WO2008157827A3 - Modified composition of plant extracellular lipids using acyltransferases and fatty acid omega-oxidases - Google Patents

Modified composition of plant extracellular lipids using acyltransferases and fatty acid omega-oxidases Download PDF

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Publication number
WO2008157827A3
WO2008157827A3 PCT/US2008/067887 US2008067887W WO2008157827A3 WO 2008157827 A3 WO2008157827 A3 WO 2008157827A3 US 2008067887 W US2008067887 W US 2008067887W WO 2008157827 A3 WO2008157827 A3 WO 2008157827A3
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WO
WIPO (PCT)
Prior art keywords
fatty acid
acyltransferases
oxidases
modified composition
extracellular lipids
Prior art date
Application number
PCT/US2008/067887
Other languages
French (fr)
Other versions
WO2008157827A2 (en
Inventor
John B Ohlrogge
Fred Beisson
Yonghua Li
Mike Pollard
Original Assignee
Univ Michigan State
John B Ohlrogge
Fred Beisson
Yonghua Li
Mike Pollard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Michigan State, John B Ohlrogge, Fred Beisson, Yonghua Li, Mike Pollard filed Critical Univ Michigan State
Publication of WO2008157827A2 publication Critical patent/WO2008157827A2/en
Publication of WO2008157827A3 publication Critical patent/WO2008157827A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Constructs and methods are provided for altering extracellular lipids in plants. In general, the methods include increasing expression of an acyltransferase and a fatty acid oxidase in the plant.
PCT/US2008/067887 2007-06-21 2008-06-23 Modified composition of plant extracellular lipids using acyltransferases and fatty acid omega-oxidases WO2008157827A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US93658307P 2007-06-21 2007-06-21
US60/936,583 2007-06-21

Publications (2)

Publication Number Publication Date
WO2008157827A2 WO2008157827A2 (en) 2008-12-24
WO2008157827A3 true WO2008157827A3 (en) 2009-06-04

Family

ID=40156981

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/067887 WO2008157827A2 (en) 2007-06-21 2008-06-23 Modified composition of plant extracellular lipids using acyltransferases and fatty acid omega-oxidases

Country Status (1)

Country Link
WO (1) WO2008157827A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9351507B2 (en) 2006-07-14 2016-05-31 Commonwealth Scientific And Industrial Research Organisation Method of preparing food using rice oil

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2009273754B2 (en) 2008-07-21 2016-07-14 Commonwealth Scientific And Industrial Research Organisation Improved cottonseed oil and uses
WO2010009500A1 (en) 2008-07-21 2010-01-28 Commonwealth Scientific And Industrial Research Organisation Improved vegetable oils and uses therefor
AR083323A1 (en) 2010-06-28 2013-02-21 Commw Scient Ind Res Org METHODS TO PRODUCE LIPIDS
MX2014007964A (en) 2011-12-27 2014-10-06 Commw Scient Ind Res Org Processes for producing lipids.
US8809026B2 (en) 2011-12-27 2014-08-19 Commonwealth Scientific And Industrial Research Organisation Processes for producing lipids
US11639507B2 (en) 2011-12-27 2023-05-02 Commonwealth Scientific And Industrial Research Organisation Processes for producing lipids
US9096834B2 (en) 2012-02-24 2015-08-04 Exxonmobil Research And Engineering Company Recombinant microorganisms comprising thioesterase and lysophosphatidic acid acyltransferase genes for fatty acid production
WO2013159149A1 (en) 2012-04-25 2013-10-31 Commonwealth Scientific And Industrial Research Organisation High oleic acid oils
EP2966157B1 (en) 2014-07-07 2023-11-01 Nuseed Global Innovation Ltd Processes for producing industrial products from plant lipids
BR112019004530A2 (en) 2016-09-02 2019-10-22 Commw Scient Ind Res Org plants with modified traits

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
BEISSON FRED ET AL: "The acyltransferase GPAT5 is required for the synthesis of suberin in seed coat and root of Arabidopsis", PLANT CELL, vol. 19, no. 1, January 2007 (2007-01-01), pages 351 - 368, XP002523671, ISSN: 1040-4651 *
BENVENISTE ET AL: "CYP86A1 from Arabidopsis thaliana encodes a cytochrome P450-dependent fatty acid omega-hydroxylase", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 243, 1 February 1998 (1998-02-01), pages 688 - 693, XP002091710, ISSN: 0006-291X *
LI YONGHUA ET AL: "Identification of acyltransferases required for cutin biosynthesis and production of cutin with suberin-like monomers", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 104, no. 46, November 2007 (2007-11-01), pages 18339 - 18344, XP002523673, ISSN: 0027-8424 *
LI YONGHUA ET AL: "Monoacylglycerols are components of root waxes and can be produced in the aerial cuticle by ectopic expression of a suberin-associated acyltransferase", PLANT PHYSIOLOGY (ROCKVILLE), vol. 144, no. 3, 11 May 2007 (2007-05-11), pages 1267 - 1277, XP002523668, ISSN: 0032-0889 *
POLLARD M ET AL: "Building lipid barriers: biosynthesis of cutin and suberin", TRENDS IN PLANT SCIENCE, ELSEVIER SCIENCE, OXFORD, GB, vol. 13, no. 5, 1 May 2008 (2008-05-01), pages 236 - 246, XP022649929, ISSN: 1360-1385, [retrieved on 20080424] *
SOLER MARCAL ET AL: "A genomic approach to suberin biosynthesis and cork differentiation", PLANT PHYSIOLOGY (ROCKVILLE), vol. 144, no. 1, May 2007 (2007-05-01), pages 419 - 431, XP002523669, ISSN: 0032-0889 *
SUH MI CHUNG ET AL: "Cuticular lipid composition, surface structure, and gene expression in Arabidopsis stem epidermis", PLANT PHYSIOLOGY (ROCKVILLE), vol. 139, no. 4, December 2005 (2005-12-01), pages 1649 - 1665, XP002523667, ISSN: 0032-0889 *
WELLESEN KIRSTEN ET AL: "Functional analysis of the LACERATA gene of Arabidopsis provides evidence for different roles of fatty acid omega-hydroxylation in development", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 98, no. 17, 14 August 2001 (2001-08-14), pages 9694 - 9699, XP002523670, ISSN: 0027-8424 *
XIAO FANGMING ET AL: "Arabidopsis CYP86A2 represses Pseudomonas syringae type III genes and is required for cuticle development", EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 23, no. 14, 21 July 2004 (2004-07-21), pages 2903 - 2913, XP002523672, ISSN: 0261-4189 *
YOKOI S ET AL: "INTRODUCTION OF THE CDNA FOR ARABIDOPSIS GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE (GPAT) CONFERS UNSATURATION OF FATTY ACIDS AND CHILLING TOLERANCE OF PHOTOSYNTHESIS ON RICE", MOLECULAR BREEDING: NEW STRATEGIES IN PLANT IMPROVEMENT, KLUWER ACADEMIC PUBLISHERS, NL, vol. 4, no. 3, 1 January 1998 (1998-01-01), pages 269 - 275, XP000909905, ISSN: 1380-3743 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9351507B2 (en) 2006-07-14 2016-05-31 Commonwealth Scientific And Industrial Research Organisation Method of preparing food using rice oil

Also Published As

Publication number Publication date
WO2008157827A2 (en) 2008-12-24

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