WO2008112032A2 - Solid phase and catalyzed enabled automated isotope dilution and speciated isotope dilution mass spectrometry - Google Patents
Solid phase and catalyzed enabled automated isotope dilution and speciated isotope dilution mass spectrometry Download PDFInfo
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- WO2008112032A2 WO2008112032A2 PCT/US2007/086795 US2007086795W WO2008112032A2 WO 2008112032 A2 WO2008112032 A2 WO 2008112032A2 US 2007086795 W US2007086795 W US 2007086795W WO 2008112032 A2 WO2008112032 A2 WO 2008112032A2
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- G01N1/44—Sample treatment involving radiation, e.g. heat
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
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- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/141111—Diverse hetero atoms in same or different rings [e.g., alkaloids, opiates, etc.]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
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- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/19—Halogen containing
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- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/20—Oxygen containing
- Y10T436/200833—Carbonyl, ether, aldehyde or ketone containing
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/20—Oxygen containing
- Y10T436/203332—Hydroxyl containing
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y10T436/21—Hydrocarbon
- Y10T436/212—Aromatic
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
- Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25625—Dilution
Definitions
- the present invention relates to a method of improving equilibration of enriched isotopes and tagged species and natural isotope species by using solid phase immobilization of isotopically enriched species and equilibration and simultaneous extraction, separation, and/or selection of the species of analytical interest of the isotopes of the natural and the tagged species on the solid phase and improvement of portability of BDMS and SIDMS and other methods of improving efficiency and equilibration and automation.
- EDMS and SIDMS are based on enriched isotope equilibration with the exact species analytes to be measured.
- Patents describing Isotope Dilution Mass Spectrometry (EDMS) and Speciated Isotope Dilution Mass Spectrometry (SIDMS) and the use of equilibrated solutions and these patents are referenced herein - see U.S. Pat. No. 5,414,259 and U.S. PaL No. 6,790,673 Bl and U.S. Pat. No. 6,974,951 Bl, and Pat. No. 5,883,349, and Pat. No. 5,830,417 and 7,005,635 B2, and U.S. Pat.
- a method for the catalyzed equilibration of enriched isotope species and natural isotope species prior to mass spectrometric analysis using solid phase isotope ratio equilibration and measurement is disclosed.
- the bases of this invention are molecular, elemental and speciated, and quantitative and qualitative sample preparation for definitive qualitative and quantitative analyses of the analytes of interest.
- the method improves equilibration by utilizing solid phases which have many advantages over liquid and gas phase through simultaneous equilibration and enables automation of IDMS and SHDMS analysis known in the art.
- the innovation uses solid phases and immobilized enriched isotope reagents, isotopically enriched molecularly manufactured reagents and the process of equilibration on solid and immobilized phases.
- Algorithms are used to determine mathematically concentrations and directly to correct for species shifts without calibration curves being applied to the mass spectrometry data. Time required to equilibrate and separate the analyte is significantly decreased through sample preparation on solid phases as compared to conventional liquid/thermal equilibration and separation protocols.
- Solid phase isotope spiking and equilibration Reagents and products made for solid phase isotope spiking and equilibration are stable over longer periods of time, thus making it possible to do on-site sample preparation and improve on storage and chain of custody problems associated with degradation of reagents and/or samples while in storage or during shipment
- solid phase isotope spiking and equilibration will make handling of reactive and toxic materials safer in field-spiked and equilibrated forms than they are as bulk reagent solutions, by eliminating several sample preparation and manipulation steps.
- sample analyte and isotopically enriched and equilibrated reagent tags are either eluted off for analysis in liquid and/or gas phase or are directly analyzed in solid phase by surface ionization into the mass spectrometer.
- Solid phase isotope spiking and highly rapid equilibration facilitate the ability to design cost-effective, high-throughput, reliable sample preparation and analysis systems involving high levels of automation and miniaturization sub-systems, thereby making it possible to design highly portable, field-deployable, accurate, low-false positive analytical and detection systems.
- Such field deployable systems will be highly useful for environmental forensics, homeland security and homeland defense, industrial regulation compliance, biosciences and clinical research and clinical diagnostic purposes.
- Some of the homeland defense and homeland security applications include multi-point drinking water network monitoring for fugitive agents and air/water/surface analyses in the battlefield for the protection of armed forces. These systems will also be useful for assessing risks of certain diseases in humans as a function of exposure to industrial toxins from the environment and food within the growing field of environmental health. Eventually, such system may turn into tools that will help predict the onset or slow down the progression of certain diseases like autism, some forms of cancer, and immunodegenerative diseases like Alzheimer's, Parkinson's and diabetes. Definitive study using both concepts described above follows infra. BRIEF DESCRIPTION OF THE DRAWING(S)
- Figure 1 Results from oxygenates equilibrated and separated by SPI-SPE as described and analyzed by GC-MS.
- Figure 2 Results on PAG-5 column spiked with indicated oxygenates and equilibrated and separated on the column as described and analyzed by GC-MS.
- Figure 3 Morphine from serum using Agilent Evidex, 6ml, 0.5g of C-13 isotopically IDMS prespiked morephine and equilibrated with sample morphine on the PSI- SPE column.
- Figure 4 1,4-dioxane and 1,4 dichlorethane using isotopicaly enriched PSI-SPE equilibrated with the natural on the column.
- Figure 7 Calibration Std. vs. Calibration cartridge.
- FIG. 9 Microtiter plate (top view), 8x12 (96 wells) format prepared mass spectrometric (IDMS and/or SIDMS) ELISA, with alternating rows of two different kinds of enriched isotopically modified bound antigens.
- Figure 10 Microtiter plate or a surface modified solid phase support (side view), with alternating, discrete rows of two different kinds of enriched isotopically bound antigens.
- One of isotopically enriched antigen is prepared as a part of the solid surface prior to implementing ELISA with the natural isotopic sample. Alternatively, no isotopically preloaded antigens woud be present on the solid phase and both enriched and non-enriched antigens are equilibrated when bound to the antibodies, rapidly during the ELISA.
- Figure 11 Microtiter plate or a surface modified solid phase support (side view), with some percentage of the antibodies having previously bound isotopically enriched antigen. The ELISA measurement is accomplished by measuring the level of binding of the isotopically enriched antigen present in the sample as a function of ratio between these two sets of antigens.
- Figure 12 Microtiter plate or a surface modified solid phase support (side view), using the sandwich system, and dual antibody and antigen analysis using isotopically enriched antigens and meauring the ratios of the two enriched isotope spikes.
- Figure 13 Microtiter plate or a surface modified SELDI plate with natural abundance biomarkers and isotopically enriched biomarkers (side view), arranged in two discrete rows.
- Figure 14 Top view of an 8-well strip used for SELDI or ELISA.
- Figure 15 A solid phase surface modified plate with 16 rows of alternating bound protein biomarkers or nucleotide probes, both enriched and natural, arranged in high density micorarray format. The quantitation is done using multi-variant biormarkers or nucleotides, both isotopically enriched and natural, and applying the IDMS and/or SIDMS direct ratio algorithms.
- Figure 16 Mass spectroscopy readout demonstrates the isotope enriched specific species spiking of blood for methylmercury, ethylmercury, inorganic mercury and metallic mercury.
