WO2008106984A3 - Pig model for breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex - Google Patents

Pig model for breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex Download PDF

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Publication number
WO2008106984A3
WO2008106984A3 PCT/DK2008/050057 DK2008050057W WO2008106984A3 WO 2008106984 A3 WO2008106984 A3 WO 2008106984A3 DK 2008050057 W DK2008050057 W DK 2008050057W WO 2008106984 A3 WO2008106984 A3 WO 2008106984A3
Authority
WO
WIPO (PCT)
Prior art keywords
breast cancer
related protein
protein folding
epidermolysis bullosa
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DK2008/050057
Other languages
French (fr)
Other versions
WO2008106984A2 (en
Inventor
Peter Michael Kragh
Lars Axel Bolund
Charlotte Brandt Soerensen
Thomas Juhl Corydon
Niels Gregersen
Peter Bross
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aarhus Universitet
Original Assignee
Aarhus Universitet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aarhus Universitet filed Critical Aarhus Universitet
Priority to EP08715611A priority Critical patent/EP2134848A2/en
Priority to CA2717251A priority patent/CA2717251A1/en
Priority to US12/529,816 priority patent/US20100115636A1/en
Publication of WO2008106984A2 publication Critical patent/WO2008106984A2/en
Publication of WO2008106984A3 publication Critical patent/WO2008106984A3/en
Anticipated expiration legal-status Critical
Priority to US13/592,045 priority patent/US20130150465A1/en
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8778—Swine embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A01K2217/052—Animals comprising random inserted nucleic acids (transgenic) inducing gain of function
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0331—Animal model for proliferative diseases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Environmental Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention relates to a genetically modified pig as a model for studying breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex. The modified pig model displays one or more phenotypes associated with any of said disorders. Disclosed is also a modified pig comprising a modified endogeneous BRCA1 and/or BRCA 2 gene, and/or a modified ornithine transcarbamylase gene, and/or a modified Keratin 14 gene and/or a transcriptional or translational product or part thereof. The invention further relates to methods for producing the modified pig; and methods for evaluating the effect of a therapeutical treatment of breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex; methods for screening the efficacy of a pharmaceutical composition; and a method for treatment of a human being suffering from breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex are disclosed.
PCT/DK2008/050057 2007-03-07 2008-03-06 Pig model for breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex Ceased WO2008106984A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP08715611A EP2134848A2 (en) 2007-03-07 2008-03-06 Pig model for breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex
CA2717251A CA2717251A1 (en) 2007-03-07 2008-03-06 Pig model for breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex
US12/529,816 US20100115636A1 (en) 2007-03-07 2008-03-06 Pig model for breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex
US13/592,045 US20130150465A1 (en) 2007-03-07 2012-08-22 Pig model for breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DKPA200700344 2007-03-07
DKPA200700344 2007-03-07
DKPA200700342 2007-03-07
DKPA200700342 2007-03-07
DKPA200700341 2007-03-07
DKPA200700341 2007-03-07

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/592,045 Continuation US20130150465A1 (en) 2007-03-07 2012-08-22 Pig model for breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex

Publications (2)

Publication Number Publication Date
WO2008106984A2 WO2008106984A2 (en) 2008-09-12
WO2008106984A3 true WO2008106984A3 (en) 2008-12-31

Family

ID=39592108

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2008/050057 Ceased WO2008106984A2 (en) 2007-03-07 2008-03-06 Pig model for breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex

Country Status (4)

Country Link
US (2) US20100115636A1 (en)
EP (1) EP2134848A2 (en)
CA (1) CA2717251A1 (en)
WO (1) WO2008106984A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7989675B2 (en) * 2007-03-28 2011-08-02 University Of Iowa Research Foundation Method of identifying compounds using a transgenic pig model of cystic fibrosis
US8912386B2 (en) 2007-03-28 2014-12-16 University Of Iowa Research Foundation Transgenic pig model of cystic fibrosis
US8618352B2 (en) 2007-03-28 2013-12-31 University Of Iowa Research Foundation Transgenic porcine models of cystic fibrosis
US10172333B2 (en) 2013-03-15 2019-01-08 Exemplar Genetics, Llc Animal models of cancer
JP6929527B2 (en) * 2015-03-04 2021-09-01 株式会社ポル・メド・テック Disease model pigs showing stable phenotype and methods for producing them
GB201504124D0 (en) 2015-03-11 2015-04-22 Proqr Therapeutics B V Oligonucleotides
WO2017096218A1 (en) * 2015-12-03 2017-06-08 The Penn State Research Foundation Genomic regions with epigenetic variation that contribute to phenotypic differences in livestock
WO2022204370A1 (en) * 2021-03-24 2022-09-29 Modernatx, Inc. Lipid nanoparticles and polynucleotides encoding ornithine transcarbamylase for the treatment of ornithine transcarbamylase deficiency
CN115807036B (en) * 2022-08-15 2026-02-03 南京启真基因工程有限公司 Method for preparing BRCA2 gene mutation breast cancer model pig nuclear transplantation donor cells and special gene editing system thereof

