WO2008105824A3 - Détection directe d'interactions ligand-antiligand par une commutation à l'échelle nanométrique - Google Patents

Détection directe d'interactions ligand-antiligand par une commutation à l'échelle nanométrique Download PDF

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Publication number
WO2008105824A3
WO2008105824A3 PCT/US2007/019087 US2007019087W WO2008105824A3 WO 2008105824 A3 WO2008105824 A3 WO 2008105824A3 US 2007019087 W US2007019087 W US 2007019087W WO 2008105824 A3 WO2008105824 A3 WO 2008105824A3
Authority
WO
WIPO (PCT)
Prior art keywords
nanoparticles
conductive
ligand
detection
antiligand
Prior art date
Application number
PCT/US2007/019087
Other languages
English (en)
Other versions
WO2008105824A2 (fr
Inventor
Julian Gordon
Original Assignee
Nanosphere Inc
Julian Gordon
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanosphere Inc, Julian Gordon filed Critical Nanosphere Inc
Publication of WO2008105824A2 publication Critical patent/WO2008105824A2/fr
Publication of WO2008105824A3 publication Critical patent/WO2008105824A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Nanotechnology (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)

Abstract

L'invention concerne une méthodologie pour la création de capteurs et de microcircuits de structures sous la forme de feuilles, de films ou de fils, comportant un élément capteur, pour la détection directe d'interactions de liaison d'antiligand-ligand. Des nanostructures comportent des nanoparticules conductrices liées à un substrat non-conducteur, un réseau ordonné des nanoparticules ayant une densité inférieure au seuil sous lequel la nanostructure devient conductrice. Des antiligands sont fixés sur les nanoparticules du substrat et un ensemble correspondant de ligands parents sont fixés sur un ensemble supplémentaire de nanoparticules de détection, respectivement. Les nanoparticules de détection sont également conductrices. La réaction de liaison est déterminée à partir de la transition dans les propriétés électriques des structures, favorisée par la liaison des nanoparticules de détection conductrices. Les paires de ligand-antiligand peuvent être des paires d'anticorps-antigène ou des ensembles correspondants d'acides nucléiques ou d'oligonucléotides complémentaires. Une structure utile peut en outre être créée au moyen de formats de type sandwich impliquant des paires additionnelles d'interactions de liaison.
PCT/US2007/019087 2006-08-29 2007-08-29 Détection directe d'interactions ligand-antiligand par une commutation à l'échelle nanométrique WO2008105824A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US84106806P 2006-08-29 2006-08-29
US60/841,068 2006-08-29
US87603506P 2006-12-20 2006-12-20
US60/876,035 2006-12-20

Publications (2)

Publication Number Publication Date
WO2008105824A2 WO2008105824A2 (fr) 2008-09-04
WO2008105824A3 true WO2008105824A3 (fr) 2008-10-23

Family

ID=39721720

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/019087 WO2008105824A2 (fr) 2006-08-29 2007-08-29 Détection directe d'interactions ligand-antiligand par une commutation à l'échelle nanométrique

Country Status (1)

Country Link
WO (1) WO2008105824A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010096331A1 (fr) * 2009-02-11 2010-08-26 Duke University Capteurs incorporant des anticorps et leurs procédés de fabrication et d'utilisation
DE112009005088A5 (de) * 2009-07-21 2012-10-04 Heiko Schwertner Verfahren unter der verwendung von non impact druckmethoden zusammen mit druckflüssigkeiten, die aktive biologische moleküle enthalten, zur produktion von sensoren und komplexen analytischen systemen.
US10407716B2 (en) 2014-03-13 2019-09-10 Duke University Electronic platform for sensing and control of electrochemical reactions
KR102592972B1 (ko) * 2016-02-12 2023-10-24 삼성전자주식회사 센싱 모듈 기판 및 이를 포함하는 센싱 모듈

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030207296A1 (en) * 1996-07-29 2003-11-06 So-Jung Park Nanoparticles having oligonucleotides attached thereto and uses therefor
US20050064395A1 (en) * 2003-07-25 2005-03-24 Platypus Technologies, Llc Liquid crystal based analyte detection
US20060068378A1 (en) * 1996-07-29 2006-03-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030207296A1 (en) * 1996-07-29 2003-11-06 So-Jung Park Nanoparticles having oligonucleotides attached thereto and uses therefor
US20060068378A1 (en) * 1996-07-29 2006-03-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US20050064395A1 (en) * 2003-07-25 2005-03-24 Platypus Technologies, Llc Liquid crystal based analyte detection

Also Published As

Publication number Publication date
WO2008105824A2 (fr) 2008-09-04

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