- Figure 17 Mass spectroscopy readout shows water containing 20 ppm NaN3 and
- Figure 18 Mass spectroscopy readout shows water sample containing 20ppm NaN3 and 20ppm NaNNl 5N by ESI-TOF-MS in negative mode.
- Figure 20 Mass spectroscopy close up of first sodium azide ion of 20 ppm NaN3 spiked with 20 ppm NaNNl 5N in negative mode in negative mode, the all natural peak is at
- the mixed peak is at 108 and the all isotope peak is at 109 and has a 1:2 or 1:3 ratio for quantification.
- Figure 21 Mass spectroscopy readout shows the many simultaneous ratios that are expressed in this set of molecular species and quantification requires multiple equations and multiple ratios for quantification. These collected graphics are of 20 ppm NaN3 and 20 ppm
- Each graph has one more azide peak.
- Figure 22 Mass spectroscopy readout shows a new ratio relationship and multiple peaks between natural Azide Na3(N3)4- (left most peak) and three corresponding isotopic enriched tagged analogue of Azide Na3(N3)4- with varying numbers of Nl 5 isotopes and ratios.
- This figure is the close up of third sodium azide ion of 20 ppm NaN3 spiked with 20 ppm NaNN15N in negative mode, the all natural peak is at 237, the mixed peaks are at 238,
- FIG. 23 Mass spectroscopy close up of fourth sodium azide ion of 20 ppm NaN3 spiked with 20 ppm NaNN15N in negative mode, the all natural peak is at 302, the mixed peaks are at 303, 304, 305 and 306 and the all isotope peak is at 307.
- Figure 24 Mass spectroscopy readout enhanced view of nanoESI-TOF-MS of fourth potassium cyanide species ion of 100 ppm(ug/g) KCN spiked with 100 ppm K 13 C 15 N in positive mode, the all natural peak is at 299, the isotopic enriched potassium cyanide peaks are the peaks at 301, 303 and 305 and the all isotope peak is at 307, each has been annotated with its mix of isotopic and natural carbon and nitrogen.
- Figure 25 Flow chart showing solid phase spiking and equilibrium.
- Figure 26 Flow chart showing methods of enriched isotope spiking and equilibration.
- Figure 27 Isotopic theoretical overlay and measured spectrum of methylmercury, using ESI-TOF-MS at m/z 338 (Methylmercury and cysteine by ESI-TOF)
- species as employed in respect of the sample containing the species which is to be analyzed quantitatively, shall refer to any chemical species, ionic species, molecular species, complex species such as organic species, organometallic species and complex species such as metal containing proteins and hetero and homogeneous carbon species and other species which are adapted to chemical qualitative and quantitative speciated analysis of the present invention.
- sample preparation must precede measurement.
- IDMS and/or SIDMS which includes species specific isotope dilution mass spectrometry - SSIDMS
- the enriched isotope solution may be so dilute that it is not stable or multiple species in the solution may interact with the enriched isotope before use.
- the enriched isotopes may not be safe to ship in concentrations that are stable.
- the IDMS and SIDMS methods remained a tool only for those with high education, skills and experience. The inexperienced and unskilled may not know how properly to extract the species, then spike, equilibrate in order to obtain quantitation.
- This invention describes methods of improving the stability of the enriched isotope spikes, the spiked species of analytical interest, ease of use, reliability of critical procedures, including equilibration and automation, thereby making the practice and use of DDMS and SIDMS possible for general use by minimum skill personnel and address high-throughput needs of commercial laboratories that often need fast, reliable analyses of large numbers of samples.
- solid phase sorbants to separate analyte from matrix has been known since modem chromatography was developed in the mid 19* century.
- solid phase sorbants to equilibrate the enriched stable isotopic species and to separate matrix and analytes thereby enhancing the applicability and usability of EDMS and SEDMS, as disclosed here, has not been done.
- solid phase material with various different properties are used to hold the enriched isotope species and then deliver them for sample preparation, sample storage or safety purposes, to a laboratory or field-site so that the spike is already in the solid phase and not in reagent solution needing manipulation.
- the same solid phase used for holding the enriched isotope species can also be used to extract the analyte species.
- Solid phases for use herein are selected from the group of ion exchange, adsorption medium, solid phase extraction resins, resin bonded solid phase, surface-modified filters, dual-state liquids used in immobilized liquid extraction (ILE) and fibers such as solid phase microextraction (SPME).
- the isotope enriched molecular or ionic species are stabilized or captured or held, chemically or physically, on the solid phase, chromatography or extraction material.
- the sample containing the species of analytical interest is then added with the natural sample material species of interest and is retained by the appropriate mechanism of this media used to hold the species on the media. This process can be reversed with both species, enriched and natural, eluted in equilibrated relationship.
- the ionization methods would be specific for a solution or gas phase such as electrospray ionization (ESI) or nano-ESI, or atmospheric pressure chemical ionization (APCI) or electron impact (EI) or inductively coupled plasma (ICP) or microwave induced plasma (MIP) and other ionization methods.
- ESI electrospray ionization
- APCI atmospheric pressure chemical ionization
- EI electron impact
- ICP inductively coupled plasma
- MIP microwave induced plasma
- This invention speeds up equilibration in the eluted liquid solution by removing the matrix that may prolong or inhibit or prevent equilibration and placing it on both analytes on the solid phase medium that produces an equilibrated state for elution simultaneously as both species having the same chemistry and affinity being the same molecules but with different isotopic ratios.
- One embodiment permits field or close-proximity use as even for small quantities and concentrations which otherwise would be too dilute to be shipped or be unstable without the solid phase support material.
- Another embodiment enables the sample species now equilibrated on the solid phase to be shipped or transported to the analysis site as enriched isotope and natural equilibrated analytes forms on the solid phase support material.
- the analytes now equilibrated, both enriched and neutral, are eluted at some time in the future or stored for later analysis as archives. This embodiment produces stable archived equilibrated spike and natural sample that may be shipped, stored or archived.
- Another embodiment makes use of the solid phase with the equilibrated analytes and induces ionization directly by surface ionizing the enriched and natural analytes of interest.
- Ionization methods for surface ionization are some of the following, such as matrix assisted laser desorption ionization (MALDI) or desorption electrospray ionization DESI or laser ablation (LA) or enzyme-linked immunosorbent assay (ELISA) or immunochemical analysis (ICA) or surface-enhanced laser desorption ionization (SELDI).
- a method of speeding up equilibration of species-in-solution prior to solid phase separation and high-throughput automation is microwave equilibration acceleration by means of microwave-enhanced chemical methods as opposed to thermal conduction and convection methods.
- the microwave energy selected from the microwave region such as 2450 MHz produces molecular rotation and ionic conductance of all ions and permanent dipoles that enable faster desorption from surfaces and enhance the ionic and molecular equilibration of natural species and isotopically enriched specie analogues which are implemented during the required sample preparation steps such as extraction and decomposition.
- This simultaneous extraction and equilibration can be combined with the equilibration steps on solid phase extraction (described above) to enhance both processes.
- the analytical cycle time can be reduced from 24 hours to less man 600 seconds.
- This combined, simultaneous extraction and equilibration step enables ultra fast reactions that are prerequisite specifications for automation of high-throughput applications in hospital, clinical and commercial laboratories, and for near real time applications in homeland security and homeland defense settings.
