WO2008117816A1 - Super-sensitive measurement method for protein or nucleic acid - Google Patents

Super-sensitive measurement method for protein or nucleic acid Download PDF

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Publication number
WO2008117816A1
WO2008117816A1 PCT/JP2008/055664 JP2008055664W WO2008117816A1 WO 2008117816 A1 WO2008117816 A1 WO 2008117816A1 JP 2008055664 W JP2008055664 W JP 2008055664W WO 2008117816 A1 WO2008117816 A1 WO 2008117816A1
Authority
WO
WIPO (PCT)
Prior art keywords
super
measurement method
measurement
nad
thio
Prior art date
Application number
PCT/JP2008/055664
Other languages
French (fr)
Japanese (ja)
Inventor
Etsuro Ito
Original Assignee
Etsuro Ito
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Etsuro Ito filed Critical Etsuro Ito
Priority to JP2009506355A priority Critical patent/JP5500985B2/en
Publication of WO2008117816A1 publication Critical patent/WO2008117816A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G01N33/5735—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes co-enzymes or co-factors, e.g. NAD, ATP
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Disclosed is a super-sensitive measurement method which can achieve the measurement visually or on a general-purpose measurement apparatus such as an absorption spectrophotometer or a plate reader plate reader. Specifically disclosed is a highly sensitive measurement method which can achieve the measurement visually or on a general-purpose measurement apparatus, and which is characterized by amplifying thio-NAD(P)H (which is a signal substance) exponentially and colorimetrically measuring the amount of thio-NAD(P)H by employing an optimal combination of an enzyme cycling method that employs thio-NAD(P) as a coenzyme with a labeling enzyme and a substrate for the labeling enzyme.
PCT/JP2008/055664 2007-03-28 2008-03-26 Super-sensitive measurement method for protein or nucleic acid WO2008117816A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009506355A JP5500985B2 (en) 2007-03-28 2008-03-26 Ultrasensitive measurement method of protein and nucleic acid

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-085550 2007-03-28
JP2007085550 2007-03-28

Publications (1)

Publication Number Publication Date
WO2008117816A1 true WO2008117816A1 (en) 2008-10-02

Family

ID=39788549

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/055664 WO2008117816A1 (en) 2007-03-28 2008-03-26 Super-sensitive measurement method for protein or nucleic acid

Country Status (2)

Country Link
JP (1) JP5500985B2 (en)
WO (1) WO2008117816A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012128338A1 (en) 2011-03-23 2012-09-27 Ito Etsuro Method and kit for super-high-sensitivity measurement of protein and nucleic acid, and novel enzyme substrate
JP2017176039A (en) * 2016-03-30 2017-10-05 悦朗 伊藤 Ultrahigh-sensitive assay for protein and nucleic acid

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108872205A (en) * 2018-06-19 2018-11-23 深圳上泰生物工程有限公司 The application of object detection method of content and detection reagent in the detection method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10225300A (en) * 1997-02-14 1998-08-25 Asahi Chem Ind Co Ltd Highly sensitive determination of glucose or adp

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1033196A (en) * 1996-07-23 1998-02-10 Unitika Ltd Test piece
JP4093507B2 (en) * 1997-03-10 2008-06-04 旭化成ファーマ株式会社 Buffer composition
AU785111C (en) * 2000-03-23 2007-05-03 Lumigen, Inc. Methods of detecting polynucleotide kinase and its use as a label

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10225300A (en) * 1997-02-14 1998-08-25 Asahi Chem Ind Co Ltd Highly sensitive determination of glucose or adp

