WO2008137728A8 - System and method for proteomics - Google Patents

System and method for proteomics Download PDF

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Publication number
WO2008137728A8
WO2008137728A8 PCT/US2008/062482 US2008062482W WO2008137728A8 WO 2008137728 A8 WO2008137728 A8 WO 2008137728A8 US 2008062482 W US2008062482 W US 2008062482W WO 2008137728 A8 WO2008137728 A8 WO 2008137728A8
Authority
WO
WIPO (PCT)
Prior art keywords
immobilized
buffers
protein
layer
gel
Prior art date
Application number
PCT/US2008/062482
Other languages
French (fr)
Other versions
WO2008137728A1 (en
Inventor
Jack Johansen
Russell K. Garlick
William Skea
Stephen Haralampu
Oren Kagan
Original Assignee
Protein Forest, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Protein Forest, Inc. filed Critical Protein Forest, Inc.
Priority to JP2010506685A priority Critical patent/JP2010526301A/en
Priority to AU2008247532A priority patent/AU2008247532A1/en
Priority to CA002686254A priority patent/CA2686254A1/en
Priority to EP08747539A priority patent/EP2153218A1/en
Publication of WO2008137728A1 publication Critical patent/WO2008137728A1/en
Publication of WO2008137728A8 publication Critical patent/WO2008137728A8/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Significantly higher yield and better resolution in pi gels are obtained by creating traps having two or more layers of gel (26, 28) containing closely stepped immobilized pH buffers. Proteins move from a pH at which they are negatively charged towards an anode at which they are positively charged. The gel layers (26, 28) containing immobilized pH buffers trap the proteins when the immobilized buffer pH and the protein pi are approximately the same. The protein is trapped within the second layer and not on the surface of or interface of the second layer.
PCT/US2008/062482 2007-05-03 2008-05-02 System and method for proteomics WO2008137728A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2010506685A JP2010526301A (en) 2007-05-03 2008-05-02 Proteomics systems and methods
AU2008247532A AU2008247532A1 (en) 2007-05-03 2008-05-02 System and method for proteomics
CA002686254A CA2686254A1 (en) 2007-05-03 2008-05-02 System and method for proteomics
EP08747539A EP2153218A1 (en) 2007-05-03 2008-05-02 System and method for proteomics

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/744,060 US20080272002A1 (en) 2007-05-03 2007-05-03 System and Method for Proteomics
US11/744,060 2007-05-03

Publications (2)

Publication Number Publication Date
WO2008137728A1 WO2008137728A1 (en) 2008-11-13
WO2008137728A8 true WO2008137728A8 (en) 2009-12-23

Family

ID=39560867

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/062482 WO2008137728A1 (en) 2007-05-03 2008-05-02 System and method for proteomics

Country Status (6)

Country Link
US (1) US20080272002A1 (en)
EP (1) EP2153218A1 (en)
JP (1) JP2010526301A (en)
AU (1) AU2008247532A1 (en)
CA (1) CA2686254A1 (en)
WO (1) WO2008137728A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2853891B1 (en) 2009-08-24 2017-01-04 Life Technologies Corporation Electrophoresis gel cassette and comb
USD719277S1 (en) 2010-08-24 2014-12-09 Life Technologies Corporation Electrophoresis wedge-well comb
USD794823S1 (en) 2010-08-24 2017-08-15 Life Technologies Corporation Electrophoresis tank with a base and cassette inserted in
USD806894S1 (en) 2015-03-19 2018-01-02 Life Technologies Corporation Electrophoresis comb with wedge-shaped teeth
CN107831313A (en) * 2017-10-16 2018-03-23 广州和实生物技术有限公司 The preparation and application of vertical aobvious protein electrophoresis pre-prepared colloid tablet

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE314227B (en) * 1964-08-26 1969-09-01 Lkb Produkter Ab
US5447612A (en) * 1993-02-01 1995-09-05 Protein Technologies, Inc. Buffering system and its use in electrophoretic processes
US5464517A (en) * 1995-01-30 1995-11-07 Bio-Rad Laboratories Electrophoresis in low conductivity buffers
WO1997017384A1 (en) * 1995-11-08 1997-05-15 Kirkpatrick Francis H Methods and reagents for gel electrophoresis
SE9803224D0 (en) * 1998-09-23 1998-09-23 Amersham Pharm Biotech Ab Method of separation of macromolecules
US6638408B1 (en) * 2000-04-03 2003-10-28 The Wistar Institute Method and device for separation of charged molecules by solution isoelectric focusing
US7166202B2 (en) * 2001-07-16 2007-01-23 Protein Forest, Inc. Matrixes, arrays, systems and methods
US6846881B2 (en) * 2002-02-27 2005-01-25 Bio-Rad Laboratories, Inc. Preparation of defect-free polyacrylamide electrophoresis gels in plastic cassettes
US20030226752A1 (en) * 2002-06-05 2003-12-11 Gradipore Limited Method for pH-biased isoelectric trapping separation
AUPS295702A0 (en) * 2002-06-17 2002-07-04 Proteome Systems Intellectual Property Pty Ltd Coated hydrophilic membranes for electrophoresis applications
DE10343334A1 (en) * 2003-09-12 2005-04-14 Proteosys Ag Serial isoelectric focusing of immobilized pH gradients

Also Published As

Publication number Publication date
JP2010526301A (en) 2010-07-29
WO2008137728A1 (en) 2008-11-13
US20080272002A1 (en) 2008-11-06
EP2153218A1 (en) 2010-02-17
CA2686254A1 (en) 2008-11-13
AU2008247532A1 (en) 2008-11-13

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