- the primary methods disclosed here are pre-absorbed solid phase immobilization of enriched isotope tags, isotopically enriched specie-analogues and natural abundance specie- analogues that are used rapidly to equilibrate species of analytical interest, and microwave- enhanced chemistry significantly accelerated equilibration of isotopic species in solution, or gas form.
- IDMS and SIDMS methods rely on the measurement of isotope ratios, so problems associated with calibration curves, instrument stability and detector signal drift are negated. The key step, therefore, in these two isotope dilution procedures is the equilibration of the isotopically enriched spike and the analyte present within the sample.
- the spiked (isotopically tagged or enriched specie-analogue) material acts as an ideal standard, because only isotope ratios are measured and no external calibration is necessary. This ensures consistently accurate, reproducible measurement of the target analyte.
- the role of the spiked material as an ideal standard for IDMS and SIDMS also negates problems associated with instrumental drift and matrix effects during mass spectrometric detection, since all the isotopes from the species would suffer from these effects in an identical way. See Ruiz Encinar, J.; Rodriguez-Gonzalez, P.; Garcia-Alonso, J.I.; Sanz-Medel, A. Trenes in Analytical Chemistry, 2003, 22(2), 108-114.
- Rodriguez-Gonzalez, P. et. al. have studied different extraction methods, such as microwave assisted extraction, mechanical shaking, alkaline hydrolysis with tetramethylammonium hydroxide (TMAH) and enzymatic digestion for butyltin compounds from biological materials. They have observed extensive degradation of species and lack of equilibration with TMAH and enzymatic digestion. It has been reported that the microwave assisted extraction using acetic acid-methanol mixture produced the best results in terms of low degradation and rapid isotope equilibration and quantitative recoveries. They have also reported in their study that the required complete isotope equilibration was achieved only after the naturally occurring organotin compounds were completely released to the solution from the solid matrix. See Rodriguez-Gonzalez, P.; Garcia Alonso, JJ. ; Sanz-Medel, A. J. Anal. Atom. Spectrom. 2004, 19, 767-772.
- TMAH tetramethylammonium hydroxide
- VaUders et. al. have studied the degree of isotopic equilibration of carbon and oxygen isotopes in a mixture of carbon dioxide gas in the gas phase inside the mass spectrometer during the ratio measurements. See Valkiers, S.; Varlam, M.; Rube, K.; Berglund, M.; Taylor, P.; Wang, J.; Milton, M.; De Bievre, P. International Journal of Mass Spectrometry, 2007, 263, 195-203.
- a method for the catalyzed equilibration of enriched isotope species and natural isotope species prior to mass spectrometric analysis using solid phase isotope ratio equilibration and measurement is disclosed.
- the bases of this invention are molecular, elemental and speciated, and quantitative and qualitative sample preparation for definitive qualitative and quantitative analyses of the analytes of interest.
- the method improves equilibration by utilizing solid phases which have many advantages over liquid and gas phase through simultaneous equilibration and enables automation of IDMS and SIDMS analysis known in the art
- the innovation uses solid phases and immobilized enriched isotope reagents, isotopically enriched molecularly manufactured reagents and the process of equilibration on solid and immobilized phases.
- Algorithms are used to determine mathematically concentrations and directly to correct for species shifts without calibration curves being applied to the mass spectrometric data. Time required to equilibrate and separate the analyte is significantly decreased through sample preparation on solid phases as compared to conventional liquid/thermal equilibration and separation protocols.
- Reagents and products made for solid phase isotope spiking and equilibration are stable over longer periods of time, thus making it possible to do on-site sample preparation and improve on storage and chain of custody problems associated with degradation of reagents and/or samples while in storage or during shipment.
- solid phase isotope spiking and equilibration will make handling of reactive and toxic materials safer in field-spiked and equilibrated forms than they are as bulk reagent solutions, by eliminating several sample preparation and manipulation steps.
- sample analyte and isotopically enriched and equilibrated reagent tags are either eluted off for analysis in liquid and/or gas phase analysis or are directly analyzed in solid phase by surface ionization into the mass spectrometer.
- Solid phase isotope spiking and highly rapid equilibration facilitate the ability to design cost- effective, high-throughput, reliable sample preparation and analysis systems involving high levels of automation and miniaturization sub-systems, thereby making it possible to design highly portable, field-deployable, accurate, low-false positive analytical and detection systems.
- Such field deployable systems will be highly useful for environmental forensics, homeland security and homeland defense, industrial regulation compliance, biosiences and clinical research and clinical diagnostic purposes.
- Some of the homeland defense and homeland security applications include multi-point drinking water network monitoring for fugitive agents and air/water/surface analyses in the battlefield for the protection of armed forces. These systems will also be useful for assessing risks of certain diseases in humans as a function of exposure to industrial toxins from the environment and food within the growing field of environmental health. Eventually, such system may turn into tools that will help predict the onset or slow down the progression of certain diseases like autism, some forms of cancer, and immunodegenerative diseases like Alzheimer's, Parkinson's and diabetes. Definitive study using both concepts described above is follows infra.
- the ICP-MS produces results with a maximum precision (i.e., complex matrices) in the range of 5 to 10%.
- the main problems associated with external calibration are: stability of analyte in solution; accuracy in sample preparation; purity of calibration standards; choice of internal standard; improper instrumental setup; total dissolved solids; non-spectral interferences; matrix matching; standard addition; sample introduction; chromatographic separation; instrument drift with time; nebulization efficiency; droplet size; physical properties of solution; acid content in the solution; analyst's lack of knowledge/training; background correction; mass bias; deadtime; and isobaric and polyatomic interferences. See Vicki, B. Preparation of Calibration Curves: A guide to best practice, LGC, September 2003.
- the standard addition technique is used when the matrix is quite variable and/or when an internal standard that corrects for plasma related effects could not be found.
- the standard addition technique offers better possible solution to matrix interferences through plasma related effects, it requires a linear response. It is therefore very important to work within the linear range for each analyte. See Bonnefoy, C. et al, Anal. Bianal. Chem. 2005, 383, 167-173; Melaku, S. et al, Can. J. Anal. Sic. Spectres., 2004, 49(6), 374-384; Panayot, K. et al, Spectrochim. Acta, Part B, 2006, 61, 50-57.
- Protein biomarkers have had tremendous impact in research and on clinical management of human disease, especially cancer.
- proteomics and genomics have enabled hundreds of biomarkers to be identified in a single discovery effort.
- a well functioning enzyme-linked immunosorbent assay can be used at high throughput with extraordinary sensitivity and specificity for quantifying the target analyte.
- ELISA at the present time is based on colorimetric and fluorescent readers for quantification and is being compared to chromatography and mass spectrometry but has not been combined. See Whiteaker, J.R.; Zhao, Lei; Zhang, H. Y.; Feng, L.C.; Piening, B.D.; Anderson, L.; Paulovich, A.G. Analytical Biochemistry, 2007, 362, 44-54.
