WO2008156048A1 - 酵母細胞セット、標的候補分子同定方法、作用機序解析方法及びスクリーニング方法 - Google Patents

酵母細胞セット、標的候補分子同定方法、作用機序解析方法及びスクリーニング方法 Download PDF

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Publication number
WO2008156048A1
WO2008156048A1 PCT/JP2008/060963 JP2008060963W WO2008156048A1 WO 2008156048 A1 WO2008156048 A1 WO 2008156048A1 JP 2008060963 W JP2008060963 W JP 2008060963W WO 2008156048 A1 WO2008156048 A1 WO 2008156048A1
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WIPO (PCT)
Prior art keywords
yeast cells
target candidate
action mechanism
candidate molecule
identifying target
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PCT/JP2008/060963
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English (en)
French (fr)
Inventor
Kappei Tsukahara
Kaoru Mitsuhashi
Makiyo Iida
Original Assignee
Eisai R & D Management Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eisai R & D Management Co., Ltd. filed Critical Eisai R & D Management Co., Ltd.
Priority to CN200880020854A priority Critical patent/CN101688199A/zh
Priority to JP2009520470A priority patent/JPWO2008156048A1/ja
Priority to US12/665,522 priority patent/US20100240074A1/en
Priority to AU2008264625A priority patent/AU2008264625A1/en
Priority to CA002691651A priority patent/CA2691651A1/en
Priority to EP08765646A priority patent/EP2169056A4/en
Publication of WO2008156048A1 publication Critical patent/WO2008156048A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

　薬物感受性が著しく向上した酵母遺伝子破壊株セットを提供する。 　本発明の酵母細胞セットは、２種以上の酵母細胞からなる酵母細胞セットであって、前記２種以上の酵母細胞の各々が相互に異なる遺伝子の少なくとも１種を欠損又は変異し、かつ、前記２種以上の酵母細胞のすべてが薬物感受性関連遺伝子の少なくとも１種を発現制御された薬物高感受性酵母細胞からなる酵母細胞セットである。
PCT/JP2008/060963 2007-06-18 2008-06-16 酵母細胞セット、標的候補分子同定方法、作用機序解析方法及びスクリーニング方法 WO2008156048A1 (ja)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CN200880020854A CN101688199A (zh) 2007-06-18 2008-06-16 酵母细胞集、目标候选分子鉴定方法、作用机理解析方法和筛选方法
JP2009520470A JPWO2008156048A1 (ja) 2007-06-18 2008-06-16 酵母細胞セット、標的候補分子同定方法、作用機序解析方法及びスクリーニング方法
US12/665,522 US20100240074A1 (en) 2007-06-18 2008-06-16 Set of Yeast Cells, Method of Identifying Target Candidate Molecule, Method of Analyzing Action Mechanism and Screening Method
AU2008264625A AU2008264625A1 (en) 2007-06-18 2008-06-16 Set of yeast cells, method of identifying target candidate molecule, method of analyzing action mechanism and screening method
CA002691651A CA2691651A1 (en) 2007-06-18 2008-06-16 Set of yeast cells, method of identifying target candidate molecule, method of analyzing action mechanism and screening method
EP08765646A EP2169056A4 (en) 2007-06-18 2008-06-16 YELLOW CELL ASSEMBLY, METHOD FOR IDENTIFYING TARGET CANDIDATE MOLECULE, METHOD FOR ANALYZING ACTION MECHANISM, AND SCREENING METHOD

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007160457 2007-06-18
JP2007-160457 2007-06-18

Publications (1)

Publication Number Publication Date
WO2008156048A1 true WO2008156048A1 (ja) 2008-12-24

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Application Number Title Priority Date Filing Date
PCT/JP2008/060963 WO2008156048A1 (ja) 2007-06-18 2008-06-16 酵母細胞セット、標的候補分子同定方法、作用機序解析方法及びスクリーニング方法

Country Status (8)

Country Link
US (1) US20100240074A1 (ja)
EP (1) EP2169056A4 (ja)
JP (1) JPWO2008156048A1 (ja)
KR (1) KR20100044175A (ja)
CN (1) CN101688199A (ja)
AU (1) AU2008264625A1 (ja)
CA (1) CA2691651A1 (ja)
WO (1) WO2008156048A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016505270A (ja) * 2013-01-24 2016-02-25 ダンスター・フェルメント・アーゲー ビタミンｄ２を含む酵母細胞壁、その使用、およびその産生方法
JP2019068843A (ja) * 2018-12-28 2019-05-09 ダンスター・フェルメント・アーゲー ビタミンｄ２を含む酵母細胞壁、その使用、およびその産生方法
JP2021010306A (ja) * 2019-07-04 2021-02-04 国立大学法人 東京大学 抗真菌剤のスクリーニング方法、及び、真菌病原性発現阻害剤である抗真菌剤

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112646811B (zh) * 2020-12-21 2022-09-06 广东省微生物研究所（广东省微生物分析检测中心） 一种适用于酵母多基因敲除的CRISPR/Cas9系统及其构建方法和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006046694A1 (ja) 2004-10-29 2006-05-04 Eisai R & D Management Co., Ltd. 誘導可能に発現するエルゴステロール合成酵素を有する酵母を利用した遺伝子のスクリーニング法
JP2006141298A (ja) 2004-11-19 2006-06-08 Eisai Co Ltd 細胞に対する被検物質の評価

