WO2008151386A8 - Megakaryocyte differentiation - Google Patents

Megakaryocyte differentiation Download PDF

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Publication number
WO2008151386A8
WO2008151386A8 PCT/AU2008/000861 AU2008000861W WO2008151386A8 WO 2008151386 A8 WO2008151386 A8 WO 2008151386A8 AU 2008000861 W AU2008000861 W AU 2008000861W WO 2008151386 A8 WO2008151386 A8 WO 2008151386A8
Authority
WO
WIPO (PCT)
Prior art keywords
scf
tpo
formation
under conditions
conditions sufficient
Prior art date
Application number
PCT/AU2008/000861
Other languages
French (fr)
Other versions
WO2008151386A1 (en
Inventor
Andrew Elefanty
Eduoard Stanley
Elizabeth Ng
Original Assignee
Australian Stem Cell Ct Ltd
Andrew Elefanty
Eduoard Stanley
Elizabeth Ng
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2007903224A external-priority patent/AU2007903224A0/en
Application filed by Australian Stem Cell Ct Ltd, Andrew Elefanty, Eduoard Stanley, Elizabeth Ng filed Critical Australian Stem Cell Ct Ltd
Publication of WO2008151386A1 publication Critical patent/WO2008151386A1/en
Publication of WO2008151386A8 publication Critical patent/WO2008151386A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0644—Platelets; Megakaryocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/145—Thrombopoietin [TPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/165—Vascular endothelial growth factor [VEGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides a method for generating megakaryocytes and/or megakaryocyte precursors from a population of embryonic stem cells (ESCs), the method comprising: (i) culturing ESCs in a serum-free, stromal/feeder cell-free medium for a time and under conditions sufficient for formation of mesoderm and/or mesendoderm; and (ii) differentiating the cells cultured in step (i) in a medium comprising thrombopoietin (TPO), stem cell factor (SCF) and interleukin 3 (IL-3), or any functional fragment, variant or mimetic of TPO, SCF and/or IL-3, for a time and under conditions sufficient for formation of megakaryocytes and/or megakaryocyte precursors.
PCT/AU2008/000861 2007-06-15 2008-06-13 Megakaryocyte differentiation WO2008151386A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2007903224 2007-06-15
AU2007903224A AU2007903224A0 (en) 2007-06-15 Megakaryocyte differentiation

Publications (2)

Publication Number Publication Date
WO2008151386A1 WO2008151386A1 (en) 2008-12-18
WO2008151386A8 true WO2008151386A8 (en) 2009-02-05

Family

ID=40129148

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU2008/000861 WO2008151386A1 (en) 2007-06-15 2008-06-13 Megakaryocyte differentiation

Country Status (1)

Country Link
WO (1) WO2008151386A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9938500B2 (en) 2006-04-14 2018-04-10 Astellas Institute For Regenerative Medicine Hemangio-colony forming cells

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2010012088A (en) 2008-05-06 2011-07-28 Advanced Cell Tech Inc Hemangio colony forming cells and non-engrafting hemangio cells.
US20110086424A1 (en) 2008-05-06 2011-04-14 Advanced Cell Technology, Inc. Methods for producing enucleated erythroid cells derived from pluripotent stem cells
CN101864396B (en) * 2010-05-17 2012-08-22 中国人民解放军军事医学科学院野战输血研究所 Method for inducing megakaryoblast and megakaryocyte in vitro
WO2012061146A1 (en) * 2010-10-25 2012-05-10 The Children's Hospital Of Philadelphia Compositions and methods for the generation of platelets and methods of use thereof
GB201210857D0 (en) 2012-06-19 2012-08-01 Cambridge Entpr Ltd Transcription factor mediated programming towards megakaryocytes
CA2896053A1 (en) 2012-12-21 2014-06-26 Ocata Therapeutics, Inc. Methods for production of platelets from pluripotent stem cells and compositions thereof
ES2536605B1 (en) * 2013-10-24 2016-03-02 Fundación Pública Andaluza Progreso Y Salud Method of obtaining megakaryocytes and platelets
CN105749252A (en) * 2016-04-29 2016-07-13 南方医科大学 Application of IL-9 serving as medicine for treating thrombocytopenia
CN109562128A (en) * 2016-08-04 2019-04-02 西奈山伊坎医学院 Produce megacaryocyte composition and the therapy for treating decrease of platelet
CN113884682B (en) * 2021-04-30 2023-07-21 中国医学科学院血液病医院(中国医学科学院血液学研究所) Use of products for detecting megakaryocyte or platelet surface marker molecules in the preparation of products for detecting infections

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2004291559B2 (en) * 2003-11-19 2008-10-16 Australian Stem Cell Centre Limited Methods for producing blood products from pluripotent cells in cell culture
EP1807507A2 (en) * 2004-11-01 2007-07-18 Wisconsin Alumni Research Foundation Platelets from stem cells

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9938500B2 (en) 2006-04-14 2018-04-10 Astellas Institute For Regenerative Medicine Hemangio-colony forming cells

Also Published As

Publication number Publication date
WO2008151386A1 (en) 2008-12-18

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