- Martens-Lobenhoffer J. et. al., has evaluated the measurement of asymmetric dimethylarginine (ADMA) concentrations in human plasma and serum samples using liquid chromatography mass spectrometry (LC-MS) and compared the results with those obtained from the standard colorimetric ELISA technique. It is reported in this article that the ELISA has produced higher values than the LC-MS, and concluded that the ELISA is matrix dependent. They also concluded that the ELISA overestimated the ADMA concentrations in plasma by a factor of 2. see Martens-Lobenhoffer, J.; Westphal, S.; Awiszus, F.; Bode- Boger, S.M.; Luley, C.
- the two methods produced similar results on powdered milk samples. They have also reported that the ELISA method overestimated the CML concentrations in certain samples with high fat content, such as meat products, and fried foods for which no or low CML levels were detected by GC-MS or HPLC. There might be unspecific interferences of the lipid matrix with the ELISA. See Charissou, A.; Ait- Ameur, L.; Birlouez-Aragon, I. J. Chromatogr. A, 2007, 1140, 189-194. Similar findings were also reported by Scholl, P.F.
- Wolthers B.G. et. al., has evaluated the ELISA method for the determination of metanephrine (MA) and normetanephrine (NMA) from human urine and compared the result with those obtained from GC-MS using internal standard and. calibration curve but referring to the GC-MS internal standard as IDMS analysis. They have concluded that the ELISA method is capable in the quantification of urinary MA and thus can be successfully used to establish the diagnosis of pheochromocytoma, and also recommended that this simple ELISA method can be executed in any clinical laboratory and hoped that in time it may replace the currently in practice, more complicated, chromatographic techniques.
- MA metanephrine
- NMA normetanephrine
- the sample was both extracted and spike-equilibrated with microwave energy, then compared with the presorbed solid phase species isotopic spiking and found that both provided accurate data.
- the example described here involves the processing of two sets of the IAEA-085 reference material human hair as identical samples. Table 1 data from the implementation of the conventional EPA Method 6800, SIDMS, where the sample was spiked with isotopically enriched methylmercury before extraction with microwave energy.
- Table 2 is from the same standard sample (IAEA-085) and was subjected to the new method of first having the methylmercury extracted using EPA microwave extraction method, EPA Method 3200, without being spiked, and then added to the SCF column where the spike was equilibrated in a solid phase packed as a bed of flow-through medium in a column rather than in solution, as done in current state-of-the-art chemical procedures.
- the pre-spiked solid phase material or enriched specie-analogues bound to solid phase material is an effective method to implement both EDMS and SIDMS.
- sample collection sample collection
- chain of custody sample containment, shipment and storage that minimize or eliminate loss of analyte
- laboratory analysis sample preparation.
- Advances in analytical chemistry have led to the development of instruments with detection limits as low as one part per trillion which is well below the stability of aqueous standards.
- sample preparation remains one of the slower, labor-intensive and often serially-implemented laboratory processes.
- the current practice of obtaining and processing of large volumes of sample for each analysis is laborious, time-consuming, costly and unfeasible for rapid transportation and high-throughput analysis.
- Solid Phase Extraction (SPE) cartridges are packed with stable isotopically tagged speci-analogues presorbed on solid phase material.
- the modified SPE cartridges are designed for onsite extraction, and prepared specifically for palicular analyte groups. Prepared extraction columns are created with the proper sorbent, bed depth, calibrated reservoir volume, and isotopically labeled analogues. Field extraction is enabled and simplified, requiring minimal sample handling.
- the SPE cartridge After field extraction, the SPE cartridge is shipped to the laboratory, where the isotopic standards and analytes of interest are desorbed via elution by an organic solvent.
- the analytes and isotopic standards are immobilized on the solid phase media while in transit and storage, without the matrix, where they may be less susceptible to modification and degradation.
- Analysis is performed with GC-MS using conventional internal standard quantitation, or isotope dilution quantitation.
- the simplicity of this sampling and extraction protocol enables a streamlined approach to environmental analysis, extending stability, and improving the precision, accuracy, and ruggedness of field sampling and analysis. An enhanced level of quality assurance and quality control is gained in the overall process.
- On-site SPE is an extraction method that can be performed in the field and in the laboratory by less experienced personnel.
- the method can be performed without extensive training, using inexpensive, relatively simple, manual or automated extraction with pre-spiked SPE cartridges.
- the water sample instead of field personnel placing the water sample to be analyzed in a container to be shipped to the analytical lab, the water sample is placed into a calibrated, sample reservoir attached to a SPE cartridge and isotopically equilibrated in the field on the solid phase cartridge. After the sample has been added, it is passed through the SPE media, using either positive pressure, or vacuum.
- the organic analytes and species of interest are removed from the water due to relatively strong intermolecular forces of attraction between the sorbent media and the organic molecules.
- the water that has essentially been stripped of the organic analytes pass through the SPE cartridge.
- the analytes and isotopic standards are immobilized on the solid phase media without the water matrix, and are therefore less susceptible to modification and degradation that can occur during the period of time when water samples are shipped to the lab, or during storage.
- This invention is a simplified and streamlined sample preparation method that removes several levels of manipulation, each potentially introducing errors due to loss of analyte, incomplete chemical manipulation steps and bias. Further, the invention saves time, money, and enables automation. Examples of water extraction on pre-adsorbed enriched isotopes in solid phase C-18 cartridges
- PSI-SPE pre-spiked stable isotope solid phase extraction
- GC-MS GC-MS
- PSI-SPE was performed on the compound classes; oxygenates, and PAG-5, and on the compounds morphine, 1.4-dioxane, and 1,2-dichloroethane. These compound classes are typical for environmental forensic, environmental health and toxicological measurements.
- the overall results using PSI were found to be statistically indistinguishable to those obtained using conventional laboratory SPE, and certainly within the acceptable ranges for the applicable EPA method specifications.
- Oxygenates are a list of small, polar compounds that are frequently found in gasoline, added to the distillate to enhance combustion process, as an "anti-knock" agent.
- This list of compounds includes: tert-butanol (TBA), methyl-t-butyl ether (MTBE), ethyl-t- butyl ether (ETBE), diisopropyl ether (DUPE), and t-amylmethyl ether (TAME).
- TAA tert-butanol
- MTBE methyl-t-butyl ether
- ETBE ethyl-t- butyl ether
- DUPE diisopropyl ether
- TAME t-amylmethyl ether
- MTBE has been shown to degrade into TBA during shipping. This can affect extraction efficiencies and degradation during extraction of oxygenates.
- the problem has been overcome using PSI-SPE method.
- Samples of water spiked with oxygenates have been extracted with excellent results, as seen in Figure 2.
- the PAG-5 list of analytes is a group of toxins and pollutants that must be analyzed by many environmental enforcement agencies when closing or monitoring a gasoline- contaminated site.
- This complex list contains the oxygenate MTBE, the volatile monocyclic aromatics benzene, ethylbenzene, and xylenes, as well as three semivolatile analytes; naphthalene, fluorene, and phenanthrene.
- 1,4-dioxane Another example of small, polar molecule is 1,4-dioxane.
- This compound is commonly used as a disinfecting agent. While it has not been shown to degrade during extraction or analysis, it is, like oxygenates, very hard to extract from water using conventional means. SPE can, however, be employed in a fashion similar to oxygenate analysis, with very good results.