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006046694A1 (ja) 2004-10-29 2006-05-04 Eisai R & D Management Co., Ltd. 誘導可能に発現するエルゴステロール合成酵素を有する酵母を利用した遺伝子のスクリーニング法
JP2006141298A (ja) 2004-11-19 2006-06-08 Eisai Co Ltd 細胞に対する被検物質の評価

Non-Patent Citations (25)

* Cited by examiner, † Cited by third party
Title
"Methods in Yeast Genetics: A Cold Spring Harber Laboratory Course Manual", 2000
ANDERSON J.E. ET AL.: "Antagonism between two mechanisms of antifungal drug resistance", EUKARYOT CELL, vol. 5, no. 8, August 2006 (2006-08-01), pages 1243 - 1251, XP008134909 *
BAUDIN A ET AL., NUCL.ACID RES., vol. 21, 1993, pages 3329 - 3330
BAUDIN A, NUCL.ACID RES., vol. 21, 1993, pages 3329 - 3330
DE- LAVEAU ET AL., MOL GEN GENET., vol. 244, no. 5, 1994, pages 501 - 11
DELAVEAU T.: "PDDR3, a new yeast regulatory gene, is homologue to PDR1 and controls the multidrug resistance phenomenon", MOL. GEN. GENET., vol. 244, no. 5, 1 September 1994 (1994-09-01), pages 501 - 511, XP008134910 *
GIAEVER ET AL., NAT GENET, vol. 21, 1999, pages 278 - 83
GIAEVER ET AL., NATURE GENETICS, vol. 21, 1999, pages 278 - 283
GIAEVER ET AL., NATURE, vol. 418, 2002, pages 387 - 91
GIAEVER ET AL., PROC NATL ACAD SCI USA, vol. 101, 2004, pages 793 - 798
GIAEVER G. ET AL.: "Chemogenomic profiling: identifying the functional interactions of small molecules in yeast", PROC. NATL. ACAD. SCI. USA, vol. 101, no. 3, 20 January 2004 (2004-01-20), pages 793 - 798, XP008127226 *
GIAEVER G. ET AL.: "Genomic profiling of drug sensitivites via induced haploinsufficiency", NAT. GENET., vol. 21, no. 3, March 1999 (1999-03-01), pages 278 - 283, XP001025981 *
HARTWELL ET AL., SCIENCE, vol. 278, 1997, pages 1064 - 8
HEITMAN ET AL., PROC NATL ACAD SCI U S A., vol. 88, 1991, pages 1948 - 52
HEITMAN J ET AL.: "FK 506-binding protein proline rotamase is a target for the immunosuppressive agent FK 506 in Saccharomyces cerevisiae", PROC NATL ACAD SCI USA, vol. 88, no. 5, 1991, pages 1948 - 52
HUELS ET AL., DRUG DISCOVERY TODAY, vol. 7, 2002, pages 119 - 124
LUM ET AL., CELL, vol. 116, 2004, pages 121 - 37
LUM P.Y. ET AL.: "Discovering modes of action for therapeutic compounds using a genome-wide screen of yeast heterozygotes", CELL, vol. 116, no. 1, 9 January 2004 (2004-01-09), pages 121 - 137, XP008077831 *
PARSONS A.B. ET AL.: "Integration of chemical-genetic and genetic interaction data links bioactive compounds to cellular target pathways", NAT. BIOTECHNOL., vol. 22, no. 1, January 2004 (2004-01-01), pages 62 - 69, XP008127228 *
PARSONS ET AL., NAT BIOTECHNOL, vol. 22, 2004, pages 62 - 9
SCIENCE, vol. 294, 2001, pages 2364 - 2368
SCIENCE, vol. 294, no. 5550, 14 December 2001 (2001-12-14), pages 2364 - 2368
See also references of EP2169056A4
SIMON; BEDALOV, NAT REV CANCER, vol. 4, 2004, pages 481 - 487
STEPANOV A. ET AL.: "Enhancing drug accumulation in S. cereviase by repression of pleiotropic drug resistance genes with chimeric transcription repressors", MOL. PHARMACOL., 9 May 2008 (2008-05-09), XP008127256 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016505270A (ja) * 2013-01-24 2016-02-25 ダンスター・フェルメント・アーゲー ビタミンｄ２を含む酵母細胞壁、その使用、およびその産生方法
JP2019068843A (ja) * 2018-12-28 2019-05-09 ダンスター・フェルメント・アーゲー ビタミンｄ２を含む酵母細胞壁、その使用、およびその産生方法
JP2021010306A (ja) * 2019-07-04 2021-02-04 国立大学法人 東京大学 抗真菌剤のスクリーニング方法、及び、真菌病原性発現阻害剤である抗真菌剤
JP7345122B2 (ja) 2019-07-04 2023-09-15 国立大学法人 東京大学 抗真菌剤のスクリーニング方法、及び、真菌病原性発現阻害剤である抗真菌剤

Also Published As

Publication number Publication date
KR20100044175A (ko) 2010-04-29
US20100240074A1 (en) 2010-09-23
CN101688199A (zh) 2010-03-31
AU2008264625A1 (en) 2008-12-24
CA2691651A1 (en) 2008-12-24
EP2169056A1 (en) 2010-03-31
EP2169056A4 (en) 2011-07-06
JPWO2008156048A1 (ja) 2010-08-26

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