WO2008112980A3 - Procédé et système pour l'assemblage de macromolécules et de nanostructures - Google Patents

Procédé et système pour l'assemblage de macromolécules et de nanostructures Download PDF

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Publication number
WO2008112980A3
WO2008112980A3 PCT/US2008/057013 US2008057013W WO2008112980A3 WO 2008112980 A3 WO2008112980 A3 WO 2008112980A3 US 2008057013 W US2008057013 W US 2008057013W WO 2008112980 A3 WO2008112980 A3 WO 2008112980A3
Authority
WO
WIPO (PCT)
Prior art keywords
nanostructures
macromolecules
assembly
template
peptides
Prior art date
Application number
PCT/US2008/057013
Other languages
English (en)
Other versions
WO2008112980A2 (fr
Inventor
Vincent Suzara
Paul Bentley
Original Assignee
Incitor Llc
Vincent Suzara
Paul Bentley
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/936,045 external-priority patent/US20090118140A1/en
Application filed by Incitor Llc, Vincent Suzara, Paul Bentley filed Critical Incitor Llc
Publication of WO2008112980A2 publication Critical patent/WO2008112980A2/fr
Publication of WO2008112980A3 publication Critical patent/WO2008112980A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Nanotechnology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Composite Materials (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • General Physics & Mathematics (AREA)
  • Materials Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un système à base de gabarit permettant l'assemblage de macromolécules et de nanostructures. Le système de gabarit comprend une pluralité de molécules d'ADN simple brin qui sont sensiblement parallèles, sensiblement en ligne à partir d'une extrémité, et sensiblement espacées de façon égale, chaque molécule d'ADN ayant une longueur distinguable et une séquence connue. Le système peut être utilisé pour la synthèse précise, exacte et rentable de peptides, protéines et enzymes.
PCT/US2008/057013 2007-03-15 2008-03-14 Procédé et système pour l'assemblage de macromolécules et de nanostructures WO2008112980A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US91814407P 2007-03-15 2007-03-15
US60/918,144 2007-03-15
US11/936,045 2007-11-06
US11/936,045 US20090118140A1 (en) 2007-03-15 2007-11-06 Method and system for assembly of macromolecules and nanostructures

Publications (2)

Publication Number Publication Date
WO2008112980A2 WO2008112980A2 (fr) 2008-09-18
WO2008112980A3 true WO2008112980A3 (fr) 2008-11-06

Family

ID=39760417

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/057013 WO2008112980A2 (fr) 2007-03-15 2008-03-14 Procédé et système pour l'assemblage de macromolécules et de nanostructures

Country Status (1)

Country Link
WO (1) WO2008112980A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8993714B2 (en) 2007-10-26 2015-03-31 Imiplex Llc Streptavidin macromolecular adaptor and complexes thereof
CN102171326A (zh) * 2008-08-06 2011-08-31 尹斯特有限公司 高密度多维寻址组件的产生
US9102526B2 (en) 2008-08-12 2015-08-11 Imiplex Llc Node polypeptides for nanostructure assembly
US9869851B2 (en) 2008-12-02 2018-01-16 The Research Foundation For The State University Of New York Centrifuge force microscope modules and systems for use in a bucket of a centrifuge
US8491454B2 (en) * 2008-12-02 2013-07-23 President And Fellows Of Harvard College Spinning force apparatus
US9285363B2 (en) 2009-05-11 2016-03-15 Imiplex Llc Method of protein nanostructure fabrication
EP2576074B1 (fr) 2010-06-01 2019-03-06 President and Fellows of Harvard College Appareil pour la mesure de forces centrifuges associées à des molécules
EP3420352B1 (fr) 2016-02-25 2023-08-09 Children's Medical Center Corporation Appareil de filage pour la mesure de caractéristiques associées à des molécules
EP3475444A1 (fr) 2016-06-22 2019-05-01 The Charles Stark Draper Laboratory, Inc. Composition destinée à être utilisée dans la synthèse des chaînes moléculaires
CN111198175B (zh) * 2018-11-19 2022-09-27 中国科学院宁波材料技术与工程研究所 热点可控分布的宏观大面积纳米金棒二维阵列及应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000061775A1 (fr) * 1999-04-08 2000-10-19 Pavel Sergeev Synthese de composes biologiquement actifs dans des cellules
US20050158763A1 (en) * 2003-12-19 2005-07-21 Albena Ivanisevic Aligned long DNA molecules on templates and methods for preparing

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000061775A1 (fr) * 1999-04-08 2000-10-19 Pavel Sergeev Synthese de composes biologiquement actifs dans des cellules
US20050158763A1 (en) * 2003-12-19 2005-07-21 Albena Ivanisevic Aligned long DNA molecules on templates and methods for preparing

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BROCKMAN J.M. ET AL.: "A multistep chemical modification procedure to create DNA arrays on gold surfaces for the study of protein-DNA interactions with surface plasmon resonance imaging", J. AM. CHEM. SOC., vol. 121, no. 35, 1999, pages 8044 - 8051, XP002949098 *
WALDER J.A. ET AL.: "Complementary carrier peptide synthesis: general strategy and implications for prebiotic origin of peptide synthesis", PROC. NATL. ACAD. SCI. USA, vol. 76, no. 1, January 1979 (1979-01-01), pages 51 - 55, XP000857351 *

Also Published As

Publication number Publication date
WO2008112980A2 (fr) 2008-09-18

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