- TTCE Tetrachloroethane
- 1 ,2-DCA 1 ,2-dichloroethane
- 1,2-DCA 1 ,2-dichloroethane
- 1,2-DCA is structurally very different from the non-polar aromatic BTEX, or the very polar oxygenates and dioxane.
- 1,2-DCA has an intermediate polarity, and is a halogenated compound (see Figure 4). Under SPE conditions identical for that of the extraction of the afore mentioned analytes, favorable results can also be obtained for 1,2- DCA.
- a problem that can occur when using conventional sampling and analysis methodologies is that of compound losses during transport via microbial or chemical degradation. Many microbes are capable of consuming pollutants as a food source. If this occurs during transportation of the sample, the analyte losses cannot be determined, and the accuracy of the measure is compromised.
- a study was performed to evaluate if degradation could take place on the SPE cartridge after the sample has been extracted and equilibrated on the resin. Natural water samples were taken from a monitoring well, analyzed and found to contain a number of contaminants. The sample was extracted and analyzed via SPE and PSI- SPE at day 0 (day 0 indicating 0 days had elapsed between the sampling event and the extraction/analysis).
- a calibration cartridge is an SPE cartridge that has been prepared in the same manner as the sample cartridge, with internal standards presorbed on the solid phase material.
- the response factor is the ratio of the area of a compound to the area of its isotopically labeled analog, a given concentration. If the response for both of these analytes is identical, throughout the extraction and analysis processes, the area counts should be identical, and the response factor therefore equal to 1. In practice, however, the responses will vary slightly for a compound and its isotopically altered analog, due mainly to non- perfect mass measurements when preparing the standards, the response factors, in reality, are very close to 1.0. If the response factor for a compound is one, or sufficiently close to assume a value of 1.0, quantitation and even analysis becomes much easier. In fact, if the response factor can always assumed to be 1.0, there is no longer any need to prepare and run a calibration standard.
- PSI-SPE As a means to allow for automation is an attractive goal which will influence the design of automation features in future instrument systems designs.
- the following is a sampling of a few areas where automation in one form or another can be employed using PSI-SPE.
- PSI-SPE For environmental forensic and environmental health monitoring, there are a number of opportunities to apply this technology. Monitoring drinking water, fresh water or wells, located on sites that have been determined to be contaminated or to monitor for contamination continuously with routine sampling are all opportunities to automate the sampling due to the long term stability of these solid phase pre- spiked materials.
- PSI-SPE could be employed and/or could be duplicated on a manifold system, where prepared cartridges are mounted.
- sampling could be regularly performed at a predetermined time. Flow from a sampling stream could be diverted through the cartridge, for the appropriate amount of time, and then the flow of sample replaced with a stream of dry air, to remove residual water after PSI-SPE sampling component. With the extraction complete, the samples are stable for long periods of time or can be immediately removed and the sample cartridge analyzed on site or transported or even mailed to an analytical lab. Duplicate samples can be distributed for a variety of MS analysis and also archived for long- term Quality Control (QA) and validation. Food, beverage and consumable analysis
- QA Quality Control
- PSI-SPE could cost- effectively be performed through the use of automated manifold systems as a stand alone device or a front-end module of a completely integrated, automated analytical measurements system.
- a time study was performed to provide an example of the time savings that could be realized using pre-spiked stable isotope solid phase extraction (PSI-SPE).
- PSI-SPE stable isotope solid phase extraction
- the PSI-SPE results in significant time-savings. Processing the samples from field to finished result was performed in 37% of the conventional methodology. Quality of data was superior to those obtained by means of the conventional protocols.
- Analytes of interest were detected in monitoring well #9 (MW-9), monitoring well #16 (MW- 16), and monitoring well #19 (MW-19). The identities of the analytes, as well as the determined values were all in close agreement between the two methods.
- Presorbed Stable Isotope - Solid Phase Extraction has been shown, by reducing it to practice, to be an effective method of sample extraction and equilibration.
- PSI-SPE will remove many sources of error inherent in current laboratory extraction methods and be the basis of automation that will result in the design of novel, efficient, reliable, rapid sample preparation devices and systems.
- Mammalian immune reaction starts with the recognition of a compound that the immune system cannot recognize (antigen) by a particular special groups of cells (B cells). Then, the immune system starts producing antigen-specific B cells that produce specialized proteins (antibodies) with specific properties to bind to the antigens. Once bound, to the antigen, the B cells then facilitate a series of reactions that aims to eliminate the antigen, as soon as possible.
- Immunodiagnostic assays use this host defense proteins (antibodies) to detect foreign substances, such as viral antigens, directly in the person's blood. Immunoassays are a group of highly specific protein binding assays in which the antigen recognition properties of antibodies are utilized.
- the most popular immunoassay used today is the ELISA (Enzyme Linked ImmunoSorbant Assay) method.
- the key to all ELISA systems is the use of antibodies.
- Antibodies are produced in animals in response to antigenic stimuli.
- Antibodies are specific biochemicals that bind to the antigens used to detect particular antigens used for their production. Thus, they can be used to detect particular antigens if binding can be demonstrated.
- specific antibodies can be measured by the use of defined antigens, and this forms the basis of many assays in the immunochemical research and diagnostic biology fields.
- the basis of quantitation relies on the enzyme-generated signal to be proportional to, or in linear relationship with the concentration of antigen.
- Advantages of ELISA are simplicity, ease-of-reading (by eye or a device), rapidity, sensitivity, commercial availability of reagents, kits and instruments, adaptability, analyst and laboratory safety, safe disposal, relatively easy standardization and quantitation.
- One key advantage is that, for achieving definitive quantitation and reproducibility, removal of the entire modified solid phase surface/matrix (containing the isotopically enriched tag) and analyte are not required. Under EDMS and/or SEDMS conditions, any portion of the modified solid phase surface/matrix (containing the isotopically enriched tag) will permit quantification based on isotopic ratios and not calibration curves. Achieving quantification without the calibration curve is unique for EDMS and SEDMS, as other forms of MALDI or LA require quantifiable and reproducible removal of the surface to produce calibration curves. Here, any portion of the equilibrated surface yields accurate quantification and precludes errors normally associated with both mass spectrometry quantification and ELISA quantification.
- ELISA fluorometric quantification
- PCBs polychlorinated biphenyls
- BTX benzene, toluene, xylene
- Some of the challenges that restrict widespread use of ELISA in the environmental A field are detection limits, calibration curve errors and matrix interferences.
- ELISAs can be carried on in several formats on a variety of solid support material produced in different shapes and packages.
- the most popular ELISAs utilize plastic microtiter plates in an 8x12 well format as the solid phase.
- an ELISA test can be done in each of the 92 individual wells in a microtiter plate (see Figure 9).
- Dilution Linearity This is closely related with the next step, recovery rate. When signal (expressed as peak area, peak area or intensity) vs dilution factors plotted on an x-y chart, it must produce a straight line.
- Recovery Rate This is the percent of the concerned material observed after the assaying when a known quantity of the concerned materials is added into the assay reaction.
- the recovery rates should be within 10% for the clinical routine work.
- Intraassay and interassay variation The intra-assay means values in one
- C-ELISA Competition
- I-ELISA Inhibition
- Indirect ELISA Antibodies form a particular biological species react with antigen attached to the solid phase. Any bound antibodies are detected by the addition of an antispecies antiserum labeled with enzyme. This is widely used in clinical diagnosis.
