it would be critical to eliminate any incoming light to the tubes and thereby to the analysing system and optical sensor.

Abstract

L'invention concerne un dispositif comprenant un capteur pour détecter un rayonnement électromagnétique tel que la lumière. Ce capteur communique avec une sonde par l'intermédiaire d'un tube. Afin de prévenir les erreurs de mesure, le tube est formé avec une paroi opaque ou colorée qui empêche le passage du rayonnement électromagnétique à travers sa paroi, et réduit la propagation de la lumière à travers son conduit interne.
PCT/DK2008/000230 2007-06-22 2008-06-19 Dispositif optique WO2009000266A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200700909 2007-06-22
DKPA200700909 2007-06-22

Publications (1)

Publication Number Publication Date
WO2009000266A1 true WO2009000266A1 (fr) 2008-12-31

Family

ID=39924958

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2008/000230 WO2009000266A1 (fr) 2007-06-22 2008-06-19 Dispositif optique

Country Status (1)

Country Link
WO (1) WO2009000266A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3200254A (en) * 1962-03-19 1965-08-10 Gen Electric Photosensitive detector for airborne particles
US6011882A (en) * 1997-10-16 2000-01-04 World Precision Instruments, Inc. Chemical sensing techniques employing liquid-core optical fibers
WO2001053807A1 (fr) * 2000-01-22 2001-07-26 Global Fia, Inc. Detecteur de luminescence pourvu d'un guide d'ondes a coeur liquide
US20020102183A1 (en) * 2001-01-29 2002-08-01 Horiba, Ltd. Cell for analyzing fluid and analyzing apparatus using the same
US6558626B1 (en) * 2000-10-17 2003-05-06 Nomadics, Inc. Vapor sensing instrument for ultra trace chemical detection
US20050078308A1 (en) * 2002-02-25 2005-04-14 Waters Investments Limited Opaque additive to block stray light in teflon® af light-guiding flowcells

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3200254A (en) * 1962-03-19 1965-08-10 Gen Electric Photosensitive detector for airborne particles
US6011882A (en) * 1997-10-16 2000-01-04 World Precision Instruments, Inc. Chemical sensing techniques employing liquid-core optical fibers
WO2001053807A1 (fr) * 2000-01-22 2001-07-26 Global Fia, Inc. Detecteur de luminescence pourvu d'un guide d'ondes a coeur liquide
US6558626B1 (en) * 2000-10-17 2003-05-06 Nomadics, Inc. Vapor sensing instrument for ultra trace chemical detection
US20020102183A1 (en) * 2001-01-29 2002-08-01 Horiba, Ltd. Cell for analyzing fluid and analyzing apparatus using the same
US20050078308A1 (en) * 2002-02-25 2005-04-14 Waters Investments Limited Opaque additive to block stray light in teflon® af light-guiding flowcells

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