WO2008115054A3 - A method for regulating eukaryotic gene expression at the level of chromatin - Google Patents

A method for regulating eukaryotic gene expression at the level of chromatin Download PDF

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Publication number
WO2008115054A3
WO2008115054A3 PCT/NL2008/050150 NL2008050150W WO2008115054A3 WO 2008115054 A3 WO2008115054 A3 WO 2008115054A3 NL 2008050150 W NL2008050150 W NL 2008050150W WO 2008115054 A3 WO2008115054 A3 WO 2008115054A3
Authority
WO
WIPO (PCT)
Prior art keywords
chromatin
nucleic acid
methods
gene expression
acid construct
Prior art date
Application number
PCT/NL2008/050150
Other languages
French (fr)
Other versions
WO2008115054A2 (en
Inventor
Pernette Jeanine Verschure
Maartje Carolien Brink
Original Assignee
Univ Amsterdam
Pernette Jeanine Verschure
Maartje Carolien Brink
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Amsterdam, Pernette Jeanine Verschure, Maartje Carolien Brink filed Critical Univ Amsterdam
Publication of WO2008115054A2 publication Critical patent/WO2008115054A2/en
Publication of WO2008115054A3 publication Critical patent/WO2008115054A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

The present invention relates to methods for inducing changes in epigenetic gene control in a chromatin domains in eukaryotic cells, and to nucleic acid constructs and host cells for use in such methods. The methods use nucleic acid constructs that are preferablyintegrated at a predetermined chromatin region of the host cell's genome, which nucleic acid construct comprise binding sites for a DNA-binding protein. Proteins that induces or are suspected to induce epigenetic gene control may then be targetedtothe integrated nucleic acid construct by means of fusion to the DNA binding protein. The targeting protein that induces or is suspected to induce epigenetic gene control to the integrated nucleic acid construct then allows to determine its effects on chromatin structure, gene expression, and epigenetic status of the chromatin locus.
PCT/NL2008/050150 2007-03-19 2008-03-17 A method for regulating eukaryotic gene expression at the level of chromatin WO2008115054A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US90707707P 2007-03-19 2007-03-19
US60/907,077 2007-03-19
EP07104423.4 2007-03-19
EP07104423 2007-03-19

Publications (2)

Publication Number Publication Date
WO2008115054A2 WO2008115054A2 (en) 2008-09-25
WO2008115054A3 true WO2008115054A3 (en) 2008-11-27

Family

ID=38236204

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2008/050150 WO2008115054A2 (en) 2007-03-19 2008-03-17 A method for regulating eukaryotic gene expression at the level of chromatin

Country Status (1)

Country Link
WO (1) WO2008115054A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004020630A2 (en) * 2002-08-27 2004-03-11 Washington University Method for gene isolation by cre-trap cloning
WO2004033471A2 (en) * 2002-10-04 2004-04-22 Genencor International, Inc. Glucose transport mutants for production of biomaterial

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004020630A2 (en) * 2002-08-27 2004-03-11 Washington University Method for gene isolation by cre-trap cloning
WO2004033471A2 (en) * 2002-10-04 2004-04-22 Genencor International, Inc. Glucose transport mutants for production of biomaterial

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
CREMAZY ET AL: "Imaging in situ protein-DNA interactions in the cell nucleus using FRET-FLIM", EXPERIMENTAL CELL RESEARCH, SAN DIEGO, CA, US, vol. 309, no. 2, 1 October 2005 (2005-10-01), pages 390 - 396, XP005074656, ISSN: 0014-4827 *
GRONOSTAJSKI R M AND SADOWSKI P D: "Determination of DNA sequences essential for FLP-mediated recombination by a novel method", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 260, 1985, pages 12320 - 12327, XP002444288 *
LACHNER M ET AL.: "Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins", NATURE, vol. 410, March 2001 (2001-03-01), pages 116 - 120, XP002444287 *
MAARTJE C BRINK ET AL: "Truncated HP1 lacking a functional chromodomain induces heterochromatinization upon in vivo targeting", HISTOCHEMISTRY AND CELL BIOLOGY ;, SPRINGER-VERLAG, BE, vol. 125, no. 1-2, 1 January 2006 (2006-01-01), pages 53 - 61, XP019343118, ISSN: 1432-119X *
MATEOS-LANGERAK J ET AL.: "Pericentromeric heterochromatin domains are maintained without accumulation of HP1", MLECULAR BIOLOGY OF THE CELL, vol. 18, April 2007 (2007-04-01), pages 1464 - 1471, XP002444289 *
MATZKE A J M ET AL.: "Use of two-color fluorescence-tagged transgenes to study interphase chromosomes in living plants", PLANT PHYSIOLOGY, vol. 139, December 2005 (2005-12-01), pages 1586 - 1596, XP002444284 *
PETERS A H F ET AL.: "Histone H3 lysine 9 methylation is an epigenetic imprint of facultative heterochromatin", NATURE GENETICS, vol. 30, January 2002 (2002-01-01), pages 77 - 80, XP002444286 *
ROBINETT C C ET AL.: "In vivo localization of DNA sequences and visualisation of large-scale chromatin organisation using Lac operator/repressor recognition", THE JOURNAL OF CELL BIOLOGY, vol. 6, no. Pt2, December 2006 (2006-12-01), pages 1685 - 1700, XP002444283 *
VERSCHURE P J ET AL.: "In vivo HP1 trageting causes large-scale chromatin condensation and enhanced histone lysine methylation", MOLECULAR AND CELLULAR BIOLOGY, vol. 25, no. 11, June 2005 (2005-06-01), pages 4552 - 4564, XP002444282 *
VERSCHURE P J ET AL.: "Step out of the groove: epigenetic gene control systems a engineered transcription factors", ADVANCES IN GENETICS, vol. 56, 2006, pages 163 - 204, XP008081485 *

Also Published As

Publication number Publication date
WO2008115054A2 (en) 2008-09-25

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