- Sandwich ELISA This system involves the antibody or the capture antigen attached to a solid phase material.
- Direct sandwich ELISA If it is a direct sandwich assay, the detecting antibody is labeled with enzyme. The antigen is detected using serum specific for the antigen. The detecting antibody is labeled with enzyme. The capture antibody and the detecting antibody can be same serum or serum from different animals of the same species or from different species. The antigen for a direct sandwich assay must have at least two antigenic sites.
- Indirect sandwich ELISA If the system is an indirect sandwich assay, the antigen is captured by a solid phase bound antibody. Antigen is then detected using antibodies from another species. This, in turn, is bound by an antispecies conjugate. Thus, the species of serum for the coating and detecting antibodies must be different; the antispecies conjugate cannot react with the coating antibodies.
- HRP horseradish peroxidase
- AP alkaline phosphatase
- Other enzymes such as ⁇ -galactosidase, acetylcholinesterase and catalase have also been used, but limited substrate options, limited their widespread applications.
- a detection enzyme may be linked directly to the primary antibody or introduced through a secondary antibody that recognizes the primary antibody. It may also be linked to a protein such as streptavidin if the primary antibody is biotm labeled. The choice of substrate depends upon the necessary sensitivity level of the detection and the instrumentation available for detection (spectrophotometer, fluorometer or luminometer).
- biotinylation is the most popular one because of the simplicity of the labeling and spectrometric measurement and the high specificity and selectivity of avidin (a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia) with the small vitamin, biotin.
- avidin a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia
- Avidin-biotin reaction is the most useful tool in assay systems designed to detect and target biological analytes.
- the extraordinary affinity of avidin for biotin allows biotin-containing molecules in a complex mixture to be discretely bound with avidin conjugates.
- mass spectrometers and isotopic measurement techniques are highly desirable as potential immunoassay detection systems because of their inherent high sensitivity and significantly lower interferences, they have not been successfully used as the definitive quantitation detector for ELISAs because of a number of obstacles. Chief among them are the high cost of the mass spectrometers, instability of the mass spectrometer detector signal and lack of expertise among the biological scientists about isotopic analysis and relatively recent popularity of mass spectrometers in the field of bioanalytical measurements.
- enriched isotopic tags or isotopically enriched synthetic peptides mimicking the antigenic sites or antibody are feasible for ELISA type of assays when these peptides are placed in discrete sample spots arranged as rows of bound-antigens or immobilized antibodies in microarray plates.
- the microarray plates are then be processed and introduced to the mass spectrometer for liquid and/or gas ionization and definitively quantitative mass spectrometric analysis.
- Whole cells, live or attenuated, with isotopic tags incorporated through nutrients in the media during fermentation or through a chemical isotopic tagging process can be used when the cells with the can immobilized on solid phase matrices are immobilized and provide as antigenic lattices.
- Such cells are enclosed in specially designed multi-array chips with discrete sample holding sites having the ability to keep the cells bound and viable for the duration of the analysis cycle in the mass spectrometer.
- the isotopic analysis methods using the principals of EDMS and/or SIDMS and the inventions described herein are used for capture and analysis of biomolecules, by immobilizing ligands, such as lectins, polysaccharides, nucleotides, biomolecule and/or chemical toxins that can be isolated from a natural source or synthesized as functional analogues, with affinity for other biomolecules. Enriched isotopes can be used for visualization of each of the thousands nucleotide probes that are immobilized on the gene- chips microarrays.
- ligands such as lectins, polysaccharides, nucleotides, biomolecule and/or chemical toxins that can be isolated from a natural source or synthesized as functional analogues, with affinity for other biomolecules.
- Enriched isotopes can be used for visualization of each of the thousands nucleotide probes that are immobilized on the gene- chips microarrays.
- SELDI Surface Enchanced Laser Desorption Ionization
- Enriched isotope tags overcome these limitations by providing a means to measure isotopically tagged biomarker ratios on a SELDI protein chips, vastly improving quantitation and reproducibility through EDMS and/or SIDMS quantitation. See Figures 13, 14 and 15. Direct Tissue Profiling Using IDMS and/or SIDMS
- New molecular profiling technologies aid in analysis of small pathologic samples obtained by minimally invasive biopsy, enabling the discovery of key biomarkers synergistic with anatomopathologic analysis related to prognosis, therapeutic response, and innovative target validation.
- proteomic analysis at the histologic level in healthy and pathologic settings is a major issue in the field of clinical proteomics.
- Direct tissue proteomic analysis (DTPA) is an original application of SELDI-MS technology that can expand the use of clinical proteomics as a complement to the anatomopathological diagnosis.
- the DPTA method offers unique high-throughput characteristics that can be used for biomarker discovery in large cohorts of patients.
- the DPTA approach has been used for classification of diseases such as lung carcinoma and brain tumors thus enhancing anatomopathological diagnostic techniques.
- the DPTA is a recently developed fast, sensitive technique that opens the door to new perspectives in clinical proteomics.
- Current developments in this area, addressing the needs for definitive quantitation, reproducibility and sensitivity are achieved through the use of enriched isotope tags and application of the principles of EDMS and/or SIDMS introduced as solid phase surface modified or unmodified media.
- Enriched isotopic analogues of analytes of interest are created from primary enriched isotopes or are purchased or synthesized in the laboratory.
- the application of EDMS and SIDMS coupled to various methods of ionization such as ESI, nanoESI, nanochipESI, DESI, MALDI, LA, SELDI, APCI, ICP, GC-ICP, GC-MS are embraced by the present invention.
- ESI, nanoESI, nanochipESI, DESI, MALDI, LA, SELDI, APCI, ICP, GC-ICP, GC-MS are embraced by the present invention.
- GC-ICP-MS and nanoelectrospray Time of Flight mass spectrometry are shown.
- nanochipESI-TOF-MS time of flight mass spectrometry
- the first example of a measurement that cannot be quantified by calibration curve and that must be accomplished by SEDMS measurement is a blood sample with methylmercury and inorganic mercury and also spiked for ethylmercury. This sample was separated and ionized after equilibration with separate isotopic analogues of inorganic, ethyl and methyl mercury.
- inorganic mercury Hg +2
- inorganic mercury Hg +2
- CHjHg + methylmercury
- C 2 HsHg + ethylmercury
- metallic mercury species Hg 0
- Figure 16 below demonstrates that a calibration curve would be difficult if not impossible to use as different amounts of both inorganic, methyl and/or ethyl mercury in human blood or urine would produce different amounts of all four species (inorganic, methyl, ethyl and metallic mercury) in different proportions and no calibration curve could be established for unknown individual samples. Only mathematical deconvolution by SIDMS methods can permit quantitation in such cases. As in the example above for human hair and the 6 separate transformations that are described there in this example there are at least 4 more that would have to be evaluated and corrected for as it is not only the formation of the fourth species of metallic mercury here but also the contribution to this fourth species by the transformation of inorganic mercury, methylmercury, and ethylmercury.
- IDMS, SBDMS and direct species algorithms depend on the species generated and only direct mathematical algorithms newly derived for IDMS and SIDMS analysis can be used for quantification of dynamic systems.