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ITO E. ET AL.: "1-ko no Saibo no Naka no 1-ko no Tanpakushitsu no Teiryo o Mezashite", BIOTECHNOLOGY JOURNAL, vol. 6, no. 5, 2006, pages 609 - 612 *
IWAI A. ET AL.: "Thio NAD Cycling ni yoru Androsterone no Kokando Teiryo", THE JAPAN SOCIETY FOR ANALYTICAL CHEMISTRY NENKAI KOEN YOSHISHU, vol. 56, 5 September 2007 (2007-09-05), pages 207 *
KISHI K. ET AL.: "Highly sensitive cholesterol assay with enzymatic cycling applied to measurement of remnant lipoprotein-cholesterol in serum", CLIN. CHEM., vol. 48, no. 5, 2002, pages 737 - 741 *
KOUZUMA T. ET AL.: "An enzymatic cycling method for the measurement of myo-inositol in biological samples", CLIN. CHIM. ACTA, vol. 312, no. 1-2, 2001, pages 143 - 151, XP002329898, DOI: doi:10.1016/S0009-8981(01)00614-3 *
TSUSHIMA K. ET AL.: "Toseki to Carnitine II Ketchu Carnitine no Sokuteiho", THE JAPANESE JOURNAL OF CLINICAL DIALYSIS, vol. 16, no. 2, 2000, pages 167 - 173 *
UEDA S. ET AL.: "Kinetic study of the enzymatic cycling reaction conducted with 3alpha-hydroxysteroid dehydrogenase in the presenence of excessive thio-NAD(+) and NADH", ANAL. BIOCHEM., vol. 332, no. 1, 2004, pages 84 - 89, XP004525468, DOI: doi:10.1016/j.ab.2004.04.035 *
ZHANG G.H. ET AL.: "An enzymatic cycling method for the determination of serum total bile acids with recombinant 3alpha-hydroxysteroid dehydrogenase", BIOCHEM. BIOHPYS. RES. COMMUN., vol. 326, no. 1, 2005, pages 87 - 92, XP004672553, DOI: doi:10.1016/j.bbrc.2004.11.005 *

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012128338A1 (en) 2011-03-23 2012-09-27 Ito Etsuro Method and kit for super-high-sensitivity measurement of protein and nucleic acid, and novel enzyme substrate
JP5265816B2 (en) * 2011-03-23 2013-08-14 悦朗 伊藤 Ultrasensitive measurement methods and kits for proteins and nucleic acids, and novel enzyme substrates
EP2695948A4 (en) * 2011-03-23 2015-04-08 Etsuro Ito Method and kit for super-high-sensitivity measurement of protein and nucleic acid, and novel enzyme substrate
EP2987866A2 (en) 2011-03-23 2016-02-24 Etsuro Ito Method and kit for super-high-sensitivity measurement of protein and nucleic acid, and novel enzyme substrate
EP2987866A3 (en) * 2011-03-23 2016-06-15 Etsuro Ito Method and kit for super-high-sensitivity measurement of protein and nucleic acid, and novel enzyme substrate
US9851309B2 (en) 2011-03-23 2017-12-26 Satoshi Watabe Ultra-high-sensitive assay of protein and nucleic acid and kit, and novel enzyme substrate
EP3296405A1 (en) 2011-03-23 2018-03-21 Etsuro Ito Method and kit for super-high-sensitivity measurement of protein and nucleic acid, and novel enzyme substrate
US11221299B2 (en) 2011-03-23 2022-01-11 Satoshi Watabe Ultra-high-sensitive assay of protein and nucleic acid and kit, and novel enzyme substrate
JP2017176039A (en) * 2016-03-30 2017-10-05 悦朗 伊藤 Ultrahigh-sensitive assay for protein and nucleic acid
WO2017169565A1 (en) * 2016-03-30 2017-10-05 悦朗 伊藤 Method for measuring protein and nucleic acid with ultrahigh sensitivity
US11702684B2 (en) 2016-03-30 2023-07-18 Biophenoma Inc. Method for measuring protein and nucleic acid with ultrahigh sensitivity

Also Published As

Publication number Publication date
JPWO2008117816A1 (en) 2010-07-15
JP5500985B2 (en) 2014-05-21

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