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999060108A2 (en) * 1998-05-15 1999-11-25 Sri International Transgenic animals produced by homologous sequence targeting
US20020035736A1 (en) * 2000-03-16 2002-03-21 Sharon Erickson HER2-transgenic non-human tumor model
WO2002077027A1 (en) * 2000-03-23 2002-10-03 Curagen Corporation Human stra6-like protein and nucleic acids encoding the same
WO2003064633A1 (en) * 2002-01-11 2003-08-07 Gtc Biotherapeutics, Inc. Method and system for fusion and activation following nuclear transfer in reconstructed embryos
GB2388114A (en) * 2002-04-10 2003-11-05 Peter Maccallum Cancer Inst Methods of diagnosis or prognosis of a BRCA associated disorder
WO2004064768A2 (en) * 2003-01-23 2004-08-05 Agy Therapeutics, Inc. Animal model for alzheimer's disease
WO2007028396A2 (en) * 2005-09-08 2007-03-15 Aarhus Universitet Cell nuclear transfer
WO2007124751A2 (en) * 2006-05-01 2007-11-08 Aarhus Universitet An animal model and a method for producing an animal model

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1241931A4 (en) * 1999-09-13 2004-12-29 Univ Massachusetts CLONING OF PIGS USING CELLS OR DONOR CORES FROM DIFFERENTIATED CELLS AND PRODUCTION OF MULTIPOTENT SWINE

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999060108A2 (en) * 1998-05-15 1999-11-25 Sri International Transgenic animals produced by homologous sequence targeting
US20020035736A1 (en) * 2000-03-16 2002-03-21 Sharon Erickson HER2-transgenic non-human tumor model
WO2002077027A1 (en) * 2000-03-23 2002-10-03 Curagen Corporation Human stra6-like protein and nucleic acids encoding the same
WO2003064633A1 (en) * 2002-01-11 2003-08-07 Gtc Biotherapeutics, Inc. Method and system for fusion and activation following nuclear transfer in reconstructed embryos
GB2388114A (en) * 2002-04-10 2003-11-05 Peter Maccallum Cancer Inst Methods of diagnosis or prognosis of a BRCA associated disorder
WO2004064768A2 (en) * 2003-01-23 2004-08-05 Agy Therapeutics, Inc. Animal model for alzheimer's disease
WO2007028396A2 (en) * 2005-09-08 2007-03-15 Aarhus Universitet Cell nuclear transfer
WO2007124751A2 (en) * 2006-05-01 2007-11-08 Aarhus Universitet An animal model and a method for producing an animal model

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CAO T, ET AL.: "AN INDUCIBLE MOUSE MODEL FOR EPIDERMOLYSIS BULLOSA SIMPLEX: IMPLICATION FOR GENE THERAPY", THE JOURNAL OF CELL BIOLOGY, vol. 152, no. 3, 2001, pages 651 - 656, XP002500577 *
DATABASE EMBL 1 September 1996 (1996-09-01), XP002488550, Database accession no. Z75667 *
LLOYDS ET AL, ET AL.: "THE BASAL KERATIN NETWORK OF STRATIFIED SQUAMOUS EPITHELIA: DEFINING K15 FUNCTION IN THE ABSENCE OF K14", THE JOURNAL OF CELL BIOLOGY, vol. 129, no. 5, 1995, pages 1329 - 1344, XP002500576 *
PETERS B, ET AL.: "COMPLETE CYTOLYSIS AND NEONATAL LETHALITY IN KERATIN 5 KNOCKOUT MICE REVEALS ITS FUNDAMENTAL ROLE IN SKIN INTEGRITY AND IN EPIDERMOLYSIS BULLOSA SIMPLEX", MOLECULAR BIOLOGY, vol. 12, 2001, pages 1775 - 1789, XP002500578 *
VODICKA PETR ET AL: "The miniature pig as an animal model in biomedical research", ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, NEW YORK ACADEMY OF SCIENCES, NEW YORK, NY, US, vol. 1049, 1 May 2005 (2005-05-01), pages 161 - 171, XP009090232, ISSN: 0077-8923 *

Also Published As

Publication number Publication date
EP2134848A2 (en) 2009-12-23
CA2717251A1 (en) 2008-09-12
US20100115636A1 (en) 2010-05-06
WO2008106984A2 (en) 2008-09-12
US20130150465A1 (en) 2013-06-13

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