- the enriched stable isotope spike must have a different isotopic composition from the sample but the same chemical form and chemistry of the analyte(s) of interest.
- the matrix composition of the actual sample and the normal standards are rarely the same and any difference in the composition of other elements makes for different isotopic analogues being expressed in soft ionization methods such as ESI, nanoESI, nanochipESL MALDI, SELDI, and APCI. Thus there cannot be any calibration curves that will represent the sample accurately.
- An example is presented here for sodium azide and potassium cyanide.
- the spike is prepared either in aqueous and/or acid and/or in organic solvent solutions, or fixed to a surface by adsorption, ion exchange or bonding. Calibration by establishing a calibration curve can not be accomplished in soft ionization methods such as ESI, nanoESI, DESL, MALDI and SELDI or in hard ionizations such as ICP-MS as in the above mercury species example.
- soft ionization methods the ions being measured and quantified is dependent on the matrix with the analyte in the real sample and can not be simulated by a standard or even a standard attempting to matrix match.
- the ion is a product of the matrix interactions simultaneously.
- the a ⁇ alyte may have many representative molecular ions and ion species that are represented in the mass spectrometer.
- Azide will be used as an example to show several representations based on the matrix and sample conditions. Each representation requires different mathematical quantification using IDMS and SDDMS directly.
- Soft ionization does not produce molecular ions independent of the matrix but incorporate the matrix and environment to change the concentration of which molecular ions and isotopically enriched analogue ions are expressed. Examples are provided below. Calibration curves and the use of standard IDMS and SEDMS equations can not be used to quantify but quantification is possible only by using new mathematical algorithm protocols for these isotopic species from soft ionization. As you can see the standard IDMS equations do not account for multiple expression and simultaneous and different isotopic ratios for quantification.
- Equation- 1 The general mathematical equation for the quantitative determination of the isotope ratio in IDMS is shown in Equation- 1 and the direct algorithm for a mathematical solution to determine the concentration of an unknown sample is a rearrangement of this equation and is shown in Equation-2.
- Equation-2 The individual components of this direct mathematical solution is presented below in Equation-2.
- a 5 atom fraction of altered isotope A in the spike (enriched)
- a x atom fraction of isotope A in sample (natural)
- B s atom fraction of altered isotope B in the spike (enriched)
- M x average atomic mass of the species in the sample
- Ms average atomic mass of the species in the spike
- R m measured isotope ratio of isotope A to isotope B (enriched/natural) ⁇
- Soft ionization methods such as ESI, nanoESI, MALDI, APCI, and EI are examples of some of these molecular soft ionization sources that are most susceptible to complex molecular ions that can not be duplicated in standard solutions without the matrix.
- Dynamic species and species that are determined by the sample matrix itself using soft ionization methods require very different mathematical treatment than hard ionization methods such as ICP-MS that reduce all species to elemental ions and may use calibration curves because of the elimination of matrix effects and molecular information due to harsh ionization.
- the anion chelators In a matrix with any other amount or mixture or component of metal ions (such as K or any metal ion that would be chelated by the CN " chelate) the anion chelators would express the Fe, Cu, Ni, Cd, Hg in the spectrum will be modified and will be completely different and is impossible to predict theoretically at the present time.
- metal ions such as K or any metal ion that would be chelated by the CN " chelate
- the actual sample will have a formation constant and stepwise formation constents with mathematical stabilities that are multiplicative such as for Hg2+ Cd2+ for Kl, K1K2, K1K2K3, K1K2K3K4 of 5.5, 5.1, 4.6, 3.6 for and 10.0, 16.7, 3.8 and 3.0 for the Log of Ks for Hg and Cd ions respectively with the cyanide negative ion. All of these would be in competition and are uncalculatable and must be measured with calibration cures being set up to quantify real samples impossible to predict. Only a calibrationless direct mathematical solution is possible in these complex soft ion molecular quantifications. There could be over 80 factorial possible combinations of ions that could possibly be expressed in a water solution.
- the direct mathematical calculation is the only reliable way to both identify and quantify the analytes(s) as calibration in the traditional sense is not applicable.
- NIST standard reference materials Rosin SRM 2704 and soil SRM 2711
- European IAEA CRM human hair IAEA-085
- SRMs 2704 and 2711
- Both SRMs were spiked with known amount of isotopically enriched inorganic mercury ( 199 Hg 2+ ).
- the sample preparation methods evaluated during this study were EPA Method 3052, EPA Draft Method 3200 (Microwave-Assisted Extraction, MAE) and EPA Method 3200 (Ultrasound-Assisted Extraction, UAE).
- MAE Microwave-Assisted Extraction
- UAE Ultrasound-Assisted Extraction
- Table 8 Human hair CRM (IAEA-085) certified for total mercury and methylmercury, and spiked with natural ethylmercury at a concentration of 22 ⁇ g/g.
- EPA Method 6800 was applied for three species and for all six conversions of species correction using microwave extraction and HPLC-ICP-MS analysis. Multiple replicates are shown along with analytical blank values that were obtained in this reduction-to-practice study.
- the analytical data produced by a mass spectrometer under BDMS and/or SIDMS protocols is a calibrated and highly accurate result.
- the DDMS and 1 SIDMS protocols were accepted by the US Environmental Protection Agency (EPA) as a national method under the designation, "Method 6800," which has been recognized in by the EPA and British Standards Institute (BSl) in published comments and documents as the only method capable of producing legally-defensible data for speciated elemental analysis.
- HMS Integrated Instrument-Method System
- IDMS and SIDMS products marketed by Applied Isotope Technologies (AIT) have been sold to environmental laboratories, research laboratories, industrial laboratories and Centers for Disease Control for the measurement of water, soil, hair, tissue, blood and urine analysis for toxic chemicals of natural and industrial origins.
- AIT products have been sold as IDMS and SIDMS kits that includes isotopic spikes, enriched standard analogues and software for the final mathematical deconvolution and calculation.
- AIT's products have been used in different types of mass spectrometers, such as Time-of-Flight (TOF) and Inductively Coupled Plasma (ICP), coupled to High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC), using both electrospray ionization (liquid-to-liquid spray) and gas ionization (liquid-to-gas) forms.
- TOF Time-of-Flight
- ICP Inductively Coupled Plasma
- HPLC High Performance Liquid Chromatography
- GC Gas Chromatography
- Additional inventions such as solid-phase media holding isotopic tags or enriched standard analogues, used for rapid spiking and equilibration, and simultaneous extraction, spiking and equilibration have been reduced to practice.
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AU2007349186A AU2007349186B2 (en) | 2006-12-07 | 2007-12-07 | Solid phase and catalyzed enabled automated isotope dilution and speciated isotope dilution mass spectrometry |
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Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011020187A1 (en) * | 2009-08-19 | 2011-02-24 | Mcgill University | Methods and systems for the quantitative chemical speciation of heavy metals and other toxic pollutants |
CN103913534B (en) * | 2014-02-11 | 2016-08-17 | 中国科学院地质与地球物理研究所兰州油气资源研究中心 | A kind of series hydrocarbon compound carbon isotope analysis method in natural gas |
CN103837593B (en) * | 2014-03-18 | 2016-11-23 | 中国计量科学研究院 | Isotope dilution mass spectrometry quantitative approach after a kind of human serum protein electrophoresis |
CN104006993B (en) * | 2014-05-26 | 2016-08-24 | 中国兵器工业集团第五三研究所 | The test sample preparation method of sulfur content in ID-ICP-MS method fuel oil |
US9443708B2 (en) * | 2014-09-10 | 2016-09-13 | Battelle Memorial Institute | Ion implantation system and process for ultrasensitive determination of target isotopes |
CN104897766B (en) * | 2015-04-27 | 2017-08-08 | 北京市医疗器械检验所 | The bearing calibration of trace element in a kind of use Isotope Dilution Mass Spectrometry sample |
US11385208B2 (en) * | 2016-03-07 | 2022-07-12 | Hitachi High-Tech Corporation | Analysis device |
US12099045B2 (en) | 2016-03-08 | 2024-09-24 | United States Of America As Represented By The Secretary Of The Air Force | Focusing agents and calibration transportability |
US11099165B1 (en) | 2016-03-08 | 2021-08-24 | United States Of America As Represented By The Secretary Of The Air Force | Focusing agents and methods of using same |
WO2017178453A1 (en) * | 2016-04-14 | 2017-10-19 | Roche Diagnostics Gmbh | Method for determining a concentration of a target analyte in a sample of bodily fluid |
CN107576748B (en) * | 2016-09-29 | 2019-07-12 | 宁波大学 | A method of using seven kinds of Polychlorinated biphenyls in ultrasonic wave added-Headspace-solid phase microextraction technology measurement soil |
USD827308S1 (en) | 2017-03-07 | 2018-09-04 | Paris Presents Incorporated | Makeup brush |
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003006946A2 (en) * | 2001-07-13 | 2003-01-23 | Metara, Inc. | Method and instrument for automated analysis of fluid-based processing systems |
FR2883977A1 (en) * | 2005-04-05 | 2006-10-06 | Centre Nat Rech Scient | Direct analysis laser machine for microelectronics industry, has ablation unit with femtosecond laser source, and laser beam displacement unit allowing displacement of laser beam on sample`s surface in two directions parallel to surface |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6113153A (en) * | 1984-06-29 | 1986-01-21 | Hitachi Ltd | Automatic titrating device |
US5414259A (en) * | 1994-01-05 | 1995-05-09 | Duquesne University Of The Holy Ghost | Method of speciated isotope dilution mass spectrometry |
UA44357C2 (en) * | 1996-03-15 | 2002-02-15 | Брітіш Н'Юклеа Ф'Юелз Піелсі | METHOD OF SEPARATION OF MATERIAL, WHICH CONSISTS OF DIFFERENT COMPONENTS, AND SEPARATION DEVICE |
US6790673B1 (en) * | 1998-01-29 | 2004-09-14 | Duquesne University Of The Holy Ghost | Speciated isotope dilution mass spectrometry of reactive species and related methods |
JP2004503780A (en) * | 2000-06-12 | 2004-02-05 | ユニバーシティ オブ ワシントン | Selective labeling and isolation of phosphopeptides and application to proteome analysis |
EP1330532B1 (en) * | 2000-10-03 | 2011-12-14 | Mirari Biosciences, Inc. | Methods and compositions for directed microwave chemistry |
US7348182B2 (en) * | 2000-10-03 | 2008-03-25 | Mirari Biosciences, Inc. | Directed microwave chemistry |
JP4062514B2 (en) * | 2001-01-02 | 2008-03-19 | ザ・クリーブランド・クリニック・ファンデーション | Myeloperoxidase, a risk indicator for cardiovascular disease |
CA2434094A1 (en) * | 2001-01-29 | 2002-08-08 | Metara, Inc. | Automated in-process isotope and mass spectrometry |
US7183116B2 (en) * | 2001-05-14 | 2007-02-27 | The Institute For Systems Biology | Methods for isolation and labeling of sample molecules |
US7531134B1 (en) * | 2002-03-08 | 2009-05-12 | Metara, Inc. | Method and apparatus for automated analysis and characterization of chemical constituents of process solutions |
-
2007
- 2007-12-07 DK DK07873961.2T patent/DK2108111T3/en active
- 2007-12-07 CN CN2013100318147A patent/CN103257070A/en active Pending
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- 2007-12-07 AU AU2007349186A patent/AU2007349186B2/en active Active
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- 2007-12-07 EP EP07873961.2A patent/EP2108111B1/en active Active
-
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- 2010-06-08 HK HK10105610.7A patent/HK1138908A1/en unknown
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003006946A2 (en) * | 2001-07-13 | 2003-01-23 | Metara, Inc. | Method and instrument for automated analysis of fluid-based processing systems |
FR2883977A1 (en) * | 2005-04-05 | 2006-10-06 | Centre Nat Rech Scient | Direct analysis laser machine for microelectronics industry, has ablation unit with femtosecond laser source, and laser beam displacement unit allowing displacement of laser beam on sample`s surface in two directions parallel to surface |
Non-Patent Citations (4)
Title |
---|
HEUMANN: "isotope-dilution ICP-MS for trace element determination and speciation: from a reference method to a routine method?" ANALYTICAL BIOANALYTICAL CHEMISTRY, vol. 378, 2004, pages 318-329, XP002495788 * |
RUIZ ENCINAR J ET AL: "Isotopically-labelled compounds for validating organometallics speciation analysis" TRAC, TRENDS IN ANALYTICAL CHEMISTRY, ELSEVIER, AMSTERDAM, NL, vol. 22, no. 2, 1 February 2003 (2003-02-01), pages 108-114, XP004413152 ISSN: 0165-9936 * |
SERGEI F BOULYGA ET AL: "Isotope dilution ICP-MS with laser-assisted sample introduction for direct determination of sulfur in petroleum products" ANALYTICAL AND BIOANALYTICAL CHEMISTRY, SPRINGER, BERLIN, DE, vol. 382, no. 8, 1 August 2005 (2005-08-01), pages 1808-1814, XP019327538 ISSN: 1618-2650 * |
TIBI ET AL: "idms as a calibration method for the analysis of trace elements in powder samples by LA-ICP-MS" JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, vol. 18, 2003, pages 1076-1081, XP002495806 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11022593B2 (en) | 2018-10-25 | 2021-06-01 | Savannah River Nuclear Solutions, Llc | Solid phase sampling device and methods for point-source sampling of polar organic analytes |
US11808750B2 (en) | 2018-10-25 | 2023-11-07 | Battelle Savannah River Alliance, Llc | Solid-phase sampling device and methods for point-source sampling of polar organic analytes |
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US20120142545A1 (en) | 2012-06-07 |
JP5412288B2 (en) | 2014-02-12 |
AU2007349186B2 (en) | 2014-03-06 |
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AU2007349186A1 (en) | 2008-09-18 |
CA2671859A1 (en) | 2008-09-18 |
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CN101600954A (en) | 2009-12-09 |
CN101600954B (en) | 2013-03-13 |
HK1138908A1 (en) | 2010-09-03 |
US8383420B2 (en) | 2013-02-26 |
CA2671859C (en) | 2016-07-12 |
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JP2010512515A (en) | 2010-04-22 |
DK2108111T3 (en) | 2019-